8,717 results

1. ND4-DdCBE-right side TALE

   Plasmid

   #157843

   Purpose

   expresses right-side TALE–DddAtox half ND4-DdCBE in mammalian cells

   Depositor

   David Liu

   Article

   Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

   Insert

   COX8A MTS–3xFLAG–ND4 right TALE–G1397 DddA-N–UGI–ATP5B 3'UTR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. GFP-Dcp1a

   Plasmid

   #153972

   Purpose

   Fluorescent protein fused to mRNA decapping enzyme 1a

   Depositor

   Gia Voeltz

   Article

   Lee et al Science. 2020 Jan 31;367(6477). pii: 367

   Insert

   mRNA decapping enzyme 1a (DCP1A Human)
   

   Use

   Tags

   pAcGFP-C1

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pZW1-snoVector

   Plasmid

   #73174

   Purpose

   Nuclear expression of RNAs

   Depositor

   Ling-Ling Chen

   Article

   Yin et al Nucleic Acids Res. 2015 Jan;43(1):e5. do

   Inserts

   SNORD116-13 (SNORD116-13 Human)
   SNORD116-14 (SNORD116-14 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMax_GFP

   Plasmid

   #177825

   Purpose

   Host Cell Reactivation (HCR) control GFP plasmid (pMax backbone)

   Depositor

   Leona Samson, Zachary Nagel

   Article

   Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

   Insert

   GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSin Capsid

   Plasmid

   #127693

   Purpose

   Plasmid contains the Sindbis capsid gene and can be converted to mRNA for initiating Sindbis virus packaging. See Resource Information section for annotated plasmid sequence.

   Depositor

   Justin English, Bryan Roth

   Article

   English et al Cell. 2019 Jul 2. pii: S0092-8674(19)306

   Insert

   Sindbis Capsid
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 3, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. GW1-PercevalHR

   Plasmid

   #49082

   Purpose

   Mammalian expression vector (CMV promoter) of PercevalHR, a fluorescent sensor of ATP-to-ADP ratio.

   Depositor

   Gary Yellen

   Article

   Tantama et al Nat Commun. 2013;4:2550. doi: 10.1038/nc

   Insert

   PercevalHR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCRIS-PITChv2-Puro-dTAG (BRD4)

   Plasmid

   #91793

   Purpose

   PITCh dTAG donor vector for Puro-P2A-2xHA-FKBP_F36V knock-in into the N-terminus of the human BRD4 locus.

   Depositor

   Behnam Nabet, James Bradner

   Article

   Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

   Insert

   Puro-P2A-2xHA-FKBP_F36V
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Phenylalanine 36 to valine in FKBP12

   Promoter

   Promotorless

   Available since

   March 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. Lact-C2-GFP

   Plasmid

   #22852

   Depositor

   Sergio Grinstein

   Article

   Yeung et al Science. 2008 Jan 11. 319(5860):210-3.

   Insert

   Lactadherin-C2 Domain (MFGE8 Bovine)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 15, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. E-cadherin-GFP

   Plasmid

   #28009

   Depositor

   Jennifer Stow

   Article

   Miranda et al J Biol Chem. 2001 Jun 22. 276(25):22565-

   Insert

   E-cadherin (CDH1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCW57-MCS1-P2A-MCS2 (GFP)

    Plasmid

    #80924

    Purpose

    All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.

    Depositor

    Adam Karpf

    Article

    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

    Type

    Empty backbone

    Use

    Lentiviral; Doxycycline inducible; p2a self cleav…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Aug. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. dCas9-VPR_P2A_mCherry

    Plasmid

    #154193

    Purpose

    Lentiviral expression plasmid encoding dCas9-VPR and a P2A linked mCherry marker

    Depositor

    Anna Obenauf

    Article

    Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

    Insert

    sp-dCas9-VPR_P2A_mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    EF1as

    Available since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET-dCas9-VP64-6xHis

    Plasmid

    #62935

    Purpose

    Expression of dCas9-VP64-6xHis in bacterial cells

    Depositor

    David Liu

    Article

    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

    Insert

    dCas9-VP64
    

    Use

    Tags

    3xFLAG, 6xHis, NLS, and VP64

    Expression

    Bacterial

    Mutation

    D10A and H840A amino acid changes render Cas9 nuc…

    Promoter

    T7

    Available since

    May 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSRE SSG

    Plasmid

    #127692

    Purpose

    Plasmid expresses the Sindbis Structural Genome (SSG) via the serum response element (SRE) promoter. See Resource Information section for annotated plasmid sequence.

    Depositor

    Justin English, Bryan Roth

    Article

    English et al Cell. 2019 Jul 2. pii: S0092-8674(19)306

    Insert

    Sindbis Structural Genome
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 3, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pXR001: EF1a-CasRx-2A-EGFP

    Plasmid

    #109049

    Purpose

    CasRx (NLS-RfxCas13d-NLS) with 2A-EGFP for RNA targeting

    Depositor

    Patrick Hsu

    Article

    Konermann et al Cell. 2018 Mar 8. pii: S0092-8674(18)302

    Insert

    CasRx
    

    Use

    CRISPR and Lentiviral

    Tags

    2A-eGFP, HA, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    May 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET15b-AavLEA1

    Plasmid

    #53093

    Purpose

    AavLEA1 expression (in BL21 DE3 E. coli strain)

    Depositor

    Claude Férec

    Article

    Tripathi et al PLoS One. 2014 Aug 13;9(8):e104970. doi:

    Insert

    AavLEA1
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLVX-XBP1 mNeonGreen NLS

    Plasmid

    #115968

    Purpose

    ER-stress sensor - XBP1 splicing fluorescent reporter with mNeonGreen

    Depositor

    David Andrews

    Article

    Nougarede et al Assay Drug Dev Technol. 2018 Aug 8. doi:

    Insert

    X-box binding protein 1 (XBP1 Human, Synthetic)
    

    Use

    Lentiviral

    Tags

    HA tag, c-myc NLS, and mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLVX-puro-cOVA

    Plasmid

    #135073

    Purpose

    Expresses chicken cytoplasmic ovalbumin in lentiviral vector with puromycin resistance

    Depositor

    Maria Castro

    Insert

    cOVA (OVAL Chicken)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFA6a-6xGLY-3xFLAG-kanMX6

    Plasmid

    #20754

    Depositor

    Mark Hochstrasser

    Article

    Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

    Type

    Empty backbone

    Use

    Pcr-based c-terminal tagging

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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19. EGFP-alphasynuclein-A53T

    Plasmid

    #40823

    Depositor

    David Rubinsztein

    Article

    Furlong et al Biochem J. 2000 Mar 15;346 Pt 3:577-81.

    Insert

    SNCA (SNCA Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    A53T (aggregates more rapidly than the wild-type …

    Promoter

    CMV

    Available since

    May 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. HA Raptor

    Plasmid

    #8513

    Depositor

    David Sabatini

    Article

    Kim et al Cell 2002 Jul 26;110(2):163-75.

    Insert

    Raptor (RPTOR Human)
    

    Use

    Tags

    ha

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge