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Plasmid#54095PurposeLocalization: Actin Bundling Protein, Excitation: 487, Emission: 509DepositorInsertFascin (FSCN1 Human)
UseTagsmEmeraldExpressionMammalianMutationPromoterCMVAvailable sinceJune 16, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCDNA5/FRT/TO-[4xCox8]-ChemoG-NAD
Plasmid#193823PurposeCMV overexpression of ChemoG-NAD in the mitochondria of mammalian cell linesDepositorInsert[4xCox8]-ChemoG-NAD
UseTagsExpressionMammalianMutationEGFP:A206K, T225R; HT7: L271EPromoterCMVAvailable sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEIAV-SIN6.1 CBGFPW
Plasmid#44173DepositorInsertenhanced green fluorescent protein
UseLentiviralTagsExpressionMutationchromophore mutationsPromoterCB, CMV enhancer/chicken beta-actin promoterAvailable sinceMay 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX-TEVsite-human Glomulin
Plasmid#52292Purposebacterial expression of human GlomulinDepositorInsertGlomulin (GLMN Human)
UseTagsGST and TEVExpressionBacterialMutationPromotertacAvailable sinceMarch 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPaxillin-Electra1-N1
Plasmid#179484PurposeExpresses paxillin fused to blue fluorescent protein Electra1DepositorInsertpaxillin-Electra1
UseTagsElectra1ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIC111
Plasmid#44435DepositorTypeEmpty backboneUseRetroviralTagsEGFP, His, and PreScission cleavage site (2x)ExpressionMammalianMutationPromoterAvailable sinceApril 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDECKO_GFP
Plasmid#141210Purposeempty GFP vector for cloning sgRNA or pgRNAsDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
EK0379 CMV-TO-EGFP-stop-t70587 (PB)
Plasmid#191154PurposeExpression of Trigger 1 in the 3' UTR of EGFP in mammalian cells; used to make a cell line to study sensors for integrated 3' UTR or CDS triggers; expression is inducible with doxycycline due to CMV-TO promoter.DepositorInsertEGFP-t70587
UseTagsExpressionMammalianMutationWTPromoterCMV-TOAvailable sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEpicVector3mC_Puro-T2A-mCherry-VSVg-StrepTagII-HA
Plasmid#162100PurposemCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.DepositorInsertPuromycin resistant; mCherry linked to VSVg-StrepTagII-HA
UseLentiviralTagsVSVg-StrepTagII-HAExpressionMutationWTPromoterEF1AAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
RRL.sin.cPPT.SFFV/Flag-codon-optimized-NCOA7 variant 6.IRES-puro.WPRE (CG584)
Plasmid#139444PurposeLentiviral vector to ectopically express human codon-optimized NCOA7 isoform 4DepositorInsertFLAG-tag codon-optimized NCOA7 variant 6 (coding for isoform 4, the short, interferon-inducble isoform) (NCOA7 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationsequence codon-optimizedPromoterSFFVAvailable sinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT-ChemoL-CaM
Plasmid#193830PurposeCMV overexpression of ChemoL-CaM in mammalian cell linesDepositorInsertChemoL-CaM
UseTagsExpressionMammalianMutationEGFP:A206K, E299D; HT7: L271EPromoterCMVAvailable sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-28a RUVBL2 Full Length
Plasmid#159142PurposeExpresssion and protein purification of full length RUVBL2DepositorInsertRUVBL2 (RUVBL2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGreenII 0229/ProRUBY:GOXL1-Citrine:NOSt
Plasmid#175563PurposepSOUP helper plasmid needed for Agrobacterium-mediated plant transformation - Expression of GOXL genes tagged with Citrine under RUBY promoter for transgene complementation of ruby-1DepositorInsertGOXL1
UseTagsCitrineExpressionPlantMutationPromoterRUBYAvailable sinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pre_IV_CS8_7569_v1
Plasmid#126865Purpose7886 bases long scaffold with Zn-dependent self-cleaving DNAzyme cassettes, from compatibility group 8, made by method IV, yields linear 7569 b long scaffolds after Zn incubationDepositorInsertIV_CS8_7569_v1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTrcHIS-7D12Gln13TAG
Plasmid#125264PurposeExpresses 7D12 anti-EGFR nanobodies with UAG codon at Gln13 for synthetic amino acid in combination with pEVOL-pAzFDepositorInsertGln13TAG 7D12
UseTagsHis-TagExpressionBacterialMutationPromoterAvailable sinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-V5-Bet1
Plasmid#120243PurposeExpresses V5-tagged Bet1 in lentiviral vectorDepositorInsertBet1 (Bet1 Mouse)
UseLentiviralTagsV5ExpressionMutationPromoterEF1aAvailable sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-CUL1 delta 610-615
Plasmid#19941DepositorInsertcullin 1 delta 610-615 (CUL1 Human)
UseTagsHAExpressionMammalianMutationCUL1 with a ROC1 binding mutantPromoterAvailable sinceApril 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAAV-MCS-shCHD5 #1
Plasmid#68876PurposeCHD5 shRNA expressed from Adeno-associated viral (AAV) vectorDepositorInsertCHD5
UseAAV and RNAiTagsExpressionMammalianMutationPromoterH1Available sinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
FgH1tUTG_mup53_Ex5
Plasmid#85535PurposeInducible expression of guide RNA (mup53_Ex5) with fluorescent GFP reporterDepositorInsertmu p53
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterH1tAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYTK066
