162,473 results

1. pAAV-hSYNCMV tAKARα

   Plasmid

   #119921

   Purpose

   AAV construct with tAKARα being expressed under the human synapsin promotor with WPRE enhancer.

   Depositor

   Haining Zhong

   Article

   Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do

   Insert

   tAKARα
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAGGS-PKCdelta-EGFP

   Plasmid

   #178856

   Purpose

   Mammalian expression of PKCδ fused to EGFP

   Depositor

   Shinya Tsukiji

   Article

   Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

   Insert

   PKCδ (PRKCD Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   July 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJL1-sfGFP

   Plasmid

   #102634

   Purpose

   In vitro expression of sfGFP from the T7 promoter

   Depositor

   Michael Jewett

   Article

   Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

   Insert

   sfGFP
   

   Use

   Tags

   strep tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. σ1-mCherry

   Plasmid

   #186578

   Purpose

   Expression of adaptor protein 1 (AP1) sigma subunit AP1S1 tagged with mCherry

   Depositor

   Stephen Royle

   Article

   Kuey et al Elife. 2022 Jul 19;11. pii: 78929. doi:

   Insert

   AP1S1 (AP1S1 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-SynaptoTAG2

   Plasmid

   #175275

   Purpose

   AAV vector for mapping synaptic projections of infected neurons; expresses tdTomato to reveal neuronal soma and axons, and expresses EGFP fused to Synaptobrevin-2 to track synaptic terminals.

   Depositor

   Wei Xu

   Article

   Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

   Insert

   EGFP-Synaptobrevin-2; tdTomato (Vamp2 )
   

   Use

   AAV

   Tags

   EGFP and tdTomato (P2A cleavage)

   Expression

   Mutation

   Promoter

   Synapsin

   Available since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV2 ITR_phU6_gRNA scaffold-DMD sg1_phU6_gRNA scaffold-DMD sg16_pMHCK7-3x Flag-NLS-enOsCas12f1-NLS_pA_AAV2 ITR

   Plasmid

   #197028

   Purpose

   AAV vector encoding a human codon-optimized enOsCas12f1 driven by MHCK7 promoter, DMD-targeting guide RNAs compatible with enOsCas12f1 driven by hU6

   Depositor

   Xiangfeng Kong

   Article

   Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:

   Insert

   humanized enOsCas12f1
   

   Use

   AAV

   Tags

   Expression

   Mutation

   D52R / T132R

   Promoter

   MHCK7, hU6

   Available since

   May 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. ADRA1A-Tango

   Plasmid

   #66213

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   ADRA1A (ADRA1A Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-GFP

   Plasmid

   #11150

   Purpose

   Mammalian expression vector for expression of GFP (CAG promoter)

   Depositor

   Connie Cepko

   Article

   Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

   Has Service

   Cloning Grade DNA

   Insert

   EGFP from Aequorea victoria
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPGK-T7/2-CD44v2-10 (fl)

   Plasmid

   #137824

   Purpose

   expression of CD44 proteins in mammalian cells

   Depositor

   Ursula Gunthert

   Article

   Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

   Insert

   CD44v2-10 (fl) (CD44 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   full length and mutations A282T, T483A, N535D, S5…

   Promoter

   PGK

   Available since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pZR112_Lenti-SFFV-mCherry-2A-dCas9-VP64

    Plasmid

    #180263

    Purpose

    Lentiviral expression vector for dCas9-VP64 in mammalian cells

    Depositor

    Alexander Marson

    Article

    Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Nuclease dead Cas9

    Promoter

    SFFV

    Available since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. LV-hNG2-GFP

    Plasmid

    #183910

    Purpose

    Expression of GFP under the human NG2 promoter

    Depositor

    Chun-Li Zhang

    Article

    Tai et al Cell Stem Cell. 2021 May 6;28(5):923-937

    Insert

    GFP (GFAP Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. iF59 PB-TO-oNgn2-puro

    Plasmid

    #204734

    Purpose

    Improved Dox-inducible neurogenin-2; Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression. oNGN2 is a Phospho-mutant which avoids inhibition by cdk kinase.

    Depositor

    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

    Insert

    Optimized neurogenin-2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pWMD23

    Plasmid

    #140006

    Purpose

    Expression of ORF1 (Shuffle 1 NLS) and Pong TPase (L418A, L420A)

    Depositor

    Charles Nathan Hancock

    Article

    Johnson et al Plant Direct. 2021 Jan 19;5(1):e00300. d

    Inserts

    ORF1 Shuffle 1 NLS
    Pong TPase LA
    

    Use

    Tags

    Expression

    Plant

    Mutation

    NES sequence mutated L418A, L420A and Ping N-term…

    Promoter

    Available since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEF_dCas9

    Plasmid

    #68416

    Purpose

    Transient expression of Sp-dCas9 in mammalian cells, under an EF1-alpha promoter.

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D10A/H841A

    Promoter

    human EF1[alpha]

    Available since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. EGFR-mApple

    Plasmid

    #203773

    Purpose

    Number and brightness analysis of EGFR oligomerization

    Depositor

    Thorsten Wohland

    Article

    Sankaran et al Nat Commun. 2021 Mar 19;12(1):1748. doi:

    Insert

    EGFR (EGFR Human)
    

    Use

    Tags

    mApple

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. TFORF0033

    Plasmid

    #141485

    Purpose

    Lentiviral vector for overexpressing the SP1 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    SP1 (SP1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDestTol2pA2-9xGRE

    Plasmid

    #74736

    Purpose

    GRE-dependent EGFP reporter construct flanked by the minimal Tol2 transposon elements

    Depositor

    Luisa Dalla Valle

    Article

    Benato et al Mol Cell Endocrinol. 2014 Jul 5;392(1-2)

    Insert

    EGFP
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    nine GC-responsive elements (9xGRE) from the rat …

    Available since

    April 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. GFP-SMARCA4

    Plasmid

    #65391

    Purpose

    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

    Depositor

    Kyle Miller

    Article

    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

    Insert

    SMARCA4 (SMARCA4 Human)
    

    Use

    Tags

    EmGFP and V5

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti_dCas9-KRAB-MeCP2_Blast

    Plasmid

    #221501

    Purpose

    Lentiviral construct for expression of the dCas9-KRAB-MeCP2 CRISPRi vector

    Depositor

    Matthew Meyerson

    Article

    Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

    Insert

    dCas9-KRAB-MeCP2-T2A-Blast
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

    Promoter

    CMV

    Available since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-hSyn-PSDΔ1,2-FRBx3

    Plasmid

    #225827

    Purpose

    A component of SYNCit-K (Synapse-targeted chemically induced translocation of Kalirin-7)

    Depositor

    Haruo Kasai

    Article

    Sawada et al Science. 2024 Sep 27;385(6716):1459-1465

    Insert

    PSDΔ1,2-FRBx3
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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