-
Plasmid#144859PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertNFKBID (NFKBID Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJOG1368
Plasmid#167301PurposeLevel 0 module (CDS1) for further assemblies based on the Modular Cloning system. FCY-UPP gene conferring sensitivity to 5-FluorocytosineDepositorInsertFCY-UPP fusion gene
UseSynthetic Biology; Moclo level 0 vectorTagsExpressionMutationPromoterAvailable sinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLEX_307
Plasmid#41392PurposeConstitutive lentiviral expression, SV40-puro; EF1a-gateway-V5 tagDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral; Gateway destination vectorTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceNov. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
TFORF0662
Plasmid#143063PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertCHD6 (CHD6 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
FRP792_insul-(lexA-box)2-PminCYC1-Citrine-TCYC1
Plasmid#58433PurposeInduces Citrine expression from a promoter containing 2 lexA boxes in yeast cellsDepositorInsertCitrineA206K
UseTagsExpressionYeastMutationPromoterinsul-(lexA-box)2-PminCYC1Available sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pYES2-HTH-HHT2
Plasmid#86482Purposeyeast expression of HHT2 with His10-TEV-HA tagDepositorInsertHHT2 (HHT2 Budding Yeast)
UseTagsHIS10-TEV-HA (HTH)ExpressionYeastMutationPromoterGAL1Available sinceJan. 27, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC19/mChe-Cs
Plasmid#183171PurposeConstruct for transient expression of cytoskeleton-targeted mCherry in plants.DepositorInsertmCherry-Cs
UseTags363 aa of A. thaliana FIMBRIN 1 (AtFIM1)ExpressionPlantMutationPromoterAvailable sinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJOG1336
Plasmid#167303PurposeLevel 0 module (3U+Ter) for further assemblies based on the Modular Cloning system. 3'UTR and flanking sequence of a Solanum lycopersicum Oleosin gene, arbitrarily called OLE2 (Sollyc03g112440)DepositorInsert3'UTR and Terminator SlOLE2
UseSynthetic Biology; Moclo level 0 vectorTagsExpressionMutationPromoterAvailable sinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJOG1334
Plasmid#167302PurposeLevel 0 module (Pro+5U) for further assemblies based on the Modular Cloning system. Promoter and 5'UTR of a Solanum lycopersicum Oleosin gene, arbitrarily called OLE2 (Sollyc03g112440)DepositorInsertPromoter SlOLE2
UseSynthetic Biology; Moclo level 0 vectorTagsExpressionMutationPromoterAvailable sinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPOTv7-blast-10Myc
Plasmid#181939PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsert10xMyc
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBabepuro-IL8
Plasmid#189608PurposeRetroviral expression vector for IL8DepositorInsertIL8 (CXCL8 Human)
UseRetroviralTagsExpressionMutationPromoterSV40 early promoterAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC19/mChe-Golgi
Plasmid#183169PurposeConstruct for transient expression of Golgi apparatus-targeted mCherry in plants.DepositorInsertmCherry-Golgi
UseTagsAtRER1B, A. thaliana endoplasmic reticulum retrie…ExpressionPlantMutationPromoterAvailable sinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFastBac HT JS-Munc18c
Plasmid#135555PurposeExpress mouse Munc18c in Sf9 cells, the resulted plasmid encodes an N-terminally His6-tagged Munc18c protein with a tobacco etch virus (TEV) cleavage site between the His6 tag and Munc18c.DepositorInsertMunc18c
UseTags6xHis tag and TEV siteExpressionInsectMutationPromoterpolyhedrin promoterAvailable sinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC19-link-sfGFP-FLAG:Kan
Plasmid#211833PurposePlasmid template that encodes the fluorescent protein sfGFP with a FLAG tag next to kanamycin resistance gene. This template was used to copy out sfGFP-FLAG-Kan for genetic tagging.DepositorInsertsfGFP-FLAG-Kan
UseTagsExpressionBacterialMutationNAPromoterAvailable sinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIVT/CamKII gamma CA
Plasmid#122142PurposeFor making constitutively active CamKII gamma RNADepositorInsertconstitutively active CamKII gamma (Camk2g Mouse)
UseFor in vitro transcriptionTagsExpressionMutationFirst 291 amino acid residues of CaMKII gammaPromoterT7Available sinceMarch 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CBA.loxP.ArcLightD.2A.nlsmCherry.loxP.WPRE.SV40
Plasmid#100030PurposeAAV expression of ArcLightDDepositorInsertArcLightD
UseAAVTags2A-mCherryExpressionMammalianMutationPromoterCBAAvailable sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19/CF-mChe
Plasmid#183166PurposeConstruct for transient expression of cytoplasmic foci-targeted mCherry in plants.DepositorInsertCF-mCherry
UseTagsAtTZF1, A. thaliana TANDEM ZINC FINGER PROTEIN 1ExpressionPlantMutationPromoterAvailable sinceJuly 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC19/Tp-mChe
Plasmid#183165PurposeConstruct for transient expression of tonoplast-targeted mCherry in plants.DepositorInsertTp-mCherry
UseTags28 aa of A. thaliana CALCINEURIN B-LIKE PROTEIN 2…ExpressionPlantMutationPromoterAvailable sinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQC V5 MCS IRES Puro
Plasmid#110342Purposeγ-Retroviral transfer vector for cloning and expressing your gene of interest. V5 N-Terminal tag, IRES-driven Puromycin selection.DepositorTypeEmpty backboneUseRetroviralTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
R777-E160 Hs.PDPK1-nostop
Plasmid#70444PurposeGateway ORF clone of human PDPK1 [NM_002613.4] without stop codon (for C-terminal fusions)DepositorInsertPDPK1 (PDPK1 Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only