163,606 results

1. pDGB3_alpha1-pAtUBQ10:HA-LRR-NEL-mcherry-Tnos

   Plasmid

   #211840

   Purpose

   Plant transient expression cassette for the catalytically inactive E3-DART ligase under a UBQ10 promoter.

   Depositor

   Marcela Rojas-Pierce

   Article

   Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

   Insert

   LRR-NEL
   

   Use

   Synthetic Biology

   Tags

   HA and mCherry

   Expression

   Plant

   Mutation

   Promoter

   UBIQUITIN10

   Available Since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pECIA14

   Plasmid

   #47051

   Purpose

   This is a Gateway destination vector for secreted expression in Drosophila cell culture by CuSO4 induction. Inserts will be pentamerized and tagged with Alkaline phosphatase, FLAG and His tags

   Depositor

   Chris Garcia

   Article

   Ozkan et al Cell. 2013 Jul 3;154(1):228-39. doi: 10.

   Type

   Empty backbone

   Use

   Secreted expression in drosophila culture

   Tags

   Alkaline Phosphatase (human, placental), Flag, HR…

   Expression

   Insect

   Mutation

   Promoter

   Metallothionein (Copper-Inducible)

   Available Since

   Aug. 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. EFS-SpdCas9

   Plasmid

   #210703

   Purpose

   This plasmid express EFS promoter driven human codon optimized SpdCas9

   Depositor

   Isaac Hilton

   Article

   Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

   Insert

   S. Pyogenes dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   D10A/H840A

   Promoter

   EF1a core promoter

   Available Since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPGK-T7/2-CD44v6 (fl)

   Plasmid

   #137816

   Purpose

   expression of CD44 proteins in mammalian cells

   Depositor

   Ursula Gunthert

   Article

   Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

   Insert

   CD44v6 (fl) (CD44 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   R296K in the V6 region [Please see depositor comm…

   Promoter

   PGK

   Available Since

   June 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJR022

   Plasmid

   #198820

   Purpose

   C. elegans optimized version of mScarlet

   Depositor

   Paul Sternberg

   Article

   Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

   Insert

   15xUAS::wrmScarlet::let-858 3'UTR
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available Since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCF140_Gag-SpyCas9-NLS

   Plasmid

   #225959

   Purpose

   CMV-Intron-Gag-SpyCas9-NLS. Expresses Gag-SpyCas9-NLS for VLP production.

   Depositor

   Christof Fellmann

   Article

   Ramadoss et al bioRxiv [Preprint]. 2024 Jun 26:2024.06.

   Insert

   CMV-Intron-Gag-SpyCas9-NLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDEST-FRT/TO-3xFLAG-REV7

   Plasmid

   #114127

   Purpose

   Expresses N-terminally tagged 3x FLAG-REV7 in mammalian cells

   Depositor

   Daniel Durocher

   Article

   Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

   Insert

   REV7 (MAD2L2 Human)
   

   Use

   Genomic integration, tetracycline inducible

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/TetO2

   Available Since

   Aug. 16, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pTH761-CEN-mCherry_v4

   Plasmid

   #40599

   Depositor

   Tobias von der Haar

   Article

   Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

   Insert

   mCherry (Red Fluorescent Protein variant) with yeast promoter and terminator sequences
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   mCherry ORF partially codon optimised for yeast e…

   Promoter

   GPD (TDH3)

   Available Since

   Oct. 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. FCK-Arch-GFP

   Plasmid

   #22217

   Purpose

   Lentiviral expression of Arch-GFP for optogenetic inhibition

   Depositor

   Edward Boyden

   Article

   Chow et al Nature. 2010 Jan 7. 463(7277):98-102.

   Insert

   Arch
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CaMKIIa

   Available Since

   Jan. 6, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. pECIA2

    Plasmid

    #47032

    Purpose

    This is a Gateway destination vector for secreted expression in Drosophila cell culture by induction via CuSO4. Inserts will be C-terminally tagged with Fc, V5 and hexahistidine tags.

    Depositor

    Chris Garcia

    Article

    Ozkan et al Cell. 2013 Jul 3;154(1):228-39. doi: 10.

    Type

    Empty backbone

    Use

    Secreted expression in drosophila culture

    Tags

    Fc(human IgG1), HRV 3C Protease Cleavage site, He…

    Expression

    Insect

    Mutation

    Promoter

    Metallothionein (Copper-Inducible)

    Available Since

    Aug. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-GFAP-iGluSnFR4s-PDGFR-WPRE

    Plasmid

    #234445

    Purpose

    AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.

    Depositor

    GENIE Project

    Article

    Aggarwal et al bioRxiv 2025.03.20.643984

    Insert

    iGluSnFR4s-PDGFR
    

    Use

    AAV

    Tags

    IgK chain and Myc epi tag-PDGFR TM Domain

    Expression

    Mammalian

    Mutation

    Promoter

    GFAP

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. UAS-3xHalo7::CAAX

    Plasmid

    #87646

    Purpose

    For creating drosophila transgenics expressing UAS-3xHalo7::CAAX

    Depositor

    Gregory Jefferis

    Article

    Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

    Insert

    3xHalo7::CAAX
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. Ai66(RCRL-tdT) targeting vector

    Plasmid

    #61578

    Purpose

    Target a Dre and Cre-dependent tdTomato expression cassette to the mouse Rosa26 locus

    Depositor

    Hongkui Zeng

    Article

    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

    Insert

    CAG-RSR-LSL-tdTomato
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available Since

    July 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRK5-mEGFP-HMGB1-WT-IDR

    Plasmid

    #194549

    Purpose

    Mammalian Expression of mEGFP-HMGB1 WT IDR Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

    Insert

    HMGB1 (HMGB1 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-GFAP-iGluSnFR4f-PDGFR-WPRE

    Plasmid

    #234446

    Purpose

    AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.

    Depositor

    GENIE Project

    Article

    Aggarwal et al bioRxiv 2025.03.20.643984

    Insert

    iGluSnFR4f-PDGFR
    

    Use

    AAV

    Tags

    IgK chain and Myc epi tag-PDGFR TM Domain

    Expression

    Mammalian

    Mutation

    Promoter

    GFAP

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRDB_054

    Plasmid

    #216096

    Purpose

    All-in-one, knockout, delivers Cas12a & gRNA

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Insert

    Cas12a [EnAs]
    

    Use

    CRISPR and Lentiviral; Assembled vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKLV2-U6(gCD44v2)-EF1a-BFP-Puro-Cas9(FZ)

    Plasmid

    #117134

    Purpose

    All-in-one expression vector for Cas9 and gRNA against CD44

    Depositor

    Kosuke Yusa

    Insert

    gCD44 v2 (Cd44 Synthetic)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Deleted BbsI site within WPRE, modified BFP

    Promoter

    human U6 promoter, human EF1a promoter

    Available Since

    April 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLXI_TRC403 SMARCB1 var 2

    Plasmid

    #111185

    Purpose

    Inducible vector (V5 tagged) with SMARCB1 variant 2

    Depositor

    William Hahn

    Article

    Hong et al Elife. 2019 Mar 12;8. pii: 44161. doi: 1

    Insert

    BAF47 (SMARCB1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Promoter

    TRE

    Available Since

    March 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-sL7-Cre-HA-WPRE

    Plasmid

    #204488

    Purpose

    For production of driver AAV virus for Purkinje cell-specific expression

    Depositor

    Samuel Wang

    Article

    Chen et al Nature. 2022 May;605(7911):722-727. doi:

    Insert

    Cre
    

    Use

    AAV, Cre/Lox, and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    sL7

    Available Since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. plv-AcGFP-SOD1 (wt)

    Plasmid

    #27138

    Depositor

    Elizabeth Fisher

    Article

    Stevens et al PLoS One. 2010 . 5(3):e9541.

    Insert

    SOD1 (SOD1 Human)
    

    Use

    Lentiviral

    Tags

    AcGFP

    Expression

    Mutation

    Promoter

    Available Since

    March 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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21. pLX_TRC311 ETV1

    Plasmid

    #74981

    Purpose

    Constitutive ETV1 ORF, blasticidin selection

    Depositor

    William Hahn

    Article

    Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

    Insert

    ETV1 (ETV1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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22. pLVTHM-mSox2-3xflag

    Plasmid

    #206376

    Purpose

    Expresses 3xflag fused mouse SOX2 in mammalian cells, for lentivirus generation.

    Depositor

    Ralf Jauch

    Article

    Hu et al Nucleic Acids Res. 2023 Aug 23:gkad597.

    Insert

    Sox2 (Sox2 Mouse)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. 3xFLAG-dCas9/pMXs-neo

    Plasmid

    #51260

    Purpose

    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

    Depositor

    Hodaka Fujii

    Article

    Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:

    Insert

    3xFLAG-dCas9
    

    Use

    CRISPR and Retroviral

    Tags

    3xFLAG tag and NLS (nuclear localization signal)

    Expression

    Mammalian

    Mutation

    human codon-optimized, D10A + H840A

    Promoter

    LTR

    Available Since

    Feb. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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24. xCas9

    Plasmid

    #138565

    Purpose

    Expresses human codon-optimized expanded PAM SpCas9 variant and blasticidin resistance: EFS promoter-xCas9-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

    Insert

    xCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    A262T, R324L, S409I, E480K, E543D, M694I, E1219V

    Promoter

    EFS

    Available Since

    June 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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25. pSKI

    Plasmid

    #232484

    Purpose

    pSKI can be used for single-copy transgene expression within the SKI PLACE System or for extrachromosomal arrays. It contains an MCS with 47 single cuts and two synthetic 900 bp homology arms.

    Depositor

    Carlos Silva García

    Article

    Dinneen et al G3 (Bethesda). 2025 Sep 19:jkaf220. doi:

    Insert

    pSKI
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    March 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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26. pENTR-SYNZIP17

    Plasmid

    #80671

    Purpose

    Encodes the synthetic coiled coil peptide SYNZIP17

    Depositor

    Amy Keating

    Article

    Thompson et al ACS Synth Biol. 2012 Apr 20;1(4):118-29.

    Insert

    SYNZIP17
    

    Use

    Entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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27. pUC19 PKC delta

    Plasmid

    #8427

    Depositor

    Frederic Mushinski

    Article

    Mischak et al Biochemistry 1991 Aug 13;30(32):7925-31.

    Insert

    Mouse Protein Kinase C delta (Prkcd Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 28, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pMEXC3H

    Plasmid

    #110080

    Purpose

    Analogous to pMINTC3H, but containing oriM for episomal replication in mycobacteria.

    Depositor

    Markus Seeger

    Article

    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    3C cleavage site - His10 Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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29. pFLAG_OriM

    Plasmid

    #110097

    Purpose

    Analogous to pFLAG_attP, but containing oriM instead of attP, thus being a replicative vector.

    Depositor

    Markus Seeger

    Article

    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    3xFLAG

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. GFP-Trak1

    Plasmid

    #127621

    Purpose

    N-terminal GFP-tagged mouse Trak1

    Depositor

    Josef Kittler

    Article

    Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

    Insert

    Trak1 (Trak1 Mouse)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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