We narrowed to 4,881 results for: phy

1. pBAD_His B_QuasAr1

   Plasmid

   #64135

   Purpose

   Fluorescent reporter for membrane voltage with improved brightness

   Depositor

   Adam Cohen

   Article

   Hochbaum et al Nat Methods. 2014 Aug;11(8):825-33. doi:

   Insert

   QuasAr1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pME-CreLite

   Plasmid

   #131782

   Purpose

   Middle entry vector (Tol2 kit) with bi-cistronic CreLite system components, PhyBΔCreC and PIF6CreN, linked by P2A.

   Depositor

   George Eisenhoffer

   Article

   Yen et al Dev Dyn. 2020 Nov;249(11):1394-1403. doi

   Insert

   PhyBCreC-P2A-PIF6CreN
   

   Use

   Cre/Lox; Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFL-EGFP-3xFlag-Fml1

   Plasmid

   #193058

   Purpose

   Baculovirus expression of S.pombe Fml1 for recombinant protein production

   Depositor

   Andrew Deans

   Article

   Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:

   Insert

   Fml1 (fml1 Fission Yeast)
   

   Use

   Tags

   3xFlag

   Expression

   Insect

   Mutation

   Promoter

   polyhedron

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFE-hCA

   Plasmid

   #132999

   Purpose

   pFE carrying a human carbonic anhydrase II gene (Uniprot: P00918)

   Depositor

   David Savage

   Article

   Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

   Insert

   hCAII (CA2 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tetR

   Available since

   Nov. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLJC6-GPT1-3xFLAG

   Plasmid

   #163448

   Purpose

   lentiviral overexpression vector for human GPT1

   Depositor

   Jason Cantor

   Article

   Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

   Insert

   GPT1 (GPT Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   See: comments

   Promoter

   Ubc

   Available since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. MAC_C_FGFR2

   Plasmid

   #187751

   Purpose

   MAC-tagged gene expression of human FGFR2

   Depositor

   Markku Varjosalo

   Article

   Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

   Insert

   FGFR2 (FGFR2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. attB-­GFP-­Poly(A)‐NeoR

   Plasmid

   #65526

   Purpose

   NeoR containing plasmid for the generation of gene disruptions via Bxb1-mediated recombination into MIN-tagged cell lines. This vector can also be used to express cDNAs.

   Depositor

   Sebastian Bultmann

   Article

   Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

   Insert

   attB-GFP
   

   Use

   Mouse Targeting; Bxb1

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCDNA3-human C-MYC-V394D

   Plasmid

   #74162

   Purpose

   Mammalian expression of human cMyc VD mutant

   Depositor

   Martine Roussel

   Article

   Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

   Insert

   cMyc (MYC Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   V394D

   Promoter

   CMV

   Available since

   April 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET-51b(+)-Caprola_14

   Plasmid

   #194675

   Purpose

   Protein production of the split HaloTag based calcium recorder Caprola_14 in bacteria

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Caprola_14
   

   Use

   Tags

   His-tag and Strep-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET-51b(+)-Caprola_15

    Plasmid

    #194676

    Purpose

    Protein production of the split HaloTag based calcium recorder Caprola_15 in bacteria

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_15
    

    Use

    Tags

    His-tag and Strep-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCDNA5/FRT_CMV_NES-Caprola_04-mEGFP

    Plasmid

    #194682

    Purpose

    CMV driven expression of the calcium recorder Caprola_04 in mammalian cell lines fused to mEGFP

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_04-mEGFP
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCDNA5/FRT_CMV_NES-Caprola_06-mEGFP

    Plasmid

    #194684

    Purpose

    CMV driven expression of the calcium recorder Caprola_06 in mammalian cell lines fused to mEGFP

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_06-mEGFP
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. psgRNA (GB0645)

    Plasmid

    #68210

    Purpose

    Provides a Short guide RNA which is the combination of the bacterial crRNA and tracrRNA into a single guide transcript; it does not contain any target site

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    SgRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Oct. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGS1034

    Plasmid

    #63796

    Purpose

    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal CBP-3xFLAG-TEV-ProtA triple tag and a LEU2 selection marker

    Depositor

    Bjoern Sander, Monika Golas

    Article

    Lin et al Biochim Biophys Acta. 2015 Mar;1854(3):1

    Insert

    CBP-3xFLAG-TEV-Protein A followed by KlLEU2
    

    Use

    E. coli cloning vector

    Tags

    CBP-3xFLAG-TEV-Protein A

    Expression

    Mutation

    Promoter

    none

    Available since

    April 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA-D2s-L-V2

    Plasmid

    #19968

    Depositor

    Jonathan Javitch

    Article

    Guo et al EMBO J. 2008 Sep 3. 27(17):2293-304.

    Insert

    D2 short variant-C terminal Venus (DRD2 Human, Aequorea Victoria)
    

    Use

    Tags

    C terminal Venus and Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

16. pEGFP-C1-KSENB-Nup133-mut

    Plasmid

    #87339

    Purpose

    To express human Nup133 in mammalian cells. It contains synonymous mutations that are not targeted by shRNA

    Depositor

    Lei Lu

    Article

    Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

    Insert

    NUP133 (NUP133 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    shRNA resistant changes; deletion of Nup133 AA1-7

    Promoter

    CMV

    Available since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. MAC_C_ERBB2

    Plasmid

    #187776

    Purpose

    MAC-tagged gene expression of human ERBB2

    Depositor

    Markku Varjosalo

    Article

    Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

    Insert

    ERBB2 (ERBB2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMSCVneo-HOXB3-FLAG

    Plasmid

    #74508

    Purpose

    Retroviral expression of HOXB3

    Depositor

    Lars Rönnstrand

    Article

    Lindblad et al Biochem Biophys Res Commun. 2015 Nov 27;

    Insert

    HOXB3 (HOXB3 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    unknown

    Available since

    Dec. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

19. EGFR∆550-580-mEGFP

    Plasmid

    #203788

    Purpose

    Number and brightness analysis of EGFR oligomerization

    Depositor

    Thorsten Wohland

    Article

    Balasubramanian et al Biophys J. 2022 Dec 6;121(23):4452-4466.

    Insert

    EGFR (EGFR Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    aa 550-580 deleted

    Promoter

    Available since

    July 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. DJ-1 TEV site pET15b

    Plasmid

    #60687

    Purpose

    Recombinant protein expression of Park7

    Depositor

    Mark Wilson

    Article

    Milkovic et al Protein Sci. 2015 Oct;24(10):1671-85. do

    Insert

    DJ-1 (PARK7 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    TEV site was inserted (see comments below).

    Promoter

    T7

    Available since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

21. pCD86-mApple

    Plasmid

    #133859

    Purpose

    Mammalian expression of CD86 C-terminally tagged with mApple

    Depositor

    Dave Piston

    Article

    Foust et al Biophys J. 2019 Nov 5;117(9):1764-1777.

    Insert

    CD86 (CD86 Human)
    

    Use

    Tags

    mApple

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pPKm-196

    Plasmid

    #90511

    Purpose

    pcDNA3 - CMVmin PIF6-DBD, expressing PIF6-DBD under CMV promoter

    Depositor

    Todd Coleman

    Article

    Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

    Insert

    PIF6-DBD
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET29a-hNABP1-C

    Plasmid

    #128311

    Purpose

    Express hNABP1 C-terminus (125–204 aa) in E. coli with a C-terminal His-tag

    Depositor

    Yuliang Wu

    Article

    Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

    Insert

    Human nucleic acid-binding protein 1 (hNABP1)-C terminus (125-204 aa) (NABP1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pRK5-myc-Miro2 R425V

    Plasmid

    #47898

    Purpose

    Expresses myc tagged Miro2 R425V constitutively active mutant

    Depositor

    Pontus Aspenström

    Article

    Fransson et al Biochem Biophys Res Commun. 2006 Jun 2;3

    Insert

    Miro2 R425V (RHOT2 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    R425V, constitutively active mutant

    Promoter

    CMV

    Available since

    Sept. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

25. pSW554 - pOp5:SP-mVenus-HDEL

    Plasmid

    #116006

    Purpose

    destination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expression

    Depositor

    Sebastien Wolf

    Article

    Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

    Insert

    pOp5:SP(ER)-mVenus-HDEL:tUBQ10::SulfR
    

    Use

    Tags

    HDEL and SP(ER)

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pRK5-myc-Miro2 S430N

    Plasmid

    #47899

    Purpose

    Expresses myc tagged Miro2 S430N dominant negative mutant

    Depositor

    Pontus Aspenström

    Article

    Fransson et al Biochem Biophys Res Commun. 2006 Jun 2;3

    Insert

    Miro2 S430N (RHOT2 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    S430N, dominant negative mutant

    Promoter

    CMV

    Available since

    Sept. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

27. pCDNA6-LRRC52-V5-Flag-His

    Plasmid

    #127818

    Purpose

    Expression of human LRRC52 in mammalian cells

    Depositor

    Jiusheng Yan

    Article

    Li et al J Gen Physiol. 2015 Jun;145(6):543-54. d

    Insert

    LRRC52 (LRRC52 Human)
    

    Use

    Tags

    V5, FLAG, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pJRTCLCrVA010

    Plasmid

    #22843

    Depositor

    Niki Robertson

    Article

    Tuttle et al Plant Physiol. 2008 Sep . 148(1):41-50.

    Insert

    A DNA containing GFP
    

    Use

    Cotton expression vector

    Tags

    Expression

    Bacterial

    Mutation

    Cotton Leaf Crumple Virus A component with Coat P…

    Promoter

    Available since

    June 14, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. attB-­mCh-­Poly(A)‐NeoR

    Plasmid

    #65528

    Purpose

    NeoR containing plasmid for the generation of gene disruptions via Bxb1-mediated recombination into MIN-tagged cell lines. This vector can also be used to express cDNAs.

    Depositor

    Sebastian Bultmann

    Article

    Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

    Insert

    attB-mCherry
    

    Use

    Mouse Targeting; Bxb1

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA1-GATA4-opposite orientation

    Plasmid

    #51928

    Purpose

    for T7 directed antisense production of mouse GATA4

    Depositor

    Jeffery Molkentin

    Article

    Liang et al J Biol Chem. 2001 Aug 10;276(32):30245-5

    Insert

    GATA4 reverse orientation (Gata4 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 28, 2014

    Availability

    Academic Institutions and Nonprofits only

31. attB‐mCh­‐Poly(A)-­PuroR

    Plasmid

    #65529

    Purpose

    PuroR containing plasmid for the generation of gene disruptions via Bxb1-mediated recombination into MIN-tagged cell lines. This vector can also be used to express cDNAs.

    Depositor

    Sebastian Bultmann

    Article

    Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

    Insert

    attB-mCherry
    

    Use

    Mouse Targeting; Bxb1

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pTRE-FDXR mut

    Plasmid

    #16611

    Depositor

    Bert Vogelstein

    Article

    Hwang et al Nat Med. 2001 Oct . 7(10):1111-7.

    Insert

    FDXR (FDXR Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Deletion mutant, contains aa 1-42

    Promoter

    Available since

    April 7, 2008

    Availability

    Academic Institutions and Nonprofits only

33. pEGB2alpha2 35s:hCas9:tNos (GB0639)

    Plasmid

    #68222

    Purpose

    Transcriptional unit for (human codon optimized) Cas9 plant expression driven by the 35S promoter

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    hCas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    35S

    Available since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAMV:nAChRgamma-eGFP

    Plasmid

    #29693

    Depositor

    Henry Lester

    Article

    Pantoja et al Biophys J. 2009 Jan . 96(1):226-37.

    Insert

    GFP tagged Mus musculus cholinergic receptor, nicotinic, gamma (Chrng Mouse)
    

    Use

    Tags

    AMV promotor and poly(A) tail

    Expression

    Bacterial

    Mutation

    eGFP inserted after position S380

    Promoter

    Available since

    July 16, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCDH-EF1-mouse SmyD1-T2A-copGFP

    Plasmid

    #101157

    Purpose

    Lentiviral expression of SmyD1 and copGFP

    Depositor

    Martine Roussel

    Article

    Wei et al Oncotarget. 2017 Jul 27. doi: 10.18632/o

    Insert

    SmyD1 (Smyd1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available since

    Oct. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pLJC2-UMPS (Y37A, R155A)-3xFLAG

    Plasmid

    #87976

    Purpose

    Expression of UMPS (Y37A, R155A) in mammalian cells

    Depositor

    David Sabatini

    Article

    Cantor et al Cell. 2017 Apr 6;169(2):258-272.e17. doi

    Insert

    UMPS (Y37A, R155A) (UMPS Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Y37A, R155A

    Promoter

    CMV

    Available since

    April 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pLJC2-Rap2A-3xFLAG

    Plasmid

    #87974

    Purpose

    3rd generation lentiviral vector for Expression of Rap2A in mammalian cells

    Depositor

    David Sabatini

    Article

    Cantor et al Cell. 2017 Apr 6;169(2):258-272.e17. doi

    Insert

    RAP2A (RAP2A Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pVL94 - pOp4:VND7

    Plasmid

    #116016

    Purpose

    destination vector for GreenGate cloning method, "Effector line", crossed with a "Driver line" the GOI, here VND7, will be expressed upon Dex-induction

    Depositor

    Thomas Greb

    Article

    Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

    Insert

    pOp4:B-dummy:VND7:VP16:tUBQ10::BastaR
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pFRT-TODestFLAGHA_RBPMS_KDR_HindIII

    Plasmid

    #65662

    Purpose

    FLAG-HA expression vector with RBPMS carrying mutation to introduce HindIII site and encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)

    Depositor

    Thomas Tuschl

    Article

    Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

    Insert

    RBPMS (RBPMS Human)
    

    Use

    Tags

    FLAG HA

    Expression

    Mammalian

    Mutation

    introduced HindIII site (final goal to exchange F…

    Promoter

    CMV, 2x Tet

    Available since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pB80-SSPB(micro)-GFP-ppKin14VIb(861-1321)

    Plasmid

    #174639

    Purpose

    Optogenetic coupling to dimeric moss kinesin-14 for retrograde transport via SSPB(micro)

    Depositor

    Lukas Kapitein

    Article

    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    SSPB(micro)-GFP-ppKinVIb(861-1321)
    

    Use

    Tags

    SSPB(micro)-GFP

    Expression

    Mammalian

    Mutation

    SSPB:Arg73Gln; EGFP: Met1Del; ppKin14VIb(aa861-13…

    Promoter

    Chicken beta-actin

    Available since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge