165,744 results

1. pAAV-UBC-XRI-HA

   Plasmid

   #178056

   Purpose

   Expression Recording Islands (XRIs) for recording cellular physiological histories along intracellular protein self-assembly. Contains XRI subunit with HA tag, under UBC promoter.

   Depositor

   Edward Boyden

   Article

   Linghu et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   XRI-HA
   

   Use

   AAV

   Tags

   HA-MBP

   Expression

   Mammalian

   Promoter

   Human ubiquitin C (UBC) promoter

   Available Since

   Oct. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. M23: pHR-SFFVp-FUSN::mCherry::SspB

   Plasmid

   #122148

   Purpose

   N terminal domain of FUS (1-214) tagged with SspB and mCherry.

   Depositor

   Clifford Brangwynne

   Article

   Bracha et al Cell. 2018 Nov 29;175(6):1467-1480.e13.

   Insert

   FUS (FUS Human)
   

   Use

   Lentiviral

   Tags

   mCherry-sspB

   Expression

   Mammalian

   Mutation

   Contains only amino acids 1-214

   Promoter

   SFFV

   Available Since

   March 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSPgRNA

   Plasmid

   #47108

   Purpose

   Expresses a S. pyogenes Cas9/dCas9 guide RNA in mammalian cells

   Depositor

   Charles Gersbach

   Article

   Perez-Pinera et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

   Has Service

   Cloning Grade DNA

   Insert

   SPgRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG G-CEPIA1er

   Plasmid

   #105012

   Purpose

   ER calcium sensor

   Depositor

   Franck Polleux

   Article

   Hirabayashi et al Science. 2017 Nov 3;358(6363):623-630. d

   Insert

   G-CEPIA1er
   

   Expression

   Mammalian

   Promoter

   pCAG

   Available Since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. PB-Ef1a-PP7 coat protein-Halo-GB1x3-WPRE

   Plasmid

   #198337

   Purpose

   PB plasmid expressing Halo-tagged PP7 coat protein

   Depositor

   Michael Ward

   Article

   De Pace et al Nat Neurosci. 2024 Apr 10. doi: 10.1038/

   Insert

   PP7 coat protein 3x Halo-GB1
   

   Use

   Piggybac

   Tags

   HaloTag

   Expression

   Mammalian

   Promoter

   Ef1a

   Available Since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR221-FURIN

   Plasmid

   #158442

   Purpose

   Gateway Cloning compatible entry vector for the human FURIN gene.

   Depositor

   Alejandro Chavez, Sho Iketani

   Insert

   FURIN (FURIN Human)
   

   Use

   Gateway entry vector

   Available Since

   Aug. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRDA_052

   Plasmid

   #136474

   Purpose

   Lentiviral expression of AsCas12a gRNA

   Depositor

   John Doench

   Article

   DeWeirdt et al Nat Biotechnol. 2020 Jul 13. pii: 10.103

   Insert

   Puromycin resistance
   

   Use

   Lentiviral and Synthetic Biology

   Promoter

   EF1a

   Available Since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-Lyso-TORCAR

   Plasmid

   #64929

   Purpose

   Lysosome-targeted FRET biosensor for monitoring mTORC1 kinase activity; targeted to cytosolic face of lysosomal membrane.

   Depositor

   Jin Zhang

   Article

   Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

   Insert

   LAMP1-TORCAR (LAMP1 Human, Synthetic)
   

   Tags

   Cerulean, Full-length LAMP1, and YPet

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCS2-nCas9n

   Plasmid

   #47929

   Purpose

   expression of an optimized Cas9 for genome-editing in zebrafish

   Depositor

   Wenbiao Chen

   Article

   Jao et al Proc Natl Acad Sci U S A. 2013 Aug 5.

