20,196 results

Showing: 3521 - 3560 of 20196 results

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pET-28c S2

Plasmid

#171920

Purpose

Express RNA-based (p)ppGpp sensor

Depositor

Mingxu You

Article

Sun et al Angew Chem Int Ed Engl. 2021 Nov 2;60(45

Insert

RNA-based (p)ppGpp sensor

Expression

Bacterial

Promoter

T7

Available Since

July 15, 2021

Availability

Academic Institutions and Nonprofits only

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pZac2.1-GfaABC1D-Rpl22-HA

Plasmid

#111811

Purpose

Express Rpl22HA in astrocytes

Depositor

Baljit Khakh

Article

Yu et al Neuron. 2018 Aug 27. pii: S0896-6273(18)

Insert

Ribosomal protein L22 (Rpl22 Mouse)

Tags

HA

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-Mm.cargo(Cas9)

Plasmid

#174865

Purpose

Expresses mouse Peg10 cargoRNA encoding SpCas9

Depositor

Feng Zhang

Article

Segel et al Science. 2021 Aug 20;373(6557):882-889.

Insert

SpCas9 flanked by MmPeg10 UTRs

Tags

HA-SV40 NLS and SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pY016 (pcDNA3.1-hLbCpf1)

Plasmid

#69988

Purpose

Expresses humanized LbCpf1

Depositor

Feng Zhang

Article

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

hLbCpf1

Use

CRISPR

Tags

NLS-3xHA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

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phDLX1-N174

Plasmid

#60859

Purpose

2nd generation lentiviral transfer plasmid. Expresses DLX1 under the EF1a promoter

Depositor

Andrew Yoo

Article

Victor et al Neuron. 2014 Oct 22;84(2):311-23. doi: 1

Insert

DLX1 (DLX1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pMIG-FLAG-MLL-AF9

Plasmid

#71443

Purpose

Retroviral construct to express FLAG-tagged MLL-AF9 fusion gene, followed by IRES-GFP

Depositor

Daisuke Nakada

Article

Saito et al Cell Stem Cell. 2015 Sep 29. pii: S1934-

Insert

MLL-Af9 (KMT2A Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Promoter

MSCV

Available Since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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MSCV-Myc-IRES-GFP

Plasmid

#35396

Depositor

Martine Roussel

Article

Kawauchi et al Cancer Cell. 2012 Feb 14;21(2):168-80.

Inserts

Myc (Myc Mouse)
GFP

Use

Retroviral

Expression

Mammalian

Available Since

March 16, 2012

Availability

Academic Institutions and Nonprofits only
pSG5-BZLF1

Plasmid

#72637

Purpose

Mammalian expression vector for Epstein-Barr virus Zta immediate-early transcriptional regulator

Depositor

S. Diane Hayward

Article

Sarisky et al J Virol. 1996 Dec;70(12):8340-7.

Insert

BZLF1

Expression

Mammalian

Mutation

Q163R

Available Since

Feb. 11, 2016

Availability

Academic Institutions and Nonprofits only
pET28b-hUba2-His

Plasmid

#53117

Purpose

expression of full length human Uba2 with Cterminal His-tag in E.coli

Depositor

Frauke Melchior

Article

Werner et al Methods Mol Biol. 2009;497:187-99. doi:

Insert

UBA2 (UBA2 Human)

Tags

His and T7 tag

Expression

Bacterial

Promoter

T7

Available Since

Dec. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pDONR223-FGFR3

Plasmid

#23933

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

FGFR3 (FGFR3 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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YFP-Parkin

Plasmid

#23955

Purpose

Mammalian expression of human Park2 fused to EYFP

Depositor

Richard Youle

Article

Narendra et al J Cell Biol. 2008 Dec 1. 183(5):795-803.

Insert

Park2 (PRKN Human)

Tags

eYFP

Expression

Mammalian

Available Since

April 9, 2010

Availability

Academic Institutions and Nonprofits only

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Galphai1-mTurquoise2

Plasmid

#69620

Purpose

The heterotrimeric G-protein subunit, Gαi isoform 1, tagged with mTurquoise2

Depositor

Dorus Gadella

Article

van Unen et al PLoS One. 2016 Jan 22;11(1):e0146789. do

Insert

Galphai1-mTurquoise2

Tags

mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only
pCDNA3.1-FLAG-SF3B1-WT

Plasmid

#82576

Purpose

Expresses a codon-optimized ORF of human SF3B1 (wild-type)

Depositor

Manoj Pillai

Article

Kesarwani et al Oncogene. 2016 Aug 15. doi: 10.1038/onc.

