We narrowed to 7,221 results for: ALP

1. EGFPC2-Gephyrin P1

   Plasmid

   #68815

   Purpose

   expression of fluorescent tagged Geph in mammalian cells

   Depositor

   Shiva Tyagarajan

   Article

   Tyagarajan et al J Biol Chem. 2013 Apr 5;288(14):9634-47.

   Insert

   Gephyrin (Gphn Rat)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   silent mutations on internal EcoRI site. Gephyri…

   Promoter

   CMV

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMT2-HA-RAPGEF2(S1244A/S1248A)

   Plasmid

   #110163

   Purpose

   Expression of human RAPGEF2 (S1244A/S1248A) in mammalian cells

   Depositor

   Daniele Guardavaccaro

   Article

   Magliozzi et al Dev Cell. 2013 Dec 9;27(5):574-85. doi:

   Insert

   RAPGEF2 (Rap guanine nucleotide exchange factor 2 (RAPGEF2 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   S1244A/S1248A

   Promoter

   CMV

   Available since

   June 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. FGG

   Plasmid

   #52031

   Purpose

   Expresses full-length Fibrinogen gamma chain precursor ectodomain in mammalian cells. Stop codon before C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   FGG (FGG Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Stop Codon at amino acid 454, before CD4, bio and…

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. FGB

   Plasmid

   #52030

   Purpose

   Expresses full-length Fibrinogen beta chain precursor ectodomain in mammalian cells. Stop codon before C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   FGB (FGB Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Stop Codon at amino acid 492, before CD4, bio and…

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMega-MaPylRS

   Plasmid

   #200225

   Purpose

   The plasmid encodes an orthogonal aminoacyl-tRNA synthetase/tRNA-CUA pair for amber codon suppression with Nε-Boc-lysine.

   Depositor

   Alanna Schepartz

   Article

   Fricke et al Nat Chem. 2023 Jun 1. doi: 10.1038/s4155

   Inserts

   Pyrrolysyl-tRNA synthetase
   pyrrolysyl-tRNA
   

   Use

   Tags

   none

   Expression

   Bacterial

   Mutation

   none

   Promoter

   proK-lacO and tacI

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. LRP8-bio-His

   Plasmid

   #51879

   Purpose

   Expresses full-length Low-density lipoprotein receptor-related protein 8 ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   LRP8 (LRP8 Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCR3-FLAG-Gephyrin P1

   Plasmid

   #68816

   Purpose

   Flag tagged Geph mammalian expression

   Depositor

   Shiva Tyagarajan

   Article

   Tyagarajan et al J Biol Chem. 2013 Apr 5;288(14):9634-47.

   Insert

   Gephyrin (Gphn Rat)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   silent mutations on internal EcoRI site. Gephyrin…

   Promoter

   CMV

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA3-beta-HA-BRCA2

   Plasmid

   #133553

   Purpose

   Mammalian expression vector with CMV promoter driving beta globin intron, HA tag, BRCA2 ORF

   Depositor

   David Livingston

   Article

   Chen et al Mol Cell. 1998 Sep;2(3):317-28.

   Insert

   BRCA2 (BRCA2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3-Flag-IDH1

   Plasmid

   #62906

   Purpose

   Expresses wildtype IDH1 in mammalian cells

   Depositor

   Yue Xiong

   Article

   Zhao et al Science. 2009 Apr 10;324(5924):261-5. do

   Insert

   isocitrate dehydrogenase 1 (NADP+), soluble (IDH1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pINDUCER20-α-Syn

    Plasmid

    #92200

    Purpose

    Retroviral overexpression vector (doxycycline-inducible) for α-Syn

    Depositor

    Huda Zoghbi

    Article

    Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

    Insert

    SNCA (SNCA Human)
    

    Use

    Lentiviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRSET iGluSnFR3 v857

    Plasmid

    #175186

    Purpose

    Fluorescent reporter for glutamate, third generation, variant 857 soluble form to express in bacteria.