   Insert

   nls-zcas9-nls
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. p11d-tRPA(123)

    Plasmid

    #102613

    Purpose

    Expression of human RPA in E. coli

    Depositor

    Marc Wold

    Article

    Henricksen et al J Biol Chem. 1994 Apr 15;269(15):11121-3

    Inserts

    RPA1 (RPA1 Human)
    RPA2 (RPA2 Human)
    RPA3 (RPA3 Human)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. UAS:mCherry

    Plasmid

    #170994

    Purpose

    UAS:GAL4 repeat-containing promoter drives simple mCherry reporter gene. Blasticidin selectable.

    Depositor

    Alice Ting

    Article

    Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

    Insert

    UAS:mCherry
    

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Promoter

    Synthetic 14x UAS:Gal4

    Available Since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pICH47742:: pRPS5a-PE2max-NC::tHSP

    Plasmid

    #227694

    Purpose

    Golden Gate level 1, position 2, PE2max-NC expression cassette for Arabidopsis.

    Depositor

    Jae-Yean Kim

    Article

    Vu et al Nat Plants. 2024 Sep 6. doi: 10.1038/s41

    Insert

    PE2max-NC being driven by pRPS5a and terminated by tHSP
    

    Use

    CRISPR

    Expression

    Plant

    Promoter

    pRPS5a

    Available Since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAVS1-TetOn-ZIM3-KRAB-dCas9-P2A-mCherry

    Plasmid

    #212829

    Purpose

    Doxycycline-inducible (Zim3)KRAB-dCas9-HA-P2A-mCherry cassette for integration at the AAVS1-locus of the human genome.

    Depositor

    Wolfgang Enard

    Article

    Edenhofer et al iScience. 2024 May 23;27(6):110090. doi:

    Insert

    ZIM3-KRAB-dCas9-P2A-mCherry
    

    Tags

    HA-tag and ZIM3-KRAB

    Expression

    Bacterial and Mammalian

    Promoter

    CAG, TetOn

    Available Since

    June 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSBtet-GP

    Plasmid

    #60495

    Purpose

    SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of GFP, rtTA and puromycin resistance gene

    Depositor

    Eric Kowarz

    Article

    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Transposon

    Expression

    Mammalian

    Promoter

    TCE & RPBSA

    Available Since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET-pA-Hia5

    Plasmid

    #174372

    Purpose

    SEE DEPOSITOR COMMENTS BELOW. Plasmid for IPTG-inducible expression and purification of protein A-Hia5-6xHis (pA-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)

    Depositor

    Aaron Straight

    Article

    Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi

    Insert

    protein A - hia5
    

    Tags

    6xHis and Protein A

    Expression

    Bacterial

    Available Since

    Sept. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA C12 ecDHFR DD YFP HA

    Plasmid

    #192815

    Purpose

    Expresses an N-terminal enhanced destabilizing domain (DD) version of E. coli DHFR with higher basal turnover in mammalian systems. Contains missense mutations W74R/T113S/E120D/Q146L.

    Depositor

    John Hulleman

    Article

    Nakahara et al ACS Chem Biol. 2022 Sep 19. doi: 10.1021

    Insert

    E. coli dihydrofolate reductase
    

    Tags

    enhanced yellow fluorescent protein and hemagglut…

    Expression

    Mammalian

    Mutation

    W74R/T113S/E120D/Q146L

    Promoter

    CMV

    Available Since

    Nov. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. L4440

    Plasmid

    #1654

    Depositor

    Andrew Fire

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    RNAi

    Tags

    T7p, T7p, lacZN, OriF1>>, OriF1<<

    Expression

    Worm

    Available Since

    March 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-PE2-P2A-GFP

    Plasmid

    #132776

    Purpose

    Prime editing in mammalian cells with co-translational GFP expression

    Depositor

    David Liu

    Article

    Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

    Insert

    PE2-P2A-GFP
    

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    See manuscript

    Promoter

    CMV

    Available Since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-CMV-FLEX-SaCas9-U6-sgSlc32a1

