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Showing: 3801 - 3850 of 9181 results

  34. NeuA.pJL1

    Plasmid
    #199115
    Purpose
    In vitro expression of N-acylneuraminate cytodiylyltransfease (NeuA), which conjugates sialic acid and CTP to make activated sugar donor, CMP-sialic acid (see CSTI.PJL1 and PdST6.PJL1)
    Depositor
    Michael Jewett
    Article
    Thames et al ACS Synth Biol. 2023 Apr 11. doi: 10.102
    Insert
    N-acylneuraminate cytodiylyltransfease (NeuA)
    Use
    Tags
    Strep-tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    199115-plasmid-map-sequence-id-390797

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  36. pSLS.539

    Plasmid
    #184958
    Purpose
    Test effect of a1/a2 length on editing fabH using retron recombineering
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1 RT and recombineering ncRNA, fabH G954A, a1/a2 length: 12
    Use
    Tags
    Expression
    Bacterial
    Mutation
    fabH donor G954A
    Promoter
    T7/lac
    Available since
    Jan. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    184958-plasmid-map-sequence-id-381448

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  41. pSLS.543

    Plasmid
    #184960
    Purpose
    Test effect of a1/a2 length on editing murF using retron recombineering
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1 RT and recombineering ncRNA, murF G1359A, a1/a2 length: 12
    Use
    Tags
    Expression
    Bacterial
    Mutation
    murF donor G1359A
    Promoter
    T7/lac
    Available since
    Nov. 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    184960-plasmid-map-sequence-id-376545

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  42. pSLS.545

    Plasmid
    #184962
    Purpose
    Test effect of a1/a2 length on editing priB using retron recombineering
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1 RT and recombineering ncRNA, priB G1069A, a1/a2 length: 12
    Use
    Tags
    Expression
    Bacterial
    Mutation
    priB donor G1069A
    Promoter
    T7/lac
    Available since
    Nov. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    184962-plasmid-map-sequence-id-376122

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  43. pDR111_mannose_Bgal NLS

    Plasmid
    #188398
    Purpose
    Expresses beta-galactosidase by D-mannose promoter for Bacillus subtilis. There is a secretion peptide (PhoD) and nuclear localization signal (SV40) that is fused to beta-galactosidase.
    Depositor
    Christopher Contag
    Article
    Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:
    Insert
    lacZ
    Use
    Tags
    PhoD secretion peptide and SV40 NLS
    Expression
    Bacterial
    Mutation
    Promoter
    D-mannose
    Available since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    188398-plasmid-map-sequence-id-370473

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  44. pKP1693

    Plasmid
    #167595
    Purpose
    Express FepA with His6 tag between residues 393 and 394 of the mature amino acid sequence . Please note that mutation numbers are not based on start codon of insert.
    Depositor
    Kathleen Postle
    Article
    Gresock et al J Bacteriol. 2017 Apr 25;199(10). pii: J
    Insert
    FepA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    His6 tag inserted after residue 393 in the mature…
    Promoter
    Available since
    July 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    167595-plasmid-map-sequence-id-332904

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  45. pKB238

    Plasmid
    #170033
    Purpose
    Encodes LacI-controlled expression of surface-displayed NPY and PvirK-mNeonGreen reporter
    Depositor
    Jeffrey Tabor
    Article
    Brink et al bioRxiv
    Inserts
    mNeonGreen
    Neuropeptide Y (NPY Synthetic, Human, Escherichia coli)
    Use
    Synthetic Biology
    Tags
    Lpp, OmpA, and Tether
    Expression
    Bacterial
    Mutation
    Promoter
    Ptac and PvirK
    Available since
    July 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    170033-plasmid-map-sequence-id-336781

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  46. pKB235

    Plasmid
    #170024
    Purpose
    Encodes LacI-controlled expression of surface-displayed CCL20 and PvirK-mNeonGreen reporter
    Depositor
    Jeffrey Tabor
    Article
    Brink et al bioRxiv
    Inserts
    mNeonGreen
    CCL20 (CCL20 Synthetic, Human, Escherichia coli)
    Use
    Synthetic Biology
    Tags
    Lpp, OmpA, and Tether
    Expression
    Bacterial
    Mutation
    Promoter
    Ptac and PvirK
    Available since
    July 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    170024-plasmid-map-sequence-id-336757

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  47. pMRBad-C-wtBlc

    Plasmid
    #168257
    Purpose
    C-fragment. This plasmid can be co-transformed with either Plasmid 168259: pET11a-N-wtBlc or Plasmid 168472: pET11a-N-DiB2 to obtain wtBlc‐split or DiB2‐split protein, correspondingly
    Depositor
    Jens Meiler
    Article
    Bozhanova et al FEBS Lett. 2021 Jan;595(2):206-219. doi:
    Insert
    C-fragment of wtBlc
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    araBAD
    Available since
    May 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    168257-plasmid-map-sequence-id-332728

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Showing: 3801 - 3850 of 9181 results