19,178 results

1. pBS2E

   Plasmid

   #55169

   Purpose

   Empty vector, integration at lacA, ampr, mlsr

   Depositor

   Thorsten Mascher

   Article

   Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

   Type

   Empty backbone

   Use

   Synthetic Biology; Bacillus biobrick box

   Tags

   Expression

   Mutation

   Promoter

   none

   Available since

   July 22, 2014

   Availability

   Academic Institutions and Nonprofits only

2. mCherry-Peroxisomes-2

   Plasmid

   #54520

   Purpose

   Localization: Peroxisomes, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Type

   Empty backbone

   Use

   Tags

   Peroximal Targeting Signal 1 (PTS1) and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hSynapsin1-axon-jGCaMP8m-P2A-mRuby3

   Plasmid

   #172922

   Purpose

   For enriched expression of jGCaMP8m in axons with cytosolic expression of mRuby3

   Depositor

   GENIE Project

   Has Service

   AAV1 and AAV9

   Insert

   axon-jGCaMP8m-P2A-mRuby3
   

   Use

   AAV

   Tags

   GAP43 palmitoylation domain (N terminal on insert…

   Expression

   Mammalian

   Mutation

   Promoter

   hSynapsin1

   Available since

   Aug. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSL1425 (pSPIN, pCDF backbone)

   Plasmid

   #160735

   Purpose

   Single-plasmid V. cholerae CAST system, encodes all proteins, crRNA, and donor DNA. Entry vector encodes non-targeting crRNA and has BsaI sites for spacer cloning. pCDF backbone.

   Depositor

   Samuel H. Sternberg

   Article

   Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

   Insert

   VchCAST proteins, crRNA, and donor DNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119

   Available since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-hNurr1-NE

   Plasmid

   #102363

   Purpose

   Overexpression of NR4A2 nuclear receptor subfamily 4 group A member 2 in mammalian cells

   Depositor

   Shu Leong Ho

   Insert

   NR4A2 nuclear receptor subfamily 4 group A member 2 (NR4A2 Human)
   

   Use

   Tags

   NE

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti CMV Blast DEST (706-1)

   Plasmid

   #17451

   Purpose

   3rd gen lentiviral Gateway destination vector, expression, CMV promoter, Blast 

   Depositor

   Eric Campeau, Paul Kaufman

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral; Destination vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. ER-GCaMP6-150

   Plasmid

   #86918

   Purpose

   Fluorescent reporter for ER calcium signaling

   Depositor

   Timothy Ryan

   Article

   de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

   Insert

   ER-GCaMP6-150
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   K78H, T302L, R303P, A317E, D324G, D360G, D380Y, T…

   Promoter

   CAMKII

   Available since

   March 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. shYAP1 # 1

   Plasmid

   #42540

   Depositor

   William Hahn

   Article

   Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

   Insert

   YAP1 (YAP1 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. GFP-P4M-SidMx2

   Plasmid

   #51472

   Purpose

   Biosensor for PtdIns4P

   Depositor

   Tamas Balla

   Article

   Hammond et al J Cell Biol. 2014 Apr 7.

   Inserts

   P4M-SidM(546-647)
   P4M-SidM(546-647)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. tdTomato-H2B-6

    Plasmid

    #58101

    Purpose

    Localization: Nucleus/Histones, Excitation: 554, Emission: 581

    Depositor

    Michael Davidson

    Article

    Planchon et al Nat Methods. 2011 May;8(5):417-23. doi:

    Insert

    H2B (H2BC11 Human)
    

    Use

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    D26G and V119I in H2B

    Promoter

    CMV

    Available since

    Dec. 23, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGTMV

    Plasmid

    #118755

    Purpose

    Binary plasmid with a tobacco mosaic virus infectious clone; Agrobacterium-mediated transformation required for expression in plants.

    Depositor

    Jose-Antonio Daros

    Article

    Shi et al Plant Biotechnol J. 2018 Nov 22. doi: 10

    Insert

    Tobacco mosaic virus
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Cauliflower mosaic virus 35S

    Available since

    Nov. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-Flag-NSF

    Plasmid

    #74924

    Purpose

    Mammalian expression of human NSF full-length (744 aa)

    Depositor

    Elisa Greggio

    Article

    Belluzzi et al Mol Neurodegener. 2016 Jan 13;11(1):1. d

    Insert

    NSF (NSF Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1_3xFlagEzh2 WT

    Plasmid

    #173717

    Purpose

    WT EZH2 expression plasmid with 3XFLAG tag

    Depositor

    Thomas Cech

    Article

    Long et al Nat Genet. 2020 Sep;52(9):931-938. doi:

    Insert

    EZH2 (EZH2 Human)
    

    Use

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. c-myc-PT3EF1a

    Plasmid

    #92046

    Purpose

    Expresses c-Myc in mammalian cells

    Depositor

    Xin Chen

    Article

    Liu et al Hepatology. 2017 Mar 30. doi: 10.1002/he

    Insert

    C-MYC (MYC Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    July 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. dCas-hHDAC3-R265P

    Plasmid

    #103786

    Purpose

    Expresses dCas9 fused to human HDAC3 with R265P mutation

    Depositor

    Zhaolan Zhou

    Article

    Kwon et al Nat Commun. 2017 May 12;8:15315. doi: 10

    Insert

    dCas9-HDAC3-R265P (HDAC3 Human, Synthetic, S. pyogenes)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Changed arginine 265 to proline

    Promoter

    EF1A

    Available since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. PX551

    Plasmid

    #60957

    Purpose

    pAAV-pMecp2-SpCas9-spA (AAV-SpCas9). AAV plasmid expressing Cas9 in neurons under control of truncated mecp2 promoter.

    Depositor

    Feng Zhang

    Article

    Swiech et al Nat Biotechnol. 2014 Oct 19. doi: 10.103

    Insert

    SpCas9
    

    Use

    AAV and CRISPR

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    pMecp2

    Available since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDH-EF1-Large BiT - SNAP-tag - Beta-actin

    Plasmid

    #171019

    Purpose

    Lentiviral expression of a Large BiT-SNAP-tag-Beta-actin fusion in mammalian cells

    Depositor

    Angus Johnston

    Article

    Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

    Insert

    LargeBit-SNAPtag- Beta-actin
    

    Use

    Lentiviral and Luciferase

    Tags

    Beta actin and LargeBiT

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. 3xFLAG-dCas9/pCMV-7.1

    Plasmid

    #47948

    Purpose

    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

    Depositor

    Hodaka Fujii

    Article

    Fujita et al Biochem Biophys Res Commun. 2013 Sep 13;

    Insert

    3xFLAG-dCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    human codon-optimized, D10A + H840A (catalyticall…

    Promoter

    CMV

    Available since

    Sept. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-CAG-FLEXFRT-ChR2(H134R)-mCherry

    Plasmid

    #75470

    Purpose

    Flp-dependent Chr2-mCherry encoded in an AAV vector for rAAV production and expression in mammalian cells

    Depositor

    Connie Cepko

    Article

    Tang et al Elife. 2016 May 20;5. pii: e15312. doi:

    Has Service

    AAV1

    Insert

    Reversed ChR2(H134R)-mCherry
    

    Use

    AAV and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-ATR Kinase dead

    Plasmid

    #31612

    Depositor

    Aziz Sancar

    Article

    Jiang et al Mol Cell Biol. 2006 Jan . 26(1):39-49.

    Insert

    ATR (ATR Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    K2327R (kinase dead)

    Promoter

    Available since

    Nov. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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21. CMV-FlicR1

    Plasmid

    #74142

    Purpose

    Mammalian expression of FlicR1: A bright red fluorescent genetically encoded voltage indicator

    Depositor

    Robert Campbell

    Article

    Abdelfattah et al J Neurosci. 2016 Feb 24;36(8):2458-72. d

    Insert

    FlicR1
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pSFFV_mNG2(11)1-10

    Plasmid

    #82610

    Purpose

    Lentiviral vector of constitutive expression of mNeonGreen2(1-10)

    Depositor

    Bo Huang

    Article

    Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

    Insert

    mNeonGreen2(1-10)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    K128M, S142T, R150M, G172V and K213M

    Promoter

    SFFV

    Available since

    May 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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23. SpCas9 nickase for Split-PE (pJUL2497)

    Plasmid

    #190104

    Purpose

    Expresses nickase SpCas9(H840A) for Split-PE use

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

    Insert

    nSpCas9
    

    Use

    Tags

    XTEN-bpNLS and bpNLS

    Expression

    Mammalian

    Mutation

    H840A

    Promoter

    CMV

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. SPLICS Po-Mt Short P2A

    Plasmid

    #164116

    Purpose

    Detect the short Peroxisomes-mitochondria contact

    Depositor

    Tito Calì, Marisa Brini

    Article

    Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

    Insert

    Split GFP Po-Mt Short P2A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. FTL

    Plasmid

    #100750

    Purpose

    Part of FerriTag expression system. Untagged Ferritin light chain in mammalian expression vector.

    Depositor

    Stephen Royle

    Article

    Clarke et al Nat Commun. 2018 Jul 4;9(1):2604. doi: 1

    Insert

    FTL (FTL Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMJ920

    Plasmid

    #42234

    Depositor

    Jennifer Doudna

    Article

    Jinek et al elife. 2013;2:e00471. doi: 10.7554/eLife

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    GFP, HA epitope, and NLS (nuclear localization si…

    Expression

    Mammalian

    Mutation

    codon-optimized synthetic DNA sequence

    Promoter

    CMV

    Available since

    March 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pSicoR human Dicer1

    Plasmid

    #14763

    Depositor

    Tyler Jacks

    Article

    Kumar et al Nat Genet. 2007 Apr 1. ():.

    Insert

    Dicer shRNA (DICER1 Human)
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 5, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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28. pLPT119

    Plasmid

    #85526

    Purpose

    Repressilator without degradation tags, with integrated reporter

    Depositor

    Johan Paulsson

    Article

    Potvin-Trottier et al Nature. 2016 Oct 12;538(7626):514-517. d

    Inserts

    mCfp
    mVenus
    tetR
    lacI
    cI
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pDSG358

    Plasmid

    #115599

    Purpose

    surface-displayed Ag1; medium copy

    Depositor

    Ingmar Riedel-Kruse

    Article

    Glass et al Cell. 2018 Jul 26;174(3):649-658.e16. do

    Insert

    regulated adhesin construct
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    See depositor comments below

    Promoter

    Available since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pTRIP-SFFV-mtagBFP-2A RIGI WT

    Plasmid

    #167289

    Purpose

    Lentiviral expression construct encoding a TagBFP in frame with a self-cleaving wild-type RLR sensor RIG-I

    Depositor

    Agnel Sfeir

    Article

    Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

    Insert

    DDX58 (RIGI Human)
    

    Use

    Lentiviral

    Tags

    tagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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