    Depositor

    Kaspar Podgorski

    Article

    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

    Insert

    iGluSnFR3 v857
    

    Use

    Synthetic Biology

    Tags

    His tag, T7 leader, Xpress epitope

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pINDUCER-21-RUNX1

    Plasmid

    #97043

    Purpose

    Expresses RUNX1 in mammalian cells

    Depositor

    George Daley

    Article

    Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

    Insert

    Runt Related Transcription Factor 1 (RUNX1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tet on

    Available since

    Feb. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA3.1-IDH2-WT

    Plasmid

    #87926

    Purpose

    Expresses IDH2 (wild-type) in mammalian cells

    Depositor

    Reproducibility Project Cancer Biology

    Article

    Showalter et al Elife. 2017 Jun 27;6. pii: e26030. doi:

    Insert

    isocitrate dehydrogenase (NADP(+)) 2, mitochondrial (IDH2 Human)
    

    Use

    Tags

    none

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. DAG1-bio-His

    Plasmid

    #51651

    Purpose

    Expresses full-length Dystroglycan precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    DAG1 (DAG1 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3-Flag-IDH1-R132H

    Plasmid

    #62907

    Purpose

    Expresses R132 mutant IDH1 in mammalian cells

    Depositor

    Yue Xiong

    Article

    Zhao et al Science. 2009 Apr 10;324(5924):261-5. do

    Insert

    IDH1 (IDH1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Deleted Arg132 (R132)

    Promoter

    Available since

    April 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHAGE-PPP2R1A

    Plasmid

    #116777

    Purpose

    Lentiviral expression of PPP2R1A

    Depositor

    Kenneth Scott, Gordon Mills

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    PPP2R1A (PPP2R1A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. LTBP1-bio-His

    Plasmid

    #53421

    Purpose

    Expresses full-length Latent-transforming growth factor beta-binding protein 1 ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    LTBP1 (LTBP1 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. BSG-bio-His

    Plasmid

    #53146

    Purpose

    Expresses full-length Basigin ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    Basigin (BSG Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pAGT9569

    Plasmid

    #219431

    Purpose

    Transcriptional unit (MoClo-position 2): pAtRPS5a_PapE-4LF2-N-SpCas9i-N_tNOS

    Depositor

    Tom Schreiber

    Article

    Schreiber et al Mol Plant. 2024 Mar 22:S1674-2052(24)000

    Insert

    LB_AtRPS5a:PapE-4xLF2-NLS-SpCas9i-NLS:tNOS_RB (Position 2)
    

    Use

    CRISPR and Synthetic Biology; Moclo compatible le…

    Tags

    PapE-4LF2

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. nAChR beta2 YFP

    Plasmid

    #15107

    Depositor

    Henry Lester

    Article

    Nashmi et al J Neurosci. 2003 Dec 17. 23(37):11554-67

    Insert

    beta2-YFP-M (Chrnb2 Mouse)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 8, 2007

    Availability

    Academic Institutions and Nonprofits only

21. RUNX1_pcDNA6.2/EmGFP-Bsd

    Plasmid

    #176974

    Purpose

    Mammalian expression vector encoding RUNX1 and EmGFP-Bsd

    Depositor

    Roger Reeves

    Article

    Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

    Insert

    RUNX1 (RUNX1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. α‐eGFP

    Plasmid

    #160214

    Purpose

    Retroviral construct to express an eGFP-tagged version of the α subunit of AP2

    Depositor

    Sandra Schmid

    Article

    Mino et al Traffic. 2020 Sep;21(9):603-616. doi: 10

    Insert

    α‐eGFP (Ap2a2, Ap2a1 Mouse)
    

    Use

    Retroviral

    Tags

    eGFP at Hinge region

    Expression

    Mutation

    Brain-specific Insert

    Promoter

    Available since

    Dec. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. CMV-FLAG-TAOK2

    Plasmid

    #197862

    Purpose

    Expression of FLAG-tagged TAOK2 / PSK1 alpha in mammalian cells

    Depositor

    Jonathan Morris

    Article

    Koo et al Mol Cancer Ther. 2017 Nov;16(11):2410-24

    Insert

    TAOK2 (TAOK2 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Wild Type

    Promoter

    SV40

    Available since

    March 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. mouse a2b full length

    Plasmid

    #217812

    Purpose

    Full length mouse integrin a2b expression

    Depositor

    Timothy Springer

    Article

    Yuxin et al bioRxiv 2024.01.26.577394

    Insert

    ITGA2B (Itga2b Mouse)
    

    Use

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. tetO-AKT1

    Plasmid

    #170682

    Purpose

    doxycycline-inducible overexpression of AKT1

    Depositor

    Paul Burridge

    Article

    Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

    Insert

    AKT1 (AKT1 Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE promoter, Tet-ON

