Addgene

We narrowed to 1,018 results for: FIB

Showing: 401 - 450 of 1018 results

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human-GFAP-EGFP

Plasmid

#169473

Purpose

We generated a human-GFAP-EGFP fusion construct by randomly inserting the coding region of EFGP into the coding region of hGFAP.

Depositor

Federico Herrera

Article

Letra-Vilela et al Experimental Results (2020), Volume 1, e

Insert

EGFP-hGFAP (GFAP Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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pMXs-Runx1

Plasmid

#131609

Purpose

expression of mouse Runx1 in mammalian cells

Depositor

Kateri Moore, Filipe Pereira

Article

Pereira et al Cell Stem Cell. 2013 Aug 1;13(2):205-18.

Insert

Runx1 (Runx1 Mouse)

Use

Retroviral

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pTight-9-124

Plasmid

#31874

Depositor

Jerry Crabtree

Article

Yoo et al Nature. 2011 Jul 13. doi: 10.1038/nature

Insert

miR-9/9* and miR-124 (Mir124a-2, Mir9-3 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 30, 2011

Availability

Academic Institutions and Nonprofits only

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pMXs-Lhx3

Plasmid

#32927

Depositor

Kevin Eggan

Article

Son et al Cell Stem Cell. 2011 Sep 2;9(3):205-18.

Insert

Lhx3 (Lhx3 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

5'LTR

Available since

Nov. 30, 2011

Availability

Academic Institutions and Nonprofits only
pmCherry-C1_SEPT9 i1

Plasmid

#71622

Purpose

mammalian expression of human SEPT9 with an mCherry fusion

Depositor

Elias Spiliotis

Article

Dolat et al J Cell Biol. 2014 Oct 27;207(2):225-35.

Insert

Septin 9 (SEPTIN9 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pVenus-N-SLC35B2

Plasmid

#155038

Purpose

Transient expression of SLC35B2-Venus

Depositor

Yihong Ye

Article

Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

Insert

SLC35B2 (SLC35B2 Human)

Use

Tags

Venus

Expression

Mammalian

Mutation

See depositor comments below

Promoter

CMV

Available since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pMXs-Ngn2

Plasmid

#32928

Purpose

Retroviral expression of mouse Ngn2

Depositor

Kevin Eggan

Article

Son et al Cell Stem Cell. 2011 Sep 2;9(3):205-18.

Insert

Ngn2 (Neurog2 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

5'LTR

Available since

Nov. 30, 2011

Availability

Academic Institutions and Nonprofits only
pMXs-Klf4-IP

Plasmid

#15920

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

Kruppel-like factor 4 (gut) (Klf4 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

None

Promoter

Available since

Feb. 8, 2008

Availability

Academic Institutions and Nonprofits only

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pFUW-tetO-Relb

Plasmid

#139810

Purpose

doxycycline-inducible expression of mouse Relb in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

Insert

Relb (Relb Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE-mCMV

Available since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pMXs-Isl1

Plasmid

#32929

Depositor

Kevin Eggan

Article

Son et al Cell Stem Cell. 2011 Sep 2;9(3):205-18.

Insert

Isl1 (Isl1 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

5'LTR

Available since

Nov. 30, 2011

Availability

Academic Institutions and Nonprofits only
pLVX-Tight-Puro_Nkx2.2

Plasmid

#64840

Purpose

A lentiviral vector for the expression of mouse Nkx2.2 under doxycyline control.

Depositor

Paul Tesar

Article

Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

Insert

Nkx2.2 (Nkx2-2 Mouse)

Use

Lentiviral

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

May 28, 2015

Availability

Academic Institutions and Nonprofits only

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phMYT1L-N106

Plasmid

#60861

Purpose

Expresses MYT1L under the EF1a promoter

Depositor

Andrew Yoo

Article

Victor et al Neuron. 2014 Oct 22;84(2):311-23. doi: 1

Insert

MYT1L (MYT1L Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pMXs-Scl

Plasmid

#131601

Purpose

expression of mouse ScI in mammalian cells

Depositor

Kateri Moore, Filipe Pereira

Article

Pereira et al Cell Stem Cell. 2013 Aug 1;13(2):205-18.

Insert

Scl (Tal1 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pMXs-ms-Sox7

Plasmid

#50780

Purpose

retroviral expression of mouse Sox7

Depositor

Shinya Yamanaka

Article

Nakagawa et al Nat Biotechnol. 2008 Jan;26(1):101-6. Ep

Insert

Sox7 (Sox7 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 28, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pFUW-tetO-Nfil3

Plasmid

#139815

Purpose

doxycycline-inducible expression of mouse Nfil3 in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

Insert

Nfil3 (Nfil3 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE-mCMV

Available since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
GST-TACC3-His6

Plasmid

#69106

Purpose

For bacterial expression of recombinant human TACC3 tagged with GST and His.

Depositor

Stephen Royle

Article

Nixon et al Elife. 2015 Jun 19;4. doi: 10.7554/eLife

Insert

Transforming Acidic Coiled Coil protein 3 (TACC3 Human)

Use

Tags

GST and His6

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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Tet-CA-Src-GFP

Plasmid

#83469

Purpose

Lentiviral vector for doxycycline-inducible expression of constitutively active c-Src in mammalian cells

Depositor

Barry Gumbiner

Article

Kim et al J Cell Biol. 2015 Aug 3;210(3):503-15. d

Insert

Src (SRC Chicken)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Y527F (constitutively active)

Promoter

doxycycline-inducible promoter

Available since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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FUW-TetO-Gata4

Plasmid

#41084

Depositor

Rudolf Jaenisch

Article

Buganim et al Cell Stem Cell. 2012 Sep 7;11(3):373-86.

Insert

Gata4 (Gata4 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TetO + minimal CMV

Available since

Nov. 2, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVX-Tight-Puro_Olig2

Plasmid

#64842

Purpose

A lentiviral vector for the expression of mouse Olig2 under doxycyline control

Depositor

Paul Tesar

Article

Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

Insert

Olig2 (Olig2 Mouse)

Use

Lentiviral

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

May 28, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-rTH-tTA

Plasmid

#133268

Purpose

An AAV genome that expresses tTA from the rat TH promoter

Depositor

Viviana Gradinaru

Article

Robinson et al Elife. 2019 Sep 23;8. pii: 48983. doi: 1

Insert

tTA

Use

AAV

Tags

Expression

Mammalian

Mutation

*(see below)

Promoter

rTH

Available since

Nov. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pLVX-Tight-Puro_ST18

Plasmid

#64844

Purpose

A lentiviral vector for the expression of mouse ST18 under doxycyline control

Depositor

Paul Tesar

Article

Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

Insert

ST18 (St18 Mouse)

Use

Lentiviral

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

May 28, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-ms-Sox18

Plasmid

#50782

Purpose

retroviral expression of mouse Sox18

Depositor

Shinya Yamanaka

Article

Nakagawa et al Nat Biotechnol. 2008 Jan;26(1):101-6. Ep

Insert

Sox18 (Sox18 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 28, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
FUW-TetO-Dmrt1

Plasmid

#41083

Depositor

Rudolf Jaenisch

Article

Buganim et al Cell Stem Cell. 2012 Sep 7;11(3):373-86.

Insert

Dmrt1 (Dmrt1 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TetO + minimal CMV

Available since

Nov. 2, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
GST-TPX2(1-43)

Plasmid

#69107

Purpose

For bacterial expression of human TPX2(1-43aa) tagged with GST.

Depositor

Stephen Royle

Article

Nixon et al Elife. 2015 Jun 19;4. doi: 10.7554/eLife

Insert

Targeting Protein for Xklp2 (TPX2 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Amino acids 1-43 expressed.

Promoter

Available since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
PB-TET-mSox2

Plasmid

#20908

Purpose

piggyBac vector with tet inducible mSox2

Depositor

Andras Nagy

Article

Woltjen et al Nature. 2009 Apr 9;458(7239):766-70. doi

Insert

Sox2 (Sox2 Mouse)

Use

piggybac

Tags

IRES-bGeo

Expression

Mammalian

Mutation

Promoter

Available since

April 23, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-Mllt3

Plasmid

#131604

Purpose

expression of mouse MIIt3 in mammalian cells

Depositor

Kateri Moore, Filipe Pereira

Article

Pereira et al Cell Stem Cell. 2013 Aug 1;13(2):205-18.

Insert

Mllt3 (Mllt3 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-tetO-Klf4

Plasmid

#19764

Depositor

Konrad Hochedlinger

Article

Stadtfeld et al Cell Stem Cell. 2008 Mar 6. 2(3):230-40.

Insert

Klf4 (Klf4 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
Ef1a_LIN28_Ires_Puro

Plasmid

#18921

Depositor

Linzhao Cheng

Article

Mali et al Stem Cells. 2008 Aug . 26(8):1998-2005.

Insert

LIN28 (LIN28A Human)

Use

Cre/Lox and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 4, 2008

Availability

Academic Institutions and Nonprofits only
pLVX-Tight-Puro_Nkx6.2

Plasmid

#64846

Purpose

A lentiviral vector for the expression of mouse Nkx6.2 under doxycyline control

Depositor

Paul Tesar

Article

Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

Insert

Nkx6.2 (Nkx6-2 Mouse)

Use

Lentiviral

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

May 28, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
hGFAP R239C

Plasmid

#169476

Purpose

Single p.Arg239Cys(R239C) mutations was individually inserted by site-directed mutagenesis into the wild-type hGFAP

Depositor

Federico Herrera

Article

Letra-Vilela et al Experimental Results (2020), Volume 1, e

Insert

EGFP-hGFAP (GFAP Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Changed R 239 to C

Promoter

Available since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-Zyxin-Full Length (aa1-564)-EGFP

Plasmid

#187692

Purpose

Visualization of Zyxin

Depositor

Mary Beckerle

Article

Hoffman et al Mol Biol Cell. 2012 May;23(10):1846-59.

Insert

Zyxin (Zyx Mouse)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
PB-Sox17

Plasmid

#60669

Purpose

PiggyBac expression vector encoding murine Sox17 downstream of a doxycycline inducible promoter

Depositor

James Thomson

Article

Vereide et al Stem Cell Reports. 2014 Dec 9;3(6):1043-

Insert

Sox17 (Sox17 Mouse)

Use

Piggybac

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pBabe HcRed Ras

Plasmid

#10678

Depositor

William Hahn

Article

Boehm et al Mol Cell Biol. 2005 Aug . 25(15):6464-74

Insert

Hras V12 (HRAS Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

G12V, constitutively active

Promoter

Available since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only
Tet-FRNK-GFP

Plasmid

#83472

Purpose

Lentiviral vector for doxycycline-inducible expression of FRNK in mammalian cells

Depositor

Barry Gumbiner

Article

Kim et al J Cell Biol. 2015 Aug 3;210(3):503-15. d

Insert

FRNK (PTK2 Chicken)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

FRNK is the non-catalytic c-term domain of FAK ki…

Promoter

doxycycline-inducible promoter

Available since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-C-SLC35B2

Plasmid

#155037

Purpose

Transient expression of EGFP-SLC35B2

Depositor

Yihong Ye

Article

Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

Insert

SLC35B2 (SLC35B2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
mGFAP-Halo

Plasmid

#169474

Purpose

the host vector was obtained by substitution of the EGFP tag from a pEGFP-N3-mGFAP plasmid for a HaloTag using BamHI and NotI restriction sites

Depositor

Federico Herrera

Article

Letra-Vilela et al Experimental Results (2020), Volume 1, e

Insert

GFAP (Gfap Mouse)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Changed R 236 to H

Promoter

Available since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-ms-Sox3

Plasmid

#50779

Purpose

retroviral expression of mouse Sox3

Depositor

Shinya Yamanaka

Article

Nakagawa et al Nat Biotechnol. 2008 Jan;26(1):101-6. Ep

Insert

Sox3 (Sox3 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 28, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
MoFGFR2c-ires neo

Plasmid

#86492

Purpose

mamalian cell expression of FGFR2c

Depositor

David Ornitz

Article

Ornitz et al J Biol Chem. 1996 Jun 21;271(25):15292-7

Insert

FgfrR2 IIIc 15D6 (Fgfr2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Moloney murine leukemia virus LTR

Available since

Feb. 2, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
tetO7_Ef1a_SOX2

Plasmid

#18919

Depositor

Linzhao Cheng

Article

Mali et al Stem Cells. 2008 Aug . 26(8):1998-2005.

Insert

SOX2 (SOX2 Human)

Use

Cre/Lox and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 4, 2008

Availability

Academic Institutions and Nonprofits only
pMMP-puro-Flag-HA-NR6A1

Plasmid

#46833

Depositor

Phil Sharp

Article

Gurtan et al Genes Dev. 2013 Apr 15;27(8):941-54. doi

Insert

NR6A1 (Nr6a1 Mouse)

Use

Retroviral

Tags

Flag-HA, plus 8-amino acid residue linker

Expression

Mammalian

Mutation

None

Promoter

Available since

Nov. 25, 2013

Availability

Academic Institutions and Nonprofits only
pFUW-tetO-Zbtb46

Plasmid

#139811

Purpose

doxycycline-inducible expression of mouse Zbtb46 in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

Insert

Zbtb46 (Zbtb46 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE-mCMV

Available since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-Trib3

Plasmid

#131606

Purpose

expression of mouse Trib3 in mammalian cells

Depositor

Kateri Moore, Filipe Pereira

Article

Pereira et al Cell Stem Cell. 2013 Aug 1;13(2):205-18.

Insert

Trib3 (Trib3 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-ms-Sox1

Plasmid

#50778

Purpose

retroviral expression of mouse Sox1

Depositor

Shinya Yamanaka

Article

Nakagawa et al Nat Biotechnol. 2008 Jan;26(1):101-6. Ep

Insert

Sox1 (Sox1 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 28, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
FN207

Plasmid

#50497

Depositor

Kenneth Yamada

Article

Danen et al J Biol Chem. 1995 Sep 15;270(37):21612-8

Insert

NM_001306129.1 (FN1 Human)

Use

Tags

Expression

Bacterial

Mutation

repeats 6-10

Promoter

T7

Available since

March 24, 2014

Availability

Academic Institutions and Nonprofits only
tetO7_Ef1a_OCT4

Plasmid

#18918

Depositor

Linzhao Cheng

Article

Mali et al Stem Cells. 2008 Aug . 26(8):1998-2005.

Insert

OCT4 (POU5F1 Human)

Use

Cre/Lox and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 4, 2008

Availability

Academic Institutions and Nonprofits only
pMXs-Bex2

Plasmid

#131607

Purpose

expression of mouse Bex2 in mammalian cells

Depositor

Kateri Moore, Filipe Pereira

Article

Pereira et al Cell Stem Cell. 2013 Aug 1;13(2):205-18.

Insert

Bex2 (Bex2 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVX-Tight-Puro_Myt1

Plasmid

#64845

Purpose

A lentiviral vector for the expression of mouse Myt1 under doxycyline control

Depositor

Paul Tesar

Article

Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

Insert

Myt1 (Myt1 Mouse)

Use

Lentiviral

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

May 28, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-hBnvy

Plasmid

#18969

Depositor

Mariano Viapiano

Article

Hu et al J Biol Chem. 2008 Jul 7. ():.

Insert

Brevican uncleavable (BCAN Human)

Use

Tags

Expression

Mammalian

Mutation

Mutation at the ADAMTS cleavage site ESE/SRG: S…

Promoter

Available since

Dec. 12, 2008

Availability

Academic Institutions and Nonprofits only
RNAPII_CTD27-52_K5S

Plasmid

#98679

Purpose

Expresses 6xHis-tagged C-terminal domain of RNA polymerase II with K5S

Depositor

Nicolas Fawzi

Article

Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

Insert

RNA polymerase II C-terminal domain (POLR2A Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

K1838S, K1859S, K1866S, K1873S, K1887S

Promoter

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
mGFAP R236H

Plasmid

#169475

Purpose

Single p.Arg236His(R236H) mutation was individually inserted by site-directed mutagenesis into the wild-type mGFAP

Depositor

Federico Herrera

Article

Letra-Vilela et al Experimental Results (2020), Volume 1, e

Insert

HaloTAG mGFAP (R236H) (Gfap Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Changed R 236 to H

Promoter

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 401 - 450 of 1018 results

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