We narrowed to 9,340 results for: UTY

1. pTcI38-Wasabi

   Plasmid

   #86117

   Purpose

   mWasabi reporter gene gated by temperature-sensitive Lambda repressor TcI38

   Depositor

   Mikhail Shapiro

   Article

   Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

   Inserts

   TcI38
   mWasabi
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   A67T, M1V, L65S, K68R, F115L, D126G, D188G

   Promoter

   pR/pL and pR/pL, LacI

   Available since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3-smURFP-StagRFP-EKAR-EV

   Plasmid

   #173019

   Purpose

   Red/far-red FRET-based ERK activity reporter for multiplexed imaging applications.

   Depositor

   Jin Zhang

   Article

   Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:

   Insert

   smURFP-StagRFP-EKAR-EV
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pENTR-D-TOPO-PAmCherry1-MCS

   Plasmid

   #60608

   Purpose

   Cloning PAmCherry1

   Depositor

   Steven Chu, Xiaolin Nan

   Article

   Nan et al Proc Natl Acad Sci U S A. 2013 Nov 12;11

   Insert

   PAmCherry1
   

   Use

   Cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. LbABE8e

   Plasmid

   #138504

   Purpose

   A-to-G base editor

   Depositor

   David Liu

   Article

   Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

   Insert

   ecTadA(8e)-dLbCas12a
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEGFP-C1-FLAG-Ku80

   Plasmid

   #46958

   Purpose

   Plasmid for expression of GFP-FLAG tagged human Ku80 (N-terminal tag). Confers resistance to G418.

   Depositor

   Steve Jackson

   Article

   Britton et al J Cell Biol. 2013 Jul 29.

   Insert

   Ku80 (XRCC5 Human)
   

   Use

   Tags

   GFP-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. ERM-APEX2

   Plasmid

   #79055

   Purpose

   Soybean APEX2 anchored to cytosolic face of ER membrane with N-terminal targeting sequence of residues 1-27 of ER-resident P450 oxidase 2C1.

   Depositor

   Alice Ting

   Article

   Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

   Insert

   APEX2 anchored to the cytosolic face of the ER membrane
   

   Use

   Lentiviral

   Tags

   Residues 1-27 of P450 oxidase 2C1 and V5

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV immearly promotor

   Available since

   Aug. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBT372

   Plasmid

   #125613

   Purpose

   Expresses evoCDA1-BE4max-NG in mammalian cells

   Depositor

   David Liu

   Article

   Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

   Insert

   evoCDA1-BE4max-NG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   F23S A123V I195F

   Promoter

   Available since

   July 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTECH-chPylRS(IPYE)

   Plasmid

   #99222

   Purpose

   Expression plasmid encoding chPylRS(IPYE) and pylT

   Depositor

   Dieter Söll

   Article

   Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

   Insert

   Plpp chPyl(IPYE)RS; PproK pylT
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Contains activating mutations V31I, T56P, H62Y, A…

   Promoter

   Plpp and PproK

   Available since

   Oct. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. p11-LacY-wtx1

   Plasmid

   #69056

   Purpose

   The reporter plasmid p11-LacY-wtx1 encodes the toxin CcdB gene under the inducible BAD promoter and a single copy of the endonuclease cleavage site which is readily extendable to multiple copies.

   Depositor

   Huimin Zhao

   Article

   Chen et al Nucleic Acids Res. 2005 Oct 6;33(18):e15

   Inserts

   ccdB (ccdB )
   lacY (lacY )
   

   Use

   Tags

   A177C

   Expression

   Mutation

   Promoter

   pBAD and pLac

   Available since

   Sept. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pPAmCherry2-C1

    Plasmid

    #31932

    Depositor

    Vladislav Verkhusha

    Article

    Subach et al Nat Methods. 2009 Feb;6(2):153-9. Epub 2

    Insert

    PAmCherry2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    M18L/K69N/L84F/A147T/S148L/I165L/Q167A/L169T/A179…

    Promoter

    CMV

    Available since

    Sept. 12, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pPPC009.106

    Plasmid

    #171142

    Purpose

    Expression of J106 scRNA on pBBR1-KmR plasmid

    Depositor

    Jesse Zalatan

    Article

    Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

    Insert

    J106 scRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23119

    Available since

    Oct. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMP31-1

    Plasmid

    #45905

    Depositor

    Harmit Malik

    Article

    Patel et al PLoS Biol. 2012;10(3):e1001282. doi: 10.

    Insert

    mitochondrial antiviral signaling protein (MAVS Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMSP1E3D1_D73C

    Plasmid

    #39328

    Depositor

    Stephen Sligar

    Article

    Ritchie et al Methods Enzymol. 2009 . 464():211-31.

    Insert

    MSP1E3D1_D73C (APOA1 synthetic gene)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    cys mutant of MSP1E3D1

    Promoter

    T7

    Available since

    Sept. 11, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pEGFP-C1-FLAG-Ku70

    Plasmid

    #46957

    Purpose

    Plasmid for cloning and epression of GFP-FLAG tagged human Ku70 (N-terminal tag). Confers resistance to G418.

    Depositor

    Steve Jackson

    Article

    Britton et al J Cell Biol. 2013 Jul 29.

    Insert

    Ku70 (XRCC6 Human)
    

    Use

    Tags

    GFP-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET-Bxb1-His

    Plasmid

    #123132

    Purpose

    Bacterial expression plasmid for wild-type Bxb1 with a C-terminal His-tag.

    Depositor

    David Liu

    Article

    Bessen et al Nat Commun. 2019 Apr 26;10(1):1937. doi:

    Insert

    Bxb1 integrase
    

    Use

    Tags

    6x His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLifeAct-mTurquoise2

    Plasmid

    #36201

    Purpose

    In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

    Insert

    LifeAct
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. S100A6

    Plasmid

    #196217

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses S100A6.

    Depositor

    Lalit Deshmukh

    Article

    Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

    Insert

    S100A6 (S100A6 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. AAV.CAG.GLPLight1

    Plasmid

    #187467

    Purpose

    Expresses GLPLight1 in mammalian cells

    Depositor

    Tommaso Patriarchi

    Article

    Duffet et al Elife. 2023 Jun 2;12:RP86628. doi: 10.75

    Insert

    GLPLight1
    

    Use

    AAV

    Tags

    Flag tag

    Expression

    Mutation

    Promoter

    CAG

    Available since

    Feb. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMN-334

    Plasmid

    #195468

    Purpose

    Lentiviral Integratable Inducible synZiFTR ZF10 + Reporter

    Depositor

    Ahmad Khalil

    Article

    Li et al Science. 2022 Dec 16;378(6625):1227-1234

    Insert

    inverted[8X ZF10 BS - ybTATA - mCherry] - pSFFV - ZF10 - NS3 - p65
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. human MMP-2 promoter

    Plasmid

    #53432

    Purpose

    promoter activity study

    Depositor

    Etty Benveniste

    Article

    Qin et al J Biol Chem. 1999 Oct 8;274(41):29130-7.

    Insert

    MMP2 promoter (MMP2 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    MMP2

    Available since

    June 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pMK462 (Hygro-P2A-mAB)

    Plasmid

    #214388

    Purpose

    mAID-BromoTag for N-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

    Insert

    Hygro-P2A-mAID-BromoTag
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Halo-MTS

    Plasmid

    #124315

    Purpose

    Expresses mitochondria specific HaloTag protein

    Depositor

    Jin Wang

    Article

    Jiang et al Antioxid Redox Signal. 2018 Oct 25. doi:

    Insert

    Halo tag
    

    Use

    Tags

    Mito specific seq

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pHAGE-TO-nls-st1dCas9-3nls-3XGFP

    Plasmid

    #64111

    Purpose

    Expression of St1 dCas9-3XGFP in mammalian cells

    Depositor

    Thoru Pederson

    Article

    Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    St1 dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3XsfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TO

    Available since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pNMT

    Plasmid

    #42578

    Depositor

    James Hurley

    Article

    Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 16;10

    Insert

    N-myristoyl tranferase (NMT1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    two silent mutations T690C and T942C

    Promoter

    T7

    Available since

    Aug. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

25. pBS-L1PA1-CH-mneo

    Plasmid

    #51288

    Purpose

    contains the fully codon optimized ORF1 and ORF2 of the human L1RP

    Depositor

    Astrid Roy-Engel

    Article

    Wagstaff et al PLoS One. 2011 May 10;6(5):e19672. doi:

    Insert

    L1 (L1RE1 Human)
    

    Use

    Tags

    mneoI indicator cassette containing an inverted n…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCMU-ERr

    Plasmid

    #61170

    Purpose

    Fluorescent reporter for endoplasmic reticulum AtWAK2sp-mCherry-HDEL expressed under AtUBQ10 promoter

    Depositor

    Maria Harrison

    Article

    Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

    Insert

    AtWAK2-mCherry-HDEL
    

    Use

    Tags

    mCherry

    Expression

    Plant

    Mutation

    Promoter

    AtUBQ10

    Available since

    April 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pBad-mCherry-XL (R125K)

    Plasmid

    #188054

    Purpose

    Expresses His-tagged, Arabinose-inducible mCherry-XL (R125K) in bacteria

    Depositor

    Ralph Jimenez

    Article

    Mukherjee et al J Phys Chem B. 2022 Jun 16. doi: 10.1021

    Insert

    mCherry-XL (R125K)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    differs from mCherry by R125K, W143S, I161V, Q163…

    Promoter

    araBAD

    Available since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pMVV108

    Plasmid

    #215338

    Purpose

    Expresses sfGFP under induction with the Salicylic Acid Marionette Cassette (NahRAM + PSalTTC-sfGFP) with a BBR1 backbone.

    Depositor

    Brian Pfleger

    Article

    Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

    Insert

    sfGFP
    

    Use

    Synthetic Biology

    Tags

    None

    Expression

    Bacterial

    Mutation

    Promoter

    PSalTTC

    Available since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pHR_PGK_antiHer24D5-7_synNotch_Gal4VP64

    Plasmid

    #85424

    Purpose

    Lentiviral vector for expression of anti-Her2 scFv 24D5-7 Gal4VP64 synthetic Notch

    Depositor

    Wendell Lim

    Article

    Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

    Insert

    anti-Her2_4D5-7_synNotch
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    PGK

    Available since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. CAG FUCCI

    Plasmid

    #175266

    Purpose

    Bicistronic FUCCI construct driven by a CAG promoter. Contains I-SceI sites for transgenesis

    Depositor

    James Monaghan

    Article

    Duerr et al Development. 2022 Mar 15;149(6). pii: 27

    Insert

    FUCCI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pKL003

    Plasmid

    #98919

    Purpose

    Constitutively expresses the voltage indicator PROPS under the Sigma 70 promoter in E. coli.

    Depositor

    Joel Kralj

    Article

    Bruni et al Proc Natl Acad Sci U S A. 2017 Aug 29;11

    Insert

    PROPS
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23118 - Strong Constitutive

    Available since

    Aug. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. 8xNFAT-ZsG-mCD8

    Plasmid

    #162747

    Purpose

    NFAT-driven ZsGreen-1 reporter gene with mouse CD8 gene

    Depositor

    Maki Nakayama

    Article

    Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

    Insert

    8x of NFAT binding motif followed by ZsGreen-1 fluorescent protein gene
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. mAzurite-16xRhoBAST

    Plasmid

    #196347

    Purpose

    Expression of mAzurite mRNA with 16xRhoBAST in 3'UTR

    Depositor

    Murat Sunbul

    Article

    Englert et al Nat Commun. 2023 Jun 30;14(1):3879. doi:

    Insert

    mAzurite-16xRhoBAST
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pET-His-Cre

    Plasmid

    #123128

    Purpose

    Bacterial expression plasmid for wild-type Cre with an N-terminal His-tag.

    Depositor

    David Liu

    Article

    Bessen et al Nat Commun. 2019 Apr 26;10(1):1937. doi:

    Insert

    Cre recombinase
    

    Use

    Tags

    6x His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAB1

    Plasmid

    #62547

    Purpose

    broad host range plasmid for IPTG-inducible expression of eGFP. Designed for imaging Pseudomonas aeruginosa activity in biofilms.

    Depositor

    Michael Franklin

    Article

    Walters MC et al Antimicrob Agents Chemother. 2003 Jan;47

    Insert

    eGFP
    

    Use

    Broad host range plasmid

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pTrc

    Available since

    April 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. Desmoglein2-mCherry

    Plasmid

    #36991

    Depositor

    Kathleen Green

    Article

    Nekrasova et al J Cell Biol. 2011 Dec 26;195(7):1185-203

    Insert

    Dsg2 (DSG2 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pMN-333

    Plasmid

    #195467

    Purpose

    Lentiviral Integratable Inducible synZiFTR ZF3 + Reporter

    Depositor

    Ahmad Khalil

    Article

    Li et al Science. 2022 Dec 16;378(6625):1227-1234

    Insert

    inverted[8X ZF3 BS - ybTATA - mCherry] - pSFFV - ZF3 - p65 - ERT2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pmTurquoise2-Peroxi

    Plasmid

    #36203

    Purpose

    In vivo visualization of the peroxisome (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

    Insert

    Peroxisomal targeting sequence
    

    Use

    Tags

    mTurquoise2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pRF0G-IodoY

    Plasmid

    #113645

    Purpose

    Plasmid contains the aminoacyl-tRNA synthetase and tRNA genes from methanococcus jannaschii for charging iodotyrosine at amber stop codons.

    Depositor

    Jeffrey Barrick

    Article

    Monk et al ACS Synth Biol. 2016 Sep 20.

    Insert

    IodoYRS and tRNA gene
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 27, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

40. pAR-Ec611

    Plasmid

    #130872

    Purpose

    A nuclear plasmid encoding an error-prone mutant TP-DNAP1 (I777K, L900S) for OrthoRep

    Depositor

    Chang Liu

    Article

    Ravikumar et al Cell. 2018 Dec 13;175(7):1946-1957.e13.

    Insert

    TP-DNAP1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    I777K, L900S

    Promoter

    Available since

    Aug. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pZZMP26

    Plasmid

    #231755

    Purpose

    Mammalian expression of TOP6BL

    Depositor

    Scott Keeney

    Article

    Zheng et al bioRxiv [Preprint]. 2024 Nov 21:2024.11.

    Insert

    TOP6BL (Gm960 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pMK445 (BSD-P2A-BromoTag)

    Plasmid

    #214375

    Purpose

    BromoTag for N-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

    Insert

    BSD-P2A-BromoTag
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. SaABE8e

    Plasmid

    #138500

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    ecTadA(8e)-nSaCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pEGFP-C1-TBP

    Plasmid

    #26674

    Depositor

    Sui Huang

    Article

    Chen et al Mol Biol Cell. 2002 Jan . 13(1):276-84.

    Insert

    TBP (TBP Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pFW1000

    Plasmid

    #224210

    Purpose

    E. coli-Bacteroides shuttle plasmid; pB8-51 (Bacteroides replication origin), R6K (E. coli replication origin)

    Depositor

    Ophelia Venturelli

    Article

    Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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46. p2T-CMV-evoCjCas9-TadCDd-BlastR

    Plasmid

    #202558

    Purpose

    Mammalian expression of evoCjCas9 TadCDd

    Depositor

    Gerald Schwank

    Article

    Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

    Insert

    evoCjCas9-TadCDd
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pBT13-splitD

    Plasmid

    #122597

    Purpose

    clone M13 phage by SapI Golden Gate

    Depositor

    David Liu

    Article

    Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

    Insert

    M13 bacteriophage genes
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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48. pEHlyA5

    Plasmid

    #168298

    Purpose

    Secretion of nanobodies and other polypeptides fused to C-terminal secretion signal of HlyA

    Depositor

    Luis Ángel Fernández

    Article

    Ruano-Gallego et al Microb Cell Fact. 2019 Mar 11;18(1):47.

    Insert

    N‑terminal His tag, VHH, HA and E‑tags, C‑HlyA
    

    Use

    Tags

    E-tag, HA-tag, His-tag, Prescission site, and VHH…

    Expression

    Bacterial

    Mutation

    Promoter

    LacI-Plac

    Available since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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49. pEGFP-C1-FLAG-XRCC4

    Plasmid

    #46959

    Purpose

    Plasmid for expression of GFP-FLAG tagged human XRCC4 isoform 2 (N-terminal tag). Confers resistance to G418.

    Depositor

    Steve Jackson

    Article

    Britton et al J Cell Biol. 2013 Jul 29.

    Insert

    XRCC4 (XRCC4 Human)
    

    Use

    Tags

    GFP-2xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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50. pmTurquoise2-C1

    Plasmid

    #60560

    Purpose

    Expresses the cyan fluorescent protein variant mTurquoise2, can be used to create fusion proteins

    Depositor

    Dorus Gadella

    Article

    Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

    Insert

    mTurquoise2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge