We narrowed to 7,221 results for: ALP

1. Lenti-DIO-P-Ef1a-mCherry-T2A-RPL22-1xV5

   Plasmid

   #170328

   Purpose

   Double-floxed inverse Orientation V5 and mCherry tagged RPL22

   Depositor

   Hemali Phatnani

   Article

   Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

   Insert

   RPL22 (RPL22 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   None

   Promoter

   Available since

   May 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. CD109-bio-His

   Plasmid

   #51862

   Purpose

   Expresses full-length CD109 antigen precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   CD109 (CD109 Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. PRNP-bio-His

   Plasmid

   #51811

   Purpose

   Expresses full-length Major prion protein precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   PRNP (PRNP Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGEX-CDC27 wt

   Plasmid

   #31673

   Depositor

   Anne Brunet

   Article

   Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

   Insert

   cell division cycle 27 homolog (CDC27 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 11, 2012

   Availability

   Academic Institutions and Nonprofits only

5. pAAV-EF1_Cre

   Plasmid

   #201198

   Purpose

   AAV vector for cre expression under EF1 promoter

   Depositor

   Tetsuo Yamamori

   Article

   Watakabe et al Neuron. 2023 May 9:S0896-6273(23)00338-0

   Inserts

   cre
   EF1alpha promoter
   

   Use

   AAV

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   nuclear targeting cre

   Promoter

   N/A and hEF1alpha promoter

   Available since

   July 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. ICAM5-bio-His

   Plasmid

   #51759

   Purpose

   Expresses full-length Intercellular adhesion molecule 5 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   ICAM5 (ICAM5 Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. TFORF2597

   Plasmid

   #142963

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   RORA (RORA Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. CD226-bio-His

   Plasmid

   #51646

   Purpose

   Expresses full-length CD226 antigen precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   CD226 (CD226 Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. TFORF0499

   Plasmid

   #143881

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   PPARGC1A (PPARGC1A Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCDH-EF1a-hLMX1A-T2A-copGFP

    Plasmid

    #170444

    Purpose

    Lentiviral vector expressing human LMX1A (with silent mutation) with copGFP reporter gene, under control of EF1alpha promoter.

    Depositor

    Sang-Hun Lee

    Article

    Wulansari et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabb

    Insert

    human LMX1A
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. TFORF2049

    Plasmid

    #143866

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    PURA (PURA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Human b4 nicotinic receptor subunit EM construct

    Plasmid

    #163961

    Purpose

    pEZT-BM vector with beta4 gene after CMV promoter, published EM construct

    Depositor

    Ryan Hibbs

    Article

    Gharpure et al Neuron. 2019 Nov 6;104(3):501-511.e6. do

    Insert

    Human beta4 nicotinic acetylcholine receptor subunit EM construct
    

    Use

    Baculovirus (bacmam) production and mammalian exp…

    Tags

    StrepII tag and apocytochrome b(562)RIL (BRIL)

    Expression

    Mammalian

    Mutation

    Pro341-Ser395 replaced with apocytochrome b(562)R…

    Promoter

    CMV

    Available since

    Feb. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. ECE1-His-bio

    Plasmid

    #53361

    Purpose

    Expresses full-length Endothelin converting enzyme 1 ectodomain in mammalian cells. N-terminal His tag, biotinylation sequence and rat Cd4d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    ECE1 (ECE1 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGEM-HSPA5P

    Plasmid

    #44150

    Purpose

    a fragment containing 429 bp of the human HSPA5 promoter (-282 to +147)

    Depositor

    Joan Conaway

    Article

    Sela et al J Biol Chem. 2012 Jun 29;287(27):23035-4

    Insert

    HSPA5 promoter (HSPA5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

15. CD8-pTagRFP657-LOVpep (T406A, T407A, I532A)

    Plasmid

    #100742

    Purpose

    To express a plasma membrane "anchor" in mammalian cells to hot-wire endocytosis

    Depositor

    Stephen Royle

    Article

    Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

    Insert

    CD8A (CD8A Human)
    

    Use

    Tags

    LOVpep and tagRFP657

    Expression

    Mammalian

    Mutation

    Truncated

    Promoter

    CMV

    Available since

    Sept. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGEX-6P1-hPXR-LBD

    Plasmid

    #67769

    Purpose

    Expression GST-hPXR-LBD in bacteria

    Depositor

    Sridhar Mani

    Article

    Li et al J Biol Chem. 2013 May 10;288(19):13655-6

    Insert

    human pregnane X receptor (PXR) ligand binding domain (NR1I2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Contains amino acids 107 to 434 of human PXR

    Promoter

    Tac

    Available since

    July 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLV-shEsr2

    Plasmid

    #120722

    Purpose

    Lentiviral vector that expresses GFP and an shRNA targeting Esr2 (in pLL3.7).

