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mVenus-C1-GEFT

Plasmid

#84340

Purpose

Expresses p63 (474 a.a.) tagged with mVenus

Depositor

Dorus Gadella

Article

van Unen et al Sci Rep. 2016 Nov 11;6:36825. doi: 10.10

Insert

GEFT (ARHGEF25 Synthetic, Human)

Tags

mVenus

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pET21a-hAIM2-PYD-GST

Plasmid

#51531

Purpose

Express GST-tagged PYD of hAIM2 in bacteria

Depositor

Emad Alnemri

Article

Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

Insert

AIM2 (AIM2 Human)

Tags

GST and T7

Expression

Bacterial

Mutation

deletion of amino acids 102-343

Promoter

lac operator

Available Since

March 11, 2014

Availability

Academic Institutions and Nonprofits only

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Lenti-FAB(ER-LD)

Plasmid

#214420

Purpose

Detection of ER-lipid droplet contact site

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

RspAN-FRB-V5-ER_IRES_CFAST-20nm-Halo-6xHp

Use

Lentiviral

Tags

CFAST-Halo and RspAN-FRB-V5

Promoter

CMV

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pBAD-T-GECO1

Plasmid

#191616

Purpose

Bacterial expression plasmids for Teal Fluorescent Genetically Encoded Calcium Indicator T-GECO1

Depositor

Robert Campbell

Article

Aggarwal et al Neurophotonics. 2024 Apr;11(2):024207. d

Insert

T-GECO1

Tags

His tag

Expression

Bacterial

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pSin_EF1a_tagBFP_P2A_NLS_pMag_CreC60_IRES_PuroR

Plasmid

#176311

Purpose

Expresses NLS-pMag-CreC60 with tagBFP marker

Depositor

Yingxiao Wang

Article

Allen et al ACS Synth Biol. 2019 Oct 18;8(10):2359-2

Insert

tagBFP_P2A_NLS_pMag_CreC60_IRES_PuroR

Use

Lentiviral and Synthetic Biology

Tags

tagBFP

Expression

Mammalian

Promoter

human EF1alpha

Available Since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pSin_EF1a_ERT2_CreN59_nMag_P2A_mCh_IRES_PuroR

Plasmid

#192636

Purpose

Expresses ERT2-CreN59-nMag-NLS with mCherry marker.

Depositor

Yingxiao Wang

Article

Allen et al ACS Synth Biol. 2019 Oct 18;8(10):2359-2

Insert

ERT2_CreN59_nMag_NLS_P2A_mCh_IRES_PuroR

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

EF1alpha

Available Since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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LV_EF1a_FLAG-Ikkbeta(S177E,S181E)-P2A-Hygro_Barcode

Plasmid

#170226

Purpose

Barcoded lentiviral vector to express Ikkbeta (S177E, S181E) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

Depositor

Prashant Mali

Article

Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

Insert

IKBKB (IKBKB Human)

Use

Lentiviral

Tags

FLAG

Mutation

S177E, S181E

Promoter

EF1a

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-MtAcKRS (human-opti)

Plasmid

#164195

Purpose

To express a Methanosarcina thermophila derived AcKRS in mammalian cells for genetic code expansion

Depositor

Tao Liu

Article

Hu et al ACS Synth Biol. 2020 Oct 16;9(10):2723-2

Insert

MtAcKRS-478(human-opti)

Use

Synthetic Biology

Expression

Mammalian

Mutation

D76G; L325M; L329I; Y330F; L333A; C372F

Promoter

CMV

Available Since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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MTK1_008

Plasmid

#123671

Purpose

Encodes ConLS and chicken hypersensitivity site 4 insulator as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector LS-2xHS4

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MiniCoopR U6:gRNA spred1, mitfa:Cas9

Plasmid

#118843

Purpose

Targets spred1 and expresses zebrafish mitfa specifically in zebrafish melanocytes

Depositor

Leonard Zon

Article

Ablain et al Science. 2018 Nov 1. pii: science.aau650

Insert

spred1 gRNA (spred1 Zebrafish)

Use

CRISPR; Tol2

Available Since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 FLAG mTOR 1968-2549 S2035T

