We narrowed to 6,904 results for: CAD
-
Plasmid#80641PurposeResidues 1-359 of ALIX; Bacterial Expression Vector; N-term His-tag; Sundquist Lab Internal ID: WISP11-428DepositorInsertALIX Bro1, residues 1-359 (PDCD6IP Human)
UseTagsHis TagExpressionBacterialMutationResidues 1-359PromoterT7Available sinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSLiP-G1
Plasmid#188963PurposeL-Rhamnose inducible catalytically inactive Cas9 with sgRNA for bacterial gene knockdownDepositorInsertsdCas9
sgRNA: atcggtcgcattgttttccactagg
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPrhaBADAvailable sinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
RNA polymerase beta prime subunit FLAG
Plasmid#102337PurposeTargeting plasmids for replacing the S. elongatus PCC 7942 genomic copy of Synpcc7942_1524 (Beta prime subunit of RNA polymerase) with a C-terminal FLAG tagged variant.DepositorInsertRNAP Beta Prime Subunit with C-terminal FLAG TAG
UseTags3x FLAG with 3X GSGS linkerExpressionMutationPromoterAvailable sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOPS0385
Plasmid#133235PurposeReporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.DepositorInsertconstructed with NoP (pOGG113), gusA (pOGG083) and T-pharma (pOGG003)
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceApril 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOPS0386
Plasmid#133236PurposeReporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.DepositorInsertconstructed with NoP (pOGG113), celB (pOGG050) and T-pharma (pOGG003)
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceApril 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOPS0383
Plasmid#133233PurposeReporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.DepositorInsertconstructed with NoP (pOGG113), sfGFP (pOGG037) and T-pharma (pOGG003)
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceApril 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-H2-Ld
Plasmid#135519PurposeMammalian expression of myc-tagged H2-LdDepositorInsertH2-Ld
UseTagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-HA-MEKK1-1-1493
Plasmid#21632DepositorInsertmitogen-activated protein kinase kinase kinase 1 (Map3k1 Rat)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceFeb. 8, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_PKA-CαN (K29,30A)-mVenus
Plasmid#168485PurposeMammalian expression of the K29,30A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus.DepositorInsertthe K29,30A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBSSK p85 alpha (delta SH3)
Plasmid#13430DepositorInsertp85 alpha (delta SH3) (PIK3R1 Human)
UseTagsFLAGExpressionBacterialMutationSH3 domain replaced by flag tag.PromoterAvailable sinceNov. 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Fah_W
Plasmid#163087Purposeexpressing mouse wild type FAH in mammalian cellsDepositorInsertFah_W
UseTagsMyc-HisExpressionMammalianMutationPromoterCMVAvailable sinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBW300ParsR-Amp32T
Plasmid#78652PurposepSB3K3 carrying araC-PBAD-30hrpV-t-J101-32arsR-t-ParsR-30hrpR-32hrpS-t-hrpL-30gfp-tDepositorInsertaraC-PBAD-30hrpV-t-J101-32arsR-t-ParsR-
UseSynthetic BiologyTagsExpressionMutationPromotersee insertAvailable sinceJuly 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBABE-hygro NM23 H1 WT
Plasmid#11364DepositorInsertNM23 H1 (NME1 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET28a-UVRAG CC
Plasmid#24400DepositorInsertUVRAG (UVRAG Human)
UseTagsHisExpressionBacterialMutationContains on the CC domain, amino acids 200-270PromoterAvailable sinceApril 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pACUH-GFP1-10 mitochondrial matrix
Plasmid#172063PurposeUAS vector to express GFP1-10 in the mitochondrial matrixDepositorInsertGFP1-10 mitochondrial matrix
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
MC177VSG221RNAi
Plasmid#59730PurposeFor use in trypanosome cell lines already expressing T7 polymerase and Tet-repressor. Tetracycline induction results in the production of dsRNA targeting VSG221.DepositorInsertVSG221 RNAi fragment
UseRNAiTagsExpressionMutationPromoteropposing T7 promotersAvailable sinceAug. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPRIME-TREX-GFP-FF3
Plasmid#11667Purpose3rd generation lentiviral transfer vector. pPRIME cloning plasmid with a Tet-responsive promoter (TREX) controlling expression of GFP and miR30-based shRNA targeting firefly luciferaseDepositorInsertFF3
UseLentiviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceMarch 1, 2007AvailabilityAcademic Institutions and Nonprofits only -
pG4-E6s-luc
Plasmid#46324DepositorInsert5xGal4
UseLuciferaseTagsE boxes and luciferaseExpressionMammalianMutationfive copies of a multimerized GAL4 (5×GAL4) site …PromoterAvailable sinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBacPAK8-ElonginB
Plasmid#29504DepositorInsertElongin B (ELOB Human)
UseTagsExpressionInsectMutationPromoterAvailable sinceApril 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
LifeAct-TagBFP-DiB-RM
Plasmid#168782PurposeActin filament reporter, LifeAct, fused with blue fluorescent protein TagBFP and fluorogen activating protein (FAP) DiB-RMDepositorInsertLifeAct-TagBFP-DiB-RM
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRRG227-Wnt1
Plasmid#99073PurposedsRNA and riboprobe synthesis for Schmidtea mediterranea Wnt1 (also known as WntP-1)DepositorInsertWnt1 (also known as WntP-1) in situ probe
UseT/a cloning vector for dsrna generation and the g…TagsExpressionMutationPromoterAvailable sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAV-tLuc
Plasmid#107552PurposetLuc in AAV backboneDepositorInsertLuciferase
UseAAVTagsExpressionMutationPromoterAvailable sinceJuly 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGST-Fah_W
Plasmid#163089PurposeExpressing mouse wildtype FAH in bacterial cellsDepositorInsertFah_W
UseTagsGSTExpressionBacterialMutationPromoterT7Available sinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTRIPZ-DoxOn-shCUX1-5150
Plasmid#90469PurposeLentiviral vector expressing a doxycycline-inducible shRNA against human CUX1 sequence starting at 5150 of M74099DepositorInsertTGCTGTTGACAGTGAGCGTCAGAGCGATAATACACTATTATAGTGAAGCC
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7 shRNA against CRTC3
Plasmid#72712PurposeLentiviral expression of shRNA against CRTC3 (3rd generation)DepositorInsertCRTC3
UseLentiviralTagsCMV-EGFPExpressionMammalianMutationPromoterU6Available sinceFeb. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV4-Flag-mIL-17RA/YFP.Hygro
Plasmid#46862Purposeencodes Flag-tagged mouse IL-17RA fused to Yellow Fl. ProteinDepositorInsertIL-17RA-YFP
UseTagsFlag/IL-3 signal sequence and fused to CFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
-
pBSSK p85 alpha (delta BH)
Plasmid#13431DepositorInsertp85 alpha (delta BH) (PIK3R1 Human)
UseTagsFLAGExpressionBacterialMutationBH domain replaced by flag tag.PromoterAvailable sinceNov. 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
PumU
Plasmid#87322PurposePumHD w/each unit mutated to bind U (as in Fig. 1C; unit 6 is the Pumby for U)DepositorInsertPumilio protein mutant (PUM1 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationall 8 units mutated to bind UPromoterUBCAvailable sinceJune 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
PumG
Plasmid#87320PurposePumHD w/each unit mutated to bind G (as in Fig. 1C; unit 6 is the Pumby for G)DepositorInsertPumilio protein mutant (PUM1 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationall 8 units mutated to bind GPromoterUBCAvailable sinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
PumA
Plasmid#87319PurposePumHD w/each unit mutated to bind A (as in Fig. 1C; unit 6 is the Pumby for A)DepositorInsertPumilio protein mutant (PUM1 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationall 8 units mutated to bind APromoterUBCAvailable sinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP eIF2beta
Plasmid#45902DepositorInserteIF2S2 (Eif2s2 Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
GAL 103Q+ProCFPp303
Plasmid#15575DepositorInserthtt103Q + Pro (HTT Human)
UseTagsCFPExpressionYeastMutationProline-rich region presentPromoterAvailable sinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pC1300_pUB10_pco-nCASphi_E9t_V2_U6_AtPDS3_gRNA10
Plasmid#197965PurposeT-DNA binary vector to express pco-nCasphi driven by UBQ10 gene promoter, and the AtPDS3 guide RNA 10 driven by U6 promoter.DepositorInsertspco-nCasphi-2
AtPDS3 gRNA10
UseCRISPRTagsExpressionPlantMutationPromoterAtU6-26 and pUBQ10Available sinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
-
deltaMyc-HA
Plasmid#16556DepositorInsertc-MYC (MYC Human)
UseTagsHAExpressionMammalianMutationDeletion of aa 40-178PromoterAvailable sinceJuly 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
pRRG199-bmp4
Plasmid#99076PurposedsRNA and riboprobe synthesis for Schmidtea mediterranea Bmp4 (also known as bmp)DepositorInsertBmp4 (also known as bmp) in situ probe
UseT/a cloning vector for dsrna generation and the g…TagsExpressionMutationPromoterAvailable sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmDCP1_327-372-GST-V5His6_H
Plasmid#146503PurposeInsect Expression of DmDCP1_327-372DepositorInsertDmDCP1_327-372 (Dcp1 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6 MCV LTco S147A
Plasmid#112192PurposeExpression of MCPyV LT mutant (Ser 147 to Ala)DepositorInsertMCPyV LT codon optimized
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET41a iGluf
Plasmid#119833PurposeProduction of recombinant iGluSnFR E25D fast variant, a single-wavelength glutamate indicator, in E. coliDepositorInsertiGluf
UseTagsGSTExpressionBacterialMutationE25DPromoterT7Available sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBacPAK8-ElonginC
Plasmid#29505DepositorInsertElongin C (ELOC Human)
UseTagsExpressionInsectMutationPromoterAvailable sinceApril 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pTRIPZ shCUX1-5328
Plasmid#100815PurposeLentiviral vector expressing a doxycycline-inducible short hairpin RNA against CUX1 sequence starting at 5328 of M74099DepositorInsertTGCTGTTGACAGTGAGCGTCTTCTCGTTTGAAACTTTGAATAGTGAAGCCA
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSU314
Plasmid#169696PurposeTemplate plasmid for generating C-terminal hemagglutinin (1xHA) epitope tag fusions to bacterial genes by "Lambda red" recombineering.DepositorInsert1xHA epitope tag adjacent to FRT-flanked cat (CamR) gene
UseTags1xHA tagExpressionBacterialMutationPromoterAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7 shRNA against CRTC1
Plasmid#72710PurposeLentiviral expression of shRNA against CRTC1 (3rd generation)DepositorInsertCRTC1
UseLentiviralTagsCMV-EGFPExpressionMammalianMutationPromoterU6Available sinceFeb. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLL7.0: Venus-oLID-Mito (From ActA)
Plasmid#60414PurposeMammalian Expression of Venus-oLID-MitoDepositorInsertVenus-oLID-Mito
UseCre/Lox and LentiviralTagsVenusExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSU312
Plasmid#169694PurposeTemplate plasmid for generating C-terminal 1xFLAG epitope tag fusions to bacterial genes by "Lambda red" recombineering.DepositorInsert1xFLAG epitope tag adjacent to FRT-flanked aph (KanR) gene
UseTags1xFLAG tagExpressionBacterialMutationPromoterAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSU313
Plasmid#169695PurposeTemplate plasmid for generating C-terminal 1xFLAG epitope tag fusions to bacterial genes by "Lambda red" recombineering.DepositorInsert1xFLAG epitope tag adjacent to FRT-flanked cat (CamR) gene
UseTags1xFLAG tagExpressionBacterialMutationPromoterAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUHG10.3-TetO-Rs1
Plasmid#16313DepositorInserth5HT4b Serotonin Receptor (Rs1 D100A RASSL) (RS1 Human)
UseTagsFLAGExpressionMammalianMutationAdded FLAG tag; Changed Aspartic Acid 100 to Alan…PromoterAvailable sinceJan. 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
p6611 MSCV-IP N-HAonly UBOX5
Plasmid#35010DepositorInsertUBOX5 (Ubox5 Human)
UseRetroviralTagsHAExpressionMammalianMutationPromoterMSCV LTRAvailable sinceMarch 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET11-PC-H6
Plasmid#183777PurposeExpress PC-H6 in E. coli. Purified PC-H6 was used for for generating antibodies in rabbits and for GST-CBP pull-down assays.DepositorInsertPolycomb (Pc Fly)
UseTags6 His tag at the C-terminusExpressionBacterialMutationPromoterT7Available sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only