164,578 results

1. pT040

   Plasmid

   #67640

   Purpose

   URA3 vector for cloning guide sequences using BclI-SwaI sites into guide RNA expression cassette

   Depositor

   John Wyrick

   Article

   Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1

   Insert

   single guide RNA expression cassette
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   pSNR52

   Available Since

   Aug. 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKAR2-Br512

   Plasmid

   #161875

   Purpose

   Expresses Br512 polymerase in E.coli

   Depositor

   Andrew Ellington

   Article

   Maranhao et al medRxiv

   Insert

   Modified DNA Polymerase I Large Fragment (Br512)
   

   Use

   Tags

   8x His tag; Villin Headpiece 47 amino acid (HP47)…

   Expression

   Bacterial

   Mutation

   Promoter

   T7, tetPA

   Available Since

   Nov. 11, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. qTAG-AAVS1-Ef1a-Blast

   Plasmid

   #227267

   Purpose

   Empty AAVS1 targeting donor for the insertion of Blast and a strong EF1a promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. mNeonGreen-Rab7

   Plasmid

   #129603

   Purpose

   In vivo visualization of Rab7 (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

   Insert

   Rab7
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAEMXT-eLACCO1.1

   Plasmid

   #167946

   Purpose

   Fluorescent biosensor for extracellular L-lactate

   Depositor

   Robert Campbell

   Article

   Nasu et al Nat Commun. 2021 Dec 6;12(1):7058. doi:

   Insert

   CD59-eLACCO1.1-GPI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pIND-HA-ADH

   Plasmid

   #11991

   Depositor

   Steven Johnson

   Insert

   aldehyde dehydrogenase (ALDH1A1 Human)
   

   Use

   Ecdysone inducible

   Tags

   HA

   Expression

   Mammalian

   Mutation

   no mutations

   Promoter

   Available Since

   July 25, 2007

   Availability

   Academic Institutions and Nonprofits only

7. pGL3 3 kb prom. CD274

   Plasmid

   #107004

   Purpose

   Modified Luciferase expression vector

   Depositor

   Julian Downward

   Article

   Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

   Insert

   CD274 (CD274 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAA3.stelN

   Plasmid

   #112011

   Purpose

   Expresses TelN enzyme

   Depositor

   Stephen Harrison

   Article

   Jenni et al Science. 2018 May 4;360(6388):552-558. d

   Insert

   TelN
   

   Use

   Tags

   strep and his

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   July 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. mApple-M1 Spastin

   Plasmid

   #134461

   Purpose

   M1 Spastin expression

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Chang et al J Cell Biol. 2019 Aug 5;218(8):2583-2599

   Insert

   M1 Spastin (SPAST Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Met to Ala change at amino acid 87

   Promoter

   CMV

   Available Since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.2-hBIN1-V5

    Plasmid

    #27303

    Depositor

    Robin Shaw

    Article

    Hong et al PLoS Biol. 2010 Feb . 8(2):e1000312.

    Insert

    bridging integrator 1 (BIN1), transcript variant 8 (BIN1 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pBAD_FLAG-GFP(Y39TAG)-6His

    Plasmid

    #223510

    Purpose

    Fluorescent reporter plasmid for genetic code expansion which consists of a modified GFP with a Amber stop codon on position Y39

    Depositor

    Edward Lemke

    Article

    Milles et al J Am Chem Soc. 2012 Mar 21;134(11):5187-

    Insert

    GFP(Y39TAG)
    

    Use

    Tags

    6xHis and FLAG

    Expression

    Bacterial

    Mutation

    Y39TAG

    Promoter

    Available Since

    Oct. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. ABE8.20-SpRY

    Plasmid

    #226859

    Purpose

    Expresses ABE8.20-SpRY base editor in mammalian cells with eGFP marker

    Depositor

    Alexis Komor

    Article

    Osgood et al Methods in Enzymology, February 5, 2025

    Insert

    ABE8.20-SpRY
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. EF1a_mCherry_P2A_Hygro_Barcode

    Plasmid

    #120426

    Purpose

    Barcoded lentiviral vector to express mCherry in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pWM_12x601_15bpLinker

    Plasmid

    #157786

    Purpose

    EcoRV digestion produces 12x601 chromatin assembly construct with 15 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    12x601_15bp linker
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGL3 2 kb prom. CD274

    Plasmid

    #107003

    Purpose

    Modified Luciferase expression vector

    Depositor

    Julian Downward

    Article

    Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

    Insert

    CD274 (CD274 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pWM_12x601_30bpLinker

    Plasmid

    #157789

    Purpose

    EcoRV digestion produces 12x601 chromatin assembly construct with 30 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    12x601_30bp linker
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. EB3-mNeonGreen

    Plasmid

    #191761

    Purpose

    Expression of mNeonGreen fused to EB3

    Depositor

    Daniel Gerlich

    Article

    Schneider et al Nature. 2022 Sep;609(7925):183-190. doi:

    Insert

    EB3-mNeonGreen (MAPRE3 Human)
    

    Use

    Tags

    mNeonGreen

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. Integrin β4-GFP

    Plasmid

    #205092

    Purpose

    Express GFP-tagged human integrin β4 in mammalian cells

    Depositor

    Bianxiao Cui

    Article

    Zhang et al Nat Cell Biol. 2023 Sep 28. doi: 10.1038

    Insert

    Integrin beta 4 (ITGB4 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. dead Sa Cas9

    Plasmid

    #138162

    Purpose

    For orthogonal R loop assay (use with Sp-derived base editor, Sp gRNA, and Sa gRNA for assay)

    Depositor

    David Liu

    Article

    Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

    Insert

    dSaCas9
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    D10A + N580A

    Promoter

    CMV

    Available Since

    Feb. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJAT17

    Plasmid

    #204298

    Purpose

    For introducing Cas9 under D. melanogaster nanos promoter. Markers with EYFP expressed in eyes.

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Insert

    3XP3-EYFP
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    3XP3

    Available Since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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