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We narrowed to 5,916 results for: pAAV

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Showing: 201 - 250 of 5916 results

  3. pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE

    Plasmid
    #125712
    Purpose
    Expresses an optimized mosquito OPN3 rhodopsin in-frame with mScarlet
    Depositor
    Ofer Yizhar
    Article
    Mahn et al Neuron. 2021 May 10. pii: S0896-6273(21)
    Has Service
    AAV Retrograde, AAV1, and AAV5
    Insert
    eOPN3
    Use
    AAV
    Tags
    Rho1D4 and mScarlet
    Expression
    Mammalian
    Mutation
    deleted amino acids 331-429
    Promoter
    short version of αCamKinase II promoter (0.4kb)
    Available Since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    125712-plasmid-map-sequence-id-247206

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  6. pAAV2/7

    Plasmid
    #112863
    Purpose
    AAV packaging plasmid expressing Rep/Cap genes
    Depositor
    James M. Wilson
    Insert
    Rep2/Cap7
    Use
    AAV
    Promoter
    Truncated P5
    Available Since
    Sept. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    112863-plasmid-map-sequence-id-364707

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  7. pAAV.CAG.hChR2(H134R)-mCherry.WPRE.SV40

    Plasmid
    #100054
    Purpose
    AAV expression of humanized ChR2 with H134R mutation fused to mCherry driven by CAG promoter for optogenetic activation
    Depositor
    Karl Deisseroth
    Has Service
    AAV1, AAV2, AAV5, and AAV9
    Insert
    channelrhodopsin-2
    Use
    AAV
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    H134R mutation in ChR2
    Promoter
    CAG
    Available Since
    Jan. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    100054-plasmid-map-sequence-id-183171

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  8. pAAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA

    Plasmid
    #154868
    Purpose
    Human synapsin-1 promoter; Flp-dependent hM3D(Gq) DREADD-mCherry
    Depositor
    Ulrik Gether
    Has Service
    AAV Retrograde and AAV8
    Insert
    hM3D(Gq)-mCherry
    Use
    AAV; Flp-frt
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    Human Synapsin-1
    Available Since
    Aug. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    154868-plasmid-map-sequence-id-308827

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  10. pAAV-nEF-Con/Fon DREADD Gi-mCherry

    Plasmid
    #177672
    Purpose
    Expresses DREADD hM4D (Gi) in Cre and Flp expressing cells
    Depositor
    Karl Deisseroth
    Has Service
    AAV8
    Insert
    DREADD Gi-mCherry
    Use
    AAV, Cre/Lox, and Mouse Targeting ; Intrsect
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    nEF
    Available Since
    Nov. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    177672-plasmid-map-sequence-id-348533

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  12. pAAV.Syn.Flex.GCaMP6m.WPRE.SV40

    Plasmid
    #100838
    Purpose
    AAV expression of Cre recombinase-activated ultrasensitive protein calcium sensor from Synapsin promoter
    Depositor
    Douglas Kim, GENIE Project
    Article
    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d
    Has Service
    AAV1 and AAV9
    Insert
    GCaMP6m
    Use
    AAV
    Expression
    Mammalian
    Mutation
    GCaMP3-T302L R303P M378G K379S D380Y T381R S383T …
    Promoter
    Synapsin
    Available Since
    Nov. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    100838-plasmid-map-sequence-id-198300

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  16. pAAV-CAG-SomaGCaMP6f2

    Plasmid
    #158757
    Purpose
    Cell body-targeted GCaMP6f, under CAG promoter: GCaMP6f followed by a linker, and the EE-RR coiled coil motif (GCaMP6f-27-EE-RR). As bright as conventional GCaMP6f.
    Depositor
    Edward Boyden
    Article
    Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)
    Has Service
    AAV8 and AAV9
    Insert
    SomaGCaMP6f2
    Use
    AAV
    Expression
    Mammalian
    Promoter
    CAG promoter
    Available Since
    Sept. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    158757-plasmid-map-sequence-id-310662

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  17. pAAV-CaMKIIa-HA-hM4D(Gi)-IRES-mCitrine

    Plasmid
    #50467
    Purpose
    Gi-coupled hM4D-IRES-mCitrine under the control of CaMKIIa promoter
    Depositor
    Bryan Roth
    Has Service
    AAV2 and AAV8
    Insert
    hM4D(Gi)-IRES-mCitrine (CHRM4 Human)
    Use
    AAV
    Tags
    HA
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    CaMKIIa
    Available Since
    April 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    50467-plasmid-map-sequence-id-199198

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  19. pAAV2/rh10

    Plasmid
    #112866
    Purpose
    AAV packaging plasmid expressing Rep/Cap genes
    Depositor
    James M. Wilson
    Insert
    Rep2/Cap-rh10
    Use
    AAV
    Promoter
    Truncated P5
    Available Since
    Sept. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    112866-plasmid-map-sequence-id-339914

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  23. pAAV-hSyn-DIO-ChrimsonR-mRuby2-ST

    Plasmid
    #105448
    Purpose
    Cation channelrhodopsin ChrimsonR fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites in a viral vector
    Depositor
    Hillel Adesnik
    Article
    Pegard et al Nat Commun. 2017 Oct 31;8(1):1228. doi:
    Has Service
    AAV9
    Insert
    ChrimsonR-mRuby2-ST
    Use
    AAV
    Tags
    mRuby2 and soma targeting motif
    Mutation
    K176R in Chrimson
    Promoter
    hSyn
    Available Since
    Feb. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    105448-plasmid-map-sequence-id-448714

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  28. pAAV-CAG-FLEX-rc [Jaws-KGC-tdTomato-ER2]

    Plasmid
    #84446
    Purpose
    AAV-mediated expression of Jaws-KGC-tdTomato-ER2 under the CAG promoter, in floxed/reversed (Cre-dependent) manner.
    Depositor
    Edward Boyden
    Article
    Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi
    Has Service
    AAV1, AAV5, and AAV8
    Insert
    Jaws-KGC-tdTomato-ER2
    Use
    AAV
    Tags
    ER2, KGC, and tdTomato
    Expression
    Mammalian
    Mutation
    K200R W214F
    Promoter
    CAG
    Available Since
    April 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    84446-plasmid-map-sequence-id-317050

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  39. pAAV-hSyn-DIO-jGCaMP8s-P2A-ChrimsonR-ST

    Plasmid
    #174007
    Purpose
    Cre dependent co-expression of jGCaMP8s and soma-targeted ChrimsonR under the control of an hSyn promoter. jGCaMP8s and ChrimsonR are separated by cleavable peptide sequence P2A.
    Depositor
    Mark Histed
    Article
    LaFosse et al eNeuro. 2023 Mar 29;10(3):ENEURO.0378-22
    Has Service
    AAV9
    Insert
    jGCaMP8s-P2A-ChrimsonR-ST
    Use
    AAV
    Tags
    6xHis and soma targeting motif
    Promoter
    hSyn
    Available Since
    May 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    174007-plasmid-map-sequence-id-361587

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  42. pAAV-CAG-DIO-ChR2(H134R)-eYFP

    Plasmid
    #127090
    Purpose
    Cre-dependent AAV expression of humanized ChR2 (with H134R mutation) fused to eYFP for optogenetic activation
    Depositor
    Viviana Gradinaru
    Article
    Rajendran et al Nat Commun. 2019 Apr 26;10(1):1944. doi:
    Has Service
    AAV PHP.eB
    Insert
    Channelrhodopsin-2
    Use
    AAV
    Tags
    eYFP (C terminal on insert)
    Expression
    Mammalian
    Mutation
    Humanized ChR2 gene with histidine 134 changed to…
    Promoter
    CAG
    Available Since
    June 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    127090-plasmid-map-sequence-id-246739

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  45. pAAVS1-NDi-CRISPRi (Gen1)

    Plasmid
    #73497
    Purpose
    Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus with mCherry marker
    Depositor
    Bruce Conklin
    Article
    Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.
    Inserts
    dCas9-KRAB-P2A-mCherry
    rtTA
    Use
    CRISPR
    Tags
    HA, KRAB, and NLS
    Expression
    Mammalian
    Mutation
    D10A, H840A (catalytically deactivated Cas9)
    Promoter
    CAG and TRE3G
    Available Since
    Dec. 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    73497-plasmid-map-sequence-id-247304

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Showing: 201 - 250 of 5916 results