We narrowed to 3,272 results for: aaas

1. pFusX2-1

   Plasmid

   #63283

   Purpose

   Encodes RVD positions 4-6 in TALEN assembly

   Depositor

   Stephen Ekker

   Article

   Ma et al Hum Gene Ther. 2016 Jun;27(6):451-63. do

   Insert

   NI NI NI
   

   Use

   TALEN

   Tags

   Expression

   Mutation

   none

   Promoter

   Available since

   March 31, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pFusX1-1

   Plasmid

   #63219

   Purpose

   Encodes RVD positions 1-3 in TALEN assembly

   Depositor

   Stephen Ekker

   Article

   Ma et al Hum Gene Ther. 2016 Jun;27(6):451-63. do

   Insert

   NI NI NI
   

   Use

   TALEN

   Tags

   Expression

   Mutation

   none

   Promoter

   Available since

   March 31, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pLL3.7 Alk.2 shRNA

   Plasmid

   #59297

   Purpose

   shRNA to mouse Alk

   Depositor

   Ulrike Heberlein

   Article

   Lasek et al J Neurosci. 2011 Oct 5;31(40):14134-41.

   Insert

   Alk.2 shRNA
   

   Use

   Cre/Lox, Lentiviral, and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mouse U6

   Available since

   Aug. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

4. GLRX3

   Plasmid

   #39036

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   GLRX3 (GLRX3 Human)
   

   Use

   Tags

   His Tev

   Expression

   Bacterial

   Mutation

   A PCR artifact adds a C-terminal sequence includi…

   Promoter

   Available since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pSUPER.retro.puro-THAP11-E

   Plasmid

   #36995

   Depositor

   Debu Chakravarti

   Article

   Parker et al Mol Cell Biol. 2012 May;32(9):1654-70. E

   Insert

   THAP11 shRNA (THAP11 Human)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 15, 2012

   Availability

   Academic Institutions and Nonprofits only

6. pDD428

   Plasmid

   #202081

   Purpose

   Cas9/sgRNA plasmid targeting the C-terminus of Myh9

   Depositor

   Daniel Dickinson

   Article

   Shi et al Development. 2023 May 15;150(10):dev2013

   Insert

   pEF1a-mCherry-Cas9 + Myh9 CT sgRNA (Myh9 S. pyogenes (Cas9))
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Halo-TCAB1 KI sgRNA

   Plasmid

   #207611

   Purpose

   sgRNA for HaloTag insertion into the endogenous TCAB1 locus

   Depositor

   Jens Schmidt

   Article

   Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

   Insert

   CCAAAGTCTTCATACTGCAG
   

   Use

   Tags

   None

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. RfxCas13d-backbone

   Plasmid

   #165078

   Purpose

   Expression of RfxCas13d sgRNAs from a U6 promoter

   Depositor

   Ling-Ling Chen

   Article

   Li et al Nat Methods. 2021 Jan;18(1):51-59. doi:

   Insert

   Scaffold gRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLKO-PTEN-shRNA-1320

   Plasmid

   #25638

   Depositor

   Todd Waldman

   Article

   Kim et al Mol Cell Biol. 2007 Jan . 27(2):662-77.

   Insert

   PTEN-shRNA-1320 (PTEN Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 21, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLKO-shBRCA1 #2

    Plasmid

    #44595

    Depositor

    Eros Lazzerini Denchi

    Article

    Okamoto et al Nature. 2013 Feb 6. doi: 10.1038/nature1

    Insert

    BRCA1 (Brca1 Mouse)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 7, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pGreenPuro-shRNA-Stx3S-C4

    Plasmid

    #99742

    Purpose

    3rd generation lentiviral vector expresses shRNA against human Stx3S with a GFP reporter.

    Depositor

    Thomas Weimbs

    Article

    Giovannone et al J Biol Chem. 2018 Apr 13;293(15):5478-54

    Insert

    shRNA against Stx3S
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1

    Available since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. px458_2A_GFP_sgRNA_TIAL1

    Plasmid

    #106096

    Purpose

    CRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting TIAL1

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

    Insert

    gRNA TIAL1
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCgsgRNA_crtYf

    Plasmid

    #153338

    Purpose

    Double-plasmid-based CRISPR-Cas9 system in Corynebacterium glutamicum; rep oriVC. glutamicum; pMB1 oriVE. Coli; Pj23119-crRNA targeting crtYf; Spcr

    Depositor

    Sheng Yang

    Article

    Zhang et al ACS Synth Biol. 2020 Jul 17;9(7):1897-19

    Insert

    crRNA targeting crtYf
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pC0051-W85X REPAIR targeting guide cloned into pC0043

    Plasmid

    #103867

    Purpose

    Guide RNA expression plasmid for dPspCas13b that can be used to direct ADAR deaminase domain activity to the W85X mutation in the luciferase reporter plasmid pC0038

    Depositor

    Feng Zhang

    Article

    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

    Insert

    W85X targeting guide RNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLentiCRISPRv2-sgMaob-HepmCherry

    Plasmid

    #192829

    Purpose

    Expresses sgRNA targeting Maob and mCherry from hepatocyte-specific promoter

    Depositor

    Kristin Knouse

    Article

    Keys et al Cell Genom. 2022 Dec 14;2(12):100217. do

    Insert

    mCherry
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 for sgRNA and hepatocyte-specific promoter (HS…

    Available since

    Nov. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. mEmerald-MAP4-C-10

    Plasmid

    #54152

    Purpose

    Localization: Microtubules, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Article

    Planchon et al Nat Methods. 2011 May;8(5):417-23. doi:

    Insert

    MAP4
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    aa 640-1045 of MAP4

    Promoter

    CMV

    Available since

    July 10, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

17. Mef2A-shRNA

    Plasmid

    #32899

    Depositor

    Michael Greenberg

    Article

    Flavell et al Science. 2006 Feb 17;311(5763):1008-12.

    Insert

    Mef2a (Mef2a Rat)
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1-RNA

    Available since

    Nov. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pYZ173

    Plasmid

    #98410

    Purpose

    Cas9-gRNA lys9 plasmid for lys9 deletion in S. pombe

    Depositor

    Jef Boeke

    Article

    Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

    Insert

    gRNA targeting Sp.lys9 (SPBC3B8.03 Fission Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSLQ5135: pCAG-BFP-GuideArray

    Plasmid

    #210606

    Purpose

    Guide array for hyperdCas12a-based transcriptional activator with AAAT synSeparator

    Depositor

    Stanley Qi

    Article

    Liu et al Nat Commun. 2023 Oct 18;14(1):6575. doi:

    Insert

    pCAG-BFP-GuideArray
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    Dec. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CROP_C9_Puro_PARP1

    Plasmid

    #183312

    Purpose

    All-in-One CRISPRko system with a guide RNA that targets PARP1 gene

    Depositor

    Prashant Mali

    Article

    Hu et al Biomaterials. 2022 Jan;280:121276. doi:

    Insert

    PARP1
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. PX458_TEAD1_1

    Plasmid

    #86356

    Purpose

    Encodes gRNA for 3' target of human TEAD1

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA against TEAD1 (TEAD1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pX335-NEAT1_IS_v2

    Plasmid

    #97086

    Purpose

    Encodes an sgRNA that creates a SSB near the poly(A) site of human NEAT1_1 isoform.

    Depositor

    Archa Fox

    Article

    Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

    Insert

    sgRNA NEAT1_IS_v2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAAV2-mtIF3 shRNA_TagRFP657

    Plasmid

    #182367

    Purpose

    shRNA targeting mtIF3 mRNA

    Depositor

    Chunghun Lim

    Article

    Lee et al BMC Biol. 2022 Jan 7;20(1):12. doi: 10.1

    Insert

    mtIF3 (Mtif3 Mouse)
    

    Use

    AAV and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. 2X_pX458_pSpCas9(BB)-2A-GFP_SOX17_bKO

    Plasmid

    #172225

    Purpose

    Cas9-2A-GFP expression vector bearing two sgRNAs targeting the centromeric human SOX17 CTCF-boundary.

    Depositor

    Alexander Meissner

    Article

    Wu et al Nat Commun. 2021 Aug 12;12(1):4897. doi:

    Insert

    sgRNA
    

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA6.2-GW/EmGFP-miR-SUMO23

    Plasmid

    #31073

    Depositor

    Wulf Paschen

    Article

    Yang et al Biochem Biophys Res Commun. 2009 Apr 24.

    Insert

    SUMO2/3 microRNA (SUMO2, SUMO3 Human)
    

    Use

    RNAi

    Tags

    EmGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. TET-pLKO.1 PURO shLuc

    Plasmid

    #83092

    Purpose

    Lentiviral shRNA vector for inducible knockdown of luciferase (control hairpin)

    Depositor

    Kevin Janes

    Article

    Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

    Insert

    shLuc
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

27. pColE1_sgRNA_M4NS_AmpR

    Plasmid

    #119156

    Purpose

    Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with four mismatches in the non-seed region

    Depositor

    Vincent Noireaux

    Article

    Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

    Insert

    sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with four mismatches in the non-seed region
    

    Use

    Crispr

    Tags

    Expression

    Mutation

    Promoter

    J23119

    Available since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLG1-puro Non-targeting sgRNA 1

    Plasmid

    #109002

    Purpose

    CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    Non-targeting sgRNA #1
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6 promoter

    Available since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. hU6 promoter PspCas13b gRNA dual luciferase reporter 3’UTR

    Plasmid

    #119859

    Purpose

    gRNA plasmid with hU6 promoter PspCas13b gRNA dual luciferase reporter

    Depositor

    Bryan Dickinson

    Article

    Rauch et al J Am Chem Soc. 2018 Sep 26;140(38):11974

    Insert

    gRNA dual luciferase reporter
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. plentiCRISPR-sgGOT1

    Plasmid

    #72874

    Purpose

    The lentiviral sgGOT1 vector was generated via ligation of hybridized oligos (below) into lentiCRISPR-v1 vector linearized with BsmBI using Gibson assembly (NEB).

    Depositor

    David Sabatini

    Article

    Birsoy et al Cell. 2015 Jul 30;162(3):540-51. doi: 10

    Insert

    sgGOT1
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 4, 2016

    Availability

    Academic Institutions and Nonprofits only

31. CROP_C9_Puro_TOP1

    Plasmid

    #183323

    Purpose

    All-in-One CRISPRko system with a guide RNA that targets TOP1 gene

    Depositor

    Prashant Mali

    Article

    Hu et al Biomaterials. 2022 Jan;280:121276. doi:

    Insert

    TOP1
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pC1.530

    Plasmid

    #205441

    Purpose

    Level 1 Position 1 pUC19 vector with sgRNA and GmR. Used for homologous recombination with Cas12a editing vector pC1.509. sgRNA targets pC1.509 for removal.

    Depositor

    Alistair McCormick

    Article

    Victoria et al Plant Physiol. 2024 May 7:kiae261. doi:

    Insert

    RepB gRNA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. CROP_C9_Puro_FLT3

    Plasmid

    #183293

    Purpose

    All-in-One CRISPRko system with a guide RNA that targets FLT3 gene

    Depositor

    Prashant Mali

    Article

    Hu et al Biomaterials. 2022 Jan;280:121276. doi:

    Insert

    FLT3
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pT7-th-sgRNA

    Plasmid

    #65565

    Purpose

    in vitro trancription of sgRNA targeting the zebrafish th locus

    Depositor

    Jiu-lin Du

    Article

    Li et al Cell Res. 2015 Apr 7. doi: 10.1038/cr.20

    Insert

    th sgRNA (th Zebrafish)
    

    Use

    In vitro rna transcription

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pSF4 CMV intron renilla TRICK CTE polyA

    Plasmid

    #84443

    Purpose

    TRICK reporter mRNA

    Depositor

    Jeffrey Chao

    Article

    Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

    Insert

    Renill-TRICK
    

    Use

    Luciferase; Frt

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tet-CMV

    Available since

    Nov. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pLKO.1 EB1 shRNA #3

    Plasmid

    #37927

    Depositor

    Torsten Wittmann

    Article

    Gierke et al Curr Biol. 2012 May 8;22(9):753-62. Epub

    Insert

    EB1 shRNA #3 (MAPRE1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6

    Available since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pLV-H1-SGIPZ_NF1 sh1miR

    Plasmid

    #119206

    Purpose

    knockdown of NF1

    Depositor

    Helen Morrison

    Article

    Cui et al Hum Mutat. 2018 Nov 8. doi: 10.1002/humu

    Insert

    sh1miR targeting NF1 (NF1 Rat, Human, Mouse)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pARNTL.1.0-gDNA

    Plasmid

    #112480

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ARNTL

    Depositor

    Kevin White

    Insert

    ARNTL (BMAL1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. CROP_C9_Puro_PDGFRB

    Plasmid

    #183314

    Purpose

    All-in-One CRISPRko system with a guide RNA that targets PDGFRB gene

    Depositor

    Prashant Mali

    Article

    Hu et al Biomaterials. 2022 Jan;280:121276. doi:

    Insert

    PDGFRB
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pABM-D4-miR30-L1221

    Plasmid

    #132933

    Purpose

    Lentivector for luciferase (control) knockdown and blasticidin resistance

    Depositor

    Jeremy Luban

    Article

    Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

    Insert

    luciferase shRNA
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Oct. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge