164,577 results

1. pCX-DI-2A

   Plasmid

   #74671

   Purpose

   Mammalian expression of DI-2A (hCD73/F2A/hCD39)

   Depositor

   Roberto Giovannoni

   Article

   De Giorgi et al Nucleosides Nucleotides Nucleic Acids. 2

   Inserts

   CD73 (NT5E Human)
   CD39 (ENTPD1 Human)
   

   Use

   Tags

   F2A

   Expression

   Mammalian

   Mutation

   Promoter

   pCAGGS

   Available Since

   Feb. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. TRE-UBC-hCD55-T2A-GFP

   Plasmid

   #205459

   Purpose

   lentiviral plasmid for expression of human CD55

   Depositor

   Deepta Bhattacharya

   Article

   Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

   Insert

   hCD55 (CD55 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   UBC

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. FLAG-VAMP8

   Plasmid

   #45912

   Depositor

   Noboru Mizushima

   Article

   Itakura et al Cell. 2012 Dec 7;151(6):1256-69. doi: 10

   Insert

   vesicle-associated membrane protein 8 (VAMP8 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Dec. 20, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pEB2-E2-Crimson

   Plasmid

   #104010

   Purpose

   Plasmid encoding E2-Crimson

   Depositor

   Philippe Cluzel

   Article

   Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

   Insert

   E2-Crimson
   

   Use

   Low copy

   Tags

   Expression

   Bacterial

   Mutation

   Mutations relative to DsRed (R2D, S4T, K5E, E10P,…

   Promoter

   proC

   Available Since

   Dec. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTRE-TIGHT-EGFP-donor fw copy

   Plasmid

   #22074

   Depositor

   Rudolf Jaenisch

   Article

   Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

   Insert

   TETO-eGFP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. AiP2048 - pAAV-AiE2543m-minBG-iCre(R297T)-BGHpA

   Plasmid

   #220727

   Purpose

   AiE2543m is an enhancer sequence, designed to induce cre-dependent recombination in specific populations of brain cells

   Depositor

   Bosiljka Tasic, Allen Institute for Brain Science

   Article

   Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

   Insert

   iCre(R297T)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   minBG

   Available Since

   March 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pWM_12x601_20bpLinker

   Plasmid

   #157787

   Purpose

   EcoRV digestion produces 12x601 chromatin assembly construct with 20 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

   Depositor

   Michael Rosen

   Article

   Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

   Insert

   12x601_20bp linker
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-CD40L-mgSrtA

   Plasmid

   #125795

   Purpose

   for co-expression of CD40L (CD40 ligand) and mgSrtA on cell membrane. For proof-of-concept monitoring and labeling of CD40 positive cells.

   Depositor

   P. Chen

   Article

   Ge et al J Am Chem Soc. 2019 Feb 6;141(5):1833-18

   Insert

   CD40L-mgSrtA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCBC-MT3T4

   Plasmid

   #50595

   Purpose

   CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites

   Depositor

   Qi-Jun Chen

   Article

   Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

   Insert

   T3, gRNA scaffold, U6-26t plus, OsU3p, T4
   

   Use

   CRISPR; Pcr template

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Dec. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA-mC/EBPb

    Plasmid

    #49198

    Purpose

    expression of mouse C/EBPbeta in mammalian cells

    Depositor

    Jed Friedman

    Insert

    C/EBPbeta (Cebpb Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. KIF1A(1-393)-GCN4-2xmCherry-EF(C)

    Plasmid

    #61665

    Purpose

    Tandem mCherry-labeled, forced-dimeric, truncated kinesin-3 fused to EF Hand linker

    Depositor

    Kristen Verhey

    Article

    Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

    Insert

    KIF1A(1-393)-GCN4-2xmCherry-EF(C) (KIF1A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. EF1a_MYC_P2A_Hygro_Barcode

    Plasmid

    #120461

    Purpose

    Barcoded lentiviral vector to express MYC in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    MYC (MYC Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA-SET-FLAG-HA

    Plasmid

    #24998

    Depositor

    Judy Lieberman

    Article

    Yan et al PLoS Pathog. 2009 Mar . 5(3):e1000327.

    Insert

    SET (SET Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDEST-CMV GFP-LC3B WT

    Plasmid

    #123114

    Purpose

    Expresses EGFP-LC3B wild-type in mammalian cells. Fluorescent reporter for autophagosomes.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. ancBE4-SpRY

    Plasmid

    #226855

    Purpose

    Expresses ancBE4max-SpRY base editor in mammalian cells with eGFP marker

    Depositor

    Alexis Komor

    Article

    Osgood et al Methods in Enzymology, February 5, 2025

    Insert

    ancBE4max-SpRY
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMRX-IZ-ANXA7-mRuby3

    Plasmid

    #184893

    Purpose

    Stable expression of ANXA7-mRuby3 (Annexin A7-mRuby3) in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Yim et al FEBS Lett. 2022 Mar 11. doi: 10.1002/187

    Insert

    ANXA7 (ANXA7 Human)
    

    Use

    Tags

    mRuby3

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSH-EFIRES-P-AtAFB2-mCherry

    Plasmid

    #129716

    Purpose

    AAVS1 safe harbor targeting vector expressing AtAFB2-mCherry

    Depositor

    Elina Ikonen

    Article

    Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

    Insert

    AFB2 (AFB2 Mustard Weed)
    

    Use

    CRISPR and TALEN; Human safe harbor locus (a avs1…

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    EF1a

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. CiDr-VSP L223F-mCherry pcDNA3.1 (eVSP)

    Plasmid

    #140892

    Purpose

    Expresses modified Danio rerio VSP without phosphatase activity (inactive mutant of enhanced VSP) fused with mCherry in mammalian cells.

    Depositor

    Yasushi Okamura

    Article

    Kawanabe et al J Gen Physiol. 2020 May 4;152(5). pii: 1

    Insert

    CiDr-VSP L223F-mCherry (tpte, vsp Zebrafish, Ciona intestinalis)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    changed 223L to F in Dr-VSP (zebrafish TPTE)

    Promoter

    Available Since

    April 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLVH-EF1a-GFP-P2A-MYC

    Plasmid

    #130695

    Purpose

    Lentiviral plasmids encoding human MYC with co-expressoin of GFP mediated by P2A.

    Depositor

    Kejin Hu

    Article

    Shao et al Nat Commun. 2016 Mar 7;7:10869. doi: 10.

    Insert

    human MYC (MYC Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDEST47-SAR1-GFP

    Plasmid

    #67409

    Purpose

    Mammalian expression of hSar1 with C-term GFP tag

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    SAR1 (SAR1A Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only