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We narrowed to 10,500 results for: fus

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Showing: 1881 - 1920 of 10500 results

  1. Cx43-msfGFP

    Plasmid
    #69007
    Purpose
    Expresses rat Cx43 (Gja1 CDS) with a 7 amino acid linker on the C-terminus of the Connexin linking to monomerized superfolder Green Fluorescent Protein. Expression driven by CMV promoter.
    Depositor
    David Spray
    Article
    Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.
    Insert
    Gja1 (Gja1 Rat)
    Tags
    monomerized superfolder GFP
    Expression
    Mammalian
    Promoter
    cmv
    Available Since
    Oct. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    69007-plasmid-map-sequence-id-121423

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  9. SpySwitch

    Plasmid
    #184225
    Purpose
    Expresses SpySwitch in the bacterial cytoplasm. SpySwitch binds SpyTag-, SpyTag002- or SpyTag003-fusions non-covalently for affinity purification, allowing gentle pH or temperature release.
    Depositor
    Mark Howarth
    Article
    Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:
    Insert
    SpySwitch
    Tags
    His6
    Expression
    Bacterial
    Mutation
    E77A mutation prevents isopeptide bond formation,…
    Promoter
    T7
    Available Since
    June 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    184225-plasmid-map-sequence-id-361604

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  15. pSCM001

    Plasmid
    #124605
    Purpose
    Expresses mCherry-pHluorin fusion protein in E. coli, a fluorescent reporter for cytoplasmic pH
    Depositor
    David Summers
    Article
    Zarkan et al Sci Rep. 2019 Mar 7;9(1):3868. doi: 10.1
    Insert
    mCherry-pHluorin
    Tags
    mCherry-(Gly-Gly-Ser)x2-pHluorin translational fu…
    Expression
    Bacterial
    Promoter
    pDAB
    Available Since
    May 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    124605-plasmid-map-sequence-id-242761

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  18. pAC149-pCR8-dCas9VP160

    Plasmid
    #48221
    Purpose
    dCas9VP160 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression
    Depositor
    Rudolf Jaenisch
    Article
    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2
    Insert
    dCas9(D10A;H840A) fusion with VP160 activation domain
    Use
    CRISPR and Gateway Cloning
    Tags
    HA Tag and VP160
    Mutation
    D10A;H840A nuclease-deficient
    Available Since
    Sept. 17, 2013
    Availability
    Academic Institutions and Nonprofits only
    48221-plasmid-map-sequence-id-69506

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  24. pcDNA3.1+ SMN1 myc HIS

    Plasmid
    #71687
    Purpose
    Mammalian expression of SMN1
    Depositor
    Gideon Dreyfuss
    Insert
    SMN1 (SMN1 Human)
    Tags
    HIS and myc
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    71687-plasmid-map-sequence-id-287410

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  37. pSH62-EBD

    Plasmid
    #49455
    Purpose
    yeast expression of Cre recombinase fused to the estrogen binding domain (EBD), which confers estradiol-dependent control to Cre recombinase
    Depositor
    Marc Gartenberg
    Article
    Cheng et al Nucleic Acids Res. 2000 Dec 15;28(24):E1
    Insert
    Cre-EBD fusion
    Use
    Cre/Lox
    Expression
    Yeast
    Promoter
    Gal1 promoter
    Available Since
    Dec. 5, 2013
    Availability
    Academic Institutions and Nonprofits only
    49455-plasmid-map-sequence-id-73668

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Showing: 1881 - 1920 of 10500 results