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Showing: 3151 - 3200 of 37659 results

  2. pENTR-EUI

    Plasmid
    #111835
    Purpose
    Entry vector for adenoviral expression of Ecdysone receptor-based inducible transgene expression switch.
    Depositor
    Hyunju Ro
    Article
    Lee et al Mol Ther Nucleic Acids. 2016 Sep 27;5(9)
    Insert
    GAL4-minimal VP16-EcR (EcR Synthetic)
    Use
    Entry vector for expression
    Tags
    Expression
    Mutation
    Promoter
    CMV, 10XUAS
    Available since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    111835-plasmid-map-sequence-id-218214

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  6. pSN840

    Plasmid
    #184832
    Purpose
    Expresses human KIF5A(∆exon27) and human KLC1 in insect cells
    Depositor
    Kyoko Chiba
    Article
    Nakano et al Genes Cells. 2022 Apr 17. doi: 10.1111/g
    Inserts
    KIF5A (∆exon27) (KIF5A Human)
    KLC1 (KLC1 Human)
    Use
    Cre/Lox
    Tags
    His-FLAG and mScarlet-2xStrepII
    Expression
    Insect
    Mutation
    ∆exon27 form of human KIF5A
    Promoter
    p10 and polyhedrin
    Available since
    July 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    184832-plasmid-map-sequence-id-367218

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  12. PB-TO-hNIL

    Plasmid
    #172113
    Purpose
    Piggybac Tet-ON plasmid for differentiating hiPSCs into lower motor neurons via NGN2, ISL1, and LHX3 expression
    Depositor
    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward
    Article
    Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1
    Inserts
    hNGN2 (NEUROG2 Human)
    hISL1 (ISL1 Human)
    hLHX3 (LHX3 Human)
    puroR-T2A-mycNLS-mTagBFP2
    Use
    Tags
    T2A-mycNLS-mTagBFP2
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a and TRE3G
    Available since
    April 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    172113-plasmid-map-sequence-id-359519

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  14. pAB1677 REPAIR.t3

    Plasmid
    #176320
    Purpose
    CMV-HIVNES-GS-dCas13bt3- (GGS)2-huADAR2dd(E488Q) (REPAIR.t3 expression)
    Depositor
    Feng Zhang
    Article
    Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103
    Inserts
    human codon optimized Cas13bt3 (catalytically inactivated)
    huADAR2dd(E488Q) (ADARB1 Human)
    Use
    CRISPR
    Tags
    HIV NES
    Expression
    Mammalian
    Mutation
    E488Q, only the deaminase domain (aa 276-702 are …
    Promoter
    CMV
    Available since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    176320-plasmid-map-sequence-id-345287

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  15. pAB1923 REPAIR.t1-S

    Plasmid
    #176321
    Purpose
    CMV-HIVNES-GS-dCas13bt1- (GGS)2- huADAR2dd(E488Q/E620G/Q696L) (REPAIR.t1-S expression, specificity enhancing mutations)
    Depositor
    Feng Zhang
    Article
    Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103
    Inserts
    human codon optimized Cas13bt1 (catalytically inactivated)
    huADAR2dd(E488Q) (ADARB1 Human)
    Use
    CRISPR
    Tags
    HIV NES
    Expression
    Mammalian
    Mutation
    E488Q/E620G/Q696L, only the deaminase domain (aa …
    Promoter
    CMV
    Available since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    176321-plasmid-map-sequence-id-345288

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  18. pAB1676 REPAIR.t1

    Plasmid
    #176319
    Purpose
    CMV-HIVNES-GS-dCas13bt1- (GGS)2-huADAR2dd(E488Q) (REPAIR.t1 expression)
    Depositor
    Feng Zhang
    Article
    Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103
    Inserts
    human codon optimized Cas13bt1 (catalytically inactivated)
    huADAR2dd(E488Q) (ADARB1 Human)
    Use
    CRISPR
    Tags
    HIV NES
    Expression
    Mammalian
    Mutation
    E488Q, only the deaminase domain (aa 276-702 are …
    Promoter
    CMV
    Available since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    176319-plasmid-map-sequence-id-345279

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  26. 3xFLAG-DGKδ2-ΔSAMD

    Plasmid
    #226507
    Purpose
    Express N-terminal three tandem FLAG epitope tags, followed by an enterokinase cleavage site and human DGKD gene deleted SAM domain
    Depositor
    Chiaki Murakami, Fumio Sakane
    Article
    Murakami et al J Biol Chem. 2020 Mar 6;295(10):2932-294
    Insert
    DGKD (DGKD Human)
    Use
    Tags
    Three tandem FLAG epitope tag and enterokinase cl…
    Expression
    Mammalian
    Mutation
    deleted sterile alpha motif domain (deleted amino…
    Promoter
    CMV
    Available since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    226507-plasmid-map-sequence-id-457149

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  29. pGGG-Sr43

    Plasmid
    #186974
    Purpose
    Sr43 is a wheat stem rust resistance gene transferred from tall wheat grass (Thinopyrum ponticum) into bread wheat (Triticum aestivum).
    Depositor
    Brande Wulff
    Article
    Yu et al Nat Genet. 2023 Jun;55(6):921-926. doi:
    Insert
    Sr43
    Use
    Tags
    None
    Expression
    Bacterial and Plant
    Mutation
    None
    Promoter
    Natice
    Available since
    April 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    186974-plasmid-map-sequence-id-382711

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  30. pAB2076 pAAV REPAIR.t1

    Plasmid
    #176323
    Purpose
    pAAV EFS-HIVNES-dCas13bt1- (GGS)2-huADAR2(E488Q)-3xHA BGHpolyA::U6-BpiI-Cas13bt1 DR (full REPAIR.t1 + crRNA expression for AAV production)
    Depositor
    Feng Zhang
    Article
    Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103
    Inserts
    human codon optimized Cas13bt1 (catalytically inactivated)
    huADAR2dd(E488Q) (ADARB1 Human)
    Cas13bt1 crRNA + BpiI cloning site
    Use
    AAV and CRISPR
    Tags
    3xHA and HIV NES
    Expression
    Mammalian
    Mutation
    E488Q, only the deaminase domain (aa 276-702 are …
    Promoter
    EFS (short EF1alpha) and hU6
    Available since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    176323-plasmid-map-sequence-id-345286

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  32. pAdeasy-CMV-AE-EF1α-R2

    Plasmid
    #194928
    Purpose
    expressing two fusion proteins of Ag85B-ESAT6 and Rv2031c-Rv2626c from multi-stage antigens of Mycobacterium tuberculosis
    Depositor
    Limei Wang
    Article
    Wang et al Vaccines (Basel). 2024 Sep 6;12(9):1022.
    Inserts
    AE
    EEF1A1 (EEF1A1 Human)
    R2
    Use
    Adenoviral
    Tags
    Ag85B-ESAT6, EF1a, and Rv2031c-Rv2626c
    Expression
    Mutation
    a 48 bp linker (Gly3ser)4 was added between the t…
    Promoter
    CMV and EF1a
    Available since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    194928-plasmid-map-sequence-id-391107

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  33. pPB-C6F/TdTomato

    Plasmid
    #140826
    Purpose
    OCT4, SOX2, KLF4, C-MYC, KLF2, NANOG (C6F) and tdTomato under the control of TRE (Tet-ON)
    Depositor
    Hideyuki Okano
    Article
    Kisa et al Stem Cell Reports. 2017 Dec 12;9(6):1825
    Inserts
    OCT4 (POU5F1 Human)
    SOX2 (SOX2 Human)
    KLF4 (KLF4 Human)
    C-MYC (MYC Human)
    KLF2 (KLF2 Human)
    NANOG (NANOG Human)
    tdTomato
    Use
    Tags
    Expression
    Mammalian
    Mutation
    L104P- please see depositor comments
    Promoter
    Available since
    Sept. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    140826-plasmid-map-sequence-id-307041

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  34. kiCAP-AAV-MyoAAV4A

    Plasmid
    #224448
    Purpose
    Rep/Cap plasmid for the production of MyoAAV 4A, a muscle-tropic AAV capsid in mice and macaques.
    Depositor
    Pardis Sabeti
    Article
    Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.
    Insert
    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    Use
    AAV
    Tags
    Expression
    Mutation
    RGDYNSL insert between amino acids 588 and 589 of…
    Promoter
    p41
    Available since
    Sept. 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    224448-plasmid-map-sequence-id-446642

