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We narrowed to 18,759 results for: nls

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Showing: 2641 - 2680 of 18759 results

  2. pHeEf1a-mEGFP-NLS

    Plasmid
    #205012
    Purpose
    The vector contains 1kbp of the upstream and downstream regions of the ef1a gene from Hypsibius exemplaris, with the mEGFP gene with NLS sequence positioned in between.
    Depositor
    Kazuharu Arakawa
    Publication
    Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mEGFP-NLS flanked by 1kbp upstream and downstream of the ef1a gene from Hypsibius exemplaris
    Use
    Tardigrade expression
    Available since
    Aug. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    205012-plasmid-map-sequence-id-405256

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  3. PiggyBac-TDP-43deltaNLS-V5-APEX

    Plasmid
    #229081
    Purpose
    Doxycycline-inducible expression of NLS-mutated TDP-43 fused to V5-tag and APEX for proximity labeling, containing BFP in the backbone
    Depositor
    Eran Hornstein
    Insert
    TDP-43deltaNLS (TARDBP Human)
    Tags
    APEX, BFP, and V5
    Expression
    Mammalian
    Mutation
    K82A, R83A, K84A, K95A, K97A and R98A for mutatio…
    Promoter
    TRE3G
    Available since
    July 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    229081-plasmid-map-sequence-id-457517

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  4. plenti-As-Cas12a-2xNLS

    Plasmid
    #155047
    Purpose
    Lentiviral vector expressing human codon-optimized _C-terminal Myc-tagged _Acidaminococcus sp. BV3L6 (As)-Cas12a nuclease with N- and C-terminal NLS and Neomycin/G418/Geneticin resistance from EF-1alpha promoter
    Depositor
    Benjamin Blencowe, Jason Moffat
    Publication
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    AsCas12a
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS, Myc-tag and SV40 NLS
    Expression
    Mammalian
    Promoter
    EF-1a
    Available since
    Aug. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    155047-plasmid-map-sequence-id-308054

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  10. mApple-PCNA-19-NLS-4

    Plasmid
    #54937
    Purpose
    Localization: Nucleus, Excitation: 568, Emission: 592
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PCNA-19 NLS (PCNA Human)
    Tags
    mApple
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  11. LN-entry+NLS

    Plasmid
    #128011
    Purpose
    Vector containing the lambdaN-entry expression cassette with SV40 NLS and a separate expression cassette driving mCherry via the traffic jam enhancer
    Depositor
    Julius Brennecke
    Publication
    Batki et al Nat Struct Mol Biol. 2019 Aug;26(8):720-
    Insert
    LN-3xFlag-SV40NLS
    Use
    Lamdan entry vector with nls to generate tetherin…
    Tags
    LN-3xFlag-SV40NLS
    Available since
    Aug. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    128011-plasmid-map-sequence-id-251573

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  13. pET-28b-T7-hAsCas12a-enRVR(E174R/S542R/K548V/N552R)-NLS(nucleoplasmin)-6xHis (AAS1931)

    Plasmid
    #114075
    Purpose
    T7 promoter bacterial expression plasmid for human codon optimized AsCas12a-enRVR(E174R/S542R/K548V/N552R) with C-terminal NLS and His-tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized AsCas12a-enRVR (E174R/S542R/K548V/N552R) with C-terminal NLS and His-tag
    Tags
    NLS(nucleoplasmin) and 6xHis
    Expression
    Bacterial
    Mutation
    E174R, S542R, K548V and N552R
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    114075-plasmid-map-sequence-id-220517

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  15. pEJS581-pCSDest2-AcrIIC4Hpa-FLAG-NLS

    Plasmid
    #113436
    Purpose
    Expresses a FLAG/NLS tagged type II-C anti-CRISPR protein from H. parainfluenzae in mammalian cells
    Depositor
    Erik Sontheimer
    Publication
    Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    type II-C anti-CRISPR
    Use
    CRISPR
    Tags
    FLAG/NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Dec. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    113436-plasmid-map-sequence-id-225078

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  17. pP2A_NLS_sfGFP

    Plasmid
    #63709
    Purpose
    Expressing P2A_NLS_sfGFP with homologous arm of Nanog.
    Depositor
    Stanley Qi
    Publication
    Yu et al Cell Stem Cell. 2015 Feb 5;16(2):142-7.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Nanog homologous arm, p2A, NLS, sfGFP, Nanog homologous arm
    Use
    Just a donor dna with nanog homologous arm for kn…
    Tags
    sfGFP
    Available since
    April 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    63709-plasmid-map-sequence-id-107390

