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Addgene

We narrowed to 34,443 results for: CaS

Showing: 4401 - 4450 of 34443 results
  1. pSLIK CA ROCK2

    Plasmid
    #84649
    Purpose
    Lentiviral expression of doxycycline-inducible constitutively active ROCK2 and co-expression of Venus
    Depositor
    Insert
    ROCK2 (ROCK2 Bovine)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Constitutive Active (please see depositor comment…
    Promoter
    Available since
    Dec. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
  2. pAAV-STAT-CA

    Plasmid
    #195576
    Purpose
    Expresses STAT3 constitutively active mutant
    Depositor
    Insert
    Signal transducer and activator of transcription 3 (Stat3 Mouse)
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    changed Alanine 662 to Cysteine and Asparagine 66…
    Promoter
    CMV enhancer and promoter
    Available since
    Feb. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
  3. SG3ΔENV CA E45A

    Plasmid
    #145783
    Purpose
    Non-infectious HIV-1 Clone with stop codon in Env (see NIH AIDS Reagent Program Cat #11051) and E45A mutation in capsid (CA E45A).
    Depositor
    Insert
    Capsid
    Use
    Lentiviral and Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Changed Capsid residue glutamic acid 45 to alanin…
    Promoter
    Available since
    Oct. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
  4. PB-CA tdTomato

    Plasmid
    #159095
    Purpose
    PB-CA tdTomato piggyBac transposon
    Depositor
    Insert
    tdTomato
    Use
    Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
  5. pENTR11 CA NFAT2

    Plasmid
    #11793
    Depositor
    Insert
    NFAT2 (Nfatc1 Mouse)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Constitutively active: multiple serine to alanine…
    Promoter
    Available since
    Aug. 7, 2006
    Availability
    Academic Institutions and Nonprofits only
  6. SG3ΔENV CA M66I

    Plasmid
    #149687
    Purpose
    Non-infectious HIV-1 Clone with stop codon in Env (see NIH AIDS Reagent Program Cat #11051) and M66I mutation in capsid (CA M66I).
    Depositor
    Insert
    Capsid
    Use
    Lentiviral and Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Changed Capsid residue Met 66 to Iso (CA M66I)
    Promoter
    Available since
    Oct. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
  7. pBad-CA TAG126

    Plasmid
    #105836
    Purpose
    Expresses thermostable variant of human carbonic anhydrase II with TAG stop codon at site 126 and C-terminal 6x his tag
    Depositor
    Article
    Insert
    human carbonic anhydrase II thermostable variant (CA2 Human)
    Use
    Tags
    6x his tag
    Expression
    Bacterial
    Mutation
    TAG stop codon at site 126
    Promoter
    pBad/arabinose
    Available since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
  8. pBad-CA TAG20

    Plasmid
    #105666
    Purpose
    expresses thermostable variant of human carbonic anhydrase II with TAG stop codon at site 20 and C-terminal 6x his tag
    Depositor
    Article
    Insert
    human carbonic anhydrase II thermostable variant (CA2 Human)
    Use
    Tags
    6x his tag
    Expression
    Bacterial
    Mutation
    TAG stop codon at site 20
    Promoter
    pBad/arabinose
    Available since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
  9. pBad-CA TAG93

    Plasmid
    #105667
    Purpose
    expresses thermostable variant of human carbonic anhydrase II with TAG stop codon at site 93 and C-terminal 6x his tag
    Depositor
    Article
    Insert
    human carbonic anhydrase II thermostable variant (CA2 Human)
    Use
    Tags
    6x his tag
    Expression
    Bacterial
    Mutation
    TAG stop codon at site 93
    Promoter
    pBad/arabinose
    Available since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
  10. PB-CA retrofitted

    Plasmid
    #159093
    Purpose
    piggyBac ITR's from PB-CA retrofitted with a linker
    Depositor
    Type
    Empty backbone
    Use
    Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
  11. pBad-CA TAG233