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  35. kiCAP-AAV-MyoAAV4E

    Plasmid
    #224452
    Purpose
    Rep/Cap plasmid for the production of MyoAAV 4E, a muscle-tropic AAV capsid in mice and macaques.
    Depositor
    Pardis Sabeti
    Article
    Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.
    Insert
    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    Use
    AAV
    Tags
    Expression
    Mutation
    RGDFNNT insert between amino acids 588 and 589 of…
    Promoter
    p41
    Available since
    Sept. 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    224452-plasmid-map-sequence-id-446621

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  36. kiCAP-AAV-MyoAAV1A

    Plasmid
    #224437
    Purpose
    Rep/Cap plasmid for the production of MyoAAV 1A, a muscle-tropic AAV capsid in mice.
    Depositor
    Pardis Sabeti
    Article
    Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.
    Insert
    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    Use
    AAV
    Tags
    Expression
    Mutation
    RGDLTTP insert between amino acids 588 and 589 of…
    Promoter
    p41
    Available since
    Sept. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    224437-plasmid-map-sequence-id-446668

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  37. kiCAP-AAV-MyoAAV2A

    Plasmid
    #224440
    Purpose
    Rep/Cap plasmid for the production of MyoAAV 2A, a muscle-tropic AAV capsid in mice.
    Depositor
    Pardis Sabeti
    Article
    Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.
    Insert
    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    Use
    AAV
    Tags
    Expression
    Mutation
    RGDQTTL insert between amino acids 588 and 589 of…
    Promoter
    p41
    Available since
    Sept. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    224440-plasmid-map-sequence-id-446665

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  38. kiCAP-AAV-MyoAAV1E

    Plasmid
    #224439
    Purpose
    Rep/Cap plasmid for the production of MyoAAV 1E, a muscle-tropic AAV capsid in mice.
    Depositor
    Pardis Sabeti
    Article
    Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.
    Insert
    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    Use
    AAV
    Tags
    Expression
    Mutation
    RGDTMSK insert between amino acids 588 and 589 of…
    Promoter
    p41
    Available since
    Sept. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    224439-plasmid-map-sequence-id-446666

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  39. kiCAP-AAV-MyoAAV3A

    Plasmid
    #224442
    Purpose
    Rep/Cap plasmid for the production of MyoAAV 3A, a muscle-tropic AAV capsid in mice and macaques.
    Depositor
    Pardis Sabeti
    Article
    Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.
    Insert
    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    Use
    AAV
    Tags
    Expression
    Mutation
    RGDYVGL insert between amino acids 588 and 589 of…
    Promoter
    p41
    Available since
    Sept. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    224442-plasmid-map-sequence-id-446662

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  40. kiCAP-AAV-MyoAAV3F

    Plasmid
    #224447
    Purpose
    Rep/Cap plasmid for the production of MyoAAV 3F, a muscle-tropic AAV capsid in mice and macaques.
    Depositor
    Pardis Sabeti
    Article
    Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.
    Insert
    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    Use
    AAV
    Tags
    Expression
    Mutation
    RGDHASW insert between amino acids 588 and 589 of…
    Promoter
    p41
    Available since
    Sept. 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    224447-plasmid-map-sequence-id-446643

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  41. kiCAP-AAV-MyoAAV1C

    Plasmid
    #224438
    Purpose
    Rep/Cap plasmid for the production of MyoAAV 1C, a muscle-tropic AAV capsid in mice and macaques.
    Depositor
    Pardis Sabeti
    Article
    Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.
    Insert
    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    Use
    AAV
    Tags
    Expression
    Mutation
    RGDLSTP insert between amino acids 588 and 589 of…
    Promoter
    p41
    Available since
    Sept. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    224438-plasmid-map-sequence-id-445462

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  42. kiCAP-AAV-MyoAAV2E

    Plasmid
    #224441
    Purpose
    Rep/Cap plasmid for the production of MyoAAV 2E, a muscle-tropic AAV capsid in mice.
    Depositor
    Pardis Sabeti
    Article
    Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.
    Insert
    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    Use
    AAV
    Tags
    Expression
    Mutation
    RGDQGYQ insert between amino acids 588 and 589 of…
    Promoter
    p41
    Available since
    Sept. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    224441-plasmid-map-sequence-id-446663

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  43. kiCAP-AAV-MyoAAV3D

    Plasmid
    #224445
    Purpose
    Rep/Cap plasmid for the production of MyoAAV 3D, a muscle-tropic AAV capsid in mice and macaques.
    Depositor
    Pardis Sabeti
    Article
    Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.
    Insert
    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    Use
    AAV
    Tags
    Expression
    Mutation
    RGDYREL insert between amino acids 588 and 589 of…
    Promoter
    p41
    Available since
    Sept. 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    224445-plasmid-map-sequence-id-446645

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  44. kiCAP-AAV-MyoAAV3E

    Plasmid
    #224446
    Purpose
    Rep/Cap plasmid for the production of MyoAAV 3E, a muscle-tropic AAV capsid in mice and macaques.
    Depositor
    Pardis Sabeti
    Article
    Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.
    Insert
    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    Use
    AAV
    Tags
    Expression
    Mutation
    RGDHGVL insert between amino acids 588 and 589 of…
    Promoter
    p41
    Available since
    Sept. 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    224446-plasmid-map-sequence-id-446644

    Enlarge

  45. kiCAP-AAV-MyoAAV4B

    Plasmid
    #224449
    Purpose
    Rep/Cap plasmid for the production of MyoAAV 4B, a muscle-tropic AAV capsid in mice and macaques.
    Depositor
    Pardis Sabeti
    Article
    Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.
    Insert
    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    Use
    AAV
    Tags
    Expression
    Mutation
    RGDYTSV insert between amino acids 588 and 589 of…
    Promoter
    p41
    Available since
    Sept. 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    224449-plasmid-map-sequence-id-446626

    Enlarge

  46. kiCAP-AAV-MyoAAV4D

    Plasmid
    #224451
    Purpose
    Rep/Cap plasmid for the production of MyoAAV 4D, a muscle-tropic AAV capsid in mice and macaques.
    Depositor
    Pardis Sabeti
    Article
    Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.
    Insert
    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    Use
    AAV
    Tags
    Expression
    Mutation
    RGDHGVL insert between amino acids 588 and 589 of…
    Promoter
    p41
    Available since
    Sept. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    224451-plasmid-map-sequence-id-446623

    Enlarge

  47. kiCAP-AAV-MyoAAV4C

    Plasmid
    #224450
    Purpose
    Rep/Cap plasmid for the production of MyoAAV 4C, a muscle-tropic AAV capsid in mice and macaques.
    Depositor
    Pardis Sabeti
    Article
    Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.
    Insert
    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    Use
    AAV
    Tags
    Expression
    Mutation
    RGDYTSM insert between amino acids 588 and 589 of…
    Promoter
    p41
    Available since
    Sept. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    224450-plasmid-map-sequence-id-446625

    Enlarge

  48. kiCAP-AAV-MyoAAV3C

    Plasmid
    #224444
    Purpose
    Rep/Cap plasmid for the production of MyoAAV 3C, a muscle-tropic AAV capsid in mice and macaques.
    Depositor
    Pardis Sabeti
    Article
    Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.
    Insert
    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    Use
    AAV
    Tags
    Expression
    Mutation
    RGDYSSV insert between amino acids 588 and 589 of…
    Promoter
    p41
    Available since
    Sept. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    224444-plasmid-map-sequence-id-446619

    Enlarge

  49. kiCAP-AAV-MyoAAV3B

    Plasmid
    #224443
    Purpose
    Rep/Cap plasmid for the production of MyoAAV 3B, a muscle-tropic AAV capsid in mice and macaques.
    Depositor
    Pardis Sabeti
    Article
    Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.
    Insert
    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    Use
    AAV
    Tags
    Expression
    Mutation
    RGDYSGL insert between amino acids 588 and 589 of…
    Promoter
    p41
    Available since
    Sept. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    224443-plasmid-map-sequence-id-446660

    Enlarge

  50. pBGE3.0

    Plasmid
    #165988
    Purpose
    Multi-host shuttle vector that can be transferred by conjugation and replicate in S. meliloti, E. coli, S. cerevisiae, and P. tricornutum. Contains S. meliloti pSymB origin and Nm/Kan resistance.
    Depositor
    Bogumil Karas
    Article
    Brumwell et al PLoS One. 2019 Jun 17;14(6):e0206781. do
    Inserts
    RK2/RP4 origin of transfer
    repA1B1C1
    nourseothricin N-acetyl transferase
    neomycin resistance
    Use
    Synthetic Biology; Bacterial and yeast cloning; c…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    165988-plasmid-map-sequence-id-378724

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Showing: 3151 - 3200 of 37659 results