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pmCherryC1 VAP-A KD/MD

    Plasmid

    #226408

    Purpose

    Expression of human VAP-A (mutant K94D/M96D, unable to bind FFAT motifs) fused to mCherry in mammalian cells

    Depositor

    Catherine Tomasetto

    Article

    Di Mattia et al EMBO Rep. 2018 Jul;19(7). pii: embr.2017

    Insert

    VAP-A (VAPA Human)
    

    Use

    Tags

    T7-Xpress tag and mCherry

    Expression

    Mammalian

    Mutation

    K94D/M96D mutant, unable to bind FFAT motifs

    Promoter

    Available since

    Oct. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCEP4-HLA-c-myc-optiT1R3 ECD-MHC

    Plasmid

    #113961

    Purpose

    mammalian expression plasmid for c-myc-tagged human codon-optimized human T1R3 extracellular domain with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain fused to a canonical

    Depositor

    Erik Procko

    Article

    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

    Insert

    T1R3 (TAS1R3 Human)
    

    Use

    Tags

    Signal/leader sequence from HLA class I histocomp…

    Expression

    Mammalian

    Mutation

    residues 21-563 fused to a transmembrane tether

    Promoter

    cmv

    Available since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pRSET iGluSnFR3 v82

    Plasmid

    #175187

    Purpose

    Fluorescent reporter for glutamate, third generation, variant 82 soluble form to express in bacteria.

    Depositor

    Kaspar Podgorski

    Article

    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

    Insert

    iGluSnFR3 v82
    

    Use

    Synthetic Biology

    Tags

    His tag, T7 leader, Xpress epitope

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMega-MaFRSA

    Plasmid

    #200226

    Purpose

    The plasmid encodes an orthogonal aminoacyl-tRNA synthetase/tRNA-CUA pair for amber codon suppression with meta-trifluoromethylphenylalanine..

    Depositor

    Alanna Schepartz

    Article

    Fricke et al Nat Chem. 2023 Jun 1. doi: 10.1038/s4155

    Inserts

    Pyrrolysyl-tRNA synthetase
    pyrrolysyl-tRNA
    

    Use

    Tags

    none

    Expression

    Bacterial

    Mutation

    mutated Asparagine 166 to Alanine and Valine 168 …

    Promoter

    proK-lacO and tacI

    Available since

    June 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. EF1a_HNF1A_P2A_Hygro_Barcode

    Plasmid

    #120448

    Purpose

    Barcoded lentiviral vector to express HNF1A in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    HNF1A (HNF1A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. CD154-His-bio

    Plasmid

    #52337

    Purpose

    Expresses full-length Tumor necrosis factor ligand superfamily member 5 ectodomain in mammalian cells. N-terminal His tag, biotinylation sequence and rat Cd4d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    CD154 (CD40LG Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcrDNA3.1-PRKACA

    Plasmid

    #100890

    Purpose

    Mammalian expression of PRKACA

    Depositor

    Sanford Simon

    Article

    Honeyman et al Science. 2014 Feb 28;343(6174):1010-4. d

    Insert

    Protein Kinase CAMP-Activated Catalytic Subunit Alpha (PRKACA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Isoform 1 wild type

    Promoter

    CMV

    Available since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pQCXIP mScarlet-ER [TM(SAC1)]

    Plasmid

    #186572

    Purpose

    Expression of the Endoplasmic Reticulum marker mScarlet-ER [TM(SAC1)] in mammalian cells

    Depositor

    Catherine Tomasetto

    Article

    Zouiouich et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

    Insert

    SAC1 (SACM1L Human)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    C-terminal transmembrane region of the protein: 5…

    Promoter

    Available since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pLKO.1 shRUNX1 puro

    Plasmid

    #45816

    Depositor

    Kevin Janes

    Article

    Wang et al Proc Natl Acad Sci U S A. 2011 Oct 4;108

    Insert

    Runx1 (RUNX1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pLVX MLKL(wt)--2A-mCherry-puro

    Plasmid

    #231974

    Purpose

    Tet inducible expression of DmrB-Mlkl with mCherry to induce WT Mlkl expression

    Depositor

    Brian Ruffell

    Article

    Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

    Insert

    MLKL WT construct with bi-cistronic mCherry (Mlkl Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pQCXIP mScarlet-MOSPD2 RD/LD

