4,143 results

1. pnCasPA-BEC

   Plasmid

   #113349

   Purpose

   A cytidine deaminase-mediated base-editing plasmid in Pseudomonas species

   Depositor

   Quanjiang Ji

   Article

   Chen et al iScience. 2018 Aug 31;6:222-231. doi: 10

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. hAAVS1 1R TALEN

   Plasmid

   #35432

   Purpose

   TALEN designed to target the human AAVS1 locus

   Depositor

   Feng Zhang

   Article

   Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

   Insert

   hAAVS1 1R TALEN
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 10, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. hAAVS1 1L TALEN

   Plasmid

   #35431

   Purpose

   TALEN designed to target the human AAVS1 locus

   Depositor

   Feng Zhang

   Article

   Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

   Insert

   hAAVS1 1L TALEN
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHR-SFFV-dCas9-BFP-KRAB

   Plasmid

   #46911

   Purpose

   Human expression vector containing SFFV promoter, dCas9 that is fused to 2x NLS, tagBFP and a KRAB domain

   Depositor

   Stanley Qi, Jonathan Weissman

   Article

   Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

   Insert

   dCas9-BFP-KRAB fusion
   

   Use

   CRISPR and Lentiviral

   Tags

   2xNLS, BFP, HA, and KRAB domain

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   Oct. 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. HA-TAZ

   Plasmid

   #32839

   Depositor

   Kunliang Guan

   Article

   Lei et al Mol Cell Biol. 2008 Apr;28(7):2426-36. E

   Insert

   TAZ (WWTR1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 15, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV-EF1a-IRES-Hygro

   Plasmid

   #85134

   Purpose

   lentiviral backbone

   Depositor

   Tobias Meyer

   Article

   Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDest-566

   Plasmid

   #11517

   Depositor

   Dominic Esposito

   Type

   Empty backbone

   Use

   Gateway destination vector

   Tags

   6xHis and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7-lacO (lactose/IPTG inducible)

   Available since

   May 24, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-PE6b

   Plasmid

   #207852

   Purpose

   Mammalian expression of PE6b prime editor

   Depositor

   David Liu

   Article

   Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

   Insert

   PE6b
   

   Use

   Tags

   SV40 bpNLS and SV40 bpNLS, c-Myc NLS

   Expression

   Mammalian

   Mutation

   Tf1rt(P70T, G72V, S87G, M102I, K106R, K118R, I128…

   Promoter

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-FLEX-tdTomato

   Plasmid

   #28306

   Depositor

   Edward Boyden

   Has Service

   AAV PHP.S, AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

   Insert

   tdTomato
   

   Use

   AAV and Cre/Lox; Adeno-associated virus

   Tags

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   CAG

   Available since

   Sept. 7, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMSCV-IRES-mCherry FP

    Plasmid

    #52114

    Purpose

    a bicistronic IRES-mCherry containing retroviral vector

    Depositor

    Dario Vignali

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    IRES-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-puro-ARID1A

    Plasmid

    #39478

    Purpose

    Tetracycline-inducible lentiviral expression of human ARID1A; 3rd generation lentiviral vector

    Depositor

    Ie-Ming Shih

    Article

    Guan et al Cancer Res. 2011 Nov 1;71(21):6718-27. E

    Insert

    ARID1A (ARID1A Human)
    

    Use

    Lentiviral

    Tags

    His and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLVX-UbC-rtTA-Ngn2:2A:Ascl1

    Plasmid

    #127289

    Purpose

    all-in-one inducible LV expression construct for Ngn2:2A:Ascl1 (aka UNA; for direct neuronal conversion)

    Depositor

    Fred Gage

    Article

    Herdy et al Elife. 2019 May 17;8. pii: 41356. doi: 1

    Inserts

    Ngn2:2A:Ascl1 (ASCL1, NEUROG2 Human)
    Ngn2:2A:Ascl1 (ASCL1, NEUROG2 Human)
    

    Use

    Lentiviral

    Tags

    Ngn2 and Ascl1 linked via 2A

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. 7M8

    Plasmid

    #64839

    Purpose

    This plasmid can be used in the triple transfection method of AAV vector production. It supplies the replication proteins from AAV2 and the 7M8 capsid protein.

    Depositor

    John Flannery, David Schaffer

    Article

    Dalkara et al Sci Transl Med. 2013 Jun 12;5(189):189ra

    Insert

    7M8 cap
    

    Use

    AAV

    Tags

    Expression

    Mutation

    7mer insertion in the AAV2 cap

    Promoter

    Available since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. mCherry-Tsg101

    Plasmid

    #21505

    Depositor

    James Hurley

    Article

    Lee et al Science. 2008 Oct 24. 322(5901):576-80.

    Insert

    Tsg101 (TSG101 Human)
    

    Use

    Tags

    Cherry*

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Available since

    Sept. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. LAMP1-mGFP

    Plasmid

    #34831

    Depositor

    Esteban Dell'Angelica

    Article

    Falcon-Perez et al J Cell Sci. 2005 Nov 15;118(Pt 22):5243-

    Insert

    LAMP1 (LAMP1 Human)
    

    Use

    Tags

    GSTGSTGSTGA linker, Kozak, and mGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. mClY-N1

    Plasmid

    #90457

    Purpose

    A YFP-based chloride sensor with enhanced chloride sensitivity, photostability and reduced pH interference. The sequence of mClY is EYFP-F46L/Q69K/H148Q/I152L/V163S/S175G/S205V/A206K.

    Depositor

    Joseph Santos-Sacchi

    Article

    Zhong et al PLoS One. 2014 Jun 5;9(6):e99095. doi: 1

    Insert

    mClY-N1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    EYFP-F46L/Q69K/H148Q/I152L/V163S/S175G/S205V/A206…

    Promoter

    Available since

    May 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-GFP(1-10)

    Plasmid

    #70219

    Purpose

    Expresses GFP(1-10) in mammalian cells

    Depositor

    Bo Huang

    Article

    Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

    Has Service

    Cloning Grade DNA

    Insert

    GFP(1-10)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCIN BRCA2 WT

    Plasmid

    #16245

    Depositor

    Mien-Chie Hung

    Article

    Wang et al Cancer Res. 2002 Mar 1. 62(5):1311-4.

    Insert

    BRCA2 (BRCA2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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19. Lenti-sh1368 knockdown c-myc

    Plasmid

    #29435

    Depositor

    Bob Eisenman

    Article

    Lin et al EMBO J. 2009 Oct 21. 28(20):3157-70.

    Insert

    c-Myc shRNA (MYC Human)
    

    Use

    Lentiviral and RNAi

    Tags

    GFP and WPRE

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. CD63-pEGFP C2

    Plasmid

    #62964

    Purpose

    full length human CD63 cloned into pEGFP C2

    Depositor

    Paul Luzio

    Insert

    CD63 molecule (CD63 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    CD63 has 1 silent mutation compared to the coding…

    Promoter

    CMV

    Available since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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