165,951 results

1. pcDNA TrkB

   Plasmid

   #24088

   Depositor

   Moses Chao

   Article

   Yano et al J Neurosci. 2001 Feb 1. 21(3):RC125.

   Insert

   TrkB (Ntrk2 Rat)
   

   Expression

   Mammalian

   Mutation

   R133I

   Available Since

   Jan. 2, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-2dV-Camui (T305D/T306D)

   Plasmid

   #220368

   Purpose

   T305D/T306D phosphomimetic mutant of pCAG-2dV-Camui

   Depositor

   Ryohei Yasuda

   Article

   Jain et al Nature. 2024 Oct 9. doi: 10.1038/s41586-

   Insert

   mVenus(Y145W)-mVenus(Y145W)-rat CaMK2 alpha(T305D/T306D)-mEGFP(A206K) (Camk2a Rat, Synthetic)
   

   Tags

   mEGFP(A206K) and mVenus(Y145W)-mVenus(Y145W)

   Expression

   Mammalian

   Mutation

   changed both Threonine 305 and 306 to aspartic ac…

   Promoter

   CAG

   Available Since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGLTR-X-GFP

   Plasmid

   #58245

   Purpose

   GATEWAY-compatible lentiviral conditional RNAi delivery vector; SFFV-driven expression of TetR and eGFP

   Depositor

   Stephan Geley

   Article

   Sigl et al PLoS One. 2014 May 19;9(5):e97764. doi:

   Inserts

   attR1-Cm(R)-CcdB-attR2
   TetR-NLS-T2A-GFP
   

   Use

   Lentiviral and RNAi; Gateway destionation vector

   Tags

   GFP, NLS, T2A, and TetR

   Expression

   Mammalian

   Promoter

   SFFV and no

   Available Since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJEP11-AAV-U6/TO-sgRNA(Tet2)-CMV-TetR-P2A-eGFP-KASH-pA

   Plasmid

   #82705

   Purpose

   AAV backbone with a full length U6/TO promoter driving expression of a sgRNA that targets exon 3 of the mouse Tet2 locus. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.

   Depositor

   Jonathan Ploski

   Article

   de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do

   Insert

   U6/TO gRNA Expression Cassette Containing Tet2 gRNA
   

   Use

   AAV and CRISPR

   Tags

   CMV promoter driving expression of TetR-P2A-GFP-K…

   Expression

   Mammalian

   Available Since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. PB09-TRE-beta-globin-miR-124-EF1a-GFP

   Plasmid

   #117318

   Purpose

   Overexpression of miR-124 in eurkaryotic cells, encoded in a human beta-globin intron

   Depositor

   Volker Busskamp

   Article

   Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

   Insert

   hsa-miR-124-1 (MIR124-1 Human)
   

   Use

   Transposon-mediated integration

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   Tet-inducible promoter

   Available Since

   Jan. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. p413TEF-PKM1

   Plasmid

   #34607

   Depositor

   Markus Ralser

   Article

   Bluemlein et al Oncotarget. 2011 May;2(5):393-400.

   Insert

   PKM1 (PKM Human)
   

   Expression

   Yeast

   Promoter

   TEF

   Available Since

   Feb. 16, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pIG-703pUC19-tNGFR-P2A-BACH2

   Plasmid

   #186112

   Purpose

   Knockin of truncated NGFR to target gene

   Depositor

   Alexander Marson

   Article

   Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

   Insert

   BACH2 (BACH2 Human)
   

   Use

   CRISPR

   Mutation

   WT with tNGFR sequence

   Available Since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-2dV-Camui (T286A)

   Plasmid

   #220367

   Purpose

   T286A phosphodead mutant of pCAG-2dV-Camui

   Depositor

   Ryohei Yasuda

   Article

   Jain et al Nature. 2024 Oct 9. doi: 10.1038/s41586-

   Insert

   mVenus(Y145W)-mVenus(Y145W)-rat CaMK2 alpha(T286A)-mEGFP(A206K) (Camk2a Rat, Synthetic)
   

   Tags

   mEGFP(A206K) and mVenus(Y145W)-mVenus(Y145W)

   Expression

   Mammalian

   Mutation

   changed Threonine 286 to Alanine

   Promoter

   CAG

   Available Since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR221_SLC4A5

   Plasmid

   #132284

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC4A5 (SLC4A5 Human)
   

   Expression

   Mammalian

   Available Since

   Nov. 14, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pTR-469 JUNB-tNGFR

    Plasmid

    #186060

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    JUNB (JUNB Human)
    

    Use

    CRISPR

    Mutation

    WT with tNGFR sequence

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTR-472 FOXO1-tNGFR

    Plasmid

    #186062

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    FOXO1 (FOXO1 Human)
    

    Use

    CRISPR

    Mutation

    WT with tNGFR sequence

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pafG-CBE-3T

    Plasmid

    #220374

    Purpose

    for hA3A-nCas9 and gRNA expression in Aspergillus flavus

    Depositor

    Xue Gao

    Article

    Zhao et al J Am Chem Soc. 2023 Jan 11;145(1):413-42

    Inserts

    hA3A-nCas9
    tRNA and gRNA scaffold
    

    Use

    CRISPR

    Promoter

    Ptef1 and tRNA

    Available Since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. tetO-ETS2

    Plasmid

    #170685

    Purpose

    doxycycline-inducible overexpression of ETS2

    Depositor

    Paul Burridge

    Article

    Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

    Insert

    ETS2 (ETS2 Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Expression

    Mammalian

    Promoter

    TRE promoter, Tet-ON

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1_hSMAD4

    Plasmid

    #212710

    Purpose

    The ORF of human SMAD4, variant 1 cDNA amplified from the RT human liver RNA was cloned at the NheI/HindIII sites of the pcDNA3.1(+), the mammalian expression vector.

    Depositor

    Catharine Ross, Reza Zolfaghari

    Insert

    Homo sapiens SMAD4 (SMAD4 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEY17

    Plasmid

    #191023

    Purpose

    flp-18 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

    Depositor

    Oliver Hobert, Eviatar Yemini

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    flp-18 CELE_Y48D7A.2 (flp-18 Nematode)
    

    Expression

    Worm

    Mutation

    None

    Available Since

    Nov. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. GFP-LacI-polylinker

    Plasmid

    #179271

    Purpose

    Cloning of GFP-LacI fusion proteins for LacI/LacO tethering

    Depositor

    Lienhard Schmitz

    Article

    Haas et al Front Cell Dev Biol. 2020 Sep 1;8:852. d

    Type

    Empty backbone

    Tags

    GFP and LacI

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPAP002

    Plasmid

    #153489

    Purpose

    Level 1 episomal expression plasmid for P. pastoris

    Depositor

    Sylvestre Marillonnet

    Article

    Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTR-471 IRF4-tNGFR

    Plasmid

    #186061

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    IRF4 (IRF4 Human)
    

    Use

    CRISPR

    Mutation

    WT with tNGFR sequence

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. PB09-TRE-beta-globin-miR-ctrl-EF1a-GFP

    Plasmid

    #117319

    Purpose

    Overexpression of miR-124-surrounding locus (not miR-124) in eurkaryotic cells, encoded in a human beta-globin intron

    Depositor

    Volker Busskamp

    Article

    Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

    Insert

    hsa-miR-CTRL
    

    Use

    Transposon-mediated integration

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    miR-124 deleted

    Promoter

    Tet-inducible promoter

    Available Since

    Jan. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMT643

    Plasmid

    #8610

    Depositor

    Robert Hartley

    Article

    Hartley et al Biochemistry 1993 Jun 15;32(23):5978-84.

    Insert

    barstar A
    

    Expression

    Bacterial

    Mutation

    Two cysteines of barstar changed to alanine, C40A…

    Available Since

    Jan. 17, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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