4,124 results

1. pENTR1A-GFP-N2 (FR1)

   Plasmid

   #19364

   Purpose

   Gateway entry vector for C-term eGFP fusion, frame N2

   Depositor

   Paul Kaufman, Eric Campeau

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Insert

   Enhanced Green Fluorescent Protein
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHAGE-TO-nmdCas9-3XmCherry

   Plasmid

   #64110

   Purpose

   Expression of Nm dCas9-3XmCherry in mammalian cells

   Depositor

   Thoru Pederson

   Article

   Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

   Insert

   Nm dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   3XmCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-TO

   Available since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV5 TBRI-His

   Plasmid

   #19161

   Depositor

   Joan Massague

   Insert

   transforming growth factor, beta receptor 1 (TGFBR1 Human)
   

   Use

   Tags

   His

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 1, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3 236HSC WT (BRCA2)

   Plasmid

   #16246

   Depositor

   Mien-Chie Hung

   Article

   Wang et al Cancer Res. 2002 Mar 1. 62(5):1311-4.

   Insert

   BRCA2 (BRCA2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 30, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSBFP2-C1

   Plasmid

   #22880

   Depositor

   Dorus Gadella

   Article

   Kremers et al Biochemistry. 2007 Mar 27. 46(12):3775-8

   Insert

   SBFP2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 24, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. Reduced Expression GFP beta actin

   Plasmid

   #31502

   Depositor

   Rick Horwitz, Tim Mitchison

   Article

   Watanabe et al Science. 2002 Feb 8. 295(5557):1083-6.

   Insert

   beta actin (ACTB Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   91-544 of enhancer region of CMV promoter deleted

   Promoter

   Available since

   Aug. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pL-CRISPR.EFS.tRFP

   Plasmid

   #57819

   Purpose

   Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses tagRFP via P2A cleavage site. EFS Promoter driven

   Depositor

   Benjamin Ebert

   Article

   Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

   Inserts

   SpCas9
   Sp sgRNA scaffold
   EFS (EEF1A1 Human)
   P2A-tRFP
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   Available since

   Aug. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-6xHis-(pos36)GFP

   Plasmid

   #62937

   Purpose

   Expression of 6xHis-(+36)GFP in bacterial cells

   Depositor

   David Liu

   Article

   Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

   Insert

   (pos36)GFP
   

   Use

   Tags

   6xHis tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. SP-Cas9

   Plasmid

   #62731

   Purpose

   Expresses SP-Cas9 protein in bacterial cells

   Depositor

   Niels Geijsen

   Article

   D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

   Insert

   SP-CAS9
   

   Use

   CRISPR

   Tags

   His tag and NLS

   Expression

   Bacterial

   Mutation

   Codon optimazed for e.coli

   Promoter

   T7

   Available since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCALNL-GFP

    Plasmid

    #13770

    Purpose

    Cre recombinase-dependent expression of GFP, i.e. GFP is only expressed in the presence of Cre due to a transcriptional stop in between the loxP sites.

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

    Insert

    EGFP
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 20, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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11. pR26 CAG/GFP Dest

    Plasmid

    #74281

    Purpose

    Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter, loxP flanked stop cassette, GFP, for gateway cloning

    Depositor

    Ralf Kuehn

    Article

    Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

    Insert

    Rosa26 5-homology region
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDNA3-HA-HA-humanCMYC

    Plasmid

    #74164

    Purpose

    Mammalian expression of human cMyc

    Depositor

    Martine Roussel

    Article

    Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

    Insert

    cMyc (MYC Human)
    

    Use

    Tags

    2x HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMXs-hc-MYC

    Plasmid

    #17220

    Purpose

    Retroviral expression of human cMYC

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al Cell. 2007 Nov 30. 131(5):861-72.

    Insert

    c-MYC (MYC Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Feb. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pICE-RNaseHI-D10R-E48R-NLS-mCherry

    Plasmid

    #60367

    Purpose

    Plasmid for expression of an inactive mutant of bacterial RNase HI tagged with NLS-mCherry that can be used to detect R-loops. Confers resistance to Puromycin. Expression is inducible in T-REx cells.

    Depositor

    Patrick Calsou, Sébastien Britton

    Article

    Britton et al Nucleic Acids Res. 2014 Oct 1;42(14):904

    Insert

    RNase HI-D10R-E48R
    

    Use

    Tags

    NLS from SV40 T antigen and mCherry

    Expression

    Mammalian

    Mutation

    D10R+E48R; catalyticaly inactive

    Promoter

    CMV-tet

    Available since

    Jan. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. DsRed-rab11 WT

    Plasmid

    #12679

    Depositor

    Richard Pagano

    Article

    Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

    Insert

    RAB11A (Homo sapiens) (RAB11A Human)
    

