Addgene

We narrowed to 9,510 results for: Gnas

Showing: 2251 - 2300 of 9510 results

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pQE60 Trx1 CSAAA

Plasmid

#98401

Purpose

Bacterial expression vector for Trx1 CSAAA

Depositor

Tobias Dick

Article

Schwertassek et al EMBO J. 2007 Jul 11;26(13):3086-97. Epub

Insert

Trx1 CSAAA (TXN Human)

Use

Tags

Expression

Bacterial

Mutation

C35S, C62A, C69A, C73A

Promoter

Available since

July 31, 2017

Availability

Academic Institutions and Nonprofits only

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pBabe puro RalB wt

Plasmid

#19720

Depositor

Channing Der

Article

Lim et al Curr Biol. 2006 Dec 19. 16(24):2385-94.

Insert

V-ral simian leukemia viral oncogene homolog B (RALB Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only
pJH2972

Plasmid

#175285

Purpose

Pmyo-3 GFP::DAF-16a unc-54 3' UTR C.elegans muscle expression of isoforms a of DAF-16

Depositor

Mei Zhen

Article

Hung et al Development. 2014 Apr;141(8):1767-79. do

Insert

DAF-16a (daf-16 Nematode)

Use

Tags

GFP

Expression

Worm

Mutation

Promoter

Pmyo-3

Available since

Nov. 10, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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NbALFA-TEV219

Plasmid

#201487

Purpose

TANGO detection of V5-tagged protein

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

NbALFA-TEV219

Use

Tags

TEV219

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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NFkBm2

Plasmid

#15090

Depositor

David Baltimore

Article

Taganov et al Proc Natl Acad Sci U S A. 2006 Aug 15. 1

Insert

miR-146a promoter

Use

Tags

Expression

Mammalian

Mutation

2 NFkB binding sites mutated

Promoter

Available since

June 6, 2007

Availability

Academic Institutions and Nonprofits only
pBAD/HisB (TIRSTD)-sfGFP

Plasmid

#189674

Purpose

titratable expression of sfGFP in bacteria

Depositor

Daniel Daley

Article

Shilling et al Synth Biol (Oxf). 2022 Jul 5;7(1):ysac00

Insert

sfGFP

Use

Tags

His-T7-EK

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pSLQ11434

Plasmid

#183625

Purpose

pHR-R(HRE-minCMV-BFP-Triplex-2X crGFP*ABE3-SV40pA)-EFS-Hygro-T2A-rtTA-WPRE

Depositor

Stanley Qi

Article

Kempton et al Nat Chem Biol. 2022 May 30. pii: 10.1038

Insert

Cas12 Guide (crGFP*)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pSLQ11435

Plasmid

#183626

Purpose

pHR-R(CRE-minCMV-BFP-Triplex-2X crGFP*ABE3-SV40pA)-EFS-Hygro-T2A-rtTA-WPRE

Depositor

Stanley Qi

Article

Kempton et al Nat Chem Biol. 2022 May 30. pii: 10.1038

Insert

Cas12 Guide (crGFP*)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pFlox_N-RPB1_BSD_FKBPF36V_EYFP

Plasmid

#163884

Purpose

Acute Depletion of RBP1-EYFP

Depositor

Cornelis Murre

Article

Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

Insert

Rbp1 (Polr2a Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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PLEXIN A4

Plasmid

#58239

Purpose

Riboprobe for in vitro transcription of Plexin A4

Depositor

Christiana Ruhrberg

Article

Schwarz et al Dev Biol. 2008 Dec 1;324(1):1-9. doi: 10

Insert

Plexin A4 (Plxna4 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only
Wnt9B STOP

Plasmid

#35882

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt9B (WNT9B Human)

Use

Entry vector

Tags

Expression

Mutation

SNP Met106-->Thr

Promoter

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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NbV5-TEV219

Plasmid

#201481

Purpose

TANGO detection of V5-tagged protein

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

NbV5-TEV219

Use

Tags

TEV219

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-mSNX27-ΔF3

Plasmid

#163618

Purpose

mouse SNX27 without the F3 domain cDNA C-terminally tagged with GFP

Depositor

Josef Kittler

Article

Halff et al Cell Rep. 2019 Nov 26;29(9):2599-2607.e6

Insert

mouse SNX27-ΔF3 (Snx27 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

deletion F3 domain

Promoter

Available since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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CS2 Flag-Smad3 (EPSM)

Plasmid

#14963

Depositor

Joan Massague

Article

Kretzschmar et al Genes Dev. 1999 Apr 1. 13(7):804-16.

