4,168 results

1. pHR-SFFV-KRAB-dCas9-P2A-mCherry

   Plasmid

   #60954

   Purpose

   2nd Generation Lentiviral vector. Expresses an N-terminal KRAB-dCas9 fusion protein and mCherry

   Depositor

   Jonathan Weissman

   Article

   Gilbert et al Cell. 2014 Oct 23;159(3):647-61. doi: 10

   Insert

   KRAB-dCas9-P2A-mCherry fusion
   

   Use

   CRISPR and Lentiviral

   Tags

   2x NLS, HA, KRAB domain, and mCherry

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   Jan. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. rAAV2/MG1.2

   Plasmid

   #184541

   Purpose

   AAV packaging plasmid, expressing Rep2 and AAV-MG1.2 capsid

   Depositor

   Minmin Luo

   Article

   Lin et al Nat Methods. 2022 Jul 25. pii: 10.1038/s

   Insert

   AAV-MG1.2 capsid
   

   Use

   AAV; Helper vector for packaging

   Mutation

   changes on AAV9 capsid: K449R, Q588T; insertion o…

   Available Since

   July 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-PGK-Neo-PIP-FUCCI

   Plasmid

   #118616

   Purpose

   Lentiviral expression of the cell cycle marker PIP-FUCCI

   Depositor

   Jean Cook

   Article

   Grant et al Cell Cycle. 2018;17(21-22):2496-2516. do

   Insert

   PIP-FUCCI
   

   Use

   Lentiviral

   Tags

   mCherry and mVenus

   Expression

   Mammalian

   Promoter

   hPGK

   Available Since

   May 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pXR002: EF1a-dCasRx-2A-EGFP

   Plasmid

   #109050

   Purpose

   Catalytically inactive dCasRx with 2A-EGFP

   Depositor

   Patrick Hsu

   Article

   Konermann et al Cell. 2018 Mar 8. pii: S0092-8674(18)302

   Insert

   dCasRx
   

   Use

   CRISPR and Lentiviral

   Tags

   2A-eGFP, HA, and NLS

   Expression

   Mammalian

   Mutation

   R239A/H244A/R858A/H863A

   Available Since

   April 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP-C1-ADRP

   Plasmid

   #87161

   Purpose

   Fluorescent protein localized to lipid droplets (LDs)

   Depositor

   Elina Ikonen

   Article

   Salo et al EMBO J. 2016 Dec 15;35(24):2699-2716. Ep

   Insert

   ADRP (PLIN2 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. mEmerald-Clathrin-15

   Plasmid

   #54040

   Purpose

   Localization: Clathrin Vesicles, Excitation: 487, Emission: 509

   Depositor

   Michael Davidson

   Article

   Fiolka et al Proc Natl Acad Sci U S A. 2012 Apr 3;109

   Insert

   Clathrin (CLTB Human)
   

   Tags

   mEmerald

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 2, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pDisplay-pHuji

   Plasmid

   #61556

   Purpose

   Red fluorescent protein based pH indicator in pDisplay vector

   Depositor

   Robert Campbell

   Article

   Shen et al J Cell Biol. 2014 Nov 10;207(3):419-32.

   Insert

   pHuji
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 19, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pscAAV-CAG-GFP

   Plasmid

   #83279

   Purpose

   recombinant AAV vector packaging self-complementary GFP under the CAG promoter

   Depositor

   Mark Kay

   Article

   Pekrun et al JCI Insight. 2019 Nov 14;4(22). pii: 131

   Insert

   enhanced green fluorescent protein
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP-N1 alpha-actinin 1

   Plasmid

   #11908

   Depositor

   Carol Otey

   Article

   Edlund et al Cell Motil Cytoskeleton. 2001 Mar . 48(3

   Insert

   alpha-actinin 1 (ACTN1 Human)
   

   Tags

   eGFP

   Expression

   Mammalian

   Available Since

   Aug. 7, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBS-L1PA1-CH-mneo

    Plasmid

    #51288

    Purpose

    contains the fully codon optimized ORF1 and ORF2 of the human L1RP

    Depositor

    Astrid Roy-Engel

    Article

    Wagstaff et al PLoS One. 2011 May 10;6(5):e19672. doi:

    Insert

    L1 (L1RE1 Human)
    

    Tags

    mneoI indicator cassette containing an inverted n…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. Raptor_1 shRNA

    Plasmid

    #1857

    Depositor

    David Sabatini

    Article

    Sarbassov et al Science 2005 Feb 18;307(5712):1098-101.

