We narrowed to 13,473 results for: Mpl

Showing: 2701 - 2750 of 13473 results

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pGEX-4T-3-SH3BP1

Plasmid

#136647

Purpose

Expression of SHBP1-GST fusion protein in E. coli

Depositor

Maria Balda, Karl Matter

Article

Elbediwy et al J Cell Biol. 2012 Aug 20;198(4):677-93.

Insert

SH3BP1 (SH3BP1 Human)

Expression

Bacterial

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-mCherry-ATG14

Plasmid

#203567

Purpose

Mammalian expression of mCherry tagged ATG14

Depositor

James Hurley

Article

Chen et al bioRxiv 2023.06.01.543278

Insert

ATG14 (ATG14 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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pJAT56

Plasmid

#204300

Purpose

Express phiC31 under control of D. melanoagaster vasa promoter, with Vasa 3’UTR.

Depositor

David Stern

Article

Stern et al bioRxiv 2023.06.17.545412

Insert

3XP3-EGFP

Use

CRISPR

Promoter

3XP3

Available Since

Aug. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pSplit-ON-hDib1

Plasmid

#58787

Purpose

Express hDib1-LucN fusion protein in mammalian cells

Depositor

Hiroyoshi Ariga

Article

Maita et al Anal Biochem. 2014 Feb 3. pii: S0003-269

Insert

hDib1 (TXNL4A Human)

Tags

Flag and Luciferase (1-398aa)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 15, 2014

Availability

Academic Institutions and Nonprofits only
pSTK120-mCMV-TK

Plasmid

#22054

Depositor

Maria Castro

Article

King et al J Virol. 2008 May . 82(9):4680-4.

Insert

mCMV-TK-pA expression cassette

Use

Adenoviral

Available Since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only
IVT-MCP-MMLV-RT

Plasmid

#207457

Purpose

in vitro transcription of mRNA

Depositor

Erik Sontheimer

Article

Liu et al Nat Biotechnol. 2023 Sep 14. doi: 10.103

Insert

MCP-RT

Expression

Bacterial

Available Since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pscALPS gfp Blasti

Plasmid

#115804

Purpose

Express gfp driven by SFFV promoter

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

gfp

Use

Lentiviral

Promoter

SFFV

Available Since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pDB4283

Plasmid

#98702

Purpose

A plasmid containing the 3' portion of the bsdMX marker and the gRNA elements downstream of the target sequence. It serves as the PCR template for generating the gRNA insert in the split-bsdMX system

Depositor

Li-Lin Du

Article

Zhang et al G3 (Bethesda). 2018 Apr 27. pii: g3.118.

Insert

gRNA PCR template

Use

CRISPR

Expression

Yeast

Available Since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pEF6 Vps53-V5-6His

Plasmid

#175097

Purpose

V5 tagged Vps53 is expressed in mammalian cells

Depositor

Juan Bonifacino

Article

Perez-Victoria et al Mol Biol Cell. 2008 Jun;19(6):2350-62. d

Insert

Vps53 (Vps53 Mouse)

Tags

V5

Expression

Mammalian

Available Since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-C3-Rabaptin-5

Plasmid

#199173

Purpose

Expresses GFP-Rabaptin-5 fusion in mammalian cells

Depositor

Juan Bonifacino

Article

Mattera et al EMBO J. 2003 Jan 2;22(1):78-88. doi: 10.

Insert

Rabaptin-5 (RABEP1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Contains Tyr 149 Ser substitution; insert starts …

Promoter

CMV

Available Since

April 18, 2023

Availability

Academic Institutions and Nonprofits only

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pKDR-CBh

Plasmid

#183557

Purpose

One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (non-attenuated CBh promoter)

Depositor

Erik Dent

Article

Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

Insert

Unattenuated

Expression

Mammalian

Promoter

CBh

Available Since

June 27, 2022

Availability

Academic Institutions and Nonprofits only

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pPMW-attB

Plasmid

#61814

Purpose

pUASp plasmid with attB for Drosophila transgenesis

Depositor

Frederique Peronnet

Article

Dardalhon-Cumenal et al PLoS Genet. 2018 Jul 11;14(7):e1007498.

Type

Empty backbone

Tags

6xMyc

Expression

Insect

Promoter

UASp

Available Since

March 17, 2015

Availability

Academic Institutions and Nonprofits only

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ESCRT-II

Plasmid

#17633

Depositor

James Hurley

Article

Hierro et al Nature. 2004 Sep 9. 431(7005):221-5.

Insert

pST39-Vps25(1-203) - His/Vps22(1-234) - Vps36 (1-567) (VPS36, SNF8, VPS25 Budding Yeast)

Expression

Bacterial

Available Since

March 28, 2008

Availability

Academic Institutions and Nonprofits only

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pRK5-Myc-TEAD4-Y429H

Plasmid

#33041

Depositor

Kunliang Guan

Article

Li et al Genes Dev. 2010 Feb 1;24(3):235-40.

Insert

TEAD4 (TEAD4 Human)

Tags

Myc

Expression

Mammalian

Mutation

Y429H

Available Since

March 28, 2012

Availability

Academic Institutions and Nonprofits only
hSDH5_G78R

Plasmid

#113047

Purpose

Expression of mutant hSDH5

Depositor

Edward A. Berry

Article

Zafreen et al Arch Biochem Biophys. 2016 Aug 15;604:47

Insert

hSDH5_G78R (SDHAF2 Human)

Expression

Bacterial

Mutation

G78R, Insert begins at amino acid 39

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pKDR-Lenti-TetOn

Plasmid

#183555

Purpose

pKDR compatible TetOn lentiviral transfer plasmid

Depositor

Erik Dent

Article

Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

Type

Empty backbone

Use

Lentiviral

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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3TERM_35S

Plasmid

#136114

Purpose

3TERM after CDS/ CDS12-CTAG

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

3TERM_35S (CaMV)

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pMAL728

Plasmid

#169152

Purpose

Encodes OptoQ-AMP6

Depositor

Jose Avalos

Article

Lalwani et al ACS Synth Biol. 2021 Aug 20;10(8):2060-2

Insert

VP16-EL222

Tags

SV40 NLS

Expression

Yeast

Mutation

A79Q

Available Since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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hSDH5_C83D

Plasmid

#113048

Purpose

Expression of mutant hSDH5

Depositor

Edward A. Berry

Article

Zafreen et al Arch Biochem Biophys. 2016 Aug 15;604:47

Insert

hSDH5_C83D (SDHAF2 Human)

Expression

Bacterial

Mutation

C83D, Insert begins at amino acid 39

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pJAT27

Plasmid

#204308

Purpose

For generating targeting inversions. C-terminal split GFP, expressed in flight muscles.

Depositor

David Stern

Article

Stern et al bioRxiv 2023.06.17.545412

Insert

MHC-GFPc

Use

CRISPR

Promoter

MHC

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pJAT18

Plasmid

#204307

Purpose

For generating targeting inversions. N-terminal split GFP, expressed in flight muscles.

Depositor

David Stern

Article

Stern et al bioRxiv 2023.06.17.545412

Insert

MHC-GFPn

Use

CRISPR

Promoter

MHC

Available Since

Aug. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pEB1-2xEGFP

Plasmid

#37827

Depositor

Torsten Wittmann

Article

Gierke et al Curr Biol. 2012 May 8;22(9):753-62. Epub

Insert

EB1 (MAPRE1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

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pEF6 Vps54-V5-6His

Plasmid

#175098

Purpose

V5 tagged Vps54 is expressed in mammalian cells

Depositor

Juan Bonifacino

Article

Perez-Victoria et al Mol Biol Cell. 2008 Jun;19(6):2350-62. d

Insert

Vps54 (Vps54 Mouse)

Tags

V5

Expression

Mammalian

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pDGE161

Plasmid

#79453

Purpose

recipient plasmid [multiplexing]; pPcUbi:Cas9, FAST, nptII

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

ccdB (ccdB(letD) )

Use

CRISPR

Expression

Plant

Mutation

BsaI site eliminated

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pscALPS_puro

Plasmid

#128504

Purpose

Encodes puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

Puromycin Resistance

Use

Lentiviral

Expression

Mammalian

Promoter

Cyclophilin A promoter

Available Since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

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LP376

Plasmid

#90200

Purpose

pCMV-myc with cDNA encoding HsTRAPPC10 aa residues 1000-1259

Depositor

Lotte Pedersen

Article

Schou et al Cilia. 2014 Jun 18;3:6. doi: 10.1186/204

Insert

TRAPPC10 (TRAPPC10 Human)

Tags

Myc

Expression

Mammalian

Mutation

aa residues 1000-1259

Promoter

CMV

Available Since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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pmCherry-p23

Plasmid

#108224

Purpose

Mammalian expression vector for mCherry fusion to human p23

Depositor

Didier Picard

Article

Picard et al Exp Cell Res. 2006 Nov 15. 312(19):3949-

Insert

mCherry-p23 (PTGES3 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 4, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-mTID-HA3-HIS3MX6

Plasmid

#227983

Purpose

To insert biotin ligase, miniTurboID, at the C-terminus of genes; yeast BioID,HIS3MX6 marker

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

miniTurboID

Tags

HA tag

Expression

Yeast

Available Since

Dec. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pJZC603

Plasmid

#62317

Purpose

sgRNA with 2x PP7 for yeast cells

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Insert

sgRNA + 2x PP7 RNA binding module

Expression

Yeast

Promoter

SNR52

Available Since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

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Atg24 1B

Plasmid

#197046

Purpose

His6 tagged Atg24 from S. cerevisiae for expression in E. coli.

Depositor

Michael Ragusa

Article

Reinhart et al Biochemistry. 2022 Apr 14. doi: 10.1021/

Insert

Atg24

Tags

6His-TEV

Expression

Bacterial

Available Since

July 19, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-ATG14

Plasmid

#203565

Purpose

Mammalian expression of ATG14

Depositor

James Hurley

Article

Chen et al bioRxiv 2023.06.01.543278

Insert

ATG14 (ATG14 Human)

Expression

Mammalian

Promoter

CMV

Available Since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-GST-ATG14

Plasmid

#203566

Purpose

Mammalian expression of GST tagged ATG14

Depositor

James Hurley

Article

Chen et al bioRxiv 2023.06.01.543278

Insert

ATG14 (ATG14 Human)

Tags

GST

Expression

Mammalian

Promoter

CMV

Available Since

July 13, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pFastBac TwinStrepII Prescission SNAPf TEV cloning vector with BioBrick polycistronic restriction sites (11-SNAP-V1)

Plasmid

#55216

Depositor

Scott Gradia

Article

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Tags

SNAPf and TwinStrepII

Expression

Insect

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRISPR_full-site

Plasmid

#186277

Purpose

Reporter plasmid for full-site selection assay

Depositor

Jennifer Doudna

Article

Wang et al Nat Commun. 2021 May 6;12(1):2571. doi:

Insert

Thiomicrospira leader repeat and modified chloramphenicol selection cassette (removed RBS and start codon))

Expression

Bacterial

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pTC201B

Plasmid

#52193

Purpose

for creating reversible targeting construct

Depositor

Tian Chi

Article

Chaiyachati et al BMC Biol. 2012 Nov 30;10:96. doi: 10.118

Insert

promoter-trap cassette Neo-Ires-GFP

Use

Cre/Lox

Tags

Neo, GFP

Available Since

April 23, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc3-14-3-3 Beta

Plasmid

#19957

Depositor

Yue Xiong

Article

Shumway et al J Biol Chem. 2003 Jan 24. 278(4):2089-92

Insert

14-3-3 Protein Beta (YWHAB Human)

Tags

Myc3

Expression

Mammalian

Available Since

April 1, 2009

Availability

Academic Institutions and Nonprofits only
pSUPER.PKD3.RNAi

Plasmid

#10818

Depositor

Alex Toker

Article

Hausser et al Nat Cell Biol. 2005 Sep . 7(9):880-6.

Insert

PKD3 RNAi (PRKD3 Human)

Use

RNAi

Expression

Mammalian

Available Since

Oct. 6, 2005

Availability

Academic Institutions and Nonprofits only
CDS_mALS

Plasmid

#136081

Purpose

Confers plant resistance to chlorsulfuron

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CDS_mALS

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pFA6a-mTID-HA3-KanMX6

Plasmid

#227982

Purpose

To insert biotin ligase, miniTurboID, at the C-terminus of genes; yeast BioID, KanMX6 marker

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

miniTurboID

Tags

HA tag

Expression

Yeast

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pTX1-RNA0

Plasmid

#126040

Purpose

In-vitro-transcription of control RNA0 in NMR tube for "Systems NMR" analysis

Depositor

Frédéric Allain

Article

Nikolaev et al Nat Methods. 2019 Aug;16(8):743-749. doi

Insert

RNA0

Available Since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pTYF-mCMV/GfaABC1D-EGFP

Plasmid

#19976

Depositor

Sergey Kasparov

Article

Liu et al BMC Biotechnol. 2008 May 16;8:49. doi: 1

Insert

mCMVGfaABC1D-EGFP

Expression

Mammalian

Available Since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only
pAct::Raf

Plasmid

#11341

Depositor

Michael Wigler

Article

Van Aelst et al Proc Natl Acad Sci U S A. 1993 Jul 1. 90

Insert

byr-2 (byr2 Fission Yeast)

Tags

Gal4 activation domain

Expression

Yeast

Available Since

May 25, 2006

Availability

Academic Institutions and Nonprofits only
pSLQ2853-3 pHR: U6-Sasgv2CXCR4-1 CMV-EGFP

Plasmid

#84254

Purpose

Expresses optimized Sa sgCXCR4-1 gRNA with an EGFP fluorescent marker

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Insert

Sa sgCXCR4-1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mouse U6

Available Since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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Ubiquitin I44A

Plasmid

#12648

Depositor

Rachel Klevit

Article

Brzovic et al Mol Cell. 2006 Mar 17. 21(6):873-80.

Insert

Ubiquitin (UBC Human)

Expression

Bacterial

Mutation

Ile44Ala

Available Since

July 31, 2007

Availability

Academic Institutions and Nonprofits only
cavin-3-HA

Plasmid

#27711

Depositor

Ari Helenius

Article

Hayer et al Traffic. 2010 Mar . 11(3):361-82.

Insert

cavin-3 (CAVIN3 Human)

Tags

HA

Expression

Mammalian

Mutation

R8P

Available Since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pJZC545

Plasmid

#62313

Purpose

sgRNA with 1x MS2 for yeast cells

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Insert

sgRNA + 1x MS2 binding module

Expression

Yeast

Promoter

SNR52

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pMJ915v2 + sfGFP

Plasmid

#88918

Purpose

For expression of modified SpyCas9 in e.coli.

Depositor

Jennifer Doudna

Article

Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Inserts

Cas9
T7

Use

CRISPR

Expression

Bacterial

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR-Rabin8

Plasmid

#24910

Depositor

Maxence Nachury

Article

Nachury et al Cell. 2007 Jun 15. 129(6):1201-13.

Insert

Rabin8 (RAB3IP Human)

Use

Gateway entry vector

Available Since

July 7, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pMP118

Plasmid

#207947

Purpose

GreenBraid destination vector pGBY1 - Eco31I - PaqCI -sfGFP- PaqCI - Eco31I

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Type

Empty backbone

Expression

Plant

Available Since

Nov. 17, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMaCTag-P31

Plasmid

#120042

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with V5

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

V5

Use

Pcr template

Tags

V5

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 13,473 results for: Mpl