Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 4,645 results for: PEP

Clear keywords
Showing: 2401 - 2450 of 4645 results

  5. pZF-ADD-L

    Plasmid
    #127477
    Purpose
    coding sequence for synthetic zinc finger ZF-ADD-L
    Depositor
    Sebastian Maerkl
    Article
    Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11
    Insert
    ZF-ADD synthetic transcription factor fused with PDZ cognate peptide ligand (VKESLV)
    Use
    Cell-free transcription–translation
    Tags
    PDZ cognate peptide ligand (VKESLV)
    Promoter
    T7
    Available Since
    Nov. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    127477-plasmid-map-sequence-id-263301

    Enlarge

  6. pSB1C3_mTq2-Tat_YPet-PCP (cy-plasmid)

    Plasmid
    #127636
    Purpose
    Encodes two fluorecent proteins with RNA binding peptides: mTurquoise2-Tat and YPet-PCP. Expression with constitutive E. coli RNAP promoter (J23106), ribozymes RiboJ10 (for YPet) and PlmJ (for mTq2)
    Depositor
    Friedrich Simmel
    Article
    Schwarz-Schilling et al Nano Lett. 2018 Apr 11;18(4):2650-2657.
    Insert
    mTurquoiuse2-Tat, YPet-PCP
    Use
    Synthetic Biology
    Tags
    RNA binding peptide: PCP and RNA binding peptide:…
    Expression
    Bacterial
    Available Since
    July 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    127636-plasmid-map-sequence-id-251704

    Enlarge

  9. pKB236

    Plasmid
    #170027
    Purpose
    Encodes LacI-controlled expression of surface-displayed TCP and PvirK-mNeonGreen reporter
    Depositor
    Jeffrey Tabor
    Article
    Brink et al bioRxiv
    Inserts
    mNeonGreen
    TCP
    Use
    Synthetic Biology
    Tags
    Lpp, OmpA, and Tether
    Expression
    Bacterial
    Promoter
    Ptac and PvirK
    Available Since
    July 31, 2025
    Availability
    Academic Institutions and Nonprofits only
    170027-plasmid-map-sequence-id-335297

    Enlarge

  10. pKB238

    Plasmid
    #170033
    Purpose
    Encodes LacI-controlled expression of surface-displayed NPY and PvirK-mNeonGreen reporter
    Depositor
    Jeffrey Tabor
    Article
    Brink et al bioRxiv
    Inserts
    mNeonGreen
    Neuropeptide Y (NPY Synthetic, Escherichia coli, Human)
    Use
    Synthetic Biology
    Tags
    Lpp, OmpA, and Tether
    Expression
    Bacterial
    Promoter
    Ptac and PvirK
    Available Since
    July 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    170033-plasmid-map-sequence-id-336781

    Enlarge

  12. SL539

    Plasmid
    #49948
    Purpose
    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding site
    Depositor
    Keith Joung
    Article
    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038
    Insert
    RHOXF2 Rhox homeobox family member 2 (RHOXF2 Human)
    Use
    TALEN
    Promoter
    EF1a
    Available Since
    Jan. 21, 2014
    Availability
    Academic Institutions and Nonprofits only

  13. CUP1-∆ssCPY*-mGFP in pRS315

    Plasmid
    #221158
    Purpose
    Fluorescent reporter for cytoplasmic aggregates under LEU2 selection marker
    Depositor
    Mark Leake
    Insert
    Carboxypeptidase Y (PRC1 Budding Yeast)
    Tags
    mGFP
    Expression
    Yeast
    Mutation
    Deletion signal peptide, G255A mutation
    Available Since
    Nov. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    221158-plasmid-map-sequence-id-450962

    Enlarge

  17. pENT-L1-Kozak-EGFP-GPI-stop-L2

    Plasmid
    #186355
    Purpose
    Entry clone with ORF encoding plasma membrane-targeted EGFP-GPI fusion protein flanked by Gateway recombination sequences
    Depositor
    Patrick Lemaire
    Article
    Roure et al PLoS One. 2007. 2(9):e916.
    Insert
    signal peptide of human CD59, eGFP, aa 67-102 of human CD59 which contains the GPI attachment site (CD59 Synthetic, Human)
    Use
    Expression of a fluorescent membrane marker
    Tags
    EGFP
    Available Since
    Sept. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    186355-plasmid-map-sequence-id-373423

    Enlarge

  19. CUP1-∆ssCPY*-mGFP

    Plasmid
    #212197
    Purpose
    Fluorescent reporter for cytoplasmic aggregates
    Depositor
    Mark Leake, Chris MacDonald
    Insert
    Carboxypeptidase Y variant (PRC1 Budding Yeast)
    Tags
    mEGFP (monomeric version EGFP, A206K mutation)
    Expression
    Yeast
    Mutation
    Deletion signal peptide, G255A mutation
    Promoter
    CUP1
    Available Since
    Nov. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    212197-plasmid-map-sequence-id-450955

    Enlarge

  22. pFP35

    Plasmid
    #247210
    Purpose
    Expresses superfolder GFP (sfGFP) and a Pepper RNA aptamer (DF30ppr) from a T7 promoter. Suitable for cell-free expression.
    Depositor
    Elizabeth Strychalski
    Article
    Piorino et al ACS Synth Biol. 2026 Jan 16;15(1):243-26
    Inserts
    superfolder GFP
    Dimeric Pepper RNA aptamer (with F30 scaffold)
    Use
    Synthetic Biology; E. coli-based cell-free expres…
    Tags
    Streptavidin tag
    Expression
    Bacterial
    Mutation
    Two mutations were made to the second Pepper unit…
    Promoter
    T7
    Available Since
    April 27, 2026
    Availability
    Academic Institutions and Nonprofits only
    247210-plasmid-map-sequence-id-507798

    Enlarge

  23. pPB-PGK-mCherry.OVA(323-339)

    Plasmid
    #190005
    Purpose
    Piggybac construct for constitutive expression of mCherry and Chicken Ovalbumin OVA323-339 peptide fusion
    Depositor
    Siyuan Zhang
    Insert
    mCherry.OVA(323-339)
    Use
    Synthetic Biology; Piggybac
    Tags
    OVA(323-339)
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    July 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    190005-plasmid-map-sequence-id-382848

    Enlarge

  25. pNAS1b split mCherry NEDD4 WW2 pos ctrl

    Plasmid
    #112029
    Purpose
    Mode #2 phosphosite positive control (split mCherry, using NEDD4 WW2 binding domain paired with proline-rich positive control peptide)
    Depositor
    Jesse Rinehart
    Article
    Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.
    Insert
    NEDD4 WW2 domain fused to C-terminal split mCherry, proline-rich pos ctrl interacting peptide fused to N-terminal split mCherry
    Tags
    Split mCherry
    Expression
    Bacterial
    Available Since
    Aug. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    112029-plasmid-map-sequence-id-213508

    Enlarge

  29. EBL1-bio

    Plasmid
    #47792
    Purpose
    Expresses enzymatically monobiotinylated full-length EBL1 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised EBL1
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available Since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47792-plasmid-map-sequence-id-68762

    Enlarge

  34. pcDNA3-sACE2v2.2-8his

    Plasmid
    #149663
    Purpose
    Mammalian expression plasmid for his-tagged sACE2 high affinity variant 2.2
    Depositor
    Erik Procko
    Article
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Tags
    Short GSG linker and 8his tag
    Expression
    Mammalian
    Mutation
    Extracellular, soluble protease domain (a.a. M1-D…
    Promoter
    CMV
    Available Since
    May 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    149663-plasmid-map-sequence-id-298413

    Enlarge

  38. pelB-MBP-P13-ss

    Plasmid
    #137065
    Purpose
    E. coli expression clone (T7lac promoter) for mature P13 (aa 22-179) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage
    Depositor
    Debra Hansen
    Article
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAC66426.1 (BB_0034 Borrelia burgdorferi B31)
    Tags
    PelB signal peptide + His10 + maltose binding pro…
    Expression
    Bacterial
    Mutation
    mature P13: lacks the P13 signal peptide
    Promoter
    T7lac
    Available Since
    April 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    137065-plasmid-map-sequence-id-291955

    Enlarge

  39. CelTOS-COMP-blac-flag-his

    Plasmid
    #111014
    Purpose
    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.
    Depositor
    Gavin Wright
    Article
    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi
    Insert
    cell traversal protein for ookinetes and sporozoites (CelTOS) (PF3D7_1216600 )
    Tags
    Exogenous signal peptide of mouse origin and rat …
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available Since
    Oct. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    111014-plasmid-map-sequence-id-224577

    Enlarge

  40. GRP94-COMP-blac-flag-his

    Plasmid
    #111008
    Purpose
    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.
    Depositor
    Gavin Wright
    Article
    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi
    Insert
    endoplasmin, putative (GRP94) (PFL1070c Synthetic)
    Tags
    Exogenous signal peptide of mouse origin and rat …
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available Since
    Oct. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    111008-plasmid-map-sequence-id-224562

    Enlarge

  41. MAEBL-COMP-blac-flag-his

    Plasmid
    #110963
    Purpose
    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.
    Depositor
    Gavin Wright
    Article
    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi
    Insert
    merozoite adhesive erythrocytic binding protein (MAEBL) (PF3D7_1147800.1 Synthetic)
    Tags
    Exogenous signal peptide of mouse origin and rat …
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available Since
    Sept. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    110963-plasmid-map-sequence-id-223595

