162,512 results

1. pHR-FUSN-mCh-Cry2WT

   Plasmid

   #101223

   Purpose

   FUS(1-214) fused to mCh-Cry2WT

   Depositor

   Clifford Brangwynne

   Article

   Shin et al Cell. 2017 Jan 12;168(1-2):159-171.e14.

   Insert

   FUS(aa1-214) (FUS Human)
   

   Use

   Lentiviral

   Tags

   mCherry-Cry2

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   Feb. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. Cas9-GFP_sg_mAMPKa1

   Plasmid

   #79004

   Purpose

   Expresses WT Cas9 and GFP, along with a sgRNA to mouse AMPK alpha 1.

   Depositor

   Reuben Shaw

   Article

   Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

   Insert

   Prkaa1 (Prkaa1 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   June 22, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pBeloBac11loxP2272

   Plasmid

   #60342

   Purpose

   This is a BAC vector that contains both wt and mutant loxP sites.

   Depositor

   Jon Coren

   Article

   Coren et al BMC Res Notes. 2017 Jul 28;10(1):344. do

   Type

   Empty backbone

   Use

   Cre/Lox

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. PiggyBac dCas9-KRAB

   Plasmid

   #191314

   Purpose

   piggyBac integration and dox-inducible expression of KRAB-dCas9-HA-tagBFP

   Depositor

   Joanna Wysocka

   Article

   Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

   Inserts

   KRAB-dCas9-HA-tagBFP
   rtTA3-IRES-PURO
   

   Use

   CRISPR

   Tags

   HA and tagBFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. AiP11818-pAAV-AiE2004m_3xC2-minCMV-SYFP2-WPRE3-BGHpA (Alias: CN1818)

   Plasmid

   #164458

   Purpose

   AiE2004m_3xC2 is an optimized enhancer sequence designed to drive AAV-mediated transgene expression in L4/5_IT cortical neurons

   Depositor

   The Allen Institute for Brain Science, Jonathan Ting

   Article

   Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

   Insert

   SYFP2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   minCMV

   Available since

   Feb. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLTR-RD114A

   Plasmid

   #17576

   Depositor

   Jakob Reiser

   Article

   Zhang et al J Virol. 2004 Feb . 78(3):1219-29.

   Insert

   RD114 envelope glycoprotein
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 14, 2008

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. 3xAP1pGL3 (3xAP-1 in pGL3-basic)

   Plasmid

   #40342

   Depositor

   Alexander Dent

   Article

   Vasanwala et al J Immunol. 2002 Aug 15;169(4):1922-9.

   Insert

   3xAP-1
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Contains three canonical AP-1 binding sites (TGAC…

   Promoter

   Available since

   Sept. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. Cas9-GFP_sg_mAMPKa2

   Plasmid

   #79005

   Purpose

   Expresses WT Cas9 and GFP, along with a sgRNA to mouse AMPK alpha 2.

   Depositor

   Reuben Shaw

   Article

   Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

   Insert

   Prkaa2 (Prkaa2 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   June 22, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pGP-JFRC59-13XLexAop2-IVS-Syn21-RSET-jGCaMP8m-p10

   Plasmid

   #218863

   Purpose

   LexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

   Depositor

   GENIE Project

   Article

   Reagents et al figshare Dataset. 2024. doi: 10.6084/m9.

   Insert

   RSET-jGCaMP8m
   

   Use

   Tags

   6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…

   Expression

   Insect

   Mutation

   A25G F286Y

   Promoter

   hsp70

   Available since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAK_Tol2_TRE_Puro

    Plasmid

    #130259

    Purpose

    Tol2 doxycycline inducible expression vector

    Depositor

    Michael Yaffe

    Type

    Empty backbone

    Use

    Tol2 transposon, doxycycline inducible

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE Promoter

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. piggyBac-rtTA (4th_Gen)-NFIB-SOX9-IRES-mKate2 (VK_1244)

    Plasmid

    #209082

    Purpose

    NFIB and SOX9 mediated astrocye induction

    Depositor

    Vikram Khurana

    Article

    Lam et al bioRxiv 2022.11.08.515615

    Inserts

    NFIB-P2A-SOX9-V5 (NFIB, SOX9 Human)
    mKate2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE4G

    Available since

    Dec. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMRE-Tn7-151

    Plasmid

    #118565

    Purpose

    miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mTurquoise2
    

    Use

    Minitn7 delivery vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-sgAMPKa2-Cas9-GFP

    Plasmid

    #208050

    Purpose

    Lentiviral vector expressing Cas9 and an sgRNA targeting AMPKa2

    Depositor

    Naama Kanarek

    Article

    Maynard et al Sci Adv. 2024 Feb 2;10(5):eadj9479. doi:

    Insert

    sgPRKAA2 (PRKAA2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLenti-sgAMPKa1-Cas9-GFP

    Plasmid

    #208049

    Purpose

    Lentiviral vector expressing Cas9 and an sgRNA targeting AMPKa1

    Depositor

    Naama Kanarek

    Article

    Maynard et al Sci Adv. 2024 Feb 2;10(5):eadj9479. doi:

    Insert

    sgPRKAA1 (PRKAA1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. E-cadherin-GFP

    Plasmid

    #28009

    Depositor

    Jennifer Stow

    Article

    Miranda et al J Biol Chem. 2001 Jun 22. 276(25):22565-

    Insert

    E-cadherin (CDH1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. N-Cadherin-EGFP

    Plasmid

    #18870

    Depositor

    Valeri Vasioukhin

    Article

    Nechiporuk et al Dev Cell. 2007 Sep . 13(3):338-50.

    Insert

    N-Cadherin (Cdh2 Mouse)
    

    Use

    Tags

    EGFP, Kanamycin, and N-Cadherin

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 13, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEGFP-C1-ADRP

    Plasmid

    #87161

    Purpose

    Fluorescent protein localized to lipid droplets (LDs)

    Depositor

    Elina Ikonen

    Article

    Salo et al EMBO J. 2016 Dec 15;35(24):2699-2716. Ep

    Insert

    ADRP (PLIN2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPSU150

    Plasmid

    #173836

    Purpose

    Expresses 150 kD Penn State ladder protein

    Depositor

    Song Tan

    Article

    Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

    Insert

    STRHSTPABMBPpepN
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. SPLICS LY-MT Long P2A

    Plasmid

    #213613

    Purpose

    Detect the long-range Lysosome-Mitochondria contact

    Depositor

    Tito Calì

    Article

    Giamogante et al Nat Commun. 2024 Feb 19;15(1):1516. doi:

    Insert

    SPLICS LY-MT Long P2A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV-PEmax-P2A-GFP

    Plasmid

    #180020

    Purpose

    Mammalian expression of SpCas9 PEmax prime editor with P2A-EGFP marker

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    PEmax-P2A-EGFP
    

    Use

    Tags

    P2A-EGFP, SV40 bpNLS, and c-Myc NLS

    Expression

    Mammalian

    Mutation

    Detailed in manuscript

    Promoter

    CMV

    Available since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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