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pBabe N-Ras 61K

Plasmid

#12543

Depositor

Channing Der

Publication

Khosravi-Far et al Mol Cell Biol. 1996 Jul . 16(7):3923-33.

Insert

NRas 61K (NRAS Human)

Use

Retroviral

Expression

Mammalian

Mutation

61K constitutively activated.

Available since

Sept. 8, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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Raptor_2 shRNA

Plasmid

#1858

Depositor

David Sabatini

Publication

Sarbassov et al Science 2005 Feb 18;307(5712):1098-101.

Insert

Raptor (RPTOR Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available since

April 5, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

20 Articles

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pLVX-EF1alpha-SARS-CoV-2-M-2xStrep-IRES-Puro

Plasmid

#141386

Purpose

Lentiviral expression of SARS-CoV-2 membrane (M) protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Publication

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-M (M SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available since

April 8, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

16 Articles

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pXPR_050

Plasmid

#96925

Purpose

lentiviral expression of gRNA scaffold

Depositor

John Doench, David Root

Publication

Sanson et al Nat Commun. 2018 Dec 21;9(1):5416. doi:

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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ER-GCaMP6-150

Plasmid

#86918

Purpose

Fluorescent reporter for ER calcium signaling

Depositor

Timothy Ryan

Publication

de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

Insert

ER-GCaMP6-150

Use

Lentiviral

Expression

Mammalian

Mutation

K78H, T302L, R303P, A317E, D324G, D360G, D380Y, T…

Promoter

CAMKII

Available since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

28 Articles

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pMK286 (mAID-NeoR)

Plasmid

#72824

Purpose

C-terminal tagging with mAID using CRISPR/Cas9

Depositor

Masato Kanemaki

Publication

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mAID-Neo

Use

CRISPR

Tags

mAID

Expression

Mammalian

Available since

March 24, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pcDNA3-mCardinal

Plasmid

#51311

Purpose

Expresses far-red fluorescent protein mCardinal in mammalian cells

Depositor

Michael Lin

Publication

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

mCardinal

Expression

Mammalian

Promoter

CMV

Available since

April 8, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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FH-Tet2-pEF

Plasmid

#41710

Purpose

This plasmid expresses mouse Tet2 with N-terminal FLAG and HA tags in a mammalian system

Depositor

Anjana Rao

Publication

Ko et al Nature. 2010 Dec 9;468(7325):839-43. doi

Insert

tet methylcytosine dioxygenase 2 (Tet2 Mouse)

Tags

FLAG and HA

Expression

Mammalian

Promoter

EF1a

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pDisplay-mSA-EGFP-TM

Plasmid

#39863

Depositor

Sheldon Park

Publication

Lim et al Biotechnol Bioeng. 2013 Jan;110(1):57-67

Insert

monomeric streptavidin, EGFP, TM helix

Tags

EGFP, FLAG, and HA

Expression

Mammalian

Promoter

CMV

Available since

March 15, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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phage-cmv-cfp-24xms2

Plasmid

#40651

Depositor

Robert Singer

Publication

Wu et al Biophys J. 2012 Jun 20;102(12):2936-44.

Insert

CFP-24xMBS

Use

Lentiviral

Tags

CFP

Expression

Mammalian

Promoter

CMV

Available since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pVSV eGFP dG

Plasmid

#31842

Depositor

Connie Cepko

Publication

Beier et al Proc Natl Acad Sci U S A. 2011 Sep 13;10

Insert

VSV genome

Expression

Bacterial

Available since

Oct. 1, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

30 Articles

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pENTR1A-Huwe1

Plasmid

#37431

Depositor

Jean Cook

Insert

Huwe1 (HUWE1 Human)

Use

Gateway Cloning

Available since

Sept. 25, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pT3-myr-AKT-HA

Plasmid

#31789

Depositor

Xin Chen

Publication

Calvisi et al Gastroenterology. 2011 Mar;140(3):1071-8

Insert

Akt1 (Akt1 Mouse)

Tags

HA and Myr

Expression

Mammalian

Available since

Nov. 16, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

45 Articles

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pET His6 Sumo TEV LIC cloning vector (2S-T)

Plasmid

#29711

Depositor

Scott Gradia

Type

Empty backbone

Tags

6xHis, SUMO, and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

28 Articles

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hTLR4

Plasmid

#13086

Depositor

Ruslan Medzhitov

Insert

TLR4 (TLR4 Human)

Expression

Mammalian

Available since

Oct. 3, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pLX313-Renilla luciferase

Plasmid

#118016

Purpose

Constitutive expression of Renilla luciferase

Depositor

William Hahn, David Root

Publication

Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

Insert

Renilla luciferase

Use

Lentiviral

Expression

Mammalian

Promoter

EF1alpha

Available since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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pCMV_AncBE4max

Plasmid

#112094

Purpose

C:G-to-T:A base editing

Depositor

David Liu

Publication

Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

Insert

AncBE4max

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

48 Articles

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miniTurbo-His6_pET21a

Plasmid

#107178

Purpose

expresses His6-tagged miniTurbo in bacteria

Depositor

Alice Ting

Publication

Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

Insert

miniTurbo (BirA mutant)

Expression

Bacterial

Mutation

aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

Available since

May 4, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMK293 (mAID-mCherry2-Hygro)

Plasmid

#72831

Purpose

C-terminal tagging with mAID-mCherry2 using CRISPR/Cas9

Depositor

Masato Kanemaki

Publication

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mAID-mCherry2-Hygro

Use

CRISPR

Tags

mAID-mCherry2

Expression

Mammalian

Available since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pLPCX mito Grx1-roGFP2

Plasmid

#64977

Purpose

Mammalian expression of mitochondrial Grx1-roGFP2 (retroviral vector)

Depositor

Tobias Dick

Publication

Gutscher et al Nat Methods. 2008 Jun;5(6):553-9. doi: 1

Insert

Glutaredoxin-1 (GLRX Human)

Use

Retroviral

Tags

roGFP2

Expression

Mammalian

Mutation

mitochondrial targeting sequence

Available since

June 17, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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