We narrowed to 59,827 results for: Gene
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Plasmid#90095PurposeBacterial expression plasmid for protein purificationDepositorInserthuAsCpf1
Tags3xHA, 6xHis, MBP, Nucleoplasmin NLS, and TEV site…ExpressionBacterialAvailable SinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAV-EFS-NLS-CasRx-U6-DR-sgRNA_LacZ-DR
Plasmid#154003PurposeVector expressed CasRx and sgRNA_LACZ for AAV packageDepositorInsertCasRx
UseAAV and CRISPRPromoterEFSAvailable SinceJuly 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJMP1
Plasmid#79873PurposeBacillus subtilis dCas9 expression vector; integrates into lacA/ganADepositorInsertdCas9
UseCRISPRExpressionBacterialMutationD10A, H840APromoterxylAAvailable SinceOct. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSW071
Plasmid#140037PurposePCC 11901 fadD locus KO with a kanamycin resistance cassetteDepositorInsertKanamycin resistance gene
UseGene knockout in cyanobacteriaPromoterKanRAvailable SinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGen2.1-redCALWY-1
Plasmid#50739PurposeExpresses redCALWY-1 in mammalian cells. FRET sensor for zinc using mOrange2-R125I and mCherry-R125I as donor and acceptor domains. Allows multiparameter imaging together with CFP-YFP FRET sensors.DepositorInsertgene for redCALWY-1 Zn2+ sensor
ExpressionMammalianAvailable SinceJan. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
TGFB1_pLX307
Plasmid#98377PurposeLentiviral expression of TGFB1DepositorAvailable SinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pExodus CMV.Trex2
Plasmid#40210PurposeMammalian expression of codon-optimized Trex2.DepositorInsertThree prime repair exonuclease 2 (Trex2 Mouse)
ExpressionBacterial and MammalianPromoterCMV and T7Available SinceSept. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
evoBxb1
Plasmid#222338PurposePlasmid for expressing evolved Bxb1 in the mammalian cellsDepositorInsertCodon-optimized evolved Bxb1
ExpressionMammalianPromoterCMVAvailable SinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CBh-mKate2-IRES-MCS (WT-WT)
Plasmid#105921PurposeAAV-mediated gene expression from CBh promoter. Contains canonical left and right AAV2 ITRsDepositorHas ServiceAAV2InsertsmKate2
IRES-Multiple cloning site
UseAAVMutationcodon optimizedPromoterCBh and same CBh promoter as mKate2Available SinceFeb. 15, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
AAVS1 pegRNA1 with trimmed attP
Plasmid#222346PurposepegRNA 1 plasmid used for PASSIGE-mediated Bxb1 attP installationDepositorInsertAAVS1 attP-installation pegRNA1
UseCRISPRAvailable SinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSAM_AraC
Plasmid#91569PurposeMariner transposon mutagenesis & sequencing vector - Transposon contains KanR and Illumina sequencing adapters. Arabinose promoter expresses transposase. Ampicillin resistance on backbone.DepositorInsertAraC/PBAD
ExpressionBacterialAvailable SinceAug. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBBR_Cas9_sp1
Plasmid#154851PurposeExpresses SpCas9 and sgRNA that targets the upp gene in Rhodobacter sphaeroides genomeDepositorInsertsSpcas9 gene
sgRNA scaffold
UseCRISPRTags6xHisMutationCodon harmonized variant for expression in Rhodob…Available SinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAcrF2
Plasmid#89234PurposePlasmid contains the gene for anti-CRISPR protein AcrF2 (gene 30 from bacteriophage D3112), with N-terminal 6his tag and TEV protease cleavage siteDepositorInsertAcrF2
Tags6xHis-TEVAvailable SinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAV-CMVc-Cas9
Plasmid#106431PurposeExpresses SpCas9 from the CMVc promoter in AAV backboneDepositorInsertCas9
UseAAV and CRISPRTagsHA-NLS and NLSExpressionMammalianPromoterCMVcAvailable SinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CAG-empty-ires-EGFP
Plasmid#122953PurposeLentiviral expression empty vector under CAG promoter with EGFP expressionDepositorTypeEmpty backboneUseLentiviralAvailable SinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn1-mNeonGreen