    Enlarge

  42. pelB-MBP-RevA-ss

    Plasmid
    #137064
    Purpose
    E. coli expression clone (T7lac promoter) for mature RevA (aa 25-160) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage
    Depositor
    Debra Hansen
    Article
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAF07416.1 (revA Borrelia burgdorferi B31)
    Tags
    PelB signal peptide + His10 + maltose binding pro…
    Expression
    Bacterial
    Mutation
    lacks the RevA signal peptide and 5 residues (CKA…
    Promoter
    T7lac
    Available Since
    April 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    137064-plasmid-map-sequence-id-291953

    Enlarge

  44. pMini-CMV-NLS-R-IscB-VR4-NLS (D60A, H243E, H269N, R270Q)_T2A_mCherry_U6-ωRNA

    Plasmid
    #246431
    Purpose
    All-in-one plasmid. Expresses R-IscB in mammalian cells. ssRNase enhancing mutations (H243E, H269N, R270Q) included.
    Depositor
    Ailong Ke
    Article
    Xu et al Cell. 2025 Aug 18:S0092-8674(25)00854-2.
    Inserts
    O.gue IscB-VR4 (H243E, H269N, R270Q) with RuvC dead mutations (D60A) and delta-TID
    mCherry
    ωRNA
    Tags
    SV40 NLS, Thosea asigna virus 2A peptide, and nuc…
    Expression
    Mammalian
    Mutation
    changed Aspartic Acid 60 to Alanine, changed Hist…
    Promoter
    CMV and U6
    Available Since
    Jan. 6, 2026
    Availability
    Academic Institutions and Nonprofits only
    246431-plasmid-map-sequence-id-496263

    Enlarge

  45. Ath-HEN1::T2A::mCherry::TY1::H2B-pCFJ350-ttTi5605

    Plasmid
    #108362
    Purpose
    Expression of 2xFlag MYC tagged A.thaliana-HEN1 (Ath-HEN1, codon optimised for C. elegans) and mCherry under the promoter of choice in C. elegans
    Depositor
    Luisa Cochella
    Article
    Alberti et al Nat Methods. 2018 Feb 26. pii: nmeth.461
    Inserts
    Ath-HEN1 (HEN1 Mustard Weed, Codon optimised for C. elegans)
    mCherry
    his-15 (his-15 Nematode)
    unc-54 3' UTR
    Tags
    2xFlag, MYC, T2A peptide, and TY1 linker
    Expression
    Worm
    Promoter
    A rab-3:Ath-HEN1:unc-54 3' UTR was cloned as…
    Available Since
    May 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    108362-plasmid-map-sequence-id-209815

    Enlarge

  46. pIL6-PMEL-hbb

    Plasmid
    #239868
    Purpose
    Template for in vitro transcription of PMEL (gp100), with signal peptide replaced by the IL6 signal peptide, flanked by the human haemoglobin beta 5' and 3' UTRs.
    Depositor
    Catherine Jopling
    Article
    Smart et al Mol Ther Nucleic Acids. 2025 Jun 24;36(3
    Insert
    Full premelanosome coding sequence with IL6 signal peptide, flanked by human haemoglobin beta 5' and 3' UTRs (PMEL Human)
    Expression
    Mammalian
    Mutation
    Valine to Glycine change is at amino acid 26 of n…
    Promoter
    T7 (included as part of insert)
    Available Since
    July 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    239868-plasmid-map-sequence-id-477826

    Enlarge

  47. pH6-TEV-spTorA-GFP(C)

    Plasmid
    #169041
    Purpose
    GFP preceeded by the signal peptide of TorA (spTorA) with an N-terminal 6xHis tag and TEV cleavage site in pBAD24.
    Depositor
    Siegfried Musser
    Article
    Bageshwar et al PLoS One. 2021 Sep 9;16(9):e0256715. doi
    Insert
    H6-spTorA-GFP
    Tags
    6xHis, TEV cleavage site, and spTorA (signal pept…
    Expression
    Bacterial
    Mutation
    The mut3 GFP variant with 5 additional mutations …
    Promoter
    araBAD
    Available Since
    Oct. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    169041-plasmid-map-sequence-id-346121

    Enlarge

  50. CYP19B-COMP-blac-flag-his

    Plasmid
    #111007
    Purpose
    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.
    Depositor
    Gavin Wright
    Article
    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi
    Insert
    peptidyl-prolyl cis-trans isomerase (CYP19B) (CyP22 Synthetic)
    Tags
    Exogenous signal peptide of mouse origin and rat …
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available Since
    Oct. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    111007-plasmid-map-sequence-id-224561

    Enlarge

Showing: 2401 - 2450 of 4645 results