Insert

Smad3 (EPSM) (SMAD3 Human)

Use

Xenopus/mammalian/avian/zebrafish

Tags

Flag

Expression

Mutation

T178V, S203A, S207A, S212A

Promoter

Available since

May 17, 2007

Availability

Academic Institutions and Nonprofits only
CMVTO-p450-hcvs-B2AD2-26Sfur-SEAP-26sFur-B2AD-tevs-KKMP

Plasmid

#179644

Purpose

AND gate (TEVP/HCVP) SEAP RELEASE construct

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

SEAP RELEASE

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pVITRO1-iRFP702-LaminB1

Plasmid

#184456

Purpose

Nuclear membrane-targeted iRFP702 expression in mammalian cells

Depositor

Chuan-Hsiang Huang

Article

Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

Insert

iRFP702-LaminB1

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

Rat EF1α

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pGC680

Plasmid

#107018

Purpose

GFP reporter of lag-2 driven by 3 kb promoter

Depositor

Jane Hubbard

Article

Pekar et al Development. 2017 Aug 15;144(16):2896-29

Insert

GFP-PH

Use

Tags

GFP

Expression

Worm

Mutation

Promoter

lag-2

Available since

Dec. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pAGM9121_pMOX

Plasmid

#160922

Purpose

Methanol oxidase (MOX) promoter module for episomal P. pastoris promoter shuffling

Depositor

Sylvestre Marillonnet

Article

Pullmann et al ACS Synth Biol. 2021 Jun 2. doi: 10.1021

Insert

Methanol oxidase promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pCI HA NEDD4L S382A

Plasmid

#27036

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

NEDD4L (NEDD4L Human)

Use

Tags

HA

Expression

Mammalian

Mutation

S382A

Promoter

Available since

Jan. 25, 2011

Availability

Academic Institutions and Nonprofits only
KG#65

Plasmid

#110933

Purpose

Expresses proteins in cholinergic neurons in the ventral nerve cord of C. elegans

Depositor

Kenneth Miller

Article

Charlie et al Genetics. 2006 Feb;172(2):943-61. doi: 1

Inserts

unc-17 beta promoter
unc-54 3' control region

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

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Rag2 T490A

Plasmid

#115650

Purpose

expression of full length Rag2 with T490A mutation

Depositor

Martin Gellert

Article

Grundy et al Proc Natl Acad Sci U S A. 2010 Dec 28;10

Insert

Rag2 (Rag2 Mouse)

Use

Tags

8XHis-MBP-PreScission

Expression

Mammalian

Mutation

T490A

Promoter

Available since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR-TIR1

Plasmid

#83875

Purpose

Gateway donor vector for TIR1

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

TIR1 (TIR1 Mustard Weed)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Oct. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pVITRO1-mCardinal-CAXX

Plasmid

#184451

Purpose

Plasma membrane-targeted mCardinal expression in mammalian cells

Depositor

Chuan-Hsiang Huang

Article

Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

Insert

mCardinal-CAAX

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

Rat EF1α

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pCI HA NEDD4L S468A

Plasmid

#27037

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

NEDD4L (NEDD4L Human)

Use

Tags

HA

Expression

Mammalian

Mutation

S468A

Promoter

Available since

Jan. 25, 2011

Availability

Academic Institutions and Nonprofits only
pEGFP-N1-mSNX27-H112A

Plasmid

#163619

Purpose

mouse SNX27 cDNA with a point mutation H112A C-terminally tagged with GFP

Depositor

Josef Kittler

Article

Halff et al Cell Rep. 2019 Nov 26;29(9):2599-2607.e6

Insert

mouse SNX27-H112A (Snx27 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

H112A

Promoter

Available since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX4T-1-mMcl-1

Plasmid

#25717

Depositor

Roger Davis

Article

Morel et al Mol Cell Biol. 2009 Jul . 29(14):3845-52

Insert

MCL1 (Mcl1 Mouse)

Use

Tags

Gst

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 20, 2010

Availability

Academic Institutions and Nonprofits only
pBAD/HisB (TIRSTD)-mNG

Plasmid

#189721

Purpose

titratable expression of mNeonGreen in bacteria

Depositor

Daniel Daley

Article

Shilling et al Synth Biol (Oxf). 2022 Jul 5;7(1):ysac00

Insert

mNeonGreen

Use

Tags

His-T7-EK

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSB1A3-AD009

Plasmid

#116850

Purpose

Expresses araC constitutively and alpha-hemolysin under a pBAD promoter; alternate name pSB1A2-AD009

Depositor

Friedrich Simmel

Article

Dupin et al Nat Chem. 2018 Nov 26. pii: 10.1038/s415

Inserts

AraC
alpha-HL

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
JGE301_pAAV-hSyn-DIO-Flag-hM3Dq-V2(2D)-ABIcs-mCherry

Plasmid

#107246

Purpose

hM3DREADD fused to ABIcs for abscisic acid mediated recruitment of PYLcs labelled proteins.

Depositor

Bryan Roth

Article

Roth et al bioRxiv 251769

Insert

hM3DREADD

Use

AAV

Tags

ABIcs, FLAG, and mCherry

Expression

Mutation

T to D and S to D mutations in V2-tail

Promoter

hSYN

Available since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
3XFlag-pLPC

Plasmid

#73560

Purpose

vector encoding 3 Flag tags

Depositor

Gerardo Ferbeyre

Article

Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

Insert

3X-Flag

Use

Retroviral

Tags

3X-FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only
pDHt-SK

Plasmid

#63615

Purpose

The multiple cloning site of pGreenII0000 cloned into binary vector pDHt

Depositor

Seogchan Kang

Article

Khang et al Fungal Genet Biol. 2005 Jun;42(6):483-92

Type

Empty backbone

Use

Fungal expression

Tags

Expression

Mutation

Promoter

Available since

July 27, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pVITRO1-TagRFP-NES

Plasmid

#184449

Purpose

Cytosolic TagRFP expression in mammalian cells

Depositor

Chuan-Hsiang Huang

Article

Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

Insert

TagRFP-NES

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

Rat EF1α

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV_hSyn_Ilmol

Plasmid

#120311

Purpose

Expresssion of Ilmol preferentially in neurons

Depositor

Bradley Baker

Article

Yi et al Sci Rep. 2018 Oct 12;8(1):15199. doi: 10

Insert

Ilmol

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pEF-hSEMA4C-Fc

Plasmid

#74449

Purpose

Expresses human Semaphorin-4C ectodomain fused to human Fc in mammalian cells

Depositor

Roland Friedel

Article

Le et al Oncotarget. 2015 Mar 30;6(9):7293-304.

Insert

human Semaphorin-4C ectodomain with human Fc tag (SEMA4C Human)

Use

Tags

human Fc tag

Expression

Mammalian

Mutation

Promoter

EF1-alpha

Available since

May 2, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pAGM9121_pFMD

Plasmid

#160921

Purpose

Formate dehydrogenase (FMD) promoter module for episomal P. pastoris promoter shuffling

Depositor

Sylvestre Marillonnet

Article

Pullmann et al ACS Synth Biol. 2021 Jun 2. doi: 10.1021

Insert

Formate dehydrogenase promoter

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI and BbsI domesticated

Promoter

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pISH-Galpha-i2

Plasmid

#15929

Depositor

Peter Mombaerts

Article

Rodriguez et al Nat Neurosci. 2002 Feb . 5(2):134-40.

Insert

Galpha-i2 (Gnai2 Mouse)

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only
pEBG-Pak1 83-149 L107F

Plasmid

#12215

Depositor

Jonathan Chernoff

Article

Xiao et al J Biol Chem. 2002 Jan 11. 277(2):883-6.

Insert

Pak1 (PAK1 Human)

Use

Tags

GST

Expression

Mammalian

Mutation

Autoinhibitory domain (aa 83-149) with L107F muta…

Promoter

Available since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only
pET28-SUMO-KaiB

Plasmid

#68050

Purpose

Strain: AM5116, Used for protein purification

Depositor

Susan Golden

Article

Kim et al Proc Natl Acad Sci U S A. 2012 Oct 30;10

Insert

KaiB

Use

Tags

6xHis and SUMO

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pTMt_NES_TCS(Q’G)_HA-dCas9(N)_P2A-Puro-WPRE

Plasmid

#101101

Purpose

Encodes dCas9(N) fused to transmembrane tether

Depositor

Tudor Fulga

Article

Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

Insert

TMt-dCas9(N)

Use

CRISPR and Synthetic Biology

Tags

HA and Myc

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
Wnt11 STOP

Plasmid

#35885

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

WNT11 (WNT11 Human)

Use

Entry vector

Tags

Expression

Mutation

None

Promoter

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP2-NbV5

Plasmid

#201478

Purpose

BRET2 detection of V5-tagged protein

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

GFP2-NbV5

Use

Tags

GFP2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Rluc8-NbV5

Plasmid

#201479

Purpose

BRET detection of V5-tagged protein

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

NbV5-Rluc8

Use

Tags

Rluc8

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGST TCF4 DBD

Plasmid

#16488

Depositor

Bert Vogelstein

Article

Morin et al Science. 1997 Mar 21. 275(5307):1787-90.

Insert

TCF4 DNA binding domain (TCF7L2 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

aa 265-496

Promoter

Available since

April 7, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA-Wnt3-V5

Plasmid

#35928

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt3 (WNT3 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

None

Promoter

CMV

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pARS3_MUbCAS9_MC

Plasmid

#128767

Purpose

T-DNA binary vector for CRISPR/Cas9 gene editing in rice; has a MCS for cloning gRNA

Depositor

Zachary Nimchuk

Article

Worthen et al Development. 2019 Jun 3. pii: dev.174870

Type

Empty backbone

Use

Tags

HA

Expression

Plant

Mutation

Promoter

Available since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pDRF1-GW AKAR3-EV-NR

Plasmid

#182534

Purpose

Expresses the non responsive PKA FRET sensor AKAR3-EV (T506A) in budding yeast

Depositor

Bas Teusink

Article

Botman et al FEMS Yeast Res. 2023 Jan 4;23:foad029. d

Insert

AKAR3-EV-NR

Use

Tags

Expression

Yeast

Mutation

T506A mutation

Promoter

PMA1

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC57-Kan_FR13_APikL2

Plasmid

#123648

Purpose

Level 0 Golden-Gate compatible vector

Depositor

Sophien Kamoun

Article

Petit-Houdenot et al Mol Plant Microbe Interact. 2020 Aug;33(

Insert

FR13_APikL2

Use

Golden gate

Tags

Expression

Mutation

Promoter

Available since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-Rnd2

Plasmid

#23228

Depositor

Channing Der

Article

Roberts et al J Biol Chem. 2008 Sep 12. 283(37):25150-

Insert

Rnd2 (RND2 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2010

Availability

Academic Institutions and Nonprofits only
pET28-SUMO-KaiA

Plasmid

#68049

Purpose

Strain: AM5115, Used for protein purification

Depositor

Susan Golden

Article

Kim et al Proc Natl Acad Sci U S A. 2012 Oct 30;10

Insert

KaiA

Use

Tags

6xHis and SUMO

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pGC630

Plasmid

#107013

Purpose

GFP reporter of lag-2 driven by 2 kb promoter

Depositor

Jane Hubbard

Article

Pekar et al Development. 2017 Aug 15;144(16):2896-29

Insert

GFP-PH

Use

Tags

Expression

Worm

Mutation

Promoter

lag-2

Available since

Dec. 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge

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