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  18. pICSL50059

    Plasmid
    #245614
    Purpose
    Level 0 Golden Gate part C-Terminal Tag with overhangs TTCG - GCTT
    Depositor
    Mark Youles
    Insert
    C-Terminal tag 2xBP NLS (2xGKRTADGSEFEPKKKRKV)
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245614-plasmid-map-sequence-id-495524

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  23. pCMV-T7-AcrIIA5-NLS(SV40) (KAC203)

    Plasmid
    #133802
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized AcrIIA5 with C-terminal NLS (SV40)
    Depositor
    Joseph Bondy-Denomy, Benjamin Kleinstiver
    Publication
    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized AcrIIA5
    Tags
    NLS(SV40)
    Expression
    Mammalian
    Mutation
    n/a
    Promoter
    CMV and T7
    Available since
    March 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    133802-plasmid-map-sequence-id-267958

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  24. pCMV-T7-AcrIIA4-NLS(SV40) (KAC200)

    Plasmid
    #133801
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized AcrIIA4 with C-terminal NLS (SV40)
    Depositor
    Joseph Bondy-Denomy, Benjamin Kleinstiver
    Publication
    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized AcrIIA4
    Tags
    NLS(SV40)
    Expression
    Mammalian
    Mutation
    n/a
    Promoter
    CMV and T7
    Available since
    March 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    133801-plasmid-map-sequence-id-267964

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  25. pCMV-T7-hAcrVA2.1-NLS(sv40) (BPK5059)

    Plasmid
    #115137
    Purpose
    CMV-T7 promoter expression plasmid for human codon optimized AcrVA2.1 anti-CRISPR protein with C-terminal NLS
    Depositor
    Joseph Bondy-Denomy, Keith Joung, Benjamin Kleinstiver
    Publication
    Marino et al Science. 2018 Sep 6. pii: science.aau517
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized AcrVA2.1 anti-CRISPR protein
    Tags
    NLS(SV40)
    Expression
    Mammalian
    Available since
    Sept. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    115137-plasmid-map-sequence-id-220492

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  29. pMT-myl7-NLS-BirA-2A-mCherry_Ras

    Plasmid
    #80061
    Purpose
    Myl7 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Publication
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
    Use
    Zebrafish biotagging
    Tags
    3x HA
    Expression
    Bacterial
    Promoter
    Myl7 promoter
    Available since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    80061-plasmid-map-sequence-id-152595

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  30. JAB3278

    Plasmid
    #190402
    Purpose
    Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-ERF2(AD)-19St_1XAmtRO(M2)::GFPint-NLS-ADHt)
    Depositor
    Jennifer Brophy, Jose Dinneny
    Publication
    Brophy et al Science. 2022 Aug 12;377(6607):747-751.
    Insert
    proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-ERF2(AD)-19St_1XAmtRO(M2)::GFPint-NLS-ADHt
    Expression
    Plant
    Available since
    May 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    190402-plasmid-map-sequence-id-393458

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  32. TDH3pro-rtTA-tetO7pro-T7pol-pRS413

    Plasmid
    #239293
    Purpose
    Expresses the reverse tetracycline activator (rtTA) and T7 polymerase; Plasmid #1 of the tet-on overexpression system
    Depositor
    Anita Hopper
    Publication
    Nostramo et al Mol Cell. 2025 Feb 20;85(4):726-741.e6.
    Inserts
    T7 polymerase with NLS and P278L
    reverse tetracycline transactivator (rtTA)
    Expression
    Yeast
    Mutation
    Contains M2-SE-G72P mutations, described in Addge…
    Promoter
    TDH3 and tetracycline-responsive element (TRE) an…
    Available since
    June 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    239293-plasmid-map-sequence-id-476828

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  34. pLJM1-CRS-Halo-NLS

    Plasmid
    #250859
    Purpose
    lentiviral vector for expressing HaloTag protein tagged with both a GDOWN1 cytoplasmic retention signal (CRS) and a SV40 NLS
    Depositor
    Britt Glaunsinger
    Insert
    HaloTag (POLR2M Human)
    Use
    Lentiviral
    Tags
    GDOWN1 (aa 250-368) and SV40 NLS
    Expression
    Mammalian
    Promoter
    CMV promoter
    Available since
    Feb. 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    250859-plasmid-map-sequence-id-498765

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Showing: 2641 - 2680 of 18759 results