Plasmid#65173PurposeEncodes tTDH1 as a Type 4b part to be used in the Dueber YTK systemDepositorInserttTDH1
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-CUL1 delta N53
Plasmid#19950DepositorInsertcullin 1 delta N53 (CUL1 Human)
UseTagsHAExpressionMammalianMutationdeletion of 53 residues from the NH2-terminus(de…PromoterAvailable sinceApril 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
pFL-SV40-p38 β/MAPK11 5'-UTR
Plasmid#115357Purposefirefly luciferase (Fluc) reporter containing the 5’-UTR of p38 β/MAPK11 mRNADepositorInsertp38 β/MAPK11 5'-UTR (MAPK11 Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-CUL1 delta C22
Plasmid#19942DepositorInsertcullin 1 delta C22 (CUL1 Human)
UseTagsHA2ExpressionMammalianMutationMutation of 22 amino acids from the C terminusPromoterAvailable sinceApril 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
PKD1 gRNA (BRDN0001149263)
Plasmid#76843Purpose3rd generation lentiviral gRNA plasmid targeting human PKD1DepositorInsertPKD1 (PKD1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-MCS-shCHD5 #2
Plasmid#68877PurposeCHD5 shRNA expressed from Adeno-associated viral (AAV) vectorDepositorInsertCHD5
UseAAV and RNAiTagsExpressionMammalianMutationPromoterH1Available sinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRK5 BMPS C231S-6xHis
Plasmid#204834PurposeMammalian expression of recombinant BMPS C231SDepositorInsertCLN5 (CLN5 Human)
UseTags6xHisExpressionMammalianMutationC231SPromoterCMVAvailable sinceMay 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRK5 BMPS WT-6xHis
Plasmid#204833PurposeMammalian expression of recombinant BMPS WTDepositorInsertCLN5 (CLN5 Human)
UseTags6xHisExpressionMammalianMutationPromoterCMVAvailable sinceMay 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
AAV-Syn-fDIO-mCherry-IRES-WGA-Cre
Plasmid#232905PurposeFlp-dependent anterograde transsynaptic tracerDepositorInsertSyn-fDIO-mCherry-IRES-WGA-Cre
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMay 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEB multi-Hyg Human collagen type IV alpha 4 chain (COL4A4)
Plasmid#229771Purpose3xFlag-tagged human Col4A4 to be used with N- or C-tagged LgBiT Col4A5 and SmBiT Col4A3 in Nanobit assay systemDepositorInsertHuman collagen type IV alpha 4 chain (COL4A4) (COL4A4 Human)
UseTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceMarch 31, 2025AvailabilityAcademic Institutions and Nonprofits only -
iGFP-gamma-actin
Plasmid#231554PurposeMammalian expression of human gamma actin fused intramolecularly to monomeric superfolder GFPDepositorInsertACTG1 (ACTG1 Human)
UseTagsmsfGFP (intramolecular)ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EF1a-HIF2a dPA (P405A, P531A)
Plasmid#232954PurposeStably express a constitutively stable version of HIF2a (HIF2a dPA)DepositorInsertHIF2a (EPAS1 Human)
UseLentiviralTagsExpressionMammalianMutationP405A, P531APromoterEF1aAvailable sinceMarch 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1-gfaABC1D-PinkyCaMP
Plasmid#232859PurposeAn AAV vector expressing the mScarlet based calcium indicator PinkyCaMP under gfaABC1D promoterDepositorInsertPinkyCaMP
UseAAVTagsExpressionMammalianMutationPromotergfaABC1DAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPMS-1852
Plasmid#232287PurposePhage DisplayDepositorInsertFn3 domain #10
UsePhage display vectorTagsPreScission Cleavage, HAExpressionMutationD7KPromoterAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFN35K SmBiT TK-neo Human collagen type IV alpha 3 chain (COL4A3)
Plasmid#229767PurposeHuman Col4A3 tagged at N-terminal with SmBiT to be used with N-tagged LgBiT hCol4A5 and Flag-tagged hCol4A4 in Nanobit assay systemDepositorInsertHuman collagen type IV alpha 3 chain (COL4A3) (COL4A3 Human)
UseLuciferaseTagsSmBiTExpressionMutationSee Depositor CommentsPromoterAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGL3_5UTR_Myc_FLuc
Plasmid#229502PurposeMyc 5' untranslated region firefly luciferase reporter.DepositorInsertMyc 5'UTR
UseTagsExpressionMammalianMutationPromoterSV40Available sinceFeb. 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCJ1021
Plasmid#228761PurposePlasmid expressing Cas9 and 2 gRNAs for mouse Ift88. Use for disruption of mouse Ift88 in cultured cells.DepositorInsertIft88 gRNAs (Ift88 Mouse)
UseCRISPRTags3XFLAG and GFPExpressionMammalianMutationPromoterhU6Available sinceFeb. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23[METUTRwt/luc2/minP]
Plasmid#226458Purposewt MET 5'UTR upstream FLucDepositorInsertMET 5'UTR
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23[uORF1/luc2/minP]
Plasmid#226459PurposeMET 5'UTR (uORF1 mutated) upstream FLucDepositorInsertMET 5'UTR uORF1 mutated
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23[uORF2/luc2/minP]
Plasmid#226460PurposeMET 5'UTR (uORF2 mutated) upstream FLucDepositorInsertMET 5'UTR uORF2 mutated
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23[uORF1,2/luc2/minP]
Plasmid#226461PurposeMET 5'UTR (uORF1 and uORF2 mutated) upstream FLucDepositorInsertMET 5'UTR uORF1 uORF2 mutated
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 7, 2025AvailabilityAcademic Institutions and Nonprofits only