    Plasmid

    #159905

    Purpose

    Mutagenesis of Slc32a1

    Depositor

    Larry Zweifel

    Insert

    Slc32a1 (Slc32a1 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pZac2.1-GfaABC1D-TurboID(C )-HA-GPI

    Plasmid

    #163697

    Purpose

    Expresses c-terminal half of TurboID on surface of astrocytes

    Depositor

    Scott Soderling

    Article

    Takano et al Nature. 2020 Dec;588(7837):296-302. doi:

    Insert

    GPI anchored TurboID
    

    Use

    AAV

    Available Since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. OMMGFP 1-10

    Plasmid

    #164119

    Purpose

    Expresses the non-fluorescent GFP1-10 moiety on the cytosolic face of the OMM

    Depositor

    Tito Calì, Marisa Brini

    Article

    Cieri et al Cell Death Differ. 2018 Jun;25(6):1131-1

    Insert

    OMMGFP 1-10
    

    Expression

    Mammalian

    Available Since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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22. pBI-CMV1-mir451-CMV2-mir1

    Plasmid

    #164635

    Purpose

    Express miR-451 and miR-1-1 in mammalian cells

    Depositor

    David Bartel

    Article

    Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

    Inserts

    miR451a (MIR451A Human)
    miR-1-1 (MIR1-1 )
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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23. pT7-hMLH1dn for IVT

    Plasmid

    #178114

    Purpose

    Template for in vitro transcription of human MLH1dn

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    human MLH1dn (codon optimized) (MLH1 Human)
    

    Use

    Template for in vitro transcription

    Mutation

    Detailed in manuscript

    Promoter

    T7 (inactivated)

    Available Since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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24. pSI-Check2-hRluc-NFkB-firefly

    Plasmid

    #106979

    Purpose

    Luciferase reporter with internal control for NFkB signal.

    Depositor

    Qing Deng

    Article

    Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

    Insert

    NFKB recognition sites (NFKB1 Human)
    

    Use

    Luciferase

    Available Since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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25. DsRed-rab11 WT

    Plasmid

    #12679

    Depositor

    Richard Pagano

    Article

    Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

    Insert

    RAB11A (Homo sapiens) (RAB11A Human)
    

    Tags

    DsRed and HA

    Expression

    Mammalian

    Available Since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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26. pCMV-Cre

    Plasmid

    #123133

    Purpose

    Mammalian expression vector for wild-type Cre.

    Depositor

    David Liu

    Article

    Bessen et al Nat Commun. 2019 Apr 26;10(1):1937. doi:

    Insert

    Cre recombinase
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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27. mCherry-CD9-10

    Plasmid

    #55013

    Purpose

    Localization: Tetraspanin/Membrane, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    CD9 (CD9 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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28. pRSETB/mStayGold

    Plasmid

    #212017

    Purpose

    mStayGold has an n1 adaptor at its N-terminus and is useful for C-terminal tagging. This construct is used also for expression of mStayGold itself.

    Depositor

    Atsushi Miyawaki

    Article

    Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

    Insert

    mStayGold
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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29. pDD143

    Plasmid

    #119880

    Purpose

    Arabinose inducible dCas9sth1 and constitutive sgRNA on pAL5000/pMB1 backbone with gentamicin resistance.

    Depositor

    Tae Seok Moon

    Article

    DeLorenzo et al ACS Synth Biol. 2018 Feb 16;7(2):727-738

    Insert

    pBAD_dCas9sth1
    

    Expression

    Bacterial

    Available Since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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30. PB-TO-hNGN2

    Plasmid

    #172115

    Purpose

    Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression

    Depositor

    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

    Article

    Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1

    Inserts

    hNGN2 (NEUROG2 Human)
    puroR-T2A-mycNLS-mTagBFP2
    

    Tags

    T2A-mycNLS-mTagBFP2

    Expression

    Mammalian

    Promoter

    EF1a and TRE3G

    Available Since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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