    Plasmid
    #105838
    Purpose
    Expresses thermostable variant of human carbonic anhydrase II with TAG stop codon at site 233 and C-terminal 6x his tag
    Depositor
    Article
    Insert
    human carbonic anhydrase II thermostable variant (CA2 Human)
    Use
    Tags
    6x his tag
    Expression
    Bacterial
    Mutation
    TAG stop codon at site 233
    Promoter
    pBad/arabinose
    Available since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
  12. pBad-CA TAG186

    Plasmid
    #105837
    Purpose
    Expresses thermostable variant of human carbonic anhydrase II with TAG stop codon at site 186 and C-terminal 6x his tag
    Depositor
    Article
    Insert
    human carbonic anhydrase II thermostable variant (CA2 Human)
    Use
    Tags
    6x his tag
    Expression
    Bacterial
    Mutation
    TAG stop codon at site 186
    Promoter
    pBad/arabinose
    Available since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
  13. 35S:YFPc-RabG3b-CA

    Plasmid
    #102372
    Purpose
    fluorescent tagging. Plant expression of YFPc-RabG3b-CA
    Depositor
    Insert
    RabG3B (RABG3B Mustard Weed)
    Use
    Tags
    YFPc
    Expression
    Plant
    Mutation
    Q67L
    Promoter
    35S
    Available since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
  14. IGI-P0492 pHR-dCas9-NLS-VPR-mCherry

    Plasmid
    #102245
    Purpose
    Lentiviral expression of dCas9-VPR-mCherry fusion protein for CRISPRa.
    Depositor
    Insert
    dCas9-VPR-mCherry
    Use
    CRISPR and Lentiviral
    Tags
    NLS-VPR-mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Oct. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
  15. pC0049-EF1a-dPSPCas13b-NES-HIV, H133A/H1058A

    Plasmid
    #103865
    Purpose
    Catalytically inactive PSPCas13b. RNase activity is inactivated by mutation of catalytic histidine residues in the HEPN domains (H133A/H1058A).
    Depositor
    Insert
    PspCas13b
    Use
    CRISPR and Lentiviral
    Tags
    3xHA and HIV NES
    Expression
    Mammalian
    Mutation
    Contains the H133A and H1058A mutations which ina…
    Promoter
    EF1a
    Available since
    Jan. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
  16. pCMV-T7-ABE8e-nSpCas9-VRQR-P2A-EGFP (KAC1309)

    Plasmid
    #185913
    Purpose
    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9-VRQR(D10A/D1135V/G1218R/R1335Q/T1337R) and P2A-EGFP
    Insert
    human codon optimized ABE8e-nSpCas9-VRQR-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9-VRQR=D10A/D1135V/G1218R/R1335Q/T1337R
    Promoter
    CMV and T7
    Available since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
  17. 783-Rx-hU6: RfxCas13d gRNA and array cloning backbone

    Plasmid
    #228360
    Purpose
    hU6-driven expression of RfxCas13d gRNAs and arrays compatible. Contains AarI sites for guide cloning flanked by 5' DR36
    Depositor
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral; Rfxcas13d grna expression …
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hU6
    Available since
    Feb. 5, 2025
    Availability
    Academic Institutions and Nonprofits only
  18. pCBh_NLS_dCas13X_NLS-pA-U6-DR-BpiI-BpiI-DR-pSV40-EGFP-pA-pSV40-mCherry-pA

    Plasmid
    #191796
    Purpose
    vector for encoding a human codon-optimized dead Cas13X (dCas13X) driven by CBh promoter, guide RNAs compatible with Cas13X driven by hU6, EGFP driven by SV40 promoter, and mCherry driven by SV40 promoter
    Depositor
    Insert
    humanized dCas13X
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    R84A, H89A, R739A, R740A, H745A, H746A, H747A
    Promoter
    CBh, SV40, hU6
    Available since
    Oct. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
  19. pX458-Ef1a-dCas9-VPR-H2B-GFP (CRISPRa)

    Plasmid
    #175571
    Purpose
    Inactivated Cas9 (dCas9) fusion to VPR for the purpose of targeted activation
    Depositor
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
Showing: 4401 - 4450 of 34443 results