    Depositor

    Amy Lasek

    Article

    Vandegrift et al J Neurosci. 2020 Jul 1;40(27):5196-5207.

    Insert

    Esr2 (Esr2 Mouse)
    

    Use

    Lentiviral and RNAi

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    mouse U6

    Available since

    July 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRC/CMV-TYK2K930R-VSV

    Plasmid

    #139346

    Purpose

    Expression of kinase-dead TYK2-K930R protein

    Depositor

    Sandra Pellegrini

    Article

    Gauzzi et al J Biol Chem. 1996 Aug 23;271(34):20494-5

    Insert

    TYK2 cDNA with 267nt of 5' UTR (TYK2 Human)
    

    Use

    Tags

    VSV

    Expression

    Mammalian

    Mutation

    V362F-K930R

    Promoter

    CMV

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBabe puro-HA-PIK3CA(H1047R;F486S;del)

    Plasmid

    #33255

    Depositor

    Thomas Roberts

    Article

    Ilic et al Proc Natl Acad Sci U S A. 2011 Sep 13;10

    Insert

    PIK3CA (PIK3CA Human)
    

    Use

    Retroviral

    Tags

    Hemagglutinin (HA)

    Expression

    Mammalian

    Mutation

    changed histidine 1047 to arginine and phenylalan…

    Promoter

    SV40

    Available since

    Jan. 4, 2012

    Availability

    Academic Institutions and Nonprofits only

20. F2R-bio-His

    Plasmid

    #51863

    Purpose

    Expresses full-length Proteinase-activated receptor 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    F2R (F2R Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCR3-FLAG-Gephyrin GC

    Plasmid

    #68818

    Purpose

    Flag tagged mammalian expression of GC domain

    Depositor

    Shiva Tyagarajan

    Article

    Tyagarajan et al J Biol Chem. 2013 Apr 5;288(14):9634-47.

    Insert

    Gephyrin (Gphn Rat)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    NIL

    Promoter

    CMV

    Available since

    Aug. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. mCherry-FKBP-TPD54(R159E)

    Plasmid

    #172453

    Purpose

    Expression of Tumor Protein D52 like 2 (TPD54/TPD52L2) with N-terminal mCherry-FKBP tag R159E mutant

    Depositor

    Stephen Royle

    Article

    Larocque et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

    Insert

    TPD54 (TPD52L2 Human)
    

    Use

    Tags

    mCherry-FKBP

    Expression

    Mammalian

    Mutation

    changed Arginine 159 to Glutamic Acid

    Promoter

    CMV

    Available since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. TTMP-His-bio

    Plasmid

    #52335

    Purpose

    Expresses full-length TPA-induced transmembrane protein ectodomain in mammalian cells. N-terminal His tag, biotinylation sequence and rat Cd4d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    TTMP (C3orf52 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA6-N-3XFLAG-Fzd6

    Plasmid

    #123588

    Depositor

    Bruce Beutler

    Article

    Choi et al Science. 2019 May 10;364(6440). pii: 364

    Insert

    frizzled class receptor 6 (Fzd6 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. FB009

    Plasmid

    #119707

    Purpose

    Transcriptional Unit (TU) for geneticin (G418) resistance in fungi obtained by combining FB001+FB005+FB002 into pDGB3alpha2. According to FungalBraid/GoldenBraid modular DNA assembly for ATMT

    Depositor

    Jose Marcos

    Article

    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d

    Insert

    PtrpC::nptII::Ttub
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. VP16-ER beta (short)

    Plasmid

    #11353

    Depositor

    Donald McDonnell

    Article

    Chang et al Mol Cell Biol. 1999 Dec . 19(12):8226-39

    Insert

    ER beta short (ESR2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 10, 2006

    Availability

    Academic Institutions and Nonprofits only

27. SNS-GFP

    Plasmid

    #163662

    Purpose

    Expresses a TNF and ST6GAL1 swapped chimera with the cytosolic tail from ST6GAL1, TMD from TNF and luminal domain from ST6GAL1 in mammalian cells

