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We narrowed to 12,628 results for: 109

Showing: 2281 - 2320 of 12628 results
  1. pcDNA4/TO-ORF66 C427A-2xStrep

    Plasmid
    #131109
    Purpose
    Expresses KSHV ORF66 a.a. 1-429 (full length) C427A mutant with a C-terminal 2xStrep tag in mammalian cells
    Depositor
    Insert
    ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
    Use
    Tags
    2xStrep
    Expression
    Mammalian
    Mutation
    ORF66 a.a. 1-429 (full-length); cysteine 427 muta…
    Promoter
    CMV
    Available since
    Oct. 17, 2019
    Availability
    Academic Institutions and Nonprofits only
  2. pFUSEss-CHIg-mG3_CH2charge

    Plasmid
    #105858
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgG3 antibody specific to antigen B of the ABO blood group system, clone M18. Introduced mutatnions modify charge of CH2 domain.
    Depositor
    Insert
    IgG3 heavy chain (Ighg3 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    mutated heavy chain of mouse IgG3, muatations: Hi…
    Promoter
    hEF1-HTLV prom
    Available since
    March 20, 2018
    Availability
    Academic Institutions and Nonprofits only
  3. pX458-Dual-Guide-Donor-ShuttleOnly

    Plasmid
    #171098
    Purpose
    Donor shuttle plasmid to enable ligation of a second U6-GuideRNA casssette into any pX458 family backbone via digestion of both plasmids with XbaI and KpnI.
    Depositor
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    XbaI site added upstream of U6 promotor. GtoC mu…
    Promoter
    Available since
    Aug. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
  4. pCasper4-g-osk-TRICK

    Plasmid
    #71244
    Purpose
    oskar translation biosensor with 12x copies of modified PP7 stem loops and 6xMS2 stem loops. . full genomic region, used for P element-mediated germline transformation in Drosophila.
    Depositor
    Insert
    oskar (osk Fly)
    Use
    P element-mediated germline transformation
    Tags
    12xPP7 and 6xMS2
    Expression
    Insect
    Mutation
    Promoter
    osk
    Available since
    Dec. 9, 2015
    Availability
    Academic Institutions and Nonprofits only
  5. pAAV2-CAG-3xNLS-vsfGFP-0

    Plasmid
    #191097
    Purpose
    To express a bright green nanobody FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methods
    Insert
    3xNLS-vsfGFP-0
    Use
    AAV
    Tags
    Green fluorescent protein bound to enhancer nanob…
    Expression
    Mammalian
    Mutation
    Optimized to Human codon usage
    Promoter
    CMV and CAG
    Available since
    Dec. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
  6. pCAGGS-RaichuEV-Rac1(Y40C)

    Plasmid
    #164903
    Purpose
    Expresses a Rac1 FRET sensor RaichuEV-Rac1 which contains a dominant negative mutation Y40C in the Rac1 domain. More useful than T17N mutation due to less harmful to endogenous signaling.
    Depositor
    Insert
    RaichuEV-Rac1 (RAC1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Y40C mutation in the Rac1 domain whcih make the s…
    Promoter
    Available since
    Aug. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
  7. pAAV2-CAG-3xNLS-mScarlet

    Plasmid
    #191098
    Purpose
    To express a bright monomeric red FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methods
    Insert
    3xNLS-mScarlet
    Use
    AAV and Synthetic Biology
    Tags
    Three repeats of the nuclear localizzation seque…
    Expression
    Mammalian
    Mutation
    Optimized to human codon usage
    Promoter
    CMV enhancer and CAG
    Available since
    Dec. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
  8. pTUB1-Sth.dCas9-P2A-CAT-T2A-TagBFP

    Plasmid
    #171090
    Purpose
    CRISPR interference. Construct for expressing catalytically inactive Cas9 (dCas9) from Streptococcus thermophilus (CRISPR1 locus) in Toxoplasma gondii. Suitable for generating stable cell lines.
    Depositor
    Inserts
    Sth1 dCas9
    Chloramphenicol acetyltransferase
    TagBFP
    Use
    CRISPR; Toxoplasma gondii expression
    Tags
    3xFLAG, P2A peptide, SV40 NLS, T2A peptide, and n…
    Expression
    Mutation
    D9A, H599A
    Promoter
    TUB1 (Toxoplasma gondii), transcriptionally linke…
    Available since
    Oct. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
  9. pDONR221-SLC10A4_STOP

    Plasmid
    #161109
    Purpose
    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.
    Insert
    SLC10A4 (SLC10A4 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 21, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
  10. pAAV2-CAG-3xNLS-AausFP1

    Plasmid
    #191096
    Purpose
    To express a bright green FP to label eucaryotic cell nuclei. To be used in tissue-clearing methods and other fluorescent microscopy methods
    Insert
    3xNLS-AausFP1
    Use
    AAV and Synthetic Biology
    Tags
    Three repeats of the nuclear localizzation seque…
    Expression
    Mammalian
    Mutation
    Optimized to Human codon usage
    Promoter
    CMV enhancer and CAG
    Available since
    Dec. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
  11. pFUSEss-CHIg-mG3_CH3-1

    Plasmid
    #105859
    Purpose
    Expression plasmid coding for hybrid heavy chain of mouse IgG3 antibody specific to antigen B of the ABO blood group system, clone M18. The antibody comprises IgG1-derived CH3.
    Depositor
    Insert
    IgG1, IgG3 (Ighg1, Ighg3 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    hybrid heavy chain of mouse IgG3 with CH3 derived…
    Promoter
    hEF1-HTLV prom
    Available since
    March 20, 2018
    Availability
    Academic Institutions and Nonprofits only
  12. pFUSEss-CHIg-mG3_CH2-1

    Plasmid
    #105857
    Purpose
    Expression plasmid coding for hybrid heavy chain of mouse IgG3 antibody specific to antigen B of the ABO blood group system, clone M18. The antibody comprises IgG1-derived CH2.
    Depositor
    Insert
    IgG1, IgG3 (Ighg1, Ighg3 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    hybrid heavy chain of mouse IgG3 with CH2 derived…
    Promoter
    hEF1-HTLV prom
    Available since
    March 20, 2018
    Availability
    Academic Institutions and Nonprofits only
  13. pFUSEss-CHIg-mG3_CH1h-1

    Plasmid
    #105856
    Purpose
    Expression plasmid coding for hybrid heavy chain of mouse IgG3 antibody specific to antigen B of the ABO blood group system, clone M18. The antibody comprises IgG1-derived CH1 and hinge.
    Depositor
    Insert
    IgG1, IgG3 (Ighg1, Ighg3 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    hybrid heavy chain of mouse IgG3 with CH1 and hin…
    Promoter
    hEF1-HTLV prom
    Available since
    March 20, 2018
    Availability
    Academic Institutions and Nonprofits only
  14. pFUSEss-CHIg-mG3_h-1

    Plasmid
    #105861
    Purpose
    Expression plasmid coding for hybrid heavy chain of mouse IgG3 antibody specific to antigen B of the ABO blood group system, clone M18. The antibody comprises IgG1-derived hinge.
    Depositor
    Insert
    IgG1, IgG3 (Ighg1, Ighg3 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    hybrid heavy chain of mouse IgG3 with hinge deriv…
    Promoter
    hEF1-HTLV prom
    Available since
    March 20, 2018
    Availability
    Academic Institutions and Nonprofits only
  15. pYPQ223-STU

    Plasmid
    #138109
    Purpose
    Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized, catalytically deactivated AsCas12a-SRDX repressor without promoter and terminator
    Depositor
    Insert
    dAsCas12a-SRDX
    Use
    CRISPR; Gateway compatible cas12a entry clone
    Tags
    NLS
    Expression
    Plant
    Mutation
    Promoter
    Available since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
Showing: 2281 - 2320 of 12628 results