Skip to main content
Addgene
Showing: 251 - 300 of 2950 results

  2. pAPV-DsRed2-FLAG-ER-5

    Plasmid
    #170622
    Purpose
    Expresses DsRed2-FLAG targeted to the ER lumen with an N-terminal APV tripeptide after signal peptide cleavage. The protein is efficiently retrieved due to exposure of KDEL.
    Depositor
    Ke Xu
    Article
    Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.
    Insert
    APV-DsRed2-ER-5
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    170622-plasmid-map-sequence-id-335778

    Enlarge

  11. CaMKII-Aion-ts-citrine-ER

    Plasmid
    #192576
    Purpose
    AAV-vector for optogenetic long-term silencing of excitatory neurons
    Depositor
    Simon Wiegert
    Insert
    Aion
    Use
    AAV
    Tags
    ts-Citrine-ER
    Expression
    Mammalian
    Mutation
    T59S, E83N, E90Q, E101S, V117R, E123S, C128A,D156…
    Promoter
    CaMKIIa(0.4)
    Available since
    Dec. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    192576-plasmid-map-sequence-id-379301

    Enlarge

  12. pAPV-FLAG-DsRed2-ER-5

    Plasmid
    #170620
    Purpose
    Expresses FLAG-DsRed2 targeted to the ER lumen, but the encoded protein escapes from the ER into tubular ERGICs due to the N-terminal APV tripeptide after SP cleavage.
    Depositor
    Ke Xu
    Article
    Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.
    Insert
    APV-FLAG-DsRed2-ER-5
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    170620-plasmid-map-sequence-id-335780

    Enlarge

  14. pAPV-mEmerald-DsRed2-ER-5

    Plasmid
    #170681
    Purpose
    Expresses mEmerald-DsRed2 targeted to the ER lumen, but the encoded protein escapes from the ER into tubular ERGICs due to the N-terminal APV and poorly exposed KDEL.
    Depositor
    Ke Xu
    Article
    Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.
    Insert
    APV-mEmerald-DsRed2-ER-5
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    170681-plasmid-map-sequence-id-335805

    Enlarge

  15. pAPV-DsRed2-mEmerald-ER-5

    Plasmid
    #170715
    Purpose
    Expresses DsRed2-mEmerald targeted to the ER lumen. The encoded protein is effectively retrieved back to the ER due to exposed KDEL despite the N-terminal APV.
    Depositor
    Ke Xu
    Article
    Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.
    Insert
    APV-DsRed2-mEmerald-ER-5
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    170715-plasmid-map-sequence-id-335807

    Enlarge

  16. pMRX-IP-EGFP-PA-BAX(ER)

    Plasmid
    #228941
    Purpose
    Stable expression of EGFP-LOV2(N538E)-BAX-Cyb5a in mammalian cells. Photoactivatable BAX localizes on ER and induces ER rupture upon blue light stimulation.
    Depositor
    Noboru Mizushima
    Article
    Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.
    Inserts
    LOV2 domain of phototropin1
    BAX (BAX Human)
    Cyb5a (Cyb5a Rat)
    Use
    Retroviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    N-terminus and C-terminus are deleted. BAX(3-171)…
    Promoter
    Available since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228941-plasmid-map-sequence-id-469413

    Enlarge

  17. pcDNA3/er-(n2)oxStayGold(c4)v2.0

    Plasmid
    #186296
    Purpose
    A new version of er-(n2)oxStayGold(c4), which gives a higher expression level. The StayGold gene has codon optimization for mammalian expression. For brighter ER imaging if permitted.
    Depositor
    Atsushi Miyawaki
    Article
    Hirano et al Nat Biotechnol. 2022 Apr 25. pii: 10.103
    Insert
    er-(n2)oxStayGold(c4)v2.0
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    186296-plasmid-map-sequence-id-366145

    Enlarge

  23. p663-UBC-ER-TurboID-V5_IDG-K

    Plasmid
    #214453
    Purpose
    Gateway destination clone of ER-TurboID (endoplasmic reticulum control) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    ER-TurboID-V5
    Use
    Lentiviral; Gateway destination
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    March 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    214453-plasmid-map-sequence-id-425197

    Enlarge

  25. p426MET25_SSKar2-sfpHluorin-HDEL (ER-sfpHluorin, MRV66)

    Plasmid
    #115698
    Purpose
    ER-targeted superfolder pHluorin for improved pH measurements in the ER of S. cerevisiae
    Depositor
    Eckhard Boles
    Article
    Reifenrath et al Sci Rep. 2018 Aug 10;8(1):11985. doi: 10
    Insert
    SSKar2-sfpHluorin-HDEL
    Use
    Tags
    ER retention signal containing HDEL and ER signal…
    Expression
    Yeast
    Mutation
    for introduced mutations see publication
    Promoter
    MET25
    Available since
    Sept. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    115698-plasmid-map-sequence-id-223642

    Enlarge

  29. pStr-KDEL_APV-SBP-DsRed2-ER-5

    Plasmid
    #170675
    Purpose
    Expresses ER-targeted streptavidin-KDEL (hook) and SBP-DsRed2 targeted to the ER lumen with an N-terminal APV tripeptide (cargo) for RUSH.
    Depositor
    Ke Xu
    Article
    Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.
    Inserts
    APV-SBP-DsRed2-ER-5
    streptavidin-KDEL
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    IRES and T7
    Available since
    Jan. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    170675-plasmid-map-sequence-id-335803

    Enlarge

  33. pStr-KDEL_EPV-SBP-DsRed2-ER-5

    Plasmid
    #170676
    Purpose
    Expresses ER-targeted streptavidin-KDEL (hook) and SBP-DsRed2 targeted to the ER lumen with an N-terminal EPV tripeptide (cargo) for RUSH.
    Depositor
    Ke Xu
    Article
    Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.
    Inserts
    EPV-SBP-DsRed2-ER-5
    streptavidin-KDEL
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    IRES and T7
    Available since
    Jan. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    170676-plasmid-map-sequence-id-335802

    Enlarge

  35. pAAV hSyn INTRON mEmerald-ER WPRE

    Plasmid
    #236230
    Purpose
    AAV expression of a fluorescent marker, mEmerald fused to Prolactin signal peptide and KDEL sequence; for expression and retention in the lumen of the endoplasmic reticulum
    Depositor
    Jennifer Lippincott-Schwartz
    Article
    Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do
    Insert
    mEmerald-PRL signal peptide-KDEL sequence (PRL Bovine)
    Use
    AAV
    Tags
    mEmerald
    Expression
    Mutation
    Promoter
    human Synapsin 1
    Available since
    May 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    236230-plasmid-map-sequence-id-471247

    Enlarge

  38. p663-UBC-ER-miniTurbo-V5_IDG-K

    Plasmid
    #214452
    Purpose
    Gateway destination clone of ER-miniTurbo (endoplasmic reticulum control) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    ER-miniTurbo-V5
    Use
    Lentiviral; Gateway destination
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    214452-plasmid-map-sequence-id-423209

    Enlarge

  39. p406 – TDH3-ER-FlucDM-GFP11-Ura

    Plasmid
    #177731
    Purpose
    DM Firefly luciferase reporter fused to a KAR2 signal sequence for ER targeting and HDEL for ER retention with an HA tag and the eleventh β-strand of GFP added to the C-terminus in yeast plasmid with
    Depositor
    Erik Böttger
    Article
    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:
    Insert
    FlucDM
    Use
    Tags
    GFP11, HA, HDEL, and KAR2 Signal Sequence
    Expression
    Bacterial and Yeast
    Mutation
    R188Q, R261Q (Destabilized Mutant)
    Promoter
    Available since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    177731-plasmid-map-sequence-id-352614

    Enlarge

  40. p406 – TDH3-ER-FlucDM-GFP- Ura

    Plasmid
    #177732
    Purpose
    DM Firefly luciferase reporter fused to a KAR2 signal sequence for ER targeting and HDEL for ER retention and fused to eGFP at the C-terminus in yeast plasmid with URA marker
    Depositor
    Erik Böttger
    Article
    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:
    Insert
    FlucDM
    Use
    Tags
    HA, HDEL, KAR2 Signal Sequence, and eGFP
    Expression
    Bacterial and Yeast
    Mutation
    R188Q, R261Q (Destabilized Mutant)
    Promoter
    Available since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    177732-plasmid-map-sequence-id-352622

    Enlarge

  41. pStr-KDEL_APV-SBP-Dendra2-ER-5

    Plasmid
    #170679
    Purpose
    Expresses ER-targeted streptavidin-KDEL (hook) and SBP-Dendra2 targeted to the ER lumen with an N-terminal APV tripeptide (cargo) for RUSH.
    Depositor
    Ke Xu
    Article
    Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.
    Inserts
    APV-SBP-Dendra2-ER-5
    streptavidin-KDEL
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    IRES and T7
    Available since
    Jan. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    170679-plasmid-map-sequence-id-335798

    Enlarge

  47. L0-T12 GG2-ER-tagBFP-tUBQ10-GG3

    Plasmid
    #219695
    Purpose
    Level 0 to construct history-dependent target
    Depositor
    Jennifer Nemhauser
    Insert
    GG2-ER-tagBFP-tUBQ10-GG3
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    219695-plasmid-map-sequence-id-435727

    Enlarge

Showing: 251 - 300 of 2950 results