169,913 results
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pLumi-LgBiT
Plasmid#188319PurposeExpression of LgBiT via sigma 70 promoter in E. coliDepositorInsertLgBiT
UseLuciferase and Synthetic BiologyExpressionBacterialPromotersigma 70 promoterAvailable SinceSept. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
LAMP1-G-GECO1.2
Plasmid#240467PurposeMid-affinity GECI targeted to cytosolic face of the lysosomeDepositorInsertLAMP1 (LAMP1 Human)
TagsG-GECO1.2ExpressionMammalianMutationSilent mutationPromoterCMVAvailable SinceOct. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
CMV-LgBit
Plasmid#234738PurposeMammalian expression of large NanoBit fragment (LgBit)DepositorInsertLgBit
ExpressionMammalianPromoterCMVAvailable SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDH-cGAS-E225A D227A-mCherry2-EF1-Puro
Plasmid#132771PurposeInteracts with cytoplasmic DNA. Useful for identifying sites of nuclear envelope rupture.DepositorInsertCyclic GMP-AMP synthase (CGAS Human)
UseLentiviralTagsmCherry2ExpressionMammalianMutationE225A D227AAvailable SinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAVS1 T2 CRIPR in pX330
Plasmid#72833PurposeCRISPR/Cas to target the AAVS1 locus in human cellsDepositorInsertCas9 and gRNA for targeting the AAVS1 locus in human cells
UseCRISPRExpressionMammalianPromoterU6 promoterAvailable SinceFeb. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
EB3N-VVDfast-mCl3-iLID
Plasmid#190165PurposeExpresses EB3N-VVDfast-mCl3-iLID, a microtubule anchor used for optogenetic recruitment of SspB-mCh-p60, in mammalian cells.DepositorInsertEB3N-VVDfast-mClover3-iLID
Tags-VVDfast-mClover3-iLIDExpressionMammalianMutation1-189 aa from EB3Available SinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1 Full length Importin beta
Plasmid#106941PurposeTransient expression of GFP-tagged Importin beta in mammalian cells (CMV promoter)DepositorInserthuman Importin beta-1 (KPNB1) (KPNB1 Human)
TagsGFPExpressionMammalianPromoterCMV promoterAvailable SinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMJ915
Plasmid#69090PurposeExpress Streptococcus pyogenes Cas9 carrying two C-terminal SV40 NLSDepositorInsertcas9
TagsMBP, hexa His, and two SV40 NLSExpressionBacterialPromoterT7Available SinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
Venus-rab5a
Plasmid#234742PurposeMammalian expression of early endosome marker Venus-rab5aDepositorAvailable SinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
1036 pcDNA3 Myr HA Akt1
Plasmid#9008PurposeMammalian expression of human Akt1 with myr, HA tagsDepositorAvailable SinceNov. 2, 2005AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Gamma-Tubulin-17
Plasmid#55050PurposeLocalization: Centrosomes, Excitation: 587, Emission: 610DepositorAvailable SinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
TRPML1-G-GECO1.2-ERES
Plasmid#207144PurposeFusion of Ca2+ reporter and lysosomal Ca2+-permeable channel, with ER-export signalDepositorInsertMcoln1 (MCOLN1 Human)
TagsER-Export Signal (ERES) and G-GECO1.2ExpressionMammalianPromoterCMVAvailable SinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHN764
Plasmid#178819PurposeExpression of EndoH in E. coliDepositorInsertsynthetic gene encoding endo-β-N-acetylglucosaminidase H
TagsHIs8 and MBPExpressionBacterialPromotertacAvailable SinceJan. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PEmax-P2A-GFP
Plasmid#180020PurposeMammalian expression of SpCas9 PEmax prime editor with P2A-EGFP markerDepositorInsertPEmax-P2A-EGFP
TagsP2A-EGFP, SV40 bpNLS, and c-Myc NLSExpressionMammalianMutationDetailed in manuscriptPromoterCMVAvailable SinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pJT260 Lenti pEF-dCas9-3XFLAG-VPR-T2A-tagBFP-P2A-Blast
Plasmid#187336PurposedCas9 effector fusion for manipulating transcriptionDepositorInsertdCas9-3XFLAG-VPR-T2A-tagBFP-P2A-Blast
UseCRISPR and LentiviralTags3XFLAGExpressionMammalianPromoterpEF1aAvailable SinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
SpyTag003-MBP
Plasmid#133450PurposeExpresses SpyTag003-MBP (maltose binding protein) in bacterial cytoplasmDepositorInsertSpyTag003-MBP
TagsHis6 and Thrombin cleavage siteExpressionBacterialMutationPeptide tag added to N-terminus of MBP via a GSGE…PromoterT7Available SinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLKO-TRE-AFOS
Plasmid#197079PurposeA doxycycline inducible dominant negative form of transcription complex AP1. AFOS heterodimerizes and inhibits c-Jun. Plasmid contains PGK driven H2B-RFP as a fluorescent reporter of transduction.DepositorAvailable SinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF2546
Plasmid#144209PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only