    Depositor

    Lei Lu

    Article

    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

    Insert

    ST6 beta-galactoside alpha-2,6-sialyltransferase 1 and tumor necrosis factor (ST6GAL1, TNF Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    This chimera contains the cytosolic tail from ST,…

    Promoter

    Available since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. INSR-bio-His

    Plasmid

    #53341

    Purpose

    Expresses full-length Insulin receptor precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    INSR (INSR Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. FCGR2a-bio-His

    Plasmid

    #51754

    Purpose

    Expresses full-length Low affinity immunoglobulin gamma Fc region receptor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    FCGR2a (FCGR2A Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLVX neo ACTN1-GFP, Y246E

    Plasmid

    #138294

    Purpose

    Mammalian Expression, Lentiviral. Expression of human alpha-actinin-1-GFP, Y246E fusion protein

    Depositor

    Gaudenz Danuser

    Article

    Park et al Nature. 2020 Feb 12. pii: 10.1038/s41586

    Insert

    ACTN1 (ACTN1 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Y246E

    Promoter

    CMV

    Available since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pPKm-230

    Plasmid

    #90499

    Purpose

    pSIN - EF-1alpha - PIF3 - MTAD - IRES - PhyB - GBD, dual vector of PIF3-MTAD under EF-1 alpha promoter and PhyB-DBD under IRES promoter; See growth conditions below.

    Depositor

    Todd Coleman

    Article

    Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

    Insert

    mito-tFD and mito-tFNR
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pUHD-HA-AML1-ETO

    Plasmid

    #12430

    Depositor

    Dong-Er Zhang

    Article

    Rhoades et al Blood. 2000 Sep 15. 96(6):2108-15.

    Insert

    AML1-ETO (RUNX1, RUNX1T1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    BstXI-SacII fragment in pUHD-AML1-ETO replaced wi…

    Promoter

    Available since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

33. pJO427

    Plasmid

    #111139

    Purpose

    URT-type vector for Glu-mIκBα expression in Mammalian cells

    Depositor

    Alexander Varshavsky

    Article

    Piatkov et al Proc Natl Acad Sci U S A. 2014 Mar 4;111

    Insert

    flag-lacZ25-119-DHFR-UbK48R-Glu51-mIκBα (Ikbkb Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pTER GAMMA-TUBULIN shRNA Human

    Plasmid

    #87955

    Purpose

    reduced the expression of gamma-tubulin in human cells

    Depositor

    Maria Alvarado-Kristensson, Ana Carrera

    Article

    Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

    Insert

    sh gamma-tubulin (TUBG1 Human)
    

    Use

    Tags

    No-tag

    Expression

    Mammalian

    Mutation

    The protein is a sh-gamma-tubulin resistant gene.…

    Promoter

    Available since

    Oct. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pME-GcAMP5

    Plasmid

    #126569

    Purpose

    Multisite gateway middle entry clone containing zebrafish calcium reporter GcAMP5. Parton lab clone ANR.

    Depositor

    Rob Parton

    Article

    Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

    Insert

    GcAMP5
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pHH0103_ARHGEF6-1/1

    Plasmid

    #91515

    Purpose

    Protein expression and purification of human SH3 domain construct ARHGEF6-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    ARHGEF6-1/1 (ARHGEF6 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCMVTNT-EPHX1

    Plasmid

    #53114

    Purpose

    In vitro translation of microsomal epoxide hydrolase

    Depositor

    Xu Lin

    Article

    Chen et al BMC Cancer. 2014 Apr 23;14:282. doi: 10.

    Insert

    EPHX1 (EPHX1 Human)
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    May 7, 2014

    Availability

    Academic Institutions and Nonprofits only

38. integrin beta6 777T pcDNA1 neo

    Plasmid

    #13582

    Depositor

    Dean Sheppard

    Article

    Morris et al Nature. 2003 Mar 13. 422(6928):169-73.

    Insert

    integrin beta 6 777T (ITGB6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    777T. Lacks last 11 amino acids of cytoplasmic do…

    Promoter

    Available since

    Jan. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pRC/CMV-TYK2K930R-FF-VSV

    Plasmid

    #139348

    Purpose

    Expression of kinase-dead TYK2-K930R missing the two tyrosines at the activation loop

    Depositor

    Sandra Pellegrini

    Article

    Gauzzi et al J Biol Chem. 1996 Aug 23;271(34):20494-5

    Insert

    TYK2 cDNA with 267nt of 5' UTR (TYK2 Human)
    

    Use

    Tags

    VSV

    Expression

    Mammalian

    Mutation

    V362F-K930R-Y1054F-Y1055F

    Promoter

    CMV

    Available since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. APCS-bio-His

    Plasmid

    #53424

    Purpose

    Expresses full-length Serum amyloid P-component precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    APCS (APCS Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pRC/CMV-TYK21054-5FF-VSV

    Plasmid

    #139347

    Purpose

    Expression of TYK2 protein mutated in the two tyrosines (Y1054F/Y1055F) of the activation loop

    Depositor

    Sandra Pellegrini

    Article

    Gauzzi et al J Biol Chem. 1996 Aug 23;271(34):20494-5

    Insert

    TYK2 cDNA with 267nt of 5' UTR (TYK2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    V362F-Y1054F-Y1055F

    Promoter

    CMV

    Available since

    June 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pCR3-FLAG-Gephyrin G

    Plasmid

    #68817

    Purpose

    Geph G domain expression in mamalian cells

    Depositor

    Shiva Tyagarajan

    Article

    Tyagarajan et al J Biol Chem. 2013 Apr 5;288(14):9634-47.

    Insert

    Gephyrin (Gphn Rat)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    NIL

    Promoter

    CMV

    Available since

    Aug. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

43. pJO428

    Plasmid

    #111141

    Purpose

    URT-type vector for Val-mIκBα expression in Mammalian cells

    Depositor

    Alexander Varshavsky

    Article

    Piatkov et al Proc Natl Acad Sci U S A. 2014 Mar 4;111

    Insert

    flag-lacZ25-119-DHFR-UbK48R-Val51-mIκBα (Ikbkb Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pAAV_Donor/Reporter-COL4A5

    Plasmid

    #130280

    Purpose

    COL4A5 mutation-specific sgRNA under the control of U6 promoter; mCherry-sgRNA target-(out of frame)EGFP expression cassette; COL4A5 donor template

    Depositor

    Silvestro Conticello

    Article

    Daga et al Eur J Hum Genet. 2019 Nov 21. pii: 10.10

    Insert

    mCherry-eGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. CD8-dCherry-FRB

    Plasmid

    #100739

    Purpose

    To express a plasma membrane "anchor" in mammalian cells to hot-wire endocytosis (dark mCherry K70N)

    Depositor

    Stephen Royle

    Article

    Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

    Insert

    CD8A (CD8A Human)
    

    Use

    Tags

    FRB and dCherry

    Expression

    Mammalian

    Mutation

    Dark mCherry (K70N) non-fluorescent

    Promoter

    CMV

    Available since

    Sept. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. CALCR-bio-His

    Plasmid

    #53412

    Purpose

    Expresses full-length Calcitonin receptor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    CALCR (CALCR Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pCDNA3.1-nAChr-beta3

    Plasmid

    #48811

    Purpose

    This report is the first to directly measure nAChR subunit stoichiometry using FRET and plasma membrane localization of α6- and β3-containing receptors using TIRF.

    Depositor

    Jerry Stitzel, Henry Lester

    Article

    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

    Insert

    nAChr Beta 3 subunit, isoform 1 (Chrnb3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    *

    Promoter

    CMV

    Available since

    Sept. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. EBFP2-UtrCH

    Plasmid

    #124656

    Purpose

    F-actin marker. EBFP2 is fused to the calponin homology domain of utrophin (UtrCH).

    Depositor

    Cheng-han Yu

    Article

    Zhang et al Mol Biol Cell. 2019 Mar 1;30(5):622-635.

    Insert

    Utrophin (UTRN Human)
    

    Use

    Tags

    EBFP2

    Expression

    Mammalian

    Mutation

    see depositor comments below

    Promoter

    Available since

    May 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. F2RL3-bio-His

    Plasmid

    #51864

    Purpose

    Expresses full-length Proteinase-activated receptor 4 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    F2RL3 (F2RL3 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. GGH-bio-His

    Plasmid

    #52066

    Purpose

    Expresses full-length Gamma-glutamyl hydrolase precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    GGH (GGH Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge