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We narrowed to 18,247 results for: multi

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Showing: 1181 - 1200 of 18247 results

  1. eGFPbait-E2A-KalTA4-pA donor vector

    Plasmid
    #61069
    Purpose
    for CRISPR/Cas9 mediated insertion of E2A-KalTA4; to be used in combination with a eGFP specific sgRNA (e.g. Plasmid 61051)
    Depositor
    Filippo Del Bene
    Publication
    Auer et al Genome Res. 2014 Jan;24(1):142-53. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    eGFPbait
    KalTA4
    Use
    Zebrafish expression
    Tags
    E2A
    Available since
    Feb. 12, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    61069-plasmid-map-sequence-id-99003

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  3. pMaster12

    Plasmid
    #58527
    Purpose
    Reprogram somatic cells to iPS cells
    Depositor
    Mario Capecchi
    Publication
    Wu et al Proc Natl Acad Sci U S A. 2014 Jul 22;11
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    neo
    tk
    oct4 (POU5F1 Human)
    Klf4 (KLF4 Human)
    Sox2 (SOX2 Human)
    Myc (MYC Human)
    EBNA-1
    Amp
    Lin28 (LIN28A Human)
    NANOG (NANOG Human)
    IRES
    NR5A2 (NR5A2 Human)
    miR302/367 (MIR367, MIR302A Human)
    Expression
    Mammalian
    Available since
    Feb. 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    58527-plasmid-map-sequence-id-138420

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  4. pSLQ2818 pPB: CAG-PYL1-KRAB-IRES-Puro-WPRE-SV40PA PGK-ABI-tagBFP-SpdCas9

    Plasmid
    #84241
    Purpose
    Expresses ABA-inducible KRAB-Sp dCas9
    Depositor
    Stanley Qi
    Publication
    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    ABI-tagBFP-Sp dCas9
    PYL1-KRAB
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    2xNLS (SV40), ABI, HA tag, IRES-Puro, PYL1, and t…
    Expression
    Mammalian
    Promoter
    CAG and PGK
    Available since
    Nov. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    84241-plasmid-map-sequence-id-160882

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  6. TRUPATH Triple Gaq

    Plasmid
    #196058
    Purpose
    Encodes a G alpha subunit (GNAQ) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Publication
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    GAlphaQ-RLuc8 (GNAQ Human)
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    196058-plasmid-map-sequence-id-385868

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  8. pSLQ10844

    Plasmid
    #183956
    Purpose
    CAG-hyperdCas12a-HA-miniVPR-mCherry-T2A-HygroR
    Depositor
    Stanley Qi
    Publication
    Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HyperdCas12a
    Use
    CRISPR
    Tags
    HA, Hygromycin Resistance, and mCherry
    Expression
    Mammalian
    Mutation
    D832A, D156R, E292R, D235R, D350R
    Promoter
    CAG
    Available since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    183956-plasmid-map-sequence-id-363081

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  10. pJZC43

    Plasmid
    #66565
    Purpose
    sgRNA + 2XPP7 with PCP-VP64 effector for mammalian cells, marked by mCherry
    Depositor
    Wendell Lim, Stanley Qi
    Publication
    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    sgRNA + 2XPP7
    PCP-VP64 IRES mCherry
    Expression
    Mammalian
    Promoter
    CMV and U6
    Available since
    July 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    66565-plasmid-map-sequence-id-113829

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  14. pmCherry-C1 actin-3XNLS P2A mCherry

    Plasmid
    #58475
    Purpose
    Expresses nuclear-targeted human actin, with an mCherry expression reporter after a P2A protease cleavage site, on a CMV promoter
    Depositor
    Dyche Mullins
    Publication
    Belin et al Elife. 2015 Aug 19;4. doi: 10.7554/eLife
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    beta actin (ACTB Human)
    3XNLS
    P2A cleavage site
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    58475-plasmid-map-sequence-id-90385

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  15. pU6-(BbsI)_CBh-Cas9-T2A-mCherry-H1-(BamHI)

    Plasmid
    #64217
    Purpose
    Expression vector for sgRNAs cloned into the BbsI sites, shRNAs into BamHI & AflII and for Expression of Cas9 linked to mCherry via T2A
    Depositor
    Ralf Kuehn
    Publication
    Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Cas9
    hU6 promoter; BbsI sites for sgRNA
    H1 promoter; BamHI site for shRNA
    Use
    CRISPR
    Tags
    3xFLAG, NLS, and T2A-mCherry
    Expression
    Mammalian
    Promoter
    CBh, H1, and U6
    Available since
    Sept. 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    64217-plasmid-map-sequence-id-123057

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  16. pC0053-CMV-dPspCas13b-GS-ADAR2DD(E488Q)-delta-984-1090

    Plasmid
    #103869
    Purpose
    dPspCas13b-ADAR2DD(E488Q) fusion that can be used to selectively edit adenosine to inosine in RNA molecules when used in conjuction with a guide RNA. Cas13b is truncated to allow for expression by AAV
    Depositor
    Feng Zhang
    Publication
    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    PspCas13b
    huADAR2DD (ADARB1 Human)
    Use
    CRISPR
    Tags
    GS and HIV NES
    Expression
    Mammalian
    Mutation
    Changed histidne 133 to alanine and histidine 105…
    Available since
    Nov. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    103869-plasmid-map-sequence-id-199177

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  17. pMasterK

    Plasmid
    #165081
    Purpose
    Episomal expression of reprogramming factors.
    Depositor
    Hideyuki Okano
    Publication
    Yoshimatsu et al Stem Cell Res. 2021 May;53:102380. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    OCT4 (POU5F1 Human)
    SOX2 (SOX2 Human)
    KLF4 (KLF4 Human)
    MYC (MYC Human)
    LRH1 (NR5A2 Human)
    NANOG (NANOG Human)
    lin28 (LIN28A Human)
    EGFP
    HSV TK
    KDM4D (KDM4D Callithrix jacchus)
    GLIS1 (GLIS1 Human)
    Use
    Episomal
    Expression
    Mammalian
    Available since
    April 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    165081-plasmid-map-sequence-id-514763

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  20. pAAV.hSynap.(cyto)iGlucoSnFR-mRuby2

    Plasmid
    #164510
    Purpose
    Green glucose sensor with mRuby2 fusion, cytosolic. The depositing lab recommends the updated pAAV.hSynap.(cyto).iGlucoSnFR2.mRuby3 (Plasmid #244092) instead of this plasmid.
    Depositor
    Loren Looger
    Publication
    Keller et al Cell Rep. 2021 Jun 22;35(12):109284. doi
    Has service
    AAV9
    Insert
    (Cyto)iGlucoSnFR-mRuby2
    Use
    AAV
    Mutation
    Synthetic Construct
    Promoter
    Synapsin
    Available since
    Feb. 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    164510-plasmid-map-sequence-id-323856

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Showing: 1181 - 1200 of 18247 results