    Use

    Tags

    DsRed and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. mCitrine-N1

    Plasmid

    #54594

    Purpose

    Localization: N1 Cloning Vector, Excitation: 516, Emission: 529

    Depositor

    Roger Tsien, Robert Campbell, Michael Davidson, Oliver Griesbeck

    Article

    Griesbeck et al J Biol Chem. 2001 Aug 3;276(31):29188-94

    Type

    Empty backbone

    Use

    Tags

    mCitrine

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. PSD-95 FLAG

    Plasmid

    #15463

    Depositor

    Wei-dong Yao

    Article

    Zhang et al J Biol Chem. 2007 May 25. 282(21):15778-

    Insert

    PSD-95 (Dlg4 Rat)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 25, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRK5 alpha6

    Plasmid

    #16036

    Depositor

    Filippo Giancotti

    Article

    Dans et al J Biol Chem. 2001 Jan 12. 276(2):1494-50

    Insert

    alpha6 (ITGA6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHIV-Luciferase

    Plasmid

    #21375

    Purpose

    self-inactivating 3rd generation lentiviral plasmid for co-expression of your gene of interest and Luciferase

    Depositor

    Bryan Welm

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 19, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PB-TA-ERP2

    Plasmid

    #80477

    Purpose

    All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (constitutive rtTA and puromycin resistance)

    Depositor

    Knut Woltjen

    Article

    Kim et al Methods Mol Biol. 2016;1357:111-31. doi:

    Type

    Empty backbone

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCAG mito-GCaMP5G

    Plasmid

    #105009

    Purpose

    mitochondrial calcium sensor

    Depositor

    Franck Polleux

    Article

    Kwon et al PLoS Biol. 2016 Jul 18;14(7):e1002516. d

    Insert

    GCaMP5G
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA3-TORCAR(T/A)

    Plasmid

    #64928

    Purpose

    Negative-control mutant for TORCAR biosensor.

    Depositor

    Jin Zhang

    Article

    Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

    Insert

    TORCAR(T/A) (EIF4EBP1 Human, Synthetic)
    

    Use

    Tags

    Cerulean and YPet

    Expression

    Mammalian

    Mutation

    4EBP1 domain contains T37A and T46A mutations.

    Promoter

    CMV

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. GFP-ERK1

    Plasmid

    #14747

    Depositor

    Rony Seger

    Article

    Yung et al J Biol Chem. 2001 Sep 21. 276(38):35280-

    Insert

    ERK1 (MAPK3 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Stat1 alpha Flag pRc/CMV

    Plasmid

    #8691

    Depositor

    Jim Darnell

    Article

    Horvath et al Genes Dev 1995 Apr 15;9(8):984-94.

    Insert

    STAT1 alpha (STAT1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pFR_CrPV_xb

    Plasmid

    #11509

    Depositor

    Phil Sharp

    Article

    Petersen et al Mol Cell. 2006 Feb 17. 21(4):533-42.

    Insert

    Firefly luciferase, Cricket Paralysis Virus IRES sequence, Renilla, and 3'UTR short RNA binding sites
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. FH-TET3-pEF

    Plasmid

    #49446

    Purpose

    This plasmid expresses full-length human TET3.

    Depositor

    Anjana Rao

    Article

    Ko et al Nature. 2013 May 2;497(7447):122-6. doi:

    Insert

    Tet methylcytosine dioxygenase 3 (TET3 Human)
    

    Use

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1alpha

    Available since

    Jan. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMKO.1 puro

    Plasmid

    #8452

    Purpose

    Retroviral vector for shRNA expression.

    Depositor

    Bob Weinberg

    Article

    Stewart et al RNA 2003 Apr;9(4):493-501.

    Type

    Empty backbone

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. GFP-hPIKfyve

    Plasmid

    #121148

    Purpose

    Expresses GFP-tagged human PIKfyve in mammalian cells.

    Depositor

    Geert van den Bogaart

    Article

    Baranov et al iScience. 2019 Jan 25;11:160-177. doi: 1

    Insert

    1-phosphatidylinositol 3-phosphate 5-kinase isoform 2 (PIKFYVE Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAnc80L65AAP

    Plasmid

    #92307

    Purpose

    Adeno-associated Viral Vector (AAV) capsid Anc80L65 in AAV2Rep expression construct with endogenous AAP

    Depositor

    Luk Vandenberghe

    Article

    Landegger et al Nat Biotechnol. 2017 Mar;35(3):280-284.

    Insert

    Ancestral AAV Capsid Anc80L65AAP
    

    Use

    AAV and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Rep2

    Available since

    Aug. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. GLUT1-eGFP/pcDNA-DEST47

    Plasmid

    #18729

    Depositor

    Wolf Frommer

    Article

    Takanaga et al Biochim Biophys Acta. 2008 Apr . 1778(4)

    Insert

    pcDNA-DEST47 /hGLUT1-eGFP
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    deleted TAA

    Promoter

    Available since

    July 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge