Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 30,195 results for: REP

Clear keywords
Showing: 1401 - 1420 of 30195 results

  5. ACE Reporter (pLenti-CMV-mCherry (+43) -T2A-eGFP)

    Plasmid
    #109428
    Purpose
    A lentiviral backbone expressing mCherry with a 43 basepair insert and eGFP off of a CMV promoter. A reporter for both Cas9 nuclease and APOBEC-mediated base-editing activity.
    Depositor
    Reuben Harris
    Article
    St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994
    Insert
    mCherry T2A GFP
    Use
    Lentiviral
    Mutation
    Inserted 43 base pairs into mCherry to create rep…
    Promoter
    CMV
    Available Since
    July 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    109428-plasmid-map-sequence-id-212894

    Enlarge

  6. pLVX-2xFLAG-2xSTREP-CCND1(P287A)-IRES-mCherry

    Plasmid
    #172637
    Purpose
    Lentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D1(P287A) and mCherry in mammalian cells
    Depositor
    Michele Pagano
    Article
    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:
    Insert
    CCND1 (CCND1 Human)
    Use
    Lentiviral
    Tags
    2xFLAG-2xSTREP
    Expression
    Mammalian
    Mutation
    Pro287Ala
    Promoter
    CMV
    Available Since
    Aug. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    172637-plasmid-map-sequence-id-340160

    Enlarge

  7. pLVX-2xFLAG-2xSTREP-CCND1(T286A)-IRES-mCherry

    Plasmid
    #172639
    Purpose
    Lentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D1(T286A) and mCherry in mammalian cells
    Depositor
    Michele Pagano
    Article
    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:
    Insert
    CCND1 (CCND1 Human)
    Use
    Lentiviral
    Tags
    2xFLAG-2xSTREP
    Expression
    Mammalian
    Mutation
    Thr286Ala
    Promoter
    CMV
    Available Since
    Aug. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    172639-plasmid-map-sequence-id-340169

    Enlarge

  10. pLVX-2xFLAG-2xSTREP-CCND1(P287S)-IRES-mCherry

    Plasmid
    #172635
    Purpose
    Lentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D1(P287S) and mCherry in mammalian cells
    Depositor
    Michele Pagano
    Article
    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:
    Insert
    CCND1 (CCND1 Human)
    Use
    Lentiviral
    Tags
    2xFLAG-2xSTREP
    Expression
    Mammalian
    Mutation
    Pro287Ser
    Promoter
    CMV
    Available Since
    Aug. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    172635-plasmid-map-sequence-id-340162

    Enlarge

  11. pB6-Hipp11-td-sfGFP dre reporter-FNF-DTA

    Plasmid
    #139513
    Purpose
    B6J Hipp11-pCAG-rox-FNF-bGHpA-3xSV40pA-rox-td-sfGFP-WPRE-bpA allele targeting vector, low copy number
    Depositor
    Mario Capecchi
    Insert
    CAG rox stop rox td-sfGFP dre reporter
    Use
    Mouse Targeting; Dre/rox
    Expression
    Mammalian
    Available Since
    Nov. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    139513-plasmid-map-sequence-id-317907

    Enlarge

  15. pPICZ A-SLC(2-88)-MBP-His+MFN2-Strep (SB262)

    Plasmid
    #227611
    Purpose
    Inducible coexpression of His-tagged SLC25A46 truncated to its N-terminal region (2-88) fused to MBP and MFN2-Strep in Pichia pastoris
    Depositor
    Luke Chao
    Article
    Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10
    Inserts
    SLC25A46 (SLC25A46 Human)
    MFN2 (MFN2 Human)
    Tags
    MBP, 10xHis and Twin-StrepTag
    Expression
    Yeast
    Mutation
    contains aa 2-88 only
    Promoter
    AOX1
    Available Since
    Dec. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    227611-plasmid-map-sequence-id-455570

    Enlarge

  16. pPICZ A-SLC-His+MFN2[∆TM,∆HB2]-Strep (SB261)

    Plasmid
    #227610
    Purpose
    Inducible coexpression of His-tagged SLC25A46 and Strep-tagged MFN2 lacking its transmembrane region and helical bundle 2 (HB2) in Pichia pastoris
    Depositor
    Luke Chao
    Article
    Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10
    Inserts
    SLC25A46 (SLC25A46 Human)
    MFN2 (MFN2 Human)
    Tags
    10xHis and Twin-StrepTag
    Expression
    Yeast
    Mutation
    contains aa 2-400, 706-757 [TM and HB2 deleted]
    Promoter
    AOX1
    Available Since
    Dec. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    227610-plasmid-map-sequence-id-455569

    Enlarge

  17. pET28-RPL3-191~307-mEGFP(A206K)-TwStrep

    Plasmid
    #208626
    Purpose
    for E. coli expression of truncated human RPL3 (ENST00000216146), aa191~307, fused with mEGFP(A206K) and Twin Strep tag
    Depositor
    Quanfu Ma
    Insert
    human RPL3 (ENST00000216146) aa 191~307 (RPL3 Human)
    Tags
    6xHis, Twin Strep, and mEGFP(A206K)
    Expression
    Bacterial
    Mutation
    only aa 191~307, other residues not included
    Promoter
    T7
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208626-plasmid-map-sequence-id-415912

    Enlarge

  18. pET28-RPL3-299~398-mEGFP(A206K)-TwStrep

    Plasmid
    #208627
    Purpose
    for E. coli expression of truncated human RPL3 (ENST00000216146), aa299~398, fused with mEGFP(A206K) and Twin Strep tag
    Depositor
    Quanfu Ma
    Insert
    human RPL3 (ENST00000216146) aa 299~398 (RPL3 Human)
    Tags
    6xHis, Twin Strep, and mEGFP(A206K)
    Expression
    Bacterial
    Mutation
    only aa 299~398, other residues not included
    Promoter
    T7
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208627-plasmid-map-sequence-id-415915

    Enlarge

  19. pET28-RPL3-212~285-mEGFP(A206K)-TwStrep

    Plasmid
    #208629
    Purpose
    for E. coli expression of truncated human RPL3 (ENST00000216146), aa212~285, fused with mEGFP(A206K) and Twin Strep tag
    Depositor
    Quanfu Ma
    Insert
    human RPL3 (ENST00000216146) aa 212~285 (RPL3 Human)
    Tags
    6xHis, Twin Strep, and mEGFP(A206K)
    Expression
    Bacterial
    Mutation
    only aa 212~285, other residues not included
    Promoter
    T7
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208629-plasmid-map-sequence-id-415924

    Enlarge

  20. pGL3-luciferase-reporter-CHAF1B-SVA-del-VNTR

    Plasmid
    #202830
    Purpose
    pGL3 luciferase reporter with CHAF1B-SVA-del-VNTR sequence cloned upstream of SV40 promoter to test effects of SVA-del-VNTR insertion on luciferase expression
    Depositor
    Matthew Anderson
    Article
    Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:
    Insert
    CHAF1B intronic SVA del-VNTR sequence
    Use
    Luciferase
    Expression
    Mammalian
    Promoter
    SV40
    Available Since
    July 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    202830-plasmid-map-sequence-id-399708

    Enlarge

Showing: 1401 - 1420 of 30195 results