We narrowed to 57,330 results for: plasmid
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Plasmid#57818PurposeLentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses eGFP via P2A cleavage site. EFS Promoter drivenDepositorInsertsUseCRISPR and LentiviralTagsFLAGPromoterEFS and hU6Available sinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by
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pCX-OKS-2A
Plasmid#19771PurposeNon-integrating (episomal) expression of mouse Oct3/4, Klf4 and Sox2DepositorExpressionMammalianMutationThree genes are connected with the foot-and-mouth…Available sinceJan. 5, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pC016 - LwCas13a guide expression backbone with U6 promoter
Plasmid#91906PurposeBackbone for cloning LwCas13a guides under U6 promoterDepositorHas serviceCloning Grade DNAInsertLwCas13a Guide site
ExpressionMammalianAvailable sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSin-EF2-LIN28-Pur
Plasmid#16580DepositorAvailable sinceJan. 4, 2008AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-GFP
Plasmid#71237PurposeExpress sgRNA and dCas9-KRAB from 3rd generation lentiviral vectorDepositorInsertshumanized dCas9-KRAB T2A GFP
sgRNA
UseCRISPR and LentiviralTagsFlagMutationD10A and H840AAvailable sinceDec. 7, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
p3TP-lux
Plasmid#11767DepositorPublicationInsert3TP
UseLuciferaseExpressionMammalianAvailable sinceAug. 4, 2006AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pEP4 E02S CK2M EN2L
Plasmid#20924Purposeused in the derivation of human iPS cells using non-integrating episomal vectors; expresses Oct4 and Sox2; Klf4 and Myc; Nanog and Lin28DepositorPublicationUseEpisomal/ebnaAvailable sinceMay 20, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
FUW-tetO-hMYC
Plasmid#20723DepositorAvailable sinceMarch 13, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pX330-BbsI-PITCh
Plasmid#127875PurposeFor user to clone their gRNA oligos to BbsI site. PITCh gRNA expression cassette is already build in so there is no need to perform Golden Gate assemblyDepositorTypeEmpty backboneUseCRISPRExpressionMammalianAvailable sinceJuly 8, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAc-sgRNA-Cas9
Plasmid#49330PurposeExpresses sgRNA and Cas9-Puro in Drosophila S2 cellsDepositorInsertsCas9
dU6-sgRNA
UseCRISPRTags3xFLAG and NLSExpressionInsectMutationHuman codon optimisedPromoterActin-5c and Drosophila U6Available sinceDec. 19, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
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IFN-Beta_pGL3
Plasmid#102597PurposeIFN-beta promoter driving luciferase. Use in IFN reporter assay.DepositorAvailable sinceJan. 18, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pC0046-EF1a-PspCas13b-NES-HIV
Plasmid#103862PurposeExpresses PspCas13b in mammalian cells for knockdown of target RNAs in combination with compatible guide RNAs. Localized to the cytoplasmDepositorInsertPspCas13b
UseCRISPR and LentiviralTags3xHA and HIV NESExpressionMammalianAvailable sinceNov. 28, 2017AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pT7-gRNA
Plasmid#46759PurposegRNA cloning vector for in vitro transcription of target gRNADepositorInsertgRNA core
UseCRISPR; Zebrafish expressionPromoterT7Available sinceAug. 2, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV.hSynapsin.SF-iGluSnFR.A184S
Plasmid#106174PurposeTight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorHas serviceAAV1 and AAV9InsertSF-iGluSnFR.A184S
UseAAVMutationGltI: A184SPromoterhSynapsinAvailable sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCRISPomyces-2
Plasmid#61737PurposeStreptomyces expression of codon-optimized Cas9 and custom gRNADepositorPublicationInsertssSpCas9
gRNA cassette
UseCRISPRExpressionBacterialMutationBbsI-flanked lacZ cassette inserted in place of s…Promotergapdhp(EL) and rpsL(XC)-BbsIAvailable sinceJan. 27, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSyn-DIO-EGFP
Plasmid#50457PurposeDouble floxed EGFP under the control of human synapsin promoterDepositorHas serviceAAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9InsertEGFP
UseAAVTagsN/APromoterhuman Synapsin 1Available sinceMarch 17, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-CaMKIIa-EGFP
Plasmid#50469PurposeEGFP under the control of CaMKIIa promoterDepositorHas serviceAAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9InsertEGFP
UseAAVTagsN/APromoterCaMKIIaAvailable sinceMarch 17, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
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pBS598 EF1alpha-EGFPcre
Plasmid#11923PurposeExpresses EGFP-Cre fusion under the control of the EF1alpha promoter; EGFP is human codon-optimized.DepositorPublicationAvailable sinceMarch 23, 2007AvailabilityAcademic Institutions and Nonprofits onlyCited by