Plasmid

#23000

Depositor

Jing Huang

Article

Lomenick et al Proc Natl Acad Sci U S A. 2009 Dec 22;10

Insert

mTOR (MTOR Human)

Tags

FLAG

Expression

Mammalian

Mutation

S2035T aa 1968-2549 only

Available Since

May 18, 2010

Availability

Academic Institutions and Nonprofits only
pFastBac-rAPOBEC1-pAG-hADAR2dd

Plasmid

#248100

Purpose

Expresses rAPOBEC1-pAG-hADAR2dd in insect cells

Depositor

Yangming Wang

Article

Cheng et al Nat Methods. 2025 Sep;22(9):1824-1835. d

Inserts

rAPOBEC1 (Apobec1 Rat)
protein A/G
hADAR2dd (ADARB1 Human)

Tags

His and Strep

Expression

Insect

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pDual-IN-SMN1

Plasmid

#215721

Purpose

dual-color reporter express eGFP constitutively ; tdTomato expression is contingent on the inclusion of the frame-shifting cassette exon 7; expresses SMN1

Depositor

Chaolin Zhang

Article

Recinos et al Nat Commun. 2024 May 7;15(1):3839. doi:

Insert

SMN1 EXON 7 (SMN1 Human)

Expression

Mammalian

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-SpyCatcher

Plasmid

#223633

Purpose

Protein purification of SpyCatcher003 (S49C) with 6xHis tag from E. coli

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

SpyCatcher003 S49C

Tags

His6

Expression

Bacterial

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetOminiCMV-Oct4-P2A-Klf4-IRES-Sox2-17

Plasmid

#210020

Purpose

tetO-OKS* (Dox-inducible reprogramming lentiviral vector expressing mouse Oct4 + Klf4 + super-Sox)

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

Oct4-p2a-Klf4-IRES-Sox2-17 (Klf4, Pou5f1, Sox2 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

TetO-mini-CMV

Available Since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_nV5-AMOT (p130)

Plasmid

#172997

Purpose

constitutive expression of V5-tagged AMOT (p130)

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

V5-AMOT (p130) (AMOT Human)

Tags

V5

Expression

Mammalian

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a_CnCas12f1

Plasmid

#204996

Purpose

CnCas12f1 heterologous expression plasmid

Depositor

Quanjiang Ji

Article

Su et al Nat Chem Biol. 2024 Feb;20(2):180-189. d

Insert

CnCas12f1

Use

CRISPR

Promoter

T7

Available Since

Aug. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pGL4.15_pSOX17

Plasmid

#195496

Purpose

Firefly luciferase promoter expression vector driven only by the promoter element of human SOX17

Depositor

Alexander Meissner

Article

Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

Insert

Promoter region of SOX17 (SOX17 Human)

Tags

luc2-hPEST

Expression

Mammalian

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pMXs-hSOX2-17

Plasmid

#193350

Purpose

Retroviral vector expressing human SOX2-17 chimeric transcription factor (super-SOX)

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

SOX2-17 (SOX2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

SOX2-17 is engineered highly cooperative chimeric…

Promoter

5'LTR

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pCXLE-SOX2-p2a-KLF4

Plasmid

#193292

Purpose

episomal hSK (expressing human SOX2 + KLF4)

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

SOX2-p2a-KLF4 (SOX2, KLF4 Human)

Expression

Mammalian

Mutation

F2A of original pCXLE-SK vector was replaced with…

Promoter

CAG

Available Since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-3*Flag-APEX2 N-term COILIN

Plasmid

#187581

Purpose

Express FLAG epitope and APEX2-tagged COILIN fusion protein in mammalian cells

Depositor

Benjamin Blencowe

Article

Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

Insert

COILIN (COIL Human)

Tags

APEX2-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-Ubiquitin-K27R-K29R

Plasmid

#121156

Purpose

Mammalian expression of HA tagged ubiquitin K27R and K29R

Depositor

Josef Kittler

Article

Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

Insert

Ubiquitin C (UBC Human)

Expression

Mammalian

Mutation

K27 and K29 mutated to Rs

Available Since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_KLF13_P2A_Hygro_Barcode

Plasmid

#120499

Purpose

Barcoded lentiviral vector to express KLF13 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

KLF13 (KLF13 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_KLF7_P2A_Hygro_Barcode

Plasmid

#120493

Purpose

Barcoded lentiviral vector to express KLF7 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

KLF7 (KLF7 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a_SOX3_P2A_Hygro_Barcode

Plasmid

#120481

Purpose

Barcoded lentiviral vector to express SOX3 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

SOX3 (SOX3 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a_NEUROG1_P2A_Hygro_Barcode

Plasmid

#120467

Purpose

Barcoded lentiviral vector to express NEUROG1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

NEUROG1 (NEUROG1 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a_NEUROG3_P2A_Hygro_Barcode

Plasmid

#120468

Purpose

Barcoded lentiviral vector to express NEUROG3 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

NEUROG3 (NEUROG3 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a_HOXA11_P2A_Hygro_Barcode

Plasmid

#120453

Purpose

Barcoded lentiviral vector to express HOXA11 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

HOXA11 (HOXA11 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-mAID-BE3

Plasmid

#113420

Purpose

Expresses mAID-BE3 in mammalian cells

Depositor

Jia Chen

Article

Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

Insert

mAID-BE3 (Aicda S. pyogenes and Bacteriophage PBS2, Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV_pMBP-dnVamp2-P2AT2A-EGFP-caax

Plasmid

#190153

Purpose

Expresses dominant-negative Vamp2 (mouse Vamp2 AA 1-94) plus membrane-targeted EGFP in oligodendrocytes; AAV vector

Depositor

Brad Zuchero

Article

Lam et al Nat Commun. 2022 Sep 23;13(1):5583. doi:

Insert

Vamp2 (Vamp2 Mouse)

Use

AAV

Tags

P2AT2A-EGFP-caax

Expression

Mammalian

Mutation

Truncation that includes only AA #1-94

Promoter

MBP

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
lentiCRISPR v2-sgDHODH-2

Plasmid

#186023

Purpose

knock out DHODH in mammalian cells

Depositor

Boyi Gan

Article

Mao et al Nature. 2021 May;593(7860):586-590. doi:

Insert

Dihydroorotate Dehydrogenase (Quinone) (DHODH Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
lentiCRISPR v2-sgACSL4

Plasmid

#186024

Purpose

knock out ACSL4 in mammalian cells

Depositor

Boyi Gan

Article

Mao et al Nature. 2021 May;593(7860):586-590. doi:

Insert

Acyl-CoA Synthetase Long Chain Family Member 4 (ACSL4 Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL4.27_GRHL-SPE

Plasmid

#177774

Purpose

Luciferase reporter for GRHL

Depositor

Michael Ristow

Article

Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

Insert

GRHL

Use

Luciferase

Available Since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
LV_EF1a_CCNB1-P2A-Hygro_Barcode

Plasmid

#170218

Purpose

Barcoded lentiviral vector to express CCNB1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

Depositor

Prashant Mali

Article

Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

Insert

CCNB1 (CCNB1 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
MTK1_023

Plasmid

#123685

Purpose

Encodes ConL1 and human B1 insulator as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector L1- B1 insulator

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
MTK1_024

Plasmid

#123686

Purpose

Encodes ConL2 and human C3 insulator as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector L2-C3 insulator

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
MTK2_001

Plasmid

#123697

Purpose

Encodes pTRE as a Type 2 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pTRE

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
MTK2_006

Plasmid

#123701

Purpose

Encodes pSV40 as a Type 2 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pSV40

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
KA1396_pGL4-UBCmin-luc2/Klf4-E'

Plasmid

#124187

Purpose

Luciferase reporter driven by the Klf4 enhancer

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Insert

Klf4 enhancer

Use

Luciferase

Promoter

UBCmin

Available Since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
P16ΔS:S10-(MCS)-3xHA

Plasmid

#108183

Purpose

Binary vectors used for the constitutive expression of tripartite split-sfGFP components (with a 3xHA tag) in plants

Depositor

Tzu-Yin Liu

Article

Liu et al Plant J. 2018 Feb 16. doi: 10.1111/tpj.1

Type

Empty backbone

Use

Synthetic Biology

Expression

Plant

Promoter

P16ΔS

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 6,639 results for: WUS;