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Synthetic Biology

12,047 results

Showing: 4351 - 4400 of 12047 results

  1. pPD294 ZF43x10-Compact_EYFP

    Plasmid
    #138905
    Purpose
    EYFP reporter vector for the ZF43x10-Compact promoter
    Depositor
    Joshua Leonard
    Insert
    ZF43x10-Compact
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    ZF43x10-Compact
    Available since
    Feb. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    138905-plasmid-map-sequence-id-324140

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  6. C86m

    Plasmid
    #120997
    Purpose
    MoClo golden gate assembly CD part (phage lysis protein; lethal to host when expressed). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    X174E
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120997-plasmid-map-sequence-id-236126

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  11. LSB-hsa-miR-302a-3p

    Plasmid
    #103400
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-302a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-302a-3p target (MIR302A Human)
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a
    Available since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103400-plasmid-map-sequence-id-195849

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  12. pUC19-Top-Toehold-mCherry

    Plasmid
    #87318
    Purpose
    Expresses the "Top" transcript of the Split-Broccoli system upstream of an RNA Toehold switch controlling mCherry.
    Depositor
    Donald Burke
    Article
    Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102
    Insert
    Split-Broccoli "Top" transcript upstream of an RNA Toehold (TS2_KS01) controlling mCherry translation.
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    P70a
    Available since
    May 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    87318-plasmid-map-sequence-id-174668

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  14. LSB-hsa-miR-885-3p

    Plasmid
    #103744
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-885-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-885-3p target (MIR885 Human)
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a
    Available since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103744-plasmid-map-sequence-id-196536

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  16. pMM2_12

    Plasmid
    #183061
    Purpose
    Control for biosensor in pMM2_9: BM3R1-HAA1-mTurquoise2 under HAA1p and mCherry under HREsYGP1p with flanks for genome integration into HO locus
    Depositor
    Yvonne Nygård
    Article
    Mormino et al FEMS Yeast Res. 2021 Sep 11;21(6). pii:
    Inserts
    mCherry
    BM3R1-HAA1-mTurquoise2
    Use
    Synthetic Biology
    Tags
    mCherry and mTurquoise2
    Expression
    Yeast
    Mutation
    Promoter
    HAA1 and Synthetic promoter
    Available since
    June 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    183061-plasmid-map-sequence-id-363441

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  17. pMM2_4

    Plasmid
    #183057
    Purpose
    HAA1-mTurquoise2 under HAA1p and mRuby2 under YGP1p with flanks for genome integration into HO locus
    Depositor
    Yvonne Nygård
    Article
    Mormino et al FEMS Yeast Res. 2021 Sep 11;21(6). pii:
    Inserts
    mRuby2
    HAA1-mTurquoise2
    Use
    Synthetic Biology
    Tags
    mRuby2 and mTurquoise2
    Expression
    Yeast
    Mutation
    Promoter
    HAA1 and YGP1
    Available since
    June 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    183057-plasmid-map-sequence-id-363440

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  18. pMM2_10

    Plasmid
    #183059
    Purpose
    Control for biosensor in pMM2_9: HAA1-mTurquoise2 under HAA1p and mCherry under HREsYGP1p with flanks for genome integration into HO locus
    Depositor
    Yvonne Nygård
    Article
    Mormino et al FEMS Yeast Res. 2021 Sep 11;21(6). pii:
    Inserts
    mCherry
    HAA1-mTurquoise2
    Use
    Synthetic Biology
    Tags
    mCherry and mTurquoise2
    Expression
    Yeast
    Mutation
    Promoter
    HAA1 and Synthetic promoter
    Available since
    June 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    183059-plasmid-map-sequence-id-363439

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  19. pMM2_9

    Plasmid
    #183058
    Purpose
    Biosensor for acetic acid: BM3R1-Haa1-mTurquoise2 under HAA1p and mCherry under BM3R1ENO1cp with flanks for genome integration in toHO locus
    Depositor
    Yvonne Nygård
    Article
    Mormino et al FEMS Yeast Res. 2021 Sep 11;21(6). pii:
    Inserts
    mCherry
    BM3R1-HAA1-mTurquoise2
    Use
    Synthetic Biology
    Tags
    mCherry and mTurquoise2
    Expression
    Yeast
    Mutation
    Promoter
    HAA1 and Synthetic promoter
    Available since
    June 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    183058-plasmid-map-sequence-id-363425

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  20. pMM2_11

    Plasmid
    #183060
    Purpose
    Control for biosensor in pMM2_9: HAA1-mTurquoise2 under HAA1p and mCherry under HREsENO1cp with flanks for genome integration into HO locus
    Depositor
    Yvonne Nygård
    Article
    Mormino et al FEMS Yeast Res. 2021 Sep 11;21(6). pii:
    Inserts
    mCherry
    HAA1-mTurquoise2
    Use
    Synthetic Biology
    Tags
    mCherry and mTurquoise2
    Expression
    Yeast
    Mutation
    Promoter
    HAA1 and Synthetic promoter
    Available since
    June 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    183060-plasmid-map-sequence-id-363416

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  21. deltaVP1 + VP2C-GFP

    Plasmid
    #166676
    Purpose
    Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and GFP tagged with VP2C (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3)
    Depositor
    Claudia Vickers
    Article
    Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-
    Inserts
    VP2C-GFP
    Murine polyomavirus deltaVP1 (NLS deletion mutant)
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1 and GAL10
    Available since
    April 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    166676-plasmid-map-sequence-id-329830

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  23. pJBL718

    Plasmid
    #140388
    Purpose
    Expresses MobR-regulated 3WJdB RNA aptamer driven by T7 promoter with the mobO sequence 5BP downstream from the promoter
    Depositor
    Julius Lucks
    Article
    Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038
    Insert
    T7-mobO-3WJdB-T
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    140388-plasmid-map-sequence-id-297945

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  26. pBlastR-5xUAS-mCherry-MiniWhite

    Plasmid
    #165911
    Purpose
    Blasticidin resistant 5xUAS mCherry Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening
    Depositor
    Koen Venken
    Article
    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi
    Insert
    Selectable UAS Response Vector
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    165911-plasmid-map-sequence-id-326310

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  27. pPuroR-MCS-GAL4-MiniWhite

    Plasmid
    #165890
    Purpose
    Puromycin resistant GAL4 driver vector with Mini-w+ CDS eye marker. Enhancer grammar GB20 entry point for custom enhancers. Cut-and-paste cloning can be used. Purple-white bacteria colony screening.
    Depositor
    Koen Venken
    Article
    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi
    Insert
    Selectable Empty Enhancer Driver
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    165890-plasmid-map-sequence-id-329406

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  28. pG418R-MCS-QF-MiniWhite

    Plasmid
    #165902
    Purpose
    G418 resistant QF driver vector with Mini-white CDS eye marker. Contains an enhancer grammar GB20 entry point for custom enhancers. Standard cut-and-paste cloning can also be used. Vector uses Purple-White bacteria colony screening for identification of correct cloning
    Depositor
    Koen Venken
    Article
    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi
    Insert
    Selectable Empty Enhancer Driver
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    165902-plasmid-map-sequence-id-328281

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  31. DT050A

    Plasmid
    #159411
    Purpose
    Constitutive murine mature BDNF expression, controlled under E.coli J23101 (strong) promoter
    Depositor
    Sheref Mansy
    Article
    Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea
    Insert
    pr.J23101-BDNF (Bdnf, Mouse) (Bdnf Mouse, Synthetic)
    Use
    Synthetic Biology
    Tags
    FLAG
    Expression
    Bacterial
    Mutation
    Promoter
    J23101 promoter
    Available since
    March 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    159411-plasmid-map-sequence-id-326574

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  34. KZ501: pMVP (L3-L2) P2A-Puro + polyA

    Plasmid
    #121780
    Purpose
    pMVP L3-L2 entry plasmid, contains Puro-P2A + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term Puro selection marker linked by P2A to gene of interest.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    P2A-Puro + polyA
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121780-plasmid-map-sequence-id-237276

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  35. LSB-hsa-miR-579-5p

    Plasmid
    #103661
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-579-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-579-5p target (MIR579 Human)
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a
    Available since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103661-plasmid-map-sequence-id-196370

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  37. pFAB1935

    Plasmid
    #47860
    Purpose
    BIOFAB reporter plasmid for measuring his_T_min_linker_crp_T_min termination efficiency
    Depositor
    Drew Endy
    Article
    Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:
    Insert
    his_T_min_linker_crp_T_min double terminator
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    concatenate of his_min and crp_min
    Promoter
    pLTetO15
    Available since
    Dec. 3, 2013
    Availability
    Academic Institutions and Nonprofits only
    47860-plasmid-map-sequence-id-68392

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  38. P9m

    Plasmid
    #120937
    Purpose
    MoClo golden gate assembly AB part for pOR1-OR2 (lambda cI-repressable strong promoter) - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    Pr-OR1-OR2
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120937-plasmid-map-sequence-id-247706

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  41. LSB-hsa-miR-302c-3p

    Plasmid
    #103403
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-302c-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-302c-3p target (MIR302C Human)
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a
    Available since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103403-plasmid-map-sequence-id-195855

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  42. pdCas9-M-3F2

    Plasmid
    #73428
    Purpose
    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 3F2.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Repressor C4 (orthogonal T7-lac repressor)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Constitutive wild-type S. pyogenes promoter
    Available since
    April 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    73428-plasmid-map-sequence-id-135328

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  44. LSB-hsa-miR-519c-3p

    Plasmid
    #103608
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-519c-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-519c-3p target (MIR519C Human)
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a
    Available since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103608-plasmid-map-sequence-id-196264

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  45. LSB-hsa-miR-520c-3p

    Plasmid
    #103610
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-520c-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-520c-3p target (MIR520C Human)
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a
    Available since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103610-plasmid-map-sequence-id-196268

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  48. pNtCp-rbcL-accD

    Plasmid
    #52469
    Purpose
    Contains rbcL and accD DNA regions for double homologous recombination useful in Nicotiana tabacum Cp transformation
    Depositor
    Anastasios Melis
    Insert
    rbcL-accd Nicotiana tabacum Cp DNA
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    52469-plasmid-map-sequence-id-81328

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Showing: 4351 - 4400 of 12047 results