Plasmid#99135PurposeAn AAV genome that ubiquitously expresses the fluorescent protein mNeonGreen from the hSyn1 promoterDepositorInsertmNeonGreen
UseAAVExpressionMammalianPromoterhSyn1Available SinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pClneoMyc-LATS1
Plasmid#66851PurposeExpress Myc-tagged LATS1 in mammalian cellsDepositorInsertLATS1 (LATS1 Human)
TagsMycExpressionMammalianMutationP44L mutation in LATS1 compared to GenBank refere…PromoterCMVAvailable SinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide_DKO
Plasmid#183193PurposePlasmid with two U6 promoters and two gRNA scaffolds that allows for inserting two gRNAs for combinatorial CRISPR Screen or double-knock-out (DKO) screenDepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianPromoterhU6, mU6Available SinceAug. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
lenti EF1a-FLAG-dCas9-VPR
Plasmid#114195PurposeLentivirus compatible dCas9-VPR construct driven by the EF1a promoterDepositorInsertdCas9-VPR
UseLentiviralTagsFLAGExpressionMammalianPromoterEF1aAvailable SinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
MYC_pLX307
Plasmid#98363PurposeLentiviral expression of MYCDepositorAvailable SinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pU6-AAVS1-5-1kbdonor
Plasmid#234735PurposePseCAST crRNA targeting AAVS1-5, with 1 kb donor transposonDepositorInsertAAVS1-5 crRNA
ExpressionMammalianAvailable SinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
SMARCA2-GFP
Plasmid#65390PurposeGFP-tagged BRD protein, which can be used to be expressed in mammalian cells.DepositorAvailable SinceOct. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAVS1 pegRNA2 with trimmed attP
Plasmid#222347PurposepegRNA 2 plasmid used for PASSIGE-mediated Bxb1 attP installationDepositorInsertAAVS1 attP-installation pegRNA2
UseCRISPRAvailable SinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-U6-dgRNA-CAG-MPH
Plasmid#106259PurposeExpresses MPH co-activator from the CAG promoter and one U6-driven dgRNA in AAV backboneDepositorAvailable SinceMay 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
L4440_BioBrick-sgRNA
Plasmid#177783PurposeOverexpression of sgRNAs in E. coli HT115, sgRNA overexpression scaffold; for ingestion by C. elegansDepositorTypeEmpty backboneExpressionBacterialAvailable SinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMGS54 (AID-P2A-Hygromycin)
Plasmid#126583PurposeAn AID-P2A-HygR cassette to tag the protein of interests with auxin-inducible degron at the c-terminalDepositorInsertAID-P2A-HygR
UseOtherAvailable SinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
ppBAalbNixE1E2E3E4 PUb-DsRed
Plasmid#173665Purposetransgenesis vector for Aedes albopictus ectopic expression of the Nix geneDepositorInsertsNix masculinisation gene
DsRed
ExpressionInsectMutationNix isoforms with 4 exonsAvailable SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-10:LMNB1-mEGFP
Plasmid#87422PurposeHomology arms and linker-mEGFP sequence for N-terminus tagging of human LMNB1DepositorInsertLMNB1 Homology Arms with mEGFP-linker (LMNB1 Human)
UseCRISPR; Donor templateTagsmEGFP-linkerExpressionMammalianMutationhomology arms contain point mutations to disrupt …Available SinceMarch 15, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
AAV-U6gRNA1-U6gRNA2-TnT-Cre
Plasmid#87682PurposeAAV vector for U6 driven expression of two gRNAs, and cardiomyocyte specific expression of Cre recombinase.DepositorInsertsgRNA1
gRNA2
Cre
UseAAVPromoterU6 and cTnTAvailable SinceMarch 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMVMagFRET-6-NLS
Plasmid#50757PurposeExpresses MagFRET-6 in the nucleus of mammalian cells. Weak affinity control FRET sensor for MagFRET-1DepositorInsertgene encoding for MagFRET-6 with NLS
ExpressionMammalianAvailable SinceJan. 16, 2014AvailabilityAcademic Institutions and Nonprofits only