We narrowed to 7,456 results for: Lif

1. pMIA4.721-Keppel

   Plasmid

   #214444

   Purpose

   BxbI landing-pad HDR donor for targeting Keppel-19 GSH. CAGG-driven Casp9-P2A-Bsd.

   Depositor

   Roger Foo

   Article

   Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755

   Insert

   2xFKBP12-Casp9
   

   Use

   Hdr donor

   Tags

   P2A-Bsd

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pENTR_L1-YFP-miniTurbo-L2

   Plasmid

   #127352

   Purpose

   gateway entry vector for making N-terminal YFP-miniTurbo fusions with nuclear and non-nuclear proteins

   Depositor

   Dominique Bergmann

   Article

   Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

   Insert

   miniTurbo (BirA mutant)
   

   Use

   Gateway entry vector

   Tags

   GS linker, V5, and YFP

   Expression

   Mutation

   aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

   Promoter

   no promoter

   Available since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pENTR_L1-YFP-miniTurbo-NES-L2

   Plasmid

   #127351

   Purpose

   gateway entry vector for making N-terminal YFP-miniTurbo fusions with non-nuclear proteins

   Depositor

   Dominique Bergmann

   Article

   Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

   Insert

   miniTurbo (BirA mutant)
   

   Use

   Gateway entry vector

   Tags

   GS linker, NES, V5, and YFP

   Expression

   Mutation

   aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

   Promoter

   no promoter

   Available since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. p130Cas Y15F

   Plasmid

   #186154

   Purpose

   For expressing recombinant p130Cas in insect cells.

   Depositor

   Michael Rosen

   Article

   Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

   Insert

   BCAR1 (Bcar1 Rat)
   

   Use

   Tags

   His6 (Tev)

   Expression

   Insect

   Mutation

   15Y residues mutated to Alanine

   Promoter

   polyhedrin

   Available since

   June 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. Sema6c-AP-His

   Plasmid

   #72041

   Purpose

   Expresses the extracellular region of the Sema6C protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Sema6c (Sema6c Mouse)
   

   Use

   Tags

   AP-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-ROR2-V5/HIS

   Plasmid

   #205608

   Purpose

   Expression of human ROR2 receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   ROR2 receptor tyrosine kinase (ROR2 Human)
   

   Use

   Tags

   V5-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-Sst12-GFP

   Plasmid

   #172311

   Purpose

   SST interneuron-restricted gene regulatory element GRE12, to drive GFP expression in SST+ interneurons

   Depositor

   Michael Greenberg

   Article

   Hrvatin et al Elife. 2019 Sep 23;8. pii: 48089. doi: 1

   Insert

   Sst12
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   pB-Globin

   Available since

   July 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDSP16 (5’Δ Cbr-unc-119(+) + loxN + MCS + 5’Δ mNeonGreen)

   Plasmid

   #193854

   Purpose

   Promoterless insertion vector for split Cbr-unc-19(+)/mNeonGreen landing pad

   Depositor

   Patrick Phillips

   Article

   Stevenson et al Elife. 2023 Jul 4;12:RP84831. doi: 10.75

   Insert

   5' Cbr-unc-119(+) + 5' mNeonGreen
   

   Use

   CRISPR and Cre/Lox

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Cbr-unc-119p

   Available since

   Sept. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. mAMCase_CatD_6xHis_pTwistCMV

   Plasmid

   #200228

   Purpose

   Expresses mouse Acidic Mammalian Chitinase catalytic domain with C-terminal 6xHis tag in pTwist CMV vector

   Depositor

   James S Fraser

   Article

   Diaz et al Elife. 2024 Jun 17;12:RP89918. doi: 10.7

   Insert

   Acidic Mammalian Chitinase (Chia1 Mouse)
   

   Use

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   S354F

   Promoter

   CMV

   Available since

   Sept. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. Sema6d.4-AP-His

    Plasmid

    #72044

    Purpose

    Expresses the extracellular region of the Sema6D, isoform 4 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sema6d.4 (Sema6d Mouse)
    

    Use

    Tags

    AP-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. MSCV-mir-17-92

    Plasmid

    #64100

    Purpose

    expresses mir-17-92

    Depositor

    Lin He

    Article

    Olive et al Elife. 2013 Oct 15;2:e00822. doi: 10.755

    Insert

    miR-17-92 (MIR17HG Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV

    Available since

    June 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFastBac_His-ZZ-TEV-Lis1

    Plasmid

    #132539

    Purpose

    Plasmid for insect cell expression of human full-length LIS1 with an N-terminal His-ZZ-LTLT tag

    Depositor

    Andrew Carter

    Article

    Baumbach et al Elife. 2017 Apr 13;6. pii: e21768. doi:

    Insert

    Lis1 (PAFAH1B1 Human)
    

    Use

    Tags

    8x His, Linker-TEV-linker_TEV, and ZZ

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBABEpuro mTurquoise2 LC3B

    Plasmid

    #78518

    Purpose

    Fluorescent reporter for LC3B visualization

    Depositor

    David Chan

    Article

    Rojansky et al Elife. 2016 Nov 17;5. pii: e17896. doi:

    Insert

    LC3B (MAP1LC3B Human)
    

    Use

    Retroviral

    Tags

    mTurquoise2

    Expression

    Mammalian

    Mutation

    Promoter

    retroviral

    Available since

    Jan. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMIA4.721-Olônne

    Plasmid

    #214443

    Purpose

    BxbI landing-pad HDR donor for targeting Olônne-18 GSH. CAGG-driven Casp9-P2A-Bsd.

    Depositor

    Roger Foo

    Article

    Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755

    Insert

    2xFKBP12-Casp9
    

    Use

    Hdr donor

    Tags

    P2A-Bsd

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1-ROS1-V5/HIS

    Plasmid

    #205609

    Purpose

    Expression of human ROS1 receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    ROS1 receptor tyrosine kinase (ROS1 Human)
    

    Use

    Tags

    V5-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti-CMV-DNAJC5

    Plasmid

    #205727

    Purpose

    Mammalian expression of DNAJC5

    Depositor

    Randy Schekman

    Article

    Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

    Insert

    DNAJC5 (DNAJC5 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLNCX2-mEGFP-CHMP2A

    Plasmid

    #115328

    Purpose

    expresses mEGFP-CHMP2A in mammalian cells

    Depositor

    Sanford Simon

    Article

    Johnson et al Elife. 2018 Jul 4;7. pii: 36221. doi: 10

    Insert

    CHMP2A (CHMP2A Human)
    

    Use

    Retroviral

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEMHE-mEGFP-Map4

    Plasmid

    #105571

    Purpose

    T7 promotor drives in vitro transcription of mEGFP-tagged Histon MAP4 mRNA

    Depositor

    Melina Schuh

    Article

    Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

    Insert

    MAP4 (Map4 Mouse)
    

    Use

    In vitro transcription

    Tags

    mEGFP

    Expression

    Mutation

    Promoter

    T7

    Available since

    March 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-Sst22-GFP

    Plasmid

    #172312

    Purpose

    SST interneuron-restricted gene regulatory element GRE22, to drive GFP expression in SST+ interneurons

    Depositor

    Michael Greenberg

    Article

    Hrvatin et al Elife. 2019 Sep 23;8. pii: 48089. doi: 1

    Insert

    Sst22
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    pB-Globin

    Available since

    July 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. VEGFR2-YFP

    Plasmid

    #108853

    Purpose

    Encodes for human VEGFR2 fluorescently labeled with eYFP on the C-Terminus via a 3 amino acid (GGS) flexible linker

    Depositor

    Kalina Hristova

    Article

    Sarabipour et al Elife. 2016 Apr 7;5:e13876. doi: 10.7554

    Insert

    KDR (KDR Human)
    

    Use

    Tags

    Labeled with eYFP on the C-Terminus via a 3 amino…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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21. Ntn1-AP-His

    Plasmid

    #71978

    Purpose

    Expresses the entire Netrin 1 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Ntn1 (Ntn1 Mouse)
    

    Use

    Tags

    AP-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAAV-DIO-Ef1a-TVA-2A-oG

    Plasmid

    #172359

    Purpose

    Targeting envA-pseodotyped RVdG-CVS-N2c vectors for specific retrograde labeling specifically from cre-expressing neurons

    Depositor

    Peter Jonas, Yoav Ben-Simon

    Article

    Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

    Insert

    TVA + B19-oG
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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23. pCA528 AMSH 1-178

    Plasmid

    #160050

    Purpose

    Expresses MIT domain (residues 1-178) of AMSH as a HIS-SUMO fusion protein. Internal ID: WISP18-22.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    AMSH residues 1-178 (STAMBP Human)
    

    Use

    Tags

    HIS-SUMO

    Expression

    Bacterial

    Mutation

    Residues 1-178

    Promoter

    T7

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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24. pENTR_L1-YFP-Turbo-NES-L2

    Plasmid

    #127349

    Purpose

    gateway entry vector for making N-terminal YFP-TurboID fusions with non-nuclear proteins

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    TurboID (BirA mutant)
    

    Use

    Gateway entry vector

    Tags

    GS linker, NES, V5, and YFP

    Expression

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Promoter

    no promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. AAVS1-SA-neo-CAGGS-PE2-2A-GFP

    Plasmid

    #180014

    Purpose

    Targeting vector for knocking in constitutively expressed PE2-2A-GFP into the human AAVS1 locus

    Depositor

    Dirk Hockemeyer

    Article

    Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

    Insert

    PE2-2A-GFP
    

    Use

    CRISPR; Human genome engineering

    Tags

    GFP

    Expression

    Mutation

    Promoter

    CAG

    Available since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pTol1-UAS:GtACR1-tdTomato

    Plasmid

    #124235

    Purpose

    Zebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin GtACR1 fused to the red fluorescent protein tdTomato

    Depositor

    Isaac Bianco

    Article

    Antinucci et al Elife. 2020 Mar 27;9. pii: 54937. doi: 1

    Insert

    GtACR1-tdTomato
    

    Use

    Zebrafish expression

    Tags

    tdTomato

    Expression

    Mutation

    Promoter

    14xUAS-E1b

    Available since

    Jan. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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27. pEf1a OsTIR ires NEO pA T2BH

    Plasmid

    #127910

    Purpose

    Transposon vector containing an OsTIR IRES Neo cassette driven by the Ef1a promoter

    Depositor

    Mark Groudine

    Article

    Strom et al Elife. 2021 Jun 9;10. pii: 63972. doi: 1

    Insert

    OsTIR
    

    Use

    Transposon

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. mCerulean3-BclXL-pEGFP-C1

    Plasmid

    #177408

    Purpose

    To express mCerulean3 fused to the N-terminus of the Bcl-2 family protein, Bcl-XL.

    Depositor

    David Andrews

    Article

    Liu et al Elife. 2019 Mar 12;8. pii: 37689. doi: 1

    Insert

    Bcl-XL
    

    Use

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pFE-Selo-FI-prk

    Plasmid

    #162698

    Purpose

    Expresses S. elongatus Form IB rubisco and S. elongatus Prk

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Insert

    S. elongatus Form IB rubisco and S. elongatus Prk
    

    Use

    Tags

    N terminal 6x His tag on PRK

    Expression

    Bacterial

    Mutation

    Promoter

    PLtet0-1 promoter

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pHAGE2 GFP-Atl1

    Plasmid

    #86679

    Purpose

    Lentivirus to stably express fluorescent protein in mammalian cells

    Depositor

    Tom Rapoport

    Article

    Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

    Insert

    ATL1 (ATL1 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge