We narrowed to 56,849 results for: plasmid
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Plasmid#124229PurposeTargets the human CLYBL safe harbor locus for integration of dox-inducible NGN2 for differentiation of iPSCs into cortical neurons. Contains a floxed BSD-NLS-mApple selection cassette.DepositorInsertNGN2 (NEUROG2 Human)
ExpressionMammalianAvailable SinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.GCaMP6f.WPRE.SV40
Plasmid#100836PurposeAAV expression of ultrasensitive protein calcium sensor from CAG promoterDepositorHas ServiceAAV1, AAV2, and AAV9InsertGCaMP6f
UseAAVExpressionMammalianMutationGCaMP3-T302L R303P A317E D380Y T381R S383T R392GPromoterCAGAvailable SinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato]
Plasmid#62723PurposeAAV-mediated expression of ChrimsonR-tdTomato under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal. TdTomato is a codon diversified version.DepositorHas ServiceAAV1 and AAV5InsertChrimsonR-tdTomato
UseAAVTagstdTomatoExpressionMammalianMutationChrimson K176R mutantPromoterhuman synapsin promoterAvailable SinceApril 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSynSRE-T-Luc
Plasmid#60444PurposeUses the hamster HMG-CoA synthase promoter sequence (-324/-225) and minimal HMG-CoA sythnase TATA box (-28/+39) to study SREBP-dependent transcriptional activation.DepositorInsertHMG-CoA synthase promoter (-324/-225)
UseLuciferaseTagsLuciferaseExpressionMammalianPromoterHMG-CoA synthase (-324/-225) & TATAA (-28/+39)Available SinceFeb. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
Cas9-T6N (pZZ12, TAT-4MycNLS-Cas9-2SV40NLS-sfGFP)
Plasmid#199604PurposeExpression of cell permeable spCas9 in E. Coli.DepositorInsertCas9-T6N
UseCRISPRExpressionBacterialAvailable SinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX330a dCas9-LSD1
Plasmid#92362PurposeModified CAG promoter-containing vector for ubiquitous expression of catalytically inactive Cas9 fused to LSD1. For targeted enhancer demethylation in chicken embryos.DepositorInsertdCas9-LSD1
UseCRISPRExpressionMammalianPromoterCAGAvailable SinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_NE1m
Plasmid#123308PurposeExpresses the genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1m in neuronsDepositorHas ServiceAAV Retrograde and AAV9InsertGPCR activation based NE sensor GRAB_NE1m
UseAAVMutationcontains a glycine-to-threonine mutation at posit…PromoterhSynAvailable SinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE
Plasmid#104495PurposeAAV-mediated expression of ultrasensitive protein calcium sensor under the CAG promoter, Cre mediated flip-excision switchDepositorHas ServiceAAV PHP.eB, AAV Retrograde, and AAV1InsertjGCaMP7s
UseAAV and Cre/LoxTagsT7 epitope, Xpress tag, 6xHisExpressionMammalianPromoterCAGAvailable SinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-mDlx-ChR2-mCherry-Fishell-3
Plasmid#83898PurposeChR2-mCherry fusion expression in forebrain GABA-ergic interneurons under the control of the mDlx enhancer elementDepositorHas ServiceAAV Retrograde, AAV1, and AAV9InsertChR2
UseAAVExpressionMammalianPromotermDlxAvailable SinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn-GRAB_DA2m
Plasmid#140553PurposeExpresses the 2nd generation medium-affinity DA sensor GRAB_DA2m in neuronsDepositorHas ServiceAAV9InsertThe 2nd generation DA sensor GRAB_DA2m (medium affinity)
UseAAVPromoterhSynAvailable SinceJuly 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-C1V1 (t/t)-TS-EYFP
Plasmid#35499PurposeAAV expression of CaMKII-driven chimeric opsin variant C1V1 (E122T/E162T) for fast and potent optical excitation at red-shifted wavelengths.DepositorInsertChR1-VChR1 Chimera
UseAAVTagsEYFPExpressionMammalianMutationE122T and E162TPromoterCaMKIIaAvailable SinceApril 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Sox2
Plasmid#101761PurposeSox2 promoter reporterDepositorAvailable SinceOct. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pY094
Plasmid#84743PurposeExpresses huAsCpf1-T2A-GFP and crRNA guideDepositorInsertshuAsCpf1
GFP
Tags3xHA, NLS, and T2AExpressionMammalianPromoterCMVAvailable SinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBA904
Plasmid#122238PurposeLentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs1 incorporated in the loop of the sgRNA constant region.DepositorInsertsgRNA with cs1 in stem loop 2 of the constant region
UseCRISPR and LentiviralExpressionMammalianAvailable SinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV.Syn.NES.jRCaMP1a.WPRE.SV40
Plasmid#100848PurposeAAV expression of jRCaMP1a, a red fluorescent calcium sensor protein, from the Synapsin promoterDepositorHas ServiceAAV1 and AAV9InsertjRCaMP1a
UseAAVExpressionMammalianPromoterSynapsinAvailable SinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-ihSyn1-tTA
Plasmid#99120PurposeAn AAV genome that expresses tTA from the hSyn1 promoter that contains a positive feedback loop for amplifed expression of the tTADepositorHas ServiceAAV1InserttTA
UseAAVExpressionMammalianMutation*(see below)PromoterihSyn1Available SinceAug. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-Chronos-GFP
Plasmid#59170PurposeAAV-mediated expression of Chronos-GFP under the Syn promoterDepositorHas ServiceAAV Retrograde, AAV1, and AAV5InsertChronos-GFP
UseAAVTagsGFPExpressionMammalianPromoterSynAvailable SinceAug. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-FLEX-jGCaMP8f-WPRE
Plasmid#162379PurposeAAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter, Cre-dependent expressionDepositorHas ServiceAAV Retrograde, AAV1, AAV5, and AAV9InsertjGCaMP8f
UseAAV and Cre/LoxTags6xHisExpressionMammalianMutationQ315LPromoterSynapsinAvailable SinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-mDlx-GCaMP6f-Fishell-2
Plasmid#83899PurposeGCaMP6f expression in forebrain GABA-ergic interneurons under the control of the mDlx enhancer elementDepositorHas ServiceAAV Retrograde, AAV1, and AAV9InsertGCaMP6f
UseAAVExpressionMammalianPromotermDlxAvailable SinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-mDlx-NLS-mRuby2
Plasmid#99130PurposeAn AAV genome that expresses nuclear localized mRuby2 from the mouse Dlx5/6 enhancerDepositorHas ServiceAAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9InsertNLS-mRuby2
UseAAVExpressionMammalianPromotermDLX5/6Available SinceAug. 3, 2017AvailabilityAcademic Institutions and Nonprofits only