Insert

human SF3B1-WT (SF3B1 Human)

Tags

FLAG peptide

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR223_ARNTL_WT

Plasmid

#82189

Purpose

Gateway Donor vector containing ARNTL , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

ARNTL (BMAL1 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221_SV2A

Plasmid

#132257

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SV2A (SV2A Human)

Expression

Mammalian

Available Since

Nov. 21, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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MSCV-human-c-MYC-ER-mouse IRES-mcherry

Plasmid

#73570

Purpose

Mammalian retroviral expression of Myc-ER

Depositor

Martine Roussel

Article

Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

Insert

cMYC (MYC Human)

Use

Retroviral

Tags

IRES-mCherry and Mouse ER

Expression

Mammalian

Promoter

MSCV

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only
pMRXIP GFP-Stx17 WT

Plasmid

#45909

Depositor

Noboru Mizushima

Article

Itakura et al Cell. 2012 Dec 7;151(6):1256-69. doi: 10

Insert

Syntaxin 17 (STX17 Human)

Use

Retroviral

Tags

GFP

Promoter

CMV

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pAnc80L65AAP

Plasmid

#92307

Purpose

Adeno-associated Viral Vector (AAV) capsid Anc80L65 in AAV2Rep expression construct with endogenous AAP

Depositor

Luk Vandenberghe

Article

Landegger et al Nat Biotechnol. 2017 Mar;35(3):280-284.

Insert

Ancestral AAV Capsid Anc80L65AAP

Use

AAV and Synthetic Biology

Expression

Mammalian

Promoter

Rep2

Available Since

Aug. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pJL1-dTomato

Plasmid

#102631

Purpose

In vitro expression of tdTomato from the T7 promoter

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

Insert

tdTomato

Expression

Bacterial

Promoter

T7

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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phCMV-229E-D15

Plasmid

#188908

Purpose

express truncated 229E spike for pseudovirus production

Depositor

David Nemazee

Insert

229E spike (S 229E)

Expression

Mammalian

Mutation

c-terminal 15aa deletion

Promoter

CMV

Available Since

Aug. 30, 2022

Availability

Academic Institutions and Nonprofits only

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HDM-Hgpm2

Plasmid

#204152

Purpose

Lentivirus helper plasmid coding for Gag/Pol

Depositor

Jesse Bloom

Article

Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

Insert

HIV-1 gag/pol

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.CAG-FLEX.SF-iGluSnFR.A184S

Plasmid

#106186

Purpose

Tight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-iGluSnFR.A184S

Use

AAV

Mutation

GltI: A184S

Promoter

CAG

Available Since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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pBAD/HisB-iRFP720

Plasmid

#45460

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

Insert

iRFP720

Expression

Bacterial

Mutation

T2A, S13L, A92T, V104T, V114I, E161K, E180S, Y193…

Promoter

araBAD

Available Since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3-CXCR4

Plasmid

#98942

Purpose

Mammalian expression plasmid for untagged human CXCR4

Depositor

Erik Procko

Article

Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

Insert

CXCR4 (CXCR4 Human)

Expression

Mammalian

Mutation

Full-length, wildtype

Promoter

CMV

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223_CLOCK_WT

Plasmid

#82247

Purpose

Gateway Donor vector containing CLOCK , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CLOCK (CLOCK Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
HDM-tat1b

Plasmid

#204154

Purpose

Lentivirus helper plasmid coding for Tat

Depositor

Jesse Bloom

Article

Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

Insert

tat

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
MSGV-1D3-28Z All ITAMs intact

Plasmid

#107226

Purpose

Anti-mouse CD19 CAR with CD3 ITAMs intact

Depositor

James Kochenderfer, Steven Rosenberg

Article

Kochenderfer et al Blood. 2010 Nov 11;116(19):3875-86. doi:

Insert

1D3-28Z All ITAMs intact

Use

Retroviral

Tags

None

Mutation

None

Available Since

March 30, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pTriEx-NTOM20-mVenus-Zdk2

Plasmid

#81011

Purpose

Expression of Zdk2 anchored to mitochondria

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

Zdk2

Tags

6x His tag, FLAG, NTOM20, and mVenus

Expression

Bacterial, Insect, and Mamm…

Available Since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
PMXS-NDI1

Plasmid

#72876

Purpose

expresses NDI1 in mammalian cells

Depositor

David Sabatini

Article

Birsoy et al Nature. 2014 Apr 3;508(7494):108-12. doi

Insert

NDI1 (NDI1 Budding Yeast)

Use

Retroviral

Expression

Mammalian

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCW57.1_V5_TurboID_FLAG_Ct

Plasmid

#194073

Purpose

3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-blasti

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Type

Empty backbone

Use

Lentiviral

Tags

FLAG-TurboID-V5

Expression

Mammalian

Available Since

Jan. 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pC013 - Twinstrep-SUMO-huLwCas13a

Plasmid

#90097

Purpose

Twinstrep-SUMO-huLwCas13a for recombinant protein bacterial expression. Insert is human codon optimized but expresses well in bacteria.

Depositor

Feng Zhang

Article

Gootenberg et al Science. 2017 Apr 13. pii: eaam9321. doi

Insert

LwCas13a

Use

CRISPR

Tags

6xHis-Twin Strep-SUMO

Expression

Bacterial

Available Since

June 13, 2017

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pBS_12x-601_MA1

Plasmid

#114358

Purpose

12x Widom 601 nucleosome sequence

Depositor

Scot Wolfe

Article

Swygert et al Proc Natl Acad Sci U S A. 2018 Dec 4;115

Insert

12X601-MA1

Use

Unspecified

Expression

Mammalian

Available Since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pFH2.105_PB-EPN24-MCP-T2A-eGFP-PGK-Blast

Plasmid

#205550

Purpose

Express EPN24-MCP-T2A-eGFP and blasticidin resistance marker

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

EPN24-MCP

Use

Piggybac

Expression

Mammalian

Mutation

WT

Promoter

EF1

Available Since

Sept. 19, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
MAC_C_CH60

Plasmid

#111662

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Insert

CH60 (HSPD1 Human)

Expression

Mammalian

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-ADAM10-HA

Plasmid

#65106

Purpose

Expression of HA tagged ADAM10 in mammalian cells

Depositor

Axel Ullrich

Article

Gschwind et al EMBO J. 2003 May 15;22(10):2411-21.

Insert

ADAM10 (ADAM10 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 25, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pBAD-HisD-miniGFP1

Plasmid

#188896

Purpose

Express 6xHis-Tag miniGFP1 in E.coli

Depositor

Kiryl Piatkevich

Article

Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

Insert

miniGFP1

Tags

6xHis

Expression

Bacterial

Promoter

araBAD

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CAG-iGluSnFR4s-NGR-WPRE

Plasmid

#234451

Purpose

AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.

Depositor

GENIE Project

Article

Aggarwal et al Nat Methods. 2026 Feb;23(2):417-425. doi

Insert

iGluSnFR4s-NGR

Use

AAV

Tags

IgK chain and Myc epi tag-NGR GPI anchor

Expression

Mammalian

Promoter

CAG

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
FLAG-HA-pcDNA3.1-

Plasmid

#52535

Purpose

enables eukaryotic expression of N-terminally FLAG-HA-tagged fusion proteins

Depositor

Adam Antebi

Article

Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

Has Service

Cloning Grade DNA

Type

Empty backbone

Tags

FLAG-HA

Expression

Mammalian

Promoter

CMV

Available Since

July 31, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV5 MEKK3 WT

Plasmid

#12186

Depositor

Gary Johnson

Article

Blank et al J Biol Chem. 1996 Mar 8. 271(10):5361-8.

Insert

MEKK3 (Map3k3 Mouse)

Tags

HA

Expression

Mammalian

Available Since

Sept. 22, 2006

Availability

Academic Institutions and Nonprofits only
S1PR3-Tango

Plasmid

#66498

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

S1PR3 (S1PR3 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only

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20,196 results