We narrowed to 4,106 results for: 261

1. LLhE-pHG165

   Plasmid

   #90045

   Purpose

   Constitutive expression of a chimeric LacI:CelR transcription repressor

   Depositor

   Liskin Swint-Kruse

   Article

   Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

   Insert

   LLhE
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Iq

   Available since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. LLhC-pHG165a

   Plasmid

   #90046

   Purpose

   Constitutive expression of a chimeric LacI:CytR transcription repressor

   Depositor

   Liskin Swint-Kruse

   Article

   Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

   Insert

   LLhC
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Iq

   Available since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. FLAG-mPar6a K19A

   Plasmid

   #86149

   Purpose

   FLAG-mPar6a K19A - Does not bind to aPKC

   Depositor

   Senthil Muthuswamy

   Article

   Nolan et al Cancer Res. 2008 Oct 15;68(20):8201-9.

   Insert

   Par6 (Pard6a Mouse)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Lysine to Alanine mutation K19A

   Promoter

   LTR

   Available since

   March 17, 2017

   Availability

   Academic Institutions and Nonprofits only

4. JARID1BA-c539

   Plasmid

   #79643

   Purpose

   Baculovirus expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   JARID1B (KDM5B Human)
   

   Use

   Baculovirus expression

   Tags

   6x His and TEV cleavage site

   Expression

   Mutation

   Promoter

   polyhedrin

   Available since

   June 16, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pSFFV-Cx45

   Plasmid

   #69808

   Purpose

   mammalian expression of chick Cx45

   Depositor

   Richard Veenstra

   Article

   Veenstra et al Circ Res. 1992 Nov;71(5):1277-83.

   Insert

   Cx45 (GJC1 Chicken)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

6. KHBD00442

   Plasmid

   #34545

   Depositor

   Bart Deplancke

   Article

   Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

   Insert

   CG5264 (btn Fly)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 23, 2012

   Availability

   Academic Institutions and Nonprofits only

7. KHBD00224

   Plasmid

   #34372

   Depositor

   Bart Deplancke

   Article

   Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

   Insert

   CG12219 (CG12219 Fly)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 23, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pET-17b_K490clm_E215C

   Plasmid

   #169995

   Purpose

   Kinesin KIF5B K490 cysteine light mutant with E215C mutation for single cysteine labeling

   Depositor

   Ron Vale

   Article

   Niekamp et al Proc Natl Acad Sci U S A. 2019 Mar 5;116

   Insert

   kinesin family member 5B, isoform CRA_a
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   C7S, C65A, C168A, C174S, E215C, C294A, C330S, C42…

   Promoter

   T7

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. PB_tre_dCas9_KRAB

   Plasmid

   #126030

   Purpose

   PiggyBac cargo vector expressing doxycycline inducible dCas9-KRAB fusion

   Depositor

   Mauro Calabrese

   Article

   Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

   Inserts

   dCas9 fusion with KRAB
   Hygromycin resistance
   

   Use

   CRISPR; Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A (catalytically inactive Cas9)

   Promoter

   EF1 alpha and TRE

   Available since

   June 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1-mSTING

    Plasmid

    #214156

    Purpose

    Transient expression of STING

    Depositor

    Nan Yan

    Article

    Dobbs et al Cell Host Microbe. 2015 Aug 12;18(2):157

    Insert

    STING (Sting1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. PB_tre_dCas9_VP160

    Plasmid

    #126031

    Purpose

    PiggyBac cargo vector expressing doxycycline inducible dCas9-VP160 fusion

    Depositor

    Mauro Calabrese

    Article

    Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

    Inserts

    dCas9 fusion with VP160
    Hygromycin resistance
    

    Use

    CRISPR; Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically inactive Cas9)

    Promoter

    EF1 alpha and TRE

    Available since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pmCherry-NLS

    Plasmid

    #39319

    Depositor

    Martin Offterdinger

    Article

    Micutkova et al Int J Cancer. 2012 Apr 1;130(7):1544-57.

    Insert

    mCherry-3xNLS
    

    Use

    Tags

    3xSV40LT NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 26, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. CAGGS-EGFP-NLS-3XFLAG-V5

    Plasmid

    #196090

    Purpose

    Expresses gfp in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tag

    Depositor

    Mauro Calabrese

    Article

    Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

    Insert

    GFP
    

    Use

    Tags

    C-terminal 3xFLAG and C-terminal V5

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CAGGs

    Available since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3-HA-mRipk3

    Plasmid

    #78805

    Purpose

    To overexpress mRipk3 in Mammalian Cells

    Depositor

    Jaewhan Song

    Article

    Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

    Insert

    RIPK3 (Ripk3 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMRX-mSTING-EGFP

    Plasmid

    #214148

    Purpose

    Retroviral transduction of EGFP-tagged STING

    Depositor

    Nan Yan

    Article

    Chu et al Nature. 2021 Aug;596(7873):570-575. doi:

    Insert

    STING (Sting1 Mouse)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Available since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.1-HA-mSTING

    Plasmid

    #214157

    Purpose

    Transient expression of STING

    Depositor

    Nan Yan

    Article

    Chu et al Nature. 2021 Aug;596(7873):570-575. doi:

    Insert

    STING (Sting1 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTagRFP658-N1

    Plasmid

    #178974

    Purpose

    Expresses TagRFP658 in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

    Insert

    TagRFP658
    

    Use

    Tags

    no

    Expression

    Mammalian

    Mutation

    NO

    Promoter

    CMV

    Available since

    Aug. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMXs-mGCN2-FLAG-IP

    Plasmid

    #101794

    Purpose

    Retroviral vector encoding C-terminally FLAG tagged mouse GCN2

    Depositor

    Seiichi Oyadomari

    Article

    Taniuchi et al Sci Rep. 2016 Sep 16;6:32886. doi: 10.10

    Insert

    Gcn2 (Eif2ak4 Mouse)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Intron-Tagging-pX330-Cas9-Blast

    Plasmid

    #159741

    Purpose

    Expresses Cas9-2A-Blast and a sgRNA excising EGFP flanked by a splice acceptor and a splice donor from the Intron-Tagging-EGFP-Donor plasmid.

    Depositor

    Stefan Kubicek

    Article

    Reicher et al Genome Res. 2020 Dec;30(12):1846-1855. d

    Insert

    donor plasmid targeting sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMX-mTAF4b

    Plasmid

    #44345

    Depositor

    Marc Timmers

    Article

    Pijnappel et al Nature. 2013 Mar 28;495(7442):516-9. doi

    Insert

    TAF4b (Taf4b Mouse)
    

    Use

    Retroviral

    Tags

    flag and SBP

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    Oct. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLenti-Puro-sgNT

    Plasmid

    #138681

    Purpose

    Expresses a non-targeting sgRNA and Cas9

    Depositor

    Reuben Shaw

    Article

    Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

    Insert

    sgNT
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. [pSK467] pLJC5 HA-Depdc5

    Plasmid

    #109343

    Purpose

    Lenti-viral expression of HA-tagged wildtype Depdc5

    Depositor

    Kuang Shen, David Sabatini

    Article

    Shen et al Nature. 2018 Apr 5;556(7699):64-69. doi:

    Insert

    Depdc5 (DEPDC5 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLVX-opto-mRipK3

    Plasmid

    #208783

    Purpose

    Inducibly expresses mouse RipK3 fused to light activatable Cry2

    Depositor

    Petr Broz

    Article

    Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

    Insert

    mCherry mRipK3 Cry2 (Ripk3 Mouse)
    

    Use

    Lentiviral

    Tags

    Cry2 and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA-HA-LATS2-KR

    Plasmid

    #33100

    Depositor

    Kunliang Guan

    Article

    Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

    Insert

    lats2 (Lats2 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K655R, Kinase Dead

    Promoter

    CMV

    Available since

    March 27, 2012

    Availability

    Academic Institutions and Nonprofits only

25. pLenti_CamKIIa_mKate2

    Plasmid

    #96942

    Purpose

    To label mature excitatory iPSC-derived human neurons

    Depositor

    Oleksandr Shcheglovitov, Ricardo Dolmetsch

    Article

    Shcheglovitov et al Nature. 2013 Nov 14;503(7475):267-71. do

    Insert

    far-red fluorescent protein
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    E87D (please see depositor comments below)

    Promoter

    CaMKIIa

    Available since

    Aug. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLenti-PGK-Hygro-amp-sgRNA-resistant PBRM1

    Plasmid

    #107407

    Purpose

    Lentiviral expression of gRNA resistant PBRM1

    Depositor

    William Kaelin

    Article

    Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

    Insert

    sg resistant PBRM1 (PBRM1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    sgRNA resistant

    Promoter

    Available since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLenti-Blast-sgNT

    Plasmid

    #138678

    Purpose

    Expresses a Non-targeting sgRNA and Cas9

    Depositor

    Reuben Shaw

    Article

    Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

    Insert

    sgNT
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. [pSK468] pLJC5 Flag-Depdc5

    Plasmid

    #109337

    Purpose

    Lenti-viral expression of Flag-tagged wildtype Depdc5

    Depositor

    Kuang Shen, David Sabatini

    Article

    Shen et al Nature. 2018 Apr 5;556(7699):64-69. doi:

    Insert

    Depdc5 (DEPDC5 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    Available since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. sgPBRM1-1- LentiCRISPRv2

    Plasmid

    #107403

    Purpose

    Lentiviral expression of Cas9 and gRNA targeting PBRM1

    Depositor

    William Kaelin

    Article

    Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

    Insert

    gRNA targeting PBRM1 (PBRM1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. DEL-pHG165a

    Plasmid

    #90064

    Purpose

    LLhG coding region on pHG165a interrupted with a single base-pair deletion, 'no repressor' control plasmid

    Depositor

    Liskin Swint-Kruse

    Article

    Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

    Insert

    DEL
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Iq

    Available since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. sgPBRM1-2- LentiCRISPRv2

    Plasmid

    #107404

    Purpose

    Lentiviral expression of Cas9 and gRNA targeting PBRM1

    Depositor

    William Kaelin

    Article

    Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

    Insert

    gRNA targeting PBRM1 (PBRM1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. p1192-scAAV-U6-sgRNA_Nme2Cas9_Rosa26-CB-PI-AcrIIC3Nme_FLAG_NLS

    Plasmid

    #129530

    Purpose

    Self-complementary AAV vector expressing Nme2Cas9 sgRNA targeting Rosa26 and AcrIIC3Nme

    Depositor

    Erik Sontheimer

    Article

    Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

    Inserts

    codon-optimized AcrIIC3Nme
    sgRNA_Rosa26
    

    Use

    AAV

    Tags

    FLAG/NLS

    Expression

    Mutation

    Promoter

    U6 and cytomegalovirus-enhancer chicken β-actin p…

    Available since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. LacI-11/109-pHG165c

    Plasmid

    #90059

    Purpose

    Dimeric LacI (truncated last 11 amino acids) polymorphism with T at position 109

    Depositor

    Liskin Swint-Kruse

    Article

    Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

    Insert

    LacI-11/109
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    109

    Promoter

    Iq

    Available since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCAG-Bmpr2ΔKinase

    Plasmid

    #163404

    Purpose

    Expresses BMPR-2 lacking the kinase domain in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Bmpr2 (Bmpr2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    The kinase domain (202-500 aa) is deleted

    Promoter

    Available since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCI-MS2V5-PABPC1 M161A/D165K

    Plasmid

    #65809

    Purpose

    expression of PABPC1-MS2 fusion. Mutations abolish binding to eIF4G

    Depositor

    Niels Gehring

    Article

    Fatscher et al RNA. 2014 Oct;20(10):1579-92. doi: 10.12

    Insert

    PABPC1 (PABPC3 Human)
    

    Use

    Tags

    MS2 stem loops and V5

    Expression

    Mammalian

    Mutation

    M161A/D165K

    Promoter

    CMV

    Available since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. Intron-Tagging-pX330-Cas9-mCherry

    Plasmid

    #159742

    Purpose

    Expresses Cas9-2A-mCherry and a sgRNA excising EGFP flanked by a splice acceptor and a splice donor from the Intron-Tagging-EGFP-Donor plasmid.

    Depositor

    Stefan Kubicek

    Article

    Reicher et al Genome Res. 2020 Dec;30(12):1846-1855. d

    Insert

    donor plasmid targeting sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pSECC-sgTom

    Plasmid

    #138661

    Purpose

    Expresses a Tomato-targeting sgRNA, Cas9, and Cre-recombinase

    Depositor

    Reuben Shaw

    Article

    Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

    Insert

    sgTomato
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. p35u4

    Plasmid

    #174786

    Purpose

    Constitutive expression of λCI fused via three alanines to the MS2 coat protein. Useful as RNA-DNA adapter in three-hybrid system

    Depositor

    Katherine Berry

    Article

    Wang et al RNA. 2021 Apr;27(4):513-526. doi: 10.126

    Insert

    MS2 coat protein
    

    Use

    Tags

    λCI

    Expression

    Bacterial

    Mutation

    V30I, A81G, positions 68-80 deleted (VATQTVGGVELP…

    Promoter

    Synthetic Constitutive Promoter

    Available since

    May 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. p1194-scAAV-U6-sgRNA_Nme2Cas9_Rosa26-CB-PI-AcrIIA4_FLAG_NLS

    Plasmid

    #129532

    Purpose

    Self-complementary AAV vector expressing Nme2Cas9 sgRNA targeting Rosa26 and AcrIIA4

    Depositor

    Erik Sontheimer

    Article

    Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

    Inserts

    codon-optimized AcrIIA4
    sgRNA_Rosa26
    

    Use

    AAV

    Tags

    FLAG/NLS

    Expression

    Mutation

    Promoter

    U6 and cytomegalovirus-enhancer chicken β-actin p…

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. shPBRM1#32-Tet-pLKO-puro

    Plasmid

    #107399

    Purpose

    Lentiviral expression of shRNA targeting PBRM

    Depositor

    William Kaelin

    Article

    Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

    Insert

    shRNA targeting PBRM1 (PBRM1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. shPBRM1#131-Tet-pLKO-puro

    Plasmid

    #107400

    Purpose

    Lentiviral expression of shRNA targeting PBRM

    Depositor

    William Kaelin

    Article

    Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

    Insert

    shRNA targeting PBRM1 (PBRM1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. GA-DEVD-GB-NES

    Plasmid

    #61020

    Purpose

    green ddFP based caspase-3 biosensor

    Depositor

    Robert Campbell

    Article

    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

    Insert

    ddGFP A and ddGFP B
    

    Use

    Tags

    HindIII and NES sequence LALKLAGLDIGS placed afte…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

43. Neo1.a-AP-His

    Plasmid

    #71963

    Purpose

    Expresses the extracellular region of the Neogenin 1, isoform a protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Neo1.a (Neo1 Mouse)
    

    Use

    Tags

    AP-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pCI-MS2V5-PABPC1 MLLEmut M161A/D165K

    Plasmid

    #65810

    Purpose

    expression of PABPC1-MS2 fusion, double mutant.

    Depositor

    Niels Gehring

    Article

    Fatscher et al RNA. 2014 Oct;20(10):1579-92. doi: 10.12

    Insert

    PABPC1 (PABPC4 Human)
    

    Use

    Tags

    MS2 stem loops and V5

    Expression

    Mammalian

    Mutation

    MLLEmut M161A/D165K

    Promoter

    CMV

    Available since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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45. pGEMHE-Pom121-mScarlet

    Plasmid

    #171491

    Purpose

    T7 promotor drives in vitro transcription of mScarlet-tagged mouse Pom121 mRNA

    Depositor

    Melina Schuh

    Article

    Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

    Insert

    Pom121 (Pom121 Mouse)
    

    Use

    Pgehme

    Tags

    mScarlet

    Expression

    Mutation

    Promoter

    T7

    Available since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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46. PAmCherry-N1-FRB-Tubbyc

    Plasmid

    #202678

    Purpose

    Photoactivatable and rapamycin recruitable lipid biosensor

    Depositor

    Gerry Hammond

    Article

    Pacheco et al J Cell Biol. 2023 Feb 6;222(2):e20220409

    Insert

    Mus musculus Tub (243-505):MTOR(2021-2113):PAmCherry1 (Tub, Mtor Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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47. shPBRM1 #122-Tet-pLKO-puro

    Plasmid

    #107401

    Purpose

    Lentiviral expression of shRNA targeting PBRM

    Depositor

    William Kaelin

    Article

    Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

    Insert

    shRNA targeting PBRM1 (PBRM1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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48. N163 nLV EF-1a-Hp1a(CS)-Frbx2(Frb+FrbWobb)-V5-PGK-Puro

    Plasmid

    #44195

    Depositor

    Jerry Crabtree

    Article

    Hathaway et al Cell. 2012 Jun 22;149(7):1447-60. doi: 1

    Insert

    Cbx5 (Cbx5 Mouse)
    

    Use

    Lentiviral

    Tags

    Frb, Hu Frb x2(WT Frb+codon Wobble Frb), and V5/e…

    Expression

    Mutation

    N-term chromo domain removed

    Promoter

    EF-1a

    Available since

    June 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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49. pRibo-APEX2m

    Plasmid

    #176842

    Purpose

    Expression of APEX2 from a codon-optimized gene (multi-copy episomal plasmid)

    Depositor

    Jessica Seeliger

    Article

    Ahamed et al Methods Enzymol. 2022;664:267-289. doi:

    Insert

    pRibo-APEX2m
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pRibo, theophilline riboswitch inducible hsp60 pr…

    Available since

    Jan. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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50. pT7-MS2-HA-C1-MBP-DmBam_13-36_AG

    Plasmid

    #148839

    Purpose

    Mammalian Expression of DmBam_13-36

    Depositor

    Elisa Izaurralde

    Article

    Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

    Insert

    DmBam_13-36 (bam Fly)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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