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Fuw-dCas9-Tet1CD_IM

Plasmid

#84479

Purpose

Lentiviral construct to express dCas9 fused with an inactive catalytic domain of Tet1 (H1672Y, D1674A)

Depositor

Rudolf Jaenisch

Publication

Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

Insert

dCas9-Tet1CD_IM

Use

Lentiviral

Expression

Mammalian

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pSRK2706

Plasmid

#78571

Purpose

Expresses untagged human rhinovirus 3C protease in E. coli

Depositor

David Waugh

Publication

Raran-Kurussi et al Anal Biochem. 2016 Apr 19;504:30-37. doi

Insert

human rhinovirus 3C protease (HRVBgp1 Human)

Expression

Bacterial

Promoter

T7

Available since

June 16, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pLenti-sgRNA

Plasmid

#71409

Purpose

There is no gene/insert. It is a backbone plasmid that others can use to insert sgRNAs of interest. The cloning site for this BsmBI

Depositor

Eric Lander, David Sabatini

Type

Empty backbone

Use

Lentiviral

Available since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

20 Articles

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pAAV hSyn FLEx mGFP-2A-Synaptophysin-mRuby

Plasmid

#71760

Purpose

Expression of membrane-tethered EGFP and Synaptophysin-mRuby from Cre-expressing neurons

Depositor

Liqun Luo

Publication

Beier et al Cell. 2015 Jul 30;162(3):622-34. doi: 10

Has service

AAV1

Inserts

EGFP
Synaptophysin

Use

AAV and Cre/Lox

Tags

Palmitoylation signal and mRuby

Available since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

47 Articles

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Proinsulin-NanoLuc in pLX304

Plasmid

#62057

Purpose

Luminescent reporter of insulin secretion

Depositor

David Altshuler

Publication

Burns et al Cell Metab. 2015 Jan 6;21(1):126-37. doi

Insert

Proinsulin-NanoLuc (Ins2 Synthetic, Mouse)

Use

Lentiviral and Luciferase

Expression

Mammalian

Mutation

Inserted NanoLuc into C-peptide of mouse Ins2 cod…

Promoter

CMV

Available since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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p21-Reporter

Plasmid

#52432

Purpose

DNA damage inducible Red Fluorescent Protein for mammalian expression

Depositor

Joel Voldman

Publication

Fendyur et al Anal Chem. 2014 Aug 5;86(15):7598-605. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

p21-p53 Tandem Response Element Promoter

Tags

TurboRFP

Expression

Mammalian

Promoter

13X repeated p21-p53 response element

Available since

Oct. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pSimpleII-U6-tracr-U6-BsmBI-NLS-NmCas9-HA-NLS(s)

Plasmid

#47868

Purpose

This plasmid contains expression cassette for NmCas9 with N and C NLS and an HA tag, a cassette for expression of tracrRNA, a cassette for cloning crRNA under the control of U6 promoter.

Depositor

Erik Sontheimer, James Thomson

Publication

Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.

Inserts

NmCas9
U6pr-tracrRNA

Use

CRISPR

Tags

HA and NLS

Expression

Mammalian

Promoter

EF1a and U6

Available since

Sept. 30, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pRK-ATF3

Plasmid

#26115

Depositor

Yihong Ye

Publication

Wang et al Proc Natl Acad Sci U S A. 2009 Feb 17. 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ATF3 (ATF3 Human)

Tags

FLAG

Expression

Mammalian

Available since

Dec. 2, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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HIR WT

Plasmid

#24049

Depositor

Frederick Stanley

Publication

Jacob et al Mol Cell Endocrinol. 2002 Jan 15. 186(1)

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Insulin Receptor (INSR Human)

Expression

Mammalian

Available since

Dec. 1, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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aMHC-mCherry-Rex-Blasticidin

Plasmid

#21228

Depositor

Mark Mercola

Publication

Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

Insert

alpha myosin heavy chain promoter

Use

Lentiviral

Tags

mCherry

Available since

June 30, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pAdEasy-2

Plasmid

#16401

Purpose

Adenoviral vector for use in AdEasy System, for use with large inserts.

Depositor

Bert Vogelstein

Publication

He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

Type

Empty backbone

Use

Adenoviral

Expression

Mammalian

Available since

June 11, 2008

Availability

Academic Institutions and Nonprofits only

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LRRK2-WT

Plasmid

#17609

Depositor

Ted Dawson

Publication

West et al Proc Natl Acad Sci U S A. 2005 Nov 15;10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

LRRK2 (LRRK2 Human)

Tags

His and Myc

Expression

Mammalian

Available since

March 31, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pcDNA3-DN-hCUL1-FLAG

Plasmid

#15818

Depositor

Wade Harper

Publication

Jin et al Methods Enzymol. 2005;399:287-309. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cullin1 (CUL1 Human)

Tags

Flag

Expression

Mammalian

Mutation

Dominant negative: N452

Available since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pBAC-BA-lacZ

Plasmid

#13423

Depositor

Keith Joung

Publication

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

BA

Use

Cre/Lox

Expression

Bacterial

Available since

Feb. 8, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pBABE-FLAG-LKB1

Plasmid

#8592

Depositor

Lewis Cantley

Publication

Shaw et al Proc Natl Acad Sci U S A 2004 Mar 9;101(

Insert

LKB1 (STK11 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Available since

July 18, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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pZR112_Lenti-SFFV-mCherry-2A-dCas9-VP64

Plasmid

#180263

Purpose

Lentiviral expression vector for dCas9-VP64 in mammalian cells

Depositor

Alexander Marson

Publication

Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

Insert

dCas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

Nuclease dead Cas9

Promoter

SFFV

Available since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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ABE8e(TadA-8e V106W)

Plasmid

#138495

Purpose

A-to-G base editor

Depositor

David Liu

Publication

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ecTadA(8e V106W)-nSpCas9

Expression

Mammalian

Mutation

TadA-8e V106W

Available since

March 17, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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FUW-tetO-MCS

Plasmid

#84008

Purpose

doxycycline-inducible lentiviral vector

Depositor

Stefano Piccolo

Publication

Panciera et al Cell Stem Cell. 2016 Sep 9. pii: S1934-5

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

TRE-CMV

Available since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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pEZT-BM

Plasmid

#74099

Purpose

Multi-purpose rapid titer bacmam expression vector

Depositor

Ryan Hibbs

Publication

Morales-Perez et al Structure. 2016 Mar 24. pii: S0969-2126(

Type

Empty backbone

Use

Bacmam, baculovirus

Tags

EGFP following p10 promoter

Expression

Mammalian

Promoter

CMV, p10

Available since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

31 Articles

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pLEGFP-WT-STAT3

Plasmid

#71450

Purpose

Retroviral expression of WT-STAT3. Please note that this plasmid contains WT-STAT3 tagged with FLAG, not GFP.

Depositor

George Stark

Publication

Dasgupta et al J Biol Chem. 2014 Oct 31;289(44):30763-7

Insert

STAT3 (STAT3 Human)

Use

Retroviral

Tags

FLAG

Promoter

CMV

Available since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

19 Articles

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