    Plasmid

    #186476

    Purpose

    Expression of human MOSPD2 (mutant R404D/L406D, unable to bind FFAT motifs) fused to mScarlet in mammalian cells

    Depositor

    Catherine Tomasetto

    Article

    Zouiouich et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

    Insert

    MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
    

    Use

    Retroviral

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. sgTAZ-R-3

    Plasmid

    #229450

    Purpose

    knockout of WWTR1 (TAZ)

    Depositor

    Christopher Vakoc

    Article

    Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

    Insert

    sgTAZ-R-3 (WWTR1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6;bU6;EFS

    Available since

    Dec. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. EF1a_RUNX1_P2A_Hygro_Barcode

    Plasmid

    #120475

    Purpose

    Barcoded lentiviral vector to express RUNX1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    RUNX1 (RUNX1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pSIN4-EF1a-LMO2-IRES-Puro

    Plasmid

    #61064

    Purpose

    lentiviral vector for LMO2

    Depositor

    Igor Slukvin

    Article

    Elcheva et al Nat Commun. 2014 Jul 14;5:4372. doi: 10.

    Insert

    LMO2 (LMO2 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mutation

    Promoter

    EF1alpha

    Available since

    March 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAcBac1-Mb-Fluoro-Phe D6

    Plasmid

    #197577

    Purpose

    Expression of Methanosarcina barkeri (Mb) Fluoro-Phe D6 tRNA synthetase/Pyl-tRNA pair for the encoding of fluorinated phenylalanine derivatives at TAG codons in HEK293 cells

    Depositor

    Ryan Mehl

    Article

    Galles et al Nat Commun. 2023 Jan 4;14(1):59. doi: 10

    Inserts

    Mb Pyl Fluoro-Phe B5 synthetase
    Pyl-tRNA (4 copies)
    

    Use

    Tags

    Nuclear export sequence + FLAG

    Expression

    Mammalian

    Mutation

    N311A, C313M, V366G, W382T

    Promoter

    CMV and U6/H1

    Available since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. FLAG-MARK3-CR

    Plasmid

    #229422

    Purpose

    Lentiviral or overpexression of cDNA

    Depositor

    Christopher Vakoc

    Article

    Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

    Insert

    MARK3-CR (MARK3 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    silent mutation CRISPR resistant

    Promoter

    EFS

    Available since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. TGFB1-bio-His

    Plasmid

    #52185

    Purpose

    Expresses full-length Transforming growth factor beta-1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    TGFB1 (TGFB1 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. JAM3-bio-His

    Plasmid

    #51807

    Purpose

    Expresses full-length Junctional adhesion molecule C precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    JAM3 (JAM3 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. ADAM10-bio-His

    Plasmid

    #51641

    Purpose

    Expresses full-length ADAM10 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    ADAM10 (ADAM10 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. IL6ST-bio-His

    Plasmid

    #51806

    Purpose

    Expresses full-length Interleukin-6 receptor subunit beta precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    IL6ST (IL6R Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. M6PR-bio-His

    Plasmid

    #51924

    Purpose

    Expresses full-length Cation-dependent mannose-6-phosphate receptor precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    M6PR (M6PR Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pEXP5-aHL_spytag_his

    Plasmid

    #107334

    Purpose

    Monomeric SpyTag-6×-His alpha-hemolysin

    Depositor

    George Church

    Article

    Stranges et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

    Insert

    alpha-hemolysin-SpyTag-6xHis
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. TTR-bio-His

    Plasmid

    #52091

    Purpose

    Expresses full-length Transthyretin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    TTR (TTR Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pACEBac1-gamma-TuSC

    Plasmid

    #178079

    Purpose

    Insect cell expression of gamma-TuSC

    Depositor

    Tarun Kapoor

    Article

    Wieczorek et al J Cell Biol. 2021 Mar 1;220(3). pii: 211

    Inserts

    gamma-tubulin (TUBG1 Human)
    GCP2 (TUBGCP2 Human)
    GCP3 (TUBGCP3 Human)
    

    Use

    Tags

    TEV-His6

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pcDNA5-Flag-Med25

    Plasmid

    #64771

    Purpose

    Mammalian protein expression of flag-tagged Med25

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Sela et al J Biol Chem. 2012 Jun 29;287(27):23035-4

    Insert

    Med25 (MED25 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV

    Available since

    May 19, 2015

    Availability

    Academic Institutions and Nonprofits only