8,807 results

1. pCMV-PEmax-P2A-hMLH1dn

   Plasmid

   #174828

   Purpose

   Mammalian expression of SpCas9 PEmax prime editor with P2A-human MLH1dn (codon optimized)

   Depositor

   David Liu

   Article

   Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

   Insert

   PEmax-P2A-hMLH1dn
   

   Tags

   SV40 bpNLS and c-Myc NLS

   Expression

   Mammalian

   Mutation

   Detailed in manuscript; deletion of residues 754-…

   Promoter

   CMV

   Available Since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. HyPer7.2DAAO-NES

   Plasmid

   #168301

   Purpose

   Chemogenetic fluorescent H2O2 reporter targeted to the cytoplasm

   Depositor

   Thomas Michel

   Article

   Saeedi Saravi et al Redox Biol. 2020 Sep;36:101605. doi: 10.

   Insert

   HyPer7.2DAAO-NES
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pK036.TRE-Flpe-WPRE (Supernova)

   Plasmid

   #85007

   Purpose

   For Flpe/FRT-based Supernova, pK036 should be used with pK068 etc.

   Depositor

   Takuji Iwasato

   Article

   Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

   Insert

   NLS-FLPe-WPRE
   

   Expression

   Mammalian

   Available Since

   Nov. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEIGW roGFP2-Orp1

   Plasmid

   #64993

   Purpose

   Mammalian expression of cytosolic roGFP2-Orp1 (lentiviral vector)

   Depositor

   Tobias Dick

   Article

   Gutscher et al J Biol Chem. 2009 Nov 13;284(46):31532-4

   Insert

   Orp1 (HYR1 Budding Yeast)
   

   Use

   Lentiviral

   Tags

   roGFP2

   Expression

   Mammalian

   Available Since

   July 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAX APP Swe/Ind

   Plasmid

   #30145

   Depositor

   Dennis Selkoe, Tracy Young-Pearse

   Article

   Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

   Insert

   human APP 695 Swedish/Indiana mutation (APP Human)
   

   Expression

   Mammalian

   Mutation

   Swedish and Indiana; K595N, M596L and V642F

   Available Since

   Aug. 22, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA HA HP1 gamma

   Plasmid

   #24080

   Depositor

   Naoko Tanese

   Article

   Vassallo et al Proc Natl Acad Sci U S A. 2002 Apr 30. 9

   Insert

   HP1 gamma (CBX3 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Oct. 27, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAGM47523

   Plasmid

   #153221

   Purpose

   Level 0 zCas9i vector

   Depositor

   Sylvestre Marillonnet

   Article

   Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

   Insert

   SpCas9 with introns and 2 NLS
   

   Use

   Synthetic Biology

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. PIEZO1-HaloTag

   Plasmid

   #207834

   Purpose

   PIEZO1 fusion for biochemistry and live imaging

   Depositor

   Charles Cox

   Article

   Zhou et al Science. 2023 Aug 18;381(6659):799-804.

   Insert

   PIEZO1 (PIEZO1 Human)
   

   Tags

   HaloTag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti CMV/TO GFP-MDC1 (779-2)

   Plasmid

   #26285

   Depositor

   Eric Campeau

   Insert

   Mediator of DNA damage checkpoint 1 (MDC1 Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   Oct. 25, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1(+)/Luc2=tdT

    Plasmid

    #32904

    Purpose

    Expresses a fusion of firefly luciferase and tandem Tomato red fluorescent protein in mammalian cells.

    Depositor

    Christopher Contag

    Article

    Patel et al Cancer Res. 2010 Dec 15;70(24):10141-9.

    Inserts

    Firefly Luciferase
    tandem Tomato Red Fluorescent Protein
    

    Tags

    tdTomato red fluorescent protein

    Expression

    Mammalian

    Promoter

    CMV-IE and n/a

    Available Since

    Feb. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pgRNA-CKB

    Plasmid

    #73501

    Purpose

    gRNA expression vector (with mKate2)

    Depositor

    Bruce Conklin

    Article

    Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

    Insert

    mKate2-T2A-Bsd
    

    Use

    CRISPR and Lentiviral

    Tags

    NLS

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-CA

    Plasmid

    #69616

    Purpose

    To express gene strongly with AAV

    Depositor

    Naoshige Uchida

    Article

    Menegas et al Elife. 2015 Aug 31;4. doi: 10.7554/eLife

    Type

    Empty backbone

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Available Since

    Oct. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCW57.1-FOXM1c

    Plasmid

    #68810

    Purpose

    Doxycycline inducible lentiviral vector for FOXM1c expression in mammalian cells

    Depositor

    Adam Karpf

    Article

    Barger et al Oncotarget. 2015 Jul 16.

    Insert

    FOXM1c (FOXM1 Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    TRE promoter, Tet ON

    Available Since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. lenti-sgRNA puro

    Plasmid

    #104990

    Purpose

    This 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and puromycin resistance from an EF-1a promoter.

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pQCXIP-GFP1-10

    Plasmid

    #68715

    Purpose

    Split GFP assay

    Depositor

    Yutaka Hata

    Article

    Kodaka et al Exp Cell Res. 2015 Aug 15;336(2):171-81.

    Insert

    GFP1-10
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. Lck-mScarlet-I

    Plasmid

    #98821

    Purpose

    In vivo visualization of the plasma membrane (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

    Insert

    Lck
    

    Tags

    mScarlet-I

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. YFP-Parkin

    Plasmid

    #23955

    Purpose

    Mammalian expression of human Park2 fused to EYFP

    Depositor

    Richard Youle

    Article

    Narendra et al J Cell Biol. 2008 Dec 1. 183(5):795-803.

    Insert

    Park2 (PRKN Human)
    

    Tags

    eYFP

    Expression

    Mammalian

    Available Since

    April 9, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. 7M8

    Plasmid

    #64839

    Purpose

    This plasmid can be used in the triple transfection method of AAV vector production. It supplies the replication proteins from AAV2 and the 7M8 capsid protein.

    Depositor

    David Schaffer, John Flannery

    Article

    Dalkara et al Sci Transl Med. 2013 Jun 12;5(189):189ra

    Insert

    7M8 cap
    

    Use

    AAV

    Mutation

    7mer insertion in the AAV2 cap

    Available Since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. eSrtA(2A-9) pET29b

    Plasmid

    #75145

    Purpose

    reprogrammed sortase A variant that recognizes alternative substrate LAXTG with high activity and specificity

    Depositor

    David Liu

    Article

    Dorr et al Proc Natl Acad Sci U S A. 2014 Sep 16;11

    Insert

    reprogrammed sortase A variant
    

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    S102C A104H E105D K138P K152I N160K K162H T164N K…

    Promoter

    T7

    Available Since

    July 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. Luc-PS9

    Plasmid

    #177942

    Purpose

    Transient mammalian expression of Luc2 (firefly luciferase) along with SARS-CoV-2 sequence 20080-21171 (PS9) within the 3'UTR. Resulting transcript is packaged into SARS-CoV-2 virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    Luc2 (ORF1ab )
    

    Expression

    Mammalian

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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