We narrowed to 11,716 results for: VARS

Showing: 3161 - 3200 of 11716 results

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pSb_init containing anti SARS-CoV-2_RBD sybody Sb#14

Plasmid

#153522

Purpose

Expression of the anti SARS-CoV-2_RBD sybody Sb#14

Depositor

Markus Seeger

Article

Walter et al bioRxiv 2020.04.16.045419

Insert

Sb#14

Expression

Bacterial

Promoter

pBAD

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pCFJ1149 - intron 3 smu-2 PATCs

Plasmid

#161516

Purpose

PATC-rich intron number three from smu-2 to stimulate germline expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

Intron 3 from smu-2

Expression

Worm

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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p426 72Q GPD

Plasmid

#1183

Depositor

Susan Lindquist

Article

Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

Insert

htt 72Q (HTT Human)

Tags

GFP

Expression

Yeast

Available Since

May 25, 2005

Availability

Academic Institutions and Nonprofits only
pCFJ2359 - intron GG2 - 250bp

Plasmid

#159878

Purpose

Golden Gate compatible intron with PATCs for reduced germline silencing

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

intron GG2 - 250bp

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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mTFP1-C-Src-7

Plasmid

#55472

Purpose

Localization: Membrane/Vesicles, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

C-Src

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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PKAcs-GGS4X-GFP

Plasmid

#108583

Purpose

Expresses PKAcs-GFP fusion in mammalian cells with flexible linker

Depositor

Robert Hughes

Article

Norris et al FEBS Open Bio. 2018 Apr 25;8(6):1029-104

Insert

protein kinase A, catalytic subunit

Tags

GFP

Expression

Mammalian

Mutation

H87Q and W196R

Available Since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pICH47732:PtFCP:ShBle

Plasmid

#104893

Purpose

Expresses ShBle with FCP promoter/terminator, Golden Gate Assembly, for Phaeodactylum transformation.

Depositor

Thomas Mock

Article

Graff van Creveld et al Front Microbiol. 2021 Sep 8;12:688199. d

Insert

ShBle

Use

Phaeodactylum; golden gate assembly

Tags

PtFCP promoter and terminator

Available Since

Dec. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pHL 1903

Plasmid

#53020

Purpose

AmpR + fimS::gfpAAV::Asp terminator + CamR + ColE1

Depositor

Han Lim

Article

Hung et al Nat Commun. 2014 Aug 4;5:4574. doi: 10.1

Insert

GFP

Use

Synthetic Biology

Tags

AAV, Asp terminator, and fimS

Expression

Bacterial

Promoter

fimS

Available Since

May 14, 2014

Availability

Academic Institutions and Nonprofits only
G1052C_BattB(GA)_EGFP_IRES-Spycatcher-sfmCherry3C(1-10)-P2A-shBleR

Plasmid

#200636

Purpose

Strong EGFP fluorescence recombination plasmid for Matreyek Bxb1(GA) landing pad in dual landing pad cells

Depositor

Kenneth Matreyek

Article

Roelle et al ACS Synth Biol. 2023 Nov 3. doi: 10.1021

Insert

EGFP

Expression

Mammalian

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pMS15: pTwinStrep-SUMO-AvTnsB

Plasmid

#168148

Purpose

AvTnsB expression construct for protein purification.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Insert

AvCAST TnsB

Tags

6X His, Twin Strep and SUMO

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pMS16: pTwinStrep-SUMO-AvTnsC

Plasmid

#168149

Purpose

AvTnsC expression construct for protein purification.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Insert

AvCAST TnsC

Tags

6X His, Twin Strep and SUMO

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pMS14: pTwinStrep-SUMO-AvTnsA

Plasmid

#168147

Purpose

AvTnsA expression construct for protein purification.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Insert

AvCAST TnsA

Tags

6X His, Twin Strep and SUMO

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pMS8: pTns(AvCAST)

Plasmid

#168141

Purpose

Inducible expression of AvCAST TnsA, TnsB, TnsC, TniQ and TnsD proteins.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Inserts

AvCAST Tns proteins (TnsA, TnsB, TnsC and TniQ)
AvTnsD

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pAB061i2

Plasmid

#79229

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

Ppro1 rpoZ-HA4 (Y87A); PlacIq lacI

Expression

Bacterial

Promoter

Ppro1; PlacIq

Available Since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

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CMV plasmid

Plasmid

#169739

Purpose

Unregulated "open-loop" CMV promoter plasmid. This plasmid encodes eGFP to report circuit output levels.

Depositor

Francois St-Pierre

Article

Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

Insert

eGFP

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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pML107-kanR468

Plasmid

#162041

Purpose

Expresses Cas9 and contains kanR468 guide which targets the kanR gene

Depositor

Jolanda Van Leeuwen

Article

Parts et al Mol Syst Biol. 2021 May;17(5):e10138. do

Insert

kanR468 guide

Use

CRISPR

Expression

Yeast

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pV1539

Plasmid

#111434

Purpose

Genedrive entry vector for Candida albicans

Depositor

Gerald Fink

Article

Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

Insert

CaCas9/sgRNA-BsmBI stuffer

Use

CRISPR

Expression

Yeast

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pZH520

Plasmid

#102673

Purpose

Tetracycline inducible expression of GFPmut2 with constitutive TetR; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

Depositor

Zach Hensel

Article

Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

Insert

GFPmut2

Expression

Bacterial

Available Since

Dec. 5, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCFJ2370 - intron GG3 - 250bp

Plasmid

#159879

Purpose

Golden Gate compatible intron with PATCs for reduced germline silencing

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

intron GG3 - 250bp

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
GluR2_adRNA(1,20,6)-ADAR2

Plasmid

#124620

Purpose

Expresses adRNA (1,20,6) targeting the RAB7A transcript along with the ADAR2

Depositor

Prashant Mali

Article

Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

Insert

ADAR2

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pMP4516

Plasmid

#107775

Purpose

Expression of ECFP

Depositor

Herman Spaink

Article

Stuurman et al Mol Plant Microbe Interact. 2000 Nov;13(

Insert

pLacZ-ECFP in pBBR1-mcs5

Tags

pLacZ-ECFP

Expression

Bacterial

Promoter

pLacZ

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pAB082b

Plasmid

#79234

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

Ppro1 rpoZ-Cry1Ac-d3

Expression

Bacterial

Promoter

Ppro1

Available Since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pZH521

Plasmid

#102674

Purpose

Tetracycline inducible expression of GFPmut2 with constitutive TetR; weak ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

Depositor

Zach Hensel

Article

Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

Insert

GFPmut2

Expression

Bacterial

Available Since

Dec. 5, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
MECP2 - R133C in pENTR

Plasmid

#216594

Purpose

gateway cloning donor vector containing MECP2 ASD mutation

Depositor

Douglas Allan, Christopher Loewen

Insert

MECP2 (MECP2 Human)

Use

Gateway entry plasmid

Mutation

R133C

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2+ Bmi1-cherry-HA

Plasmid

#30516

Depositor

John Gurdon

Article

Pasque et al EMBO J. 2011 May 6;30(12):2373-87. doi:

Insert

Bmi1 (Bmi1 Mouse)

Use

Xenopus/zebrafish expression

Tags

cherry and triple HA

Expression

Mammalian

Mutation

NO STOP

Available Since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pAcGFP1-N1-RvLEAM

Plasmid

#90035

Purpose

To express GFP-tagged RvLEAM in mammalian cells transiently

Depositor

Takekazu Kunieda

Article

Tanaka et al PLoS One. 2015 Feb 12;10(2):e0118272. eC

Insert

RvLEAM

Tags

AcGFP1

Expression

Mammalian

Promoter

CMV

Available Since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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pZH517

Plasmid

#102670

Purpose

Tetracycline inducible expression of GFPmut2 using autoregulation; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

Depositor

Zach Hensel

Article

Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

Insert

GFPmut2

Expression

Bacterial

Available Since

Dec. 5, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
p2T-CMV-evoCjCas9-eAID-BlastR

Plasmid

#194062

Purpose

Mammalian expression of evoCjCas9 eAID

Depositor

Gerald Schwank

Article

Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

Insert

evoCjCas9-eAID

Use

CRISPR

Expression

Mammalian

Available Since

Sept. 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR macroH2A1.2-C

Plasmid

#30512

Depositor

John Gurdon

Article

Pasque et al EMBO J. 2011 May 6;30(12):2373-87. doi:

Insert

macroH2A1.2-C (Macroh2a1 Mouse)

Use

Gateway - donor vector

Mutation

NO STOP

Available Since

June 25, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
mTFP1-CathespinB-6

Plasmid

#55467

Purpose

Localization: Lysosomes, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

CathespinB

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only
pBSc + MShab

Plasmid

#16146

Depositor

Larry Salkoff

Article

Pak et al J Neurosci. 1991 Mar . 11(3):869-80.

Insert

Mus musculus potassium voltage gated channel (Kcnb1 Mouse, Fly)

Use

Xenopus oocyte expression

Mutation

N/A

Available Since

Jan. 10, 2008

Availability

Academic Institutions and Nonprofits only
pMBA323

Plasmid

#214751

Purpose

To create delta(glmS,nadE) working strains and maintaining their viability

Depositor

Tae Seok Moon

Article

Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

Insert

BBa_J23105-cas9 + pBAD-λ Red genes + ptet-gRNA (pBR322ori)

Use

CRISPR

Expression

Bacterial

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
CP1300-ABEmax

Plasmid

#119810

Purpose

A-to-G base editor

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

Insert

ecTadA-ecTadA*(7.10)-nSpCas9 (CP1300)

Expression

Mammalian

Available Since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAB082h

Plasmid

#79240

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

Ppro1 rpoZ-Cry1Ac-d23C

Expression

Bacterial

Promoter

Ppro1

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-mSmo-WT-V5

Plasmid

#126415

Purpose

Localization, Expression of Smoothened WT in mammalian cell lines.

Depositor

Stacey Ogden

Article

Marada et al PLoS Genet. 2015 Aug 20;11(8):e1005473.

Insert

m-Smoothened (Smo Mouse)

Expression

Mammalian

Available Since

June 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Pinco-mNrp1dCy

Plasmid

#21936

Depositor

Guido Serini

Article

Valdembri et al PLoS Biol. 2009 Jan 27. 7(1):e25.

Insert

neuropilin 1 (Nrp1 Mouse)

Use

Retroviral

Tags

HA and His

Mutation

deleted amino acids 884-923

Available Since

Nov. 10, 2009

Availability

Academic Institutions and Nonprofits only
Pinco-mNrp1dSEA

Plasmid

#21935

Depositor

Guido Serini

Article

Valdembri et al PLoS Biol. 2009 Jan 27. 7(1):e25.

Insert

neuropilin 1 (Nrp1 Mouse)

Use

Retroviral

Tags

HA and His

Mutation

deleted amino acids 920-923

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only
pCK760

Plasmid

#192643

Purpose

Expresses BBa_J23110-sfGFP (capacity monitor) and J3-Bba_J23117-mRFP (CRISPRa reporter) on pSC101-AmpR

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

Insert

BBa_J23110-sfGFP

Use

CRISPR and Synthetic Biology

Promoter

BBa_J23110

Available Since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
CMV+ plasmid with onboard mCherry cassette

Plasmid

#169741

Purpose

CMV+ plasmid that also encodes a separate mCherry cassette. mCherry is used to monitor plasmid dosage.

Depositor

Francois St-Pierre

Article

Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

Insert

eGFP

Use

Synthetic Biology

Expression

Mammalian

Promoter

pCMV-tetO2

Available Since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
YPRCΔ15α1

Plasmid

#101712

Purpose

GoldenBraid vector pDGB2α1, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YPRCΔ15 solo LTR loci

Depositor

Elena Caro Bernat

Article

Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

Type

Empty backbone

Use

Yeast expression

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 11,716 results for: VARS