Addgene

We narrowed to 13,524 results for: TIM

Showing: 2801 - 2840 of 13524 results

Results per page:

Sort results by:

KA1870_pGL4-UBCmin-luc2/Esrrb-B'

Plasmid

#124196

Purpose

Luciferase reporter driven by the Esrrb enhancer

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Insert

Esrrb enhancer

Use

Luciferase

Tags

Expression

Mutation

Promoter

UBCmin

Available since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMOS030E: Entry vector for: ratiometric pHluorin (endoplasmic reticulum)

Plasmid

#163071

Purpose

Entry vector for: ratiometric pHluorin (endoplasmic reticulum)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

ratiometric pHluorin

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
KA1869_pGL4-UBCmin-luc2/Fgf4-A'

Plasmid

#124195

Purpose

Luciferase reporter driven by the Fgf4 enhancer

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Insert

Fgf4 enhancer

Use

Luciferase

Tags

Expression

Mutation

Promoter

UBCmin

Available since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMOS019E: Entry vector for: ClopHensorN chloride sensor (cytosolic)

Plasmid

#163066

Purpose

Entry vector for: ClopHensorN chloride sensor (cytosolic)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

ClopHensorN

Use

Gateway entry vector

Tags

tdTomato

Expression

Mutation

Promoter

Available since

April 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET3a RegIIIg

Plasmid

#64936

Purpose

bacterial expression of processed mouse RegIIIgamma

Depositor

Lora Hooper

Article

Mukherjee et al J Biol Chem. 2009 Feb 20;284(8):4881-8.

Insert

RegIIIg (Reg3g Mouse)

Use

Tags

Expression

Bacterial

Mutation

processed form

Promoter

T7

Available since

June 25, 2015

Availability

Academic Institutions and Nonprofits only
EKAR2G_design1_mTFP_227_Venus_173

Plasmid

#39835

Depositor

Olivier Pertz

Article

Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

Insert

Erk activity reporter

Use

Tags

His-Myc

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

CMV

Available since

July 5, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA5/FRT/TO_Lyn11-STf

Plasmid

#182008

Purpose

CMV overexpression of SNAP-tag fast at the inner leaflet of the plasma membrane of mammalian cell lines

Depositor

Kai Johnsson

Article

Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

Insert

Lyn11-STf

Use

Tags

Expression

Mammalian

Mutation

Fast mutation on SNAP-tag E30R

Promoter

CMV

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
BII-CPspaH2B

Plasmid

#133385

Purpose

Piggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR)

Depositor

Blair Madison

Article

Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
ptagBFP-LacI-NLS-VP16

Plasmid

#103837

Purpose

Transcription activator (transactivation domain of viral VP16 protein) that is constitutively recruited lacO arrays

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

tagBFP-LacI-NLS-VP16

Use

Tags

Expression

Mammalian

Mutation

Promoter

SV2

Available since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pME-QUAS5x:GFPNLS-SV40pA

Plasmid

#155115

Purpose

Middle entry vector containing QUAS5x element upstream of GFP-NLS.

Depositor

Bret Pearson

Article

Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

Insert

QUAS5x:GFPNLS

Use

Gateway middle entry vector

Tags

Expression

Mutation

Promoter

QUAS5x-E1b

Available since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-BsCas12a-2AeGFP

Plasmid

#123633

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

Insert

BsCas12a

Use

Tags

3xHA tag

Expression

Mammalian

Mutation

Promoter

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTrichoGate-5

Plasmid

#160705

Purpose

Trichoderma optimised Synthetic eGFP bordered by BsaI restriction sites

Depositor

Artemio Mendoza

Article

Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

Insert

Trichoderma optimised Synthetic eGFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pYES2.1-cerMET5

Plasmid

#107437

Purpose

Overexpression of MET fusion protein in S. cerevisiae

Depositor

Clare O'Connor

Article

Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

Insert

MET5 (MET5 Budding Yeast)

Use

Tags

V5-His

Expression

Yeast

Mutation

V81I, K341E, P597L, Y605C, E741G, D932G, N1217S, …

Promoter

GAL1

Available since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCytERM-EBFP2

Plasmid

#184939

Purpose

ER Visualization (OSER assay) Expresses EBFP2 in fusion with N-terminal part of p450

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

CytERM-EBFP2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLN397 (FuGW-S(E2F1)p-Sponge7)

Plasmid

#105181

Purpose

Module 2 - synthetic promoter E2F1 drives sponge 7 ,"optimized sponge" expression. (see PMID: 29056342 for detailed information)

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

S(E2F1)p-Sponge7

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pNB330

Plasmid

#60147

Purpose

Disintegrator plasmid encoding MET17 promoter driven NanoLuc (Promega) with nuclear localization signal (NLS)

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

NLuc

Use

Tags

Nuclear localization signal (NLS)

Expression

Yeast

Mutation

Promoter

MET17pr

Available since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pTrichoGate-11

Plasmid

#160711

Purpose

For C-terminal tags with Trichoderma optimised Synthetic eGFP bordered by BsaI restriction sites

Depositor

Artemio Mendoza

Article

Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

Insert

Trichoderma optimised Synthetic eGFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pETM6-Vv4CL-PhCHS-CmCHI

Plasmid

#73401

Purpose

Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

Depositor

Mattheos Koffas

Article

Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

Inserts

Vv4CL
PhCHS
CmCHI

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Nabi2.244

Plasmid

#73756

Purpose

Nabi2.244 is a FRET (Förster Resonance Energy Transfer)-based protein voltage sensor. This probe contains Clover and mRuby2 inserted at different locations in the Ciona voltage sensitive domain.

Depositor

Bradley Baker

Article

Sung et al PLoS One. 2015 Nov 20;10(11):e0141585. d

Insert

Nabi2.244

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 7, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pETM6-Pc4CL-PhCHS-MsCHI

Plasmid

#73396

Purpose

Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

Depositor

Mattheos Koffas

Article

Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

Inserts

Pc4CL
PhCHS
MsCHI

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 14, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
RV-Cag-Dio-mEYFP

Plasmid

#87666

Purpose

retroviral vector encoding cre dependent expression of membrane EYFP

Depositor

Oscar Marin, Lynette Lim

Article

Lim et al Nat Neurosci. 2018 Jun 18. pii: 10.1038/

Insert

Mem-EYFP

Use

Retroviral

Tags

palmitylation

Expression

Mutation

Promoter

CAG

Available since

May 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pACBB-TetT-LVA

Plasmid

#32555

Depositor

Claudia Schmidt-dannert

Article

Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

Insert

TetR

Use

Synthetic Biology

Tags

LVA tag

Expression

Bacterial

Mutation

Promoter

Consitutive lac

Available since

Nov. 8, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
KA1874_pGL4-UBCmin-luc2/Sox2_SRR1

Plasmid

#124197

Purpose

Luciferase reporter driven by the Sox2 enhancer

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Insert

Sox2 enhancer

Use

Luciferase

Tags

Expression

Mutation

Promoter

UBCmin

Available since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
KA2627_pGL4-UBCmin-luc2/Jam2-A'

Plasmid

#124200

Purpose

Luciferase reporter driven by the Jam2 enhancer

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Insert

Jam2 enhancer

Use

Luciferase

Tags

Expression

Mutation

Promoter

UBCmin

Available since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
KA1867_pGL4-UBCmin-luc2/Pou5f1_CR2

Plasmid

#124193

Purpose

Luciferase reporter driven by the Pou5f1 enhancer

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Insert

Pou5f1 enhancer

Use

Luciferase

Tags

Expression

Mutation

Promoter

UBCmin

Available since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
KA1866_pGL4-UBCmin-luc2/Pou5f1_CR3

Plasmid

#124192

Purpose

Luciferase reporter driven by the Pou5f1 enhancer

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Insert

Pou5f1 enhancer

Use

Luciferase

Tags

Expression

Mutation

Promoter

UBCmin

Available since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
KA1865_pGL4-UBCmin-luc2/Pou5f1_CR4

Plasmid

#124191

Purpose

Luciferase reporter driven by the Pou5f1 enhancer

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Insert

Pou5f1 enhancer

Use

Luciferase

Tags

Expression

Mutation

Promoter

UBCmin

Available since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
KA1403_pGL4-UBCmin-luc2/Nanog-B'

Plasmid

#124190

Purpose

Luciferase reporter driven by the Nanog enhancer

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Insert

Nanog enhancer

Use

Luciferase

Tags

Expression

Mutation

Promoter

UBCmin

Available since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
KA1402_pGL4-UBCmin-luc2/Nanog-A'

Plasmid

#124189

Purpose

Luciferase reporter driven by the Nanog enhancer

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Insert

Nanog enhancer

Use

Luciferase

Tags

Expression

Mutation

Promoter

UBCmin

Available since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
EKAR2G_design1_mTFP_wt_Venus_157

Plasmid

#39814

Depositor

Olivier Pertz

Article

Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

Insert

Erk activity reporter

Use

Tags

His-Myc

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

CMV

Available since

July 5, 2013

Availability

Academic Institutions and Nonprofits only
pMOS031E: Entry vector for: superecliptic pHluorin (endoplasmic reticulum)

Plasmid

#163068

Purpose

Entry vector for: superecliptic pHluorin (endoplasmic reticulum)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

superecliptic pHluorin

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pYFP-lacY-.008

Plasmid

#140140

Purpose

Evaluating the impact of feedback and low sensor concentration on information transmission of a genetic sensor.

Depositor

Drew Tack

Article

Sarkar et al Commun Biol. 2020 Apr 30;3(1):203. doi:

Insert

lacY (lacY )

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 29, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Crimson/P2A-NLS/HA-HumBeta (pSLIK2)

Plasmid

#113857

Purpose

For Doxycycline-inducible expression of Humanized Beta synaptase gene, with red fluorescent protein gene E2-Crimson-P2A-Humanized Beta synaptase gene

Depositor

Richard S Myers

Article

Valledor et al PLoS One. 2018 Aug 15;13(8):e0200955. do

Insert

Humanized Beta

Use

Lentiviral

Tags

P2A/Crimson

Expression

Mutation

"humanized" Beta gene

Promoter

Available since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pJL6

Plasmid

#184134

Purpose

For labelling (RFP) the apical region of the intestine. Translational fusion construct. coel::RFP_Pvha-6::vha-6::dsred.

Depositor

Alejandro Aballay

Article

Lalsiamthara et al Commun Biol. 2022 May 5;5(1):422. doi: 1

Insert

CELE_VW02B12L.1 (vha-6 Nematode)

Use

Tags

RFP

Expression

Worm

Mutation

Promoter

Pvha-6 (894bp)

Available since

June 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-PrCas12a-2AeGFP

Plasmid

#123636

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

Insert

PrCas12a

Use

Tags

3xHA tag

Expression

Mammalian

Mutation

Promoter

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pKT-SCFP3A-HIS

Plasmid

#64686

Purpose

pKT vector encoding yeast-codon optimized and fast-folding cyan fluorescent protein SCFP3A.

Depositor

Erin O'Shea

Article

Hansen et al Mol Syst Biol. 2013 Nov 5;9:704. doi: 10

Insert

SCFP3A

Use

Tags

Expression

Yeast

Mutation

Nucleotide sequence has been yeast-codon optimize…

Promoter

Available since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS-PtH

Plasmid

#133391

Purpose

Piggybac vector for SRIRACCHA-mediated mutation enrichment (donor repair template for HDR)

Depositor

Blair Madison

Article

Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
WDR34

Plasmid

#132526

Purpose

Human WDR34, codon optimised for Sf9 expression.

Depositor

Andrew Carter

Article

Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

Insert

WDR34 (WDR34 Human)

Use

Tags

Expression

Insect

Mutation

Codon optimised for Sf9 expression

Promoter

Available since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
TCTEX1D2

Plasmid

#132529

Purpose

Human TCTEC-1D2, codon optimised for Sf9 expression.

Depositor

Andrew Carter

Article

Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

Insert

TXCTEX1D2 (TCTEX1D2 Human)

Use

Tags

Expression

Insect

Mutation

Codon optimised for Sf9 expression

Promoter

Available since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
WDR60

Plasmid

#132525

Purpose

Human WDR60, codon optimised for Sf9 expression.

Depositor

Andrew Carter

Article

Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

Insert

WDR60 (WDR60 Human)

Use

Tags

Expression

Insect

Mutation

Codon optimised for Sf9 expression

Promoter

Available since

Oct. 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 2801 - 2840 of 13524 results

Results per page:

Sort results by: