We narrowed to 4,840 results for: phy

1. pS24·Pm-GFP

   Plasmid

   #122594

   Purpose

   Expresses GFP under the regulatory control of Pm. Expression needs additional xyls gene

   Depositor

   Pablo Ivan Nikel

   Article

   Volke et al Microb Biotechnol. 2019 Mar 12. doi: 10.

   Insert

   Pm promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Pm

   Available since

   March 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. TR therapy reporter

   Plasmid

   #179083

   Purpose

   Fluorescent reporter for alternative splicing

   Depositor

   Thurman Wheeler

   Article

   Hu et al Nat Commun. 2018 Dec 7;9(1):5227. doi: 1

   Inserts

   DsRed1
   EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. LZF43: Cre-on CAG-CheRiff-CFP (DIO)

   Plasmid

   #104114

   Purpose

   synOptopatch construct

   Depositor

   Adam Cohen

   Article

   Fan et al Nat Methods. 2018 Oct;15(10):823-831. do

   Insert

   CheRiff-CFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGAPi

   Plasmid

   #127547

   Purpose

   Gateway-based RNAi vector to clone target gene for silencing in tandem with APT transcript segment

   Depositor

   Luis Vidali

   Article

   Orr et al Plant Physiol. 2020 Aug 6. pii: pp.20.00

   Type

   Empty backbone

   Use

   RNAi

   Tags

   Expression

   Mutation

   Promoter

   Ubiquitin

   Available since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pUG-248

   Plasmid

   #104709

   Purpose

   Cloning Module

   Depositor

   Marco Trujillo

   Article

   Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

   Insert

   PUB22 C13A (PUB22 Mustard Weed)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   C13A, BpiI/BsaI sites removed by point-mutation

   Promoter

   Available since

   Feb. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pUG-21

   Plasmid

   #104707

   Purpose

   Cloning Module

   Depositor

   Marco Trujillo

   Article

   Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

   Insert

   PUB22 WT (PUB22 Mustard Weed)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BpiI/BsaI sites removed by point-mutation

   Promoter

   Available since

   Feb. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pUG-UBC1

   Plasmid

   #104666

   Purpose

   Cloning Module

   Depositor

   Marco Trujillo

   Article

   Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

   Insert

   AtUBC1 (UBC1 Mustard Weed)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pTH1-SpSrtA

   Plasmid

   #64987

   Purpose

   Expresses the wild type of Streptococcus pyogenes sortase A in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

   Insert

   S. pyogenes sortase A
   

   Use

   Tags

   His6 tag, Mxe GyrA intein, and chitin-binding pro…

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-81

   Promoter

   T7 promoter

   Available since

   June 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pQIq_MRGS_HIS8_(27F3)_FLAG

   Plasmid

   #199615

   Purpose

   Encodes DARPin-FLAG 27F3 for bacterial expression and column purification

   Depositor

   Shiva Tyagarajan

   Article

   Campbell et al Elife. 2022 Oct 31;11:e80895. doi: 10.75

   Insert

   DARPin-FLAG 27F3
   

   Use

   Tags

   8xHis and FLAG

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available since

   May 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pIntegrase_12

    Plasmid

    #60583

    Purpose

    plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

    Depositor

    Christopher Voigt

    Article

    Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

    Insert

    Integrase_12
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pBAD

    Available since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pB_35S/mEGFP

    Plasmid

    #135320

    Purpose

    mEGFP expression regulated by the CaMV35S promoter, co-expressed BASTA resistance selectable marker (bar)

    Depositor

    Mathias Pribil

    Article

    Behrendorff et al Physiol Plant. 2020 Feb 25. doi: 10.1111

    Insert

    mEGFP
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Cauliflower mosaic virus 35S

    Available since

    Jan. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. ATM N-terminal sgRNA

    Plasmid

    #207089

    Purpose

    pX330 based plasmid for expression of Cas9 and the ATCATTAAGTACTAGACTCA sgRNA to target the ATM locus.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    ATCATTAAGTACTAGACTCA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    April 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. MAC_C_STYK1

    Plasmid

    #187792

    Purpose

    MAC-tagged gene expression of human STYK1

    Depositor

    Markku Varjosalo

    Article

    Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

    Insert

    STYK1 (STYK1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pZac2.1 gfaABC1D-Cyto-GCaMP3

    Plasmid

    #44331

    Depositor

    Baljit Khakh

    Article

    Shigetomi et al J Gen Physiol. 2013 Apr 15.

    Insert

    GCaMP3
    

    Use

    AAV; Adeno-associated virus ()

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pENTR-D-TOPO-kcnn2

    Plasmid

    #164958

    Purpose

    Zebrafish kcnn2 gene CDS

    Depositor

    GuangJun Zhang

    Article

    Silic et al Dev Dyn. 2021 Mar 16. doi: 10.1002/dvdy.

    Insert

    kcnn2 (kcnn2 Zebrafish)
    

    Use

    Pcr cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEPOR0SP0020

    Plasmid

    #117531

    Purpose

    Level0 Golden Gate part: CDS, SaCas9

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    SaCas9 (with stop codon)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pQIq_MRGS_HIS8_(27G2)_FLAG

    Plasmid

    #199616

    Purpose

    Encodes DARPin-FLAG 27G2 for bacterial expression and column purification

    Depositor

    Shiva Tyagarajan

    Article

    Campbell et al Elife. 2022 Oct 31;11:e80895. doi: 10.75

    Insert

    DARPin-FLAG 27G2
    

    Use

    Tags

    8xHis and FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    May 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCfB8379

    Plasmid

    #126913

    Purpose

    Plasmid containing integration cassette at X-4 integration site in Saccharomyces cerevisiae for overexpression of XdCrtI and XdCrtYB

    Depositor

    Irina Borodina

    Article

    Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

    Insert

    XdCrtI and XdCrtYB
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCfB8381

    Plasmid

    #126915

    Purpose

    Plasmid containing integration cassette at XI-3 integration site in Saccharomyces cerevisiae for overexpression of XdCrtI and tHMG1

    Depositor

    Irina Borodina

    Article

    Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

    Insert

    XdCrtI and tHMG1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p3991 pET ubcH9

    Plasmid

    #8651

    Depositor

    Peter Howley

    Article

    Kumar et al J Biol Chem 1997 May 23;272(21):13548-54

    Insert

    UbcH9 (UBE2I Human)
    

    Use

    Tags

    HA

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

21. pT2/SVNeo-103

    Plasmid

    #79841

    Purpose

    Expresses RpBphP1-mCherry-TetR under CMVd1 promoter

    Depositor

    Vladislav Verkhusha

    Article

    Kaberniuk et al Nat Methods. 2016 May 9. doi: 10.1038/nm

    Insert

    RpBphP1-mCherry-Intersectin1 DHPH domain
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMVd1

    Available since

    July 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pRVdG-mCherry-N-P-M-EGFP-SynPhRFP-L

    Plasmid

    #52481

    Purpose

    Expresses mCherry, EGFP and Synaptophysin-TagRFP-T

    Depositor

    Sebastian Seung

    Article

    Wickersham et al Nat Commun. 2013;4:2332. doi: 10.1038/nc

    Inserts

    mCherry
    EGFP
    Synaptophysin-TagRFP-T
    

    Use

    Rabies virus

    Tags

    TagRFP-T

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 23, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pMOS017E: Entry vector for: ratiometric pHluorin (cytosolic)

    Plasmid

    #163069

    Purpose

    Entry vector for: ratiometric pHluorin (cytosolic)

    Depositor

    Adam Cohen

    Article

    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

    Insert

    ratiometric pHluorin
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pC0.053

    Plasmid

    #119571

    Purpose

    Level 0 part. Promoter

    Depositor

    Alistair McCormick

    Article

    Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

    Insert

    PnirA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    RBS* added (22 bp) upstream of ATG.

    Promoter

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pBAD-O-GECO1

    Plasmid

    #113938

    Purpose

    Bacterial expression of orange fluorescent Ca2+ sensor O-GECO1

    Depositor

    Thomas Hughes, Robert Campbell

    Article

    Molina et al Biophys J. 2019 May 21;116(10):1873-1886

    Insert

    O-GECO1
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCS2 xRor2-mCherry

    Plasmid

    #67162

    Purpose

    mammalian expression / in vitro transcription

    Depositor

    Alexandra Schambony

    Article

    Feike et al Biochem Biophys Res Commun. 2010 Oct 1;4

    Insert

    Tyrosine-protein kinase transmembrane receptor ROR2 (ror2 Frog)
    

    Use

    In vitro transcription

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 16, 2015

    Availability

    Academic Institutions and Nonprofits only

27. pZac2.1 gfaABC1D-Lck-GCaMP3

    Plasmid

    #44330

    Depositor

    Baljit Khakh

    Article

    Shigetomi et al J Gen Physiol. 2013 Apr 15.

    Insert

    Lck-GCaMP3
    

    Use

    AAV; Adeno-associated virus ()

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Mammalian_SaAID

    Plasmid

    #188653

    Purpose

    Expresses SaAID in mammalian cells

    Depositor

    Keiji Nishida

    Article

    Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:

    Insert

    nSaCas9-AID-UGI
    

    Use

    Tags

    UGI

    Expression

    Mammalian

    Mutation

    D10A for SaCas9

    Promoter

    pCMV

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCBC-DT1T2.2

    Plasmid

    #134752

    Purpose

    PCR template for sgRNA cloning

    Depositor

    Qi-Jun Chen

    Article

    Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

    Insert

    sgRNA-AtU6_29p
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pGB411

    Plasmid

    #134760

    Purpose

    pCambia CRISPR/Cas9

    Depositor

    Qi-Jun Chen

    Article

    Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

    Insert

    CRISPR/Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCBC-MT1T2.2

    Plasmid

    #134751

    Purpose

    PCR template for sgRNA cloning

    Depositor

    Qi-Jun Chen

    Article

    Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

    Insert

    sgRNA-TaU3p
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. VV246: CoChR(C108S)-GCaMP6f-ER in fck

    Plasmid

    #58521

    Purpose

    Expresses CoChR(C108S) fused to GCaMP6f in mammalian cells

    Depositor

    Adam Cohen

    Article

    Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

    Insert

    CoChR(C108S)-GCaMP6f-ER
    

    Use

    Lentiviral

    Tags

    GCaMP6f

    Expression

    Mammalian

    Mutation

    changed Cysteine 108 to Serine

    Promoter

    a-CamKII

    Available since

    Nov. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pYPQ155

    Plasmid

    #69306

    Purpose

    Gateway entry vector with AteCas9D10A

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Insert

    AteCas9D10A (Arabidopsis codon optimized)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A mutation; NLS fusion

    Promoter

    Available since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. IFP1.4-N1

    Plasmid

    #54783

    Purpose

    Localization: N1 Cloning Vector, Excitation: 684, Emission: 708

    Depositor

    Michael Davidson, Roger Tsien

    Article

    Shu et al Science. 2009 May 8;324(5928):804-7. doi

    Type

    Empty backbone

    Use

    Tags

    IFP1.4

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 20, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

35. pSW549 YFP/mVenus

    Plasmid

    #115984

    Purpose

    entry vector for GreenGate cloning method, CDS

    Depositor

    Jan Lohmann

    Article

    Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

    Insert

    mVenus
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pRSETA_crE6G2

    Plasmid

    #172929

    Purpose

    Crowding FRET sensor with mCerulean3 and mCitrine for E. coli expression. Linker comprises 2 helices, and a 6AA GSG domain

    Depositor

    Arnold Boersma

    Article

    Liu et al Biophys J. 2017 May 9;112(9):1929-1939.

    Insert

    CrowdingSensorE6G2
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Val317Gly, Met316Gly

    Promoter

    T7

    Available since

    Aug. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. mEos2-Tubulin-C-18

    Plasmid

    #57432

    Purpose

    Localization: Microtubules, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

    Insert

    Tubulin (TUBA1B Human)
    

    Use

    Tags

    mEos2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

38. pACYC_HA-ZmSAE1_ZmSAE2-FLAG

    Plasmid

    #91944

    Purpose

    Bacterial expression of maize SAE1 and SAE2

    Depositor

    Richard Vierstra

    Article

    Augustine et al Plant Physiol. 2016 Jul;171(3):2191-210.

    Inserts

    HA-ZmSAE1
    ZmSAE2-FLAG
    

    Use

    Tags

    FLAG and HA

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pFA-GFP

    Plasmid

    #133009

    Purpose

    pFA carrying a sfGFP gene

    Depositor

    David Savage

    Article

    Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

    Insert

    sfGFP
    

    Use

    Tags

    6xhis

    Expression

    Bacterial

    Mutation

    Promoter

    tetR

    Available since

    May 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pTn7·xylS

    Plasmid

    #122591

    Purpose

    Plasmid for genomic integraction of the xylS regulator into the Tn7 insertion site

    Depositor

    Pablo Ivan Nikel

    Article

    Volke et al Microb Biotechnol. 2019 Mar 12. doi: 10.

    Insert

    xylS
    

    Use

    Synthetic Biology; Tn7 genomic integration

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pm

    Available since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge