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We narrowed to 7,002 results for: tet

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Showing: 4251 - 4300 of 7002 results

  1. pCas9GG

    Plasmid
    #131009
    Purpose
    Backbone plasmid for generating CRISPR arrays for SpCas9 using CRATES. Contains a direct repeat and a RFP-dropout cassette.
    Depositor
    Chase Beisel
    Article
    Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:
    Inserts
    direct repeat of SpCas9
    promoter PJ23119
    mRFP expression cassette
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    Bba_R0040 TetR
    Available Since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    131009-plasmid-map-sequence-id-257082

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  3. pDule-Acd82

    Plasmid
    #174099
    Purpose
    Acridone82 tRNA synthetase and cognate amber suppressing tRNA derived from M. barkeri for expression in E.coli.
    Depositor
    Ryan Mehl
    Article
    Jones et al Chem Sci. 2021 Aug 3;12(36):11955-11964.
    Insert
    Acridone 82 tRNA synthetase and tRNA
    Expression
    Bacterial
    Mutation
    N311S, C313G, V366A, W382T
    Available Since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    174099-plasmid-map-sequence-id-345716

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  4. pFNC-5

    Plasmid
    #233297
    Purpose
    Plasmid encoding the BxbI phage integrase. It can be used to remove the antibiotic resistance cassette integrated with pCIFR. TcR, easily curable via sucrose counterselection.
    Depositor
    Pablo Ivan Nikel
    Article
    Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1
    Inserts
    BxbI phage integrase
    sacB
    Expression
    Bacterial
    Promoter
    PEM7 and unknown
    Available Since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    233297-plasmid-map-sequence-id-468477

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  9. pNK-TGCK

    Plasmid
    #45359
    Depositor
    Sean Wu
    Inserts
    tTA
    eGFP-Cre
    Expression
    Mammalian
    Promoter
    Mouse Nkx2.5 cardiac enhancer and promoter fragme…
    Available Since
    Aug. 22, 2013
    Availability
    Academic Institutions and Nonprofits only
    45359-plasmid-map-sequence-id-393051

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  12. pFP.R274

    Plasmid
    #138223
    Purpose
    Expresses the split mCherry reporter interrupted by the TvoVMA intein (in cis)
    Depositor
    Baojun Wang
    Article
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    araC (araC )
    mCherryN(GSK).TvoVMA.(TVI)mCherryC.H6
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    P(araBAD) and iPC
    Available Since
    May 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    138223-plasmid-map-sequence-id-296592

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  13. pFP.R371

    Plasmid
    #138241
    Purpose
    Expresses the split mCherry reporter interrupted by the MP-M-DnaB artificially mini-intein (in cis)
    Depositor
    Baojun Wang
    Article
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    araC (araC )
    mCherryN(NGK).MP-M-DnaB*.(TEL)mCherryC.H6
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    P(araBAD) and iPC
    Available Since
    March 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    138241-plasmid-map-sequence-id-273486

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  14. pMflET-o4

    Plasmid
    #101314
    Purpose
    Contains: complete oriC region of M. florum L1 (oriC4 fragment), colE1 rep origin, recoded tetM and ereB resistance genes, origin of transfer of RP4 plasmid.
    Depositor
    Sébastien Rodrigue
    Article
    Matteau et al Appl Environ Microbiol. 2017 Mar 17;83(7
    Inserts
    oriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
    ereB resistance gene
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available Since
    Nov. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    101314-plasmid-map-sequence-id-198386

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  17. HA-mRIPK3 D161N-Flag/cHA-pTRIPZ vector

    Plasmid
    #73705
    Purpose
    lentiviral expression of mRIPK3 mutant
    Depositor
    Francis Chan
    Insert
    RIPK3 (Ripk3 Mouse)
    Use
    Lentiviral
    Tags
    Flag and HA
    Expression
    Mammalian
    Mutation
    D161N
    Promoter
    CMV/tet
    Available Since
    March 25, 2016
    Availability
    Academic Institutions and Nonprofits only

  18. MLM3709

    Plasmid
    #49943
    Purpose
    Expresses a 3x Flag TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding site
    Depositor
    Keith Joung
    Article
    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038
    Insert
    3x FLAG Tet1CD RH-3 (RHOXF2 Human)
    Use
    TALEN
    Tags
    3x FLAG
    Promoter
    EF1a
    Available Since
    Dec. 20, 2013
    Availability
    Academic Institutions and Nonprofits only

  19. pAL1

    Plasmid
    #197285
    Purpose
    Shuttle plasmid that replicates in E. coli, S. cerevisiae, and A. laidlawii. Contains the OriC from A. laidlawii 8195
    Depositor
    Bogumil Karas
    Article
    Nucifora et al ACS Synth Biol. 2024 Jan 19;13(1):45-53.
    Insert
    Origin of replication of A. laidlawii 8195: whole dnaA gene, plus upstream and downstream intergenic regions
    Use
    Synthetic Biology; Bacterial and yeast cloning
    Available Since
    April 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    197285-plasmid-map-sequence-id-389699

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  20. pFP.R364

    Plasmid
    #138234
    Purpose
    Expresses the split mCherry reporter interrupted by the Cth-Ter artificially mini-intein (in cis)
    Depositor
    Baojun Wang
    Article
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    araC (araC )
    mCherryN(NGK).Cth-Ter*.(SEL)mCherryC.H6
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    P(araBAD) and iPC
    Available Since
    March 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    138234-plasmid-map-sequence-id-273460

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  30. Dox-Akt

    Plasmid
    #207328
    Purpose
    PiggyBac vector backbone encoding doxycycline-inducible expression of membrane-localized Akt1 (without pleckstrin homology domain)
    Depositor
    Jared Toettcher
    Article
    Underhill et al Development. 2023 Aug 15;150(16):dev2016
    Insert
    rac protein kinase-alpha (AKT1 Human)
    Tags
    myristoylation tag - BFP
    Expression
    Bacterial
    Mutation
    range: 129 - 480
    Promoter
    Tet Response Element
    Available Since
    Nov. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    207328-plasmid-map-sequence-id-413074

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  32. pCW-eGFP-SHLD2-m1

    Plasmid
    #114120
    Purpose
    Lentiviral vector for inducible expression of N-terminally tagged eGFP-SHLD2, m1 mutant
    Depositor
    Daniel Durocher
    Article
    Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:
    Insert
    SHLD2 (m1 mutant) (SHLD2 Human)
    Use
    Lentiviral; Doxycycline inducible
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    W489A, F494A, W495A mutations introduced
    Promoter
    TRE promoter, Tet ON
    Available Since
    Aug. 28, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    114120-plasmid-map-sequence-id-220066

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  33. pDB070.iodo.5

    Plasmid
    #99397
    Purpose
    Expression plasmid encoding the evolved MjTyrRS variant Iodo.5RS for amber suppression with p-iodophenylalanine
    Depositor
    David Liu
    Article
    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038
    Insert
    PN25 TetR; Ptet Iodo.5RS; PproK tyrT(Opt,CUA)
    Expression
    Bacterial
    Promoter
    PN25, Ptet, and PproK
    Available Since
    Nov. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    99397-plasmid-map-sequence-id-494133

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  34. pCW57.1 KBTBD4 R313PRR

    Plasmid
    #184626
    Purpose
    Doxycycline inducible lentiviral vector for N-terminal Flag tagged KBTBD4 R313PRR expression in mammalian cells
    Depositor
    Vincenzo D'Angiolella
    Article
    Chen et al Cell Death Differ. 2022 Apr 4. pii: 10.1
    Insert
    N-terminal Flag tagged KBTBD4 R313PRR (KBTBD4 Human)
    Use
    Lentiviral; Doxycycline inducible
    Expression
    Mammalian
    Mutation
    R313PRR
    Promoter
    TRE promoter, Tet ON
    Available Since
    June 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    184626-plasmid-map-sequence-id-363382

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  36. pFD115

    Plasmid
    #124768
    Purpose
    pFD115 carries cas9 controlled by an aTc-inducible Ptet promoter, a sgRNA controlled constitutively from PpflB S. aureus promoter to clone a guide between two BsaI sites and oriT on pLZ12 vector
    Depositor
    David Bikard
    Article
    Depardieu et al Methods. 2019 Aug 1. pii: S1046-2023(18)
    Inserts
    Ptet
    cas9
    term PpflB sgRNA (BsaI)
    Use
    CRISPR
    Promoter
    Ptet
    Available Since
    Aug. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    124768-plasmid-map-sequence-id-246185

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  38. MLM3743

    Plasmid
    #49962
    Purpose
    Expresses a mutant TALE-TET1CD (inactive catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene for use as a control
    Depositor
    Keith Joung
    Article
    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038
    Insert
    3x Flag Tet1CDmut HB-5 (HBB Human)
    Use
    TALEN
    Tags
    3x FLAG
    Mutation
    catalytic domain of TET1 inactive (H1671Y and D16…
    Promoter
    EF1a
    Available Since
    Dec. 31, 2013
    Availability
    Academic Institutions and Nonprofits only

  41. pAraH6HATT

    Plasmid
    #63901
    Purpose
    For expression of soluble scFv/Fab fragments in bacteria from genes selected using the pComb3H system, contains TT Fab (light and heavy chains) against tetanus toxin, used as expression control.
    Depositor
    Carlos Barbas
    Article
    Barbas et al Methods, A companion to Methods in Enzym
    Insert
    TT Fab
    Tags
    6xHis and HA tag
    Expression
    Bacterial
    Promoter
    araBAD
    Available Since
    Feb. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    63901-plasmid-map-sequence-id-136457

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Showing: 4251 - 4300 of 7002 results