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pBABE-hygro CDK4 R24C

Plasmid

#11254

Depositor

Bob Weinberg

Publication

Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

Insert

CDK4 (CDK4 Human)

Use

Retroviral

Expression

Mammalian

Mutation

R24C

Available since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pT7-PE7 for IVT

Plasmid

#214813

Purpose

Template for in vitro transcription of PE7

Depositor

Brittany Adamson

Publication

Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

Insert

PE7

Use

Template for in vitro transcription

Tags

SV40 bpNLS and c-Myc NLS

Promoter

T7 (inactivated)

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDY1052 PASTEv3 pCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT

Plasmid

#179105

Purpose

pCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Publication

Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

Insert

pCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT

Use

CRISPR

Expression

Mammalian

Available since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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Fuw-dCas9-Tet1CD_IM

Plasmid

#84479

Purpose

Lentiviral construct to express dCas9 fused with an inactive catalytic domain of Tet1 (H1672Y, D1674A)

Depositor

Rudolf Jaenisch

Publication

Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

Insert

dCas9-Tet1CD_IM

Use

Lentiviral

Expression

Mammalian

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pAAV hSyn FLEx mGFP-2A-Synaptophysin-mRuby

Plasmid

#71760

Purpose

mGFP and Synaptophysin-mRuby expression from Cre-expressing neurons

Depositor

Liqun Luo

Publication

Beier et al Cell. 2015 Jul 30;162(3):622-34. doi: 10

Has service

AAV1

Inserts

mGFP
Synaptophysin

Use

AAV and Cre/Lox

Tags

Palmitoylation signal and mRuby

Available since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

45 Articles

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pEGFP-C1 F-tractin-EGFP

Plasmid

#58473

Purpose

Expresses a cytoplasmic actin filament reporter, the neuronal inositol 1,4,5-triphosphate 3-kinase A actin-binding domain known as F-tractin, on a CMV promoter

Depositor

Dyche Mullins

Publication

Belin et al Bioarchitecture. 2015 Aug 28:1-14.

Insert

F-tractin (Itpka Rat)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

28 Articles

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Proinsulin-NanoLuc in pLX304

Plasmid

#62057

Purpose

Luminescent reporter of insulin secretion

Depositor

David Altshuler

Publication

Burns et al Cell Metab. 2015 Jan 6;21(1):126-37. doi

Insert

Proinsulin-NanoLuc (Ins2 Synthetic, Mouse)

Use

Lentiviral and Luciferase

Expression

Mammalian

Mutation

Inserted NanoLuc into C-peptide of mouse Ins2 cod…

Promoter

CMV

Available since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pAAV-hSyn-DIO-mCherry

Plasmid

#50459

Purpose

Double floxed mCherry under the control of human synapsin promoter

Depositor

Bryan Roth

Has service

AAV1, AAV2, AAV5, AAV8, AAV9, and AAV Retrograde

Insert

mCherry

Use

AAV

Tags

N/A

Promoter

human Synapsin 1

Available since

March 17, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

379 Articles

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pHD-DsRed

Plasmid

#51434

Purpose

Vector for generating dsDNA donors for homology-directed repair. Contains the visible marker 3xP3-DsRed.

Depositor

Kate O'Connor-Giles

Publication

Gratz et al Genetics. 2014 Apr;196(4):961-71. doi: 1

Insert

LoxP-3xP3-DsRed-LoxP

Promoter

3xP3

Available since

Feb. 27, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

56 Articles

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pSimpleII-U6-tracr-U6-BsmBI-NLS-NmCas9-HA-NLS(s)

Plasmid

#47868

Purpose

This plasmid contains expression cassette for NmCas9 with N and C NLS and an HA tag, a cassette for expression of tracrRNA, a cassette for cloning crRNA under the control of U6 promoter.

Depositor

Erik Sontheimer, James Thomson

Publication

Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.

Inserts

NmCas9
U6pr-tracrRNA

Use

CRISPR

Tags

HA and NLS

Expression

Mammalian

Promoter

EF1a and U6

Available since

Sept. 30, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pINDUCER11 (miR-RUG)

Plasmid

#44363

Purpose

Inducible lentiviral gene silencing vector. Insert PheSGly294, (XhoI to EcoR1; 1.4kb), can be replaced w mir30 based hairpin of interest

Depositor

Thomas Westbrook

Publication

Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

Insert

PheS Gly294

Use

Lentiviral

Available since

Aug. 15, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pRK-ATF3

Plasmid

#26115

Depositor

Yihong Ye

Publication

Wang et al Proc Natl Acad Sci U S A. 2009 Feb 17. 1

Insert

ATF3 (ATF3 Human)

Tags

FLAG

Expression

Mammalian

Available since

Dec. 2, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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eIF2a 3

Plasmid

#21809

Depositor

David Ron

Insert

eIF2a (EIF2S1 Human)

Expression

Mammalian

Mutation

S52D

Available since

Sept. 10, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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aMHC-mCherry-Rex-Blasticidin

Plasmid

#21228

Depositor

Mark Mercola

Publication

Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

Insert

alpha myosin heavy chain promoter

Use

Lentiviral

Tags

mCherry

Available since

June 30, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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LRRK2-WT

Plasmid

#17609

Depositor

Ted Dawson

Publication

West et al Proc Natl Acad Sci U S A. 2005 Nov 15;10

Insert

LRRK2 (LRRK2 Human)

Tags

His and Myc

Expression

Mammalian

Available since

March 31, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pBAC-BA-lacZ

Plasmid

#13423

Depositor

Keith Joung

Publication

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

BA

Use

Cre/Lox

Expression

Bacterial

Available since

Feb. 8, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pLenti6-DEST PINK1-V5 WT

Plasmid

#13320

Depositor

Mark Cookson

Publication

Beilina et al Proc Natl Acad Sci U S A. 2005 Apr 19. 1

Insert

PINK1 (PINK1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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pMKO.1 puro GFP shRNA

Plasmid

#10675

Purpose

Negative control vector for pMKO.1 puro (Addgene plasmid #8452); Contains shRNA against GFP.

Depositor

William Hahn

Publication

Masutomi et al Proc Natl Acad Sci U S A. 2005 Jun 7. 10

Insert

GFP shRNA

Use

RNAi and Retroviral

Expression

Mammalian

Available since

Jan. 6, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pBABE-FLAG-LKB1

Plasmid

#8592

Depositor

Lewis Cantley

Publication

Shaw et al Proc Natl Acad Sci U S A 2004 Mar 9;101(

Insert

LKB1 (STK11 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Available since

July 18, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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pRSETB/mStayGold

Plasmid

#212017

Purpose

mStayGold has an n1 adaptor at its N-terminus and is useful for C-terminal tagging. This construct is used also for expression of mStayGold itself.

Depositor

Atsushi Miyawaki

Publication

Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Type

Empty backbone

Tags

6xHis, T7 tag, Xpress tag, mStayGold

Expression

Bacterial

Promoter

T7

Available since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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