    Insert

    Raptor (RPTOR Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    April 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSLQ1651-sgRNA(F+E)-sgGal4

    Plasmid

    #100549

    Purpose

    sgGal4-expressing vector modified from Addgene plasmid #51024

    Depositor

    Jian Xu

    Article

    Liu et al Cell. 2017 Aug 24;170(5):1028-1043.e19.

    Insert

    sgGal4
    

    Use

    CRISPR

    Tags

    sgRNA

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-HA-JMJD2B

    Plasmid

    #24181

    Purpose

    Mammalian expression of human JMJD2B with HA tag

    Depositor

    Kristian Helin

    Article

    Cloos et al Nature. 2006 Jul 20. 442(7100):307-11.

    Insert

    JMJD2B (KDM4B Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Jan. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. pML107

    Plasmid

    #67639

    Purpose

    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains LEU2 marker for yeast transformation

    Depositor

    John Wyrick

    Article

    Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1

    Inserts

    Cas9
    single guide RNA expression cassette
    

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    pSNR52 and pTDH3 (aka GAP promoter)

    Available Since

    Aug. 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pROSA26-PA

    Plasmid

    #21271

    Depositor

    Frank Costantini

    Article

    Srinivas et al BMC Dev Biol. 2001;1:4. doi: 10.1186/147

    Insert

    ROSA26 (Gt(ROSA)26Sor Mouse)
    

    Use

    Mouse Targeting

    Available Since

    Oct. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV2/rh10

    Plasmid

    #112866

    Purpose

    AAV packaging plasmid expressing Rep/Cap genes

    Depositor

    James M. Wilson

    Insert

    Rep2/Cap-rh10
    

    Use

    AAV

    Promoter

    Truncated P5

    Available Since

    Sept. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAKTaq

    Plasmid

    #25712

    Purpose

    Bacterial expression of Taq polymerase

    Depositor

    David Engelke

    Article

    Engelke et al Anal Biochem. 1990 Dec . 191(2):396-400.

    Insert

    Taq polymerase
    

    Expression

    Bacterial

    Available Since

    June 24, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. pC0052-REPAIR non-targeting guide clone into pC0043

    Plasmid

    #103868

    Purpose

    Non-targeting guide RNA for use with PspCas13b. Useful as a control for RNA editing and RNA knockdown experiments.

    Depositor

    Feng Zhang

    Article

    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

    Insert

    non-targeting guide RNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Dec. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUC57-TPNOX

    Plasmid

    #87853

    Purpose

    This plasmid contains human codon optimized sequence of Lactobacillus brevis mutant of a water-forming oxidase that can be used to increase NADP+/NADPH ratio in mammalian cells.

    Depositor

    Vamsi Mootha

    Article

    Cracan et al Nat Chem Biol. 2017 Aug 7. doi: 10.1038/

    Insert

    TPNOX
    

    Tags

    C-terminal FLAG tag with a linker

    Expression

    Bacterial

    Available Since

    Aug. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKLV2-U6gRNA5(BbsI)-PGKpuro2AmCherry-W

    Plasmid

    #67977

    Purpose

    CRISPR gRNA expression vector with an improved scaffold and puro/mCherry markers

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette, PGKpuro2AmCherry cassette, WPRE
    

    Use

    Lentiviral

    Mutation

    Deleted BbsI site within WPRE

    Promoter

    human U6 promoter, mouse PGK promoter

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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