162,676 results

1. pAAV2/rh10

   Plasmid

   #112866

   Purpose

   AAV packaging plasmid expressing Rep/Cap genes

   Depositor

   James M. Wilson

   Insert

   Rep2/Cap-rh10
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Truncated P5

   Available since

   Sept. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. lentiCRISPRv2 puro

   Plasmid

   #98290

   Purpose

   Variant of lentiCRISPRv2 that confers puromycin resistance

   Depositor

   Brett Stringer

   Article

   Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF-1a

   Available since

   July 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. mCherry-Mito-7

   Plasmid

   #55102

   Purpose

   Localization: Mitochondria, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Article

   Olenych et al Curr Protoc Cell Biol. 2007 Sep;Chapter

   Insert

   Mito (COX8A Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV[Expression]-mCherry:T2A:Hygro-EF1A>Luc2

   Plasmid

   #174665

   Purpose

   Expresses humanized firefly luciferase and mCherry and Hygromycin resistance linked by T2A, each controlled by a strong promoter

   Depositor

   Eli Bar

   Inserts

   LUC2
   mCherry:T2A:Hygro
   

   Use

   Lentiviral and Luciferase

   Tags

   mCherry and hygromycin dual reporter gene linked …

   Expression

   Mammalian

   Mutation

   Promoter

   Human cytomegalovirus immediate early enhancer/pr…

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. VPS13A^mCherry

   Plasmid

   #118758

   Purpose

   VPS13A internally tagged with mCherry at residue 1372.

   Depositor

   Pietro De Camilli

   Article

   Kumar et al J Cell Biol. 2018 Oct 1;217(10):3625-363

   Insert

   VPS13A (VPS13A Human)
   

   Use

   Tags

   mCherry inserted at position 1372

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. XLone-GFP

   Plasmid

   #96930

   Purpose

   Tunable and temporal expression control of GFP

   Depositor

   Xiaojun Lian

   Article

   Randolph et al Sci Rep. 2017 May 8;7(1):1549. doi: 10.1

   Insert

   Green Fluorescent Protein
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3GS

   Available since

   Aug. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. LgBiT-LentiB3 Transfer Vector

   Plasmid

   #236912

   Purpose

   Lentiviral packaging plasmid for generation of LgBiT lentiviral particles to transduce HiBiT cell lines

   Depositor

   Promega Corporation

   Has Service

   DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   LgBiT

   Expression

   Mutation

   None

   Promoter

   Available since

   July 14, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pcDNA3-AKAP79-AKAR4

   Plasmid

   #181957

   Purpose

   Genetically encoded FRET-based PKA activity reporter fused to AKAP79.

   Depositor

   Jin Zhang

   Article

   Tenner et al Elife. 2020 Nov 17;9. pii: 55013. doi: 1

   Insert

   AKAP79-AKAR4 (AKAP5 Human)
   

   Use

   Tags

   AKAP79, Cerulean, HIS tag, and cpVenus(E172)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1 Dendra2

   Plasmid

   #103967

   Purpose

   Mammalian expression of fluorescent protein Dendra2 used for transfection efficiency control

   Depositor

   Qing Deng

   Article

   Hsu et al Dis Model Mech. 2017 Sep 27. pii: dmm.03

   Insert

   Dendra2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. TurboID-CRaf

    Plasmid

    #226168

    Purpose

    Lentiviral plasmid used to express a TurboID fusion of the protein kinase CRaf.

    Depositor

    Dustin Maly

    Article

    Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

    Insert

    Myc-TurboID-CRaf (RAF1 Human)
    

    Use

    Lentiviral

    Tags

    EGFP-IRES-BlastR and Myc-TurboID

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. PEA1-Puro

    Plasmid

    #171991

    Purpose

    Delivers all prime editing nickase components in a single, puromycin selectable plasmid

    Depositor

    Paul Thomas

    Article

    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

    Insert

    CbH-Cas9(H840A)-RT-T2A-Puro, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV for Cas9, U6 for gRNAs

    Available since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEMG-GRIS

    Plasmid

    #225134

    Purpose

    For genome editing in P. putida KT2440, rhamnose is used for the induction of I-sceI gene. Gm.

    Depositor

    Lars Blank

    Article

    Meng et al Microb Biotechnol. 2024 Jul;17(7):e14531

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 10, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pMK419 (GAL-OsTIR1(F74G))

    Plasmid

    #140656

    Purpose

    GAL-OsTIR1(F74G)

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    GAL-OsTIR1(F74G)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRK5-EGFP-Tau P301L

    Plasmid

    #46908

    Purpose

    expresses EGFP tagged Tau P301L in mammalian cells

    Depositor

    Karen Ashe

    Article

    Hoover et al Neuron. 2010 Dec 22;68(6):1067-81. doi:

    Insert

    Tau P301L (MAPT Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    P301L

    Promoter

    CMV

    Available since

    July 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA

    Plasmid

    #61591

    Purpose

    A single vector AAV-Cas9 system containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA.

    Depositor

    Feng Zhang

    Article

    Ran et al Nature. 2015 Apr 1. doi: 10.1038/nature1

    Inserts

    hSaCas9
    Chimeric guide for SaCas9
    

    Use

    AAV and CRISPR

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-enCas12a-ABE8e-V106W

    Plasmid

    #193653

    Purpose

    Expression of base editor enCas12a-ABE8e-V106W in mammalian cells for base editing, along with non-fused EGFP fluorescence

    Depositor

    Liangxue Lai

    Article

    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

    Insert

    dLb-enCas12a-TadA*8e-V106W, EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    dLbCas12a-D156R/G532R/K538R, TadA*8e-V106W

    Promoter

    CMV

    Available since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAc5.1B-EGFP

    Plasmid

    #21181

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

    Insert

    enhanced green flourescent protein
    

    Use

    Drosophila expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCE-mp53DD

    Plasmid

    #41856

    Purpose

    Non-integrating (episomal) expression of mouse p53DD - p53 carboxy-terminal dominant-negative fragment

    Depositor

    Shinya Yamanaka

    Article

    Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

    Insert

    Trp53 (Trp53 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Δ40-903

    Promoter

    CAG

    Available since

    July 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCS2+HyPer7-NES

    Plasmid

    #136467

    Purpose

    Mammalian expression of cytoplasm targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

    Depositor

    Vsevolod Belousov

    Article

    Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

    Insert

    HyPer7
    

    Use

    Tags

    Nuclear export signal from the HIV Rev protein

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, SP6

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. PPRE X3-TK-luc

    Plasmid

    #1015

    Purpose

    PPAR reporter. 3xDR1 sites upstream of a luciferase reporter.

    Depositor

    Bruce Spiegelman

    Article

    Kim et al Proc Natl Acad Sci U S A 1998 Apr 14;95(

    Insert

    PPRE
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 3, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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