We narrowed to 8,601 results for: Ott

1. SPLINTR GFP v1 backbone

   Plasmid

   #179777

   Purpose

   SPLINTR backbone vector

   Depositor

   Mark Dawson

   Article

   Fennell et al Nature. 2022 Jan;601(7891):125-131. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBