4,133 results

1. pSRK2706

   Plasmid

   #78571

   Purpose

   Expresses untagged human rhinovirus 3C protease in E. coli

   Depositor

   David Waugh

   Article

   Raran-Kurussi et al Anal Biochem. 2016 Apr 19;504:30-37. doi

   Insert

   human rhinovirus 3C protease (HRVBgp1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. PPRE X3-TK-luc

   Plasmid

   #1015

   Purpose

   PPAR reporter. 3xDR1 sites upstream of a luciferase reporter.

   Depositor

   Bruce Spiegelman

   Article

   Kim et al Proc Natl Acad Sci U S A 1998 Apr 14;95(

   Insert

   PPRE
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 3, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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3. LRG2.1

   Plasmid

   #108098

   Purpose

   Lentiviral expression plasmid of sgRNA with GFP

   Depositor

   Christopher Vakoc

   Article

   Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter for sgRNA expression and EFS promoter…

   Available since

   June 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP-C1-Fibrillarin

   Plasmid

   #26673

   Depositor

   Sui Huang

   Article

   Chen et al J Cell Biol. 2001 Apr 2. 153(1):169-76.

   Insert

   Fibrillarin (FBL Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 10, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a-LIC

   Plasmid

   #26094

   Purpose

   SGC Empty backbone for bacterial expression

   Depositor

   Cheryl Arrowsmith

   Type

   Empty backbone

   Use

   Tags

   6xHis and Thrombin cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7-lacO (lactose/IPTG inducible)

   Available since

   Aug. 20, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pLenti CMV Neo DEST (705-1)

   Plasmid

   #17392

   Purpose

   3rd gen lentiviral Gateway destination vector, expression, CMV promoter, Neo

   Depositor

   Eric Campeau, Paul Kaufman

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral; Destination vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. mCherry-TFR-20

   Plasmid

   #55144

   Purpose

   Localization: Transferrin Receptor, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   TFR (TFRC Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1(+)-GRP78/BiP

   Plasmid

   #32701

   Depositor

   Richard C. Austin

   Article

   Werstuck et al J Clin Invest. 2001 May;107(10):1263-73.

   Insert

   GRP78/BiP (HSPA5 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAVS1-NDi-CRISPRi (Gen2)

   Plasmid

   #73498

   Purpose

   Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus

   Depositor

   Bruce Conklin

   Article

   Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

   Inserts

   dCas9-KRAB
   rtTA
   

   Use

   CRISPR

   Tags

   HA, KRAB, and NLS

   Expression

   Mammalian

   Mutation

   D10A, H840A (catalytically deactivated Cas9)

   Promoter

   CAG and TRE3G

   Available since

   Dec. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. mCerulean3-N1

    Plasmid

    #54730

    Purpose

    Localization: N1 Cloning Vector, Excitation: 433, Emission: 475

    Depositor

    Michael Davidson

    Article

    Markwardt et al PLoS One. 2011 . 6(3):e17896.

    Type

    Empty backbone

    Use

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. ppyCAG_RNaseH1_D210N

    Plasmid

    #111904

    Purpose

    Express D210N mutated RNASEH1 which abolishes the catalytic activity of RNASEH1

    Depositor

    Xiang-Dong Fu

    Article

    Chen et al Mol Cell. 2017 Nov 16;68(4):745-757.e5.

    Insert

    RNASEH1 (RNASEH1 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Changed D 210 to N

    Promoter

    Available since

    July 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. 4XCLEAR-luciferase reporter

    Plasmid

    #66800

    Purpose

    Reporter for activity of the CLEAR network

    Depositor

    Albert La Spada

    Article

    Cortes et al Nat Neurosci. 2014 Sep;17(9):1180-9. doi

    Insert

    4XCLEAR
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Four CLEAR elements (GTCACGTGAC) in tandem derive…

    Available since

    Nov. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. lentiCRISPR - EGFP sgRNA 1

    Plasmid

    #51760

    Purpose

    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

    Depositor

    Feng Zhang

    Article

    Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:

    Inserts

    Cas9
    Puromycin resistance
    EGFP sgRNA 1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS and U6

    Available since

    March 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-Bxb1

    Plasmid

    #182142

    Purpose

    Expression of human codon-optimized Bxb1

    Depositor

    David Liu

    Article

    Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

    Insert

    human codon optimized Bxb1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMSCV-hygro-STING

    Plasmid

    #102598

    Purpose

    Express STING for IFN-beta luciferase reporter assay.

    Depositor

    Nicolas Manel

    Article

    Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

    Insert

    STING (STING1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. HA-CYLD-WT

    Plasmid

    #15506

    Depositor

    Stephen Elledge

    Article

    Stegmeier et al Proc Natl Acad Sci U S A. 2007 May 22. 1

    Insert

    CYLD (CYLD Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 13, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. HA-DDX3

    Plasmid

    #44975

    Depositor

    Robin Reed

    Article

    Lee et al Nucleic Acids Res. 2008 Aug;36(14):4708-

    Insert

    DDX3 (DDX3X Human)
    

    Use

    Tags

    HA and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMA3790

    Plasmid

    #70110

    Purpose

    mtDNA elimination/production of rho-0 cells

    Depositor

    Mikhail Alexeyev

    Article

    Spadafora et al PLoS One. 2016 May 2;11(5):e0154684. doi

    Inserts

    EGFP
    hUNG1 Y147A
    Neo
    

    Use

    Tags

    None, human OTC mitochondrial targeting sequence,…

    Expression

    Mammalian

    Mutation

    Y147A, aa's 1-77 removed

    Promoter

    CMV, EF1a, and SV40 and native

    Available since

    May 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pmTurquoise2-Golgi

    Plasmid

    #36205

    Purpose

    In vivo visualization of golgi apparatus (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

    Insert

    Golgi targeting sequence (B4GALT1 Human)
    

    Use

    Tags

    mTurquoise2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUC18R6KT-mini-Tn7T-Km

    Plasmid

    #64969

    Purpose

    mini-Tn7 base vector with transcriptional terminator and oriT

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Methods. 2005 Jun;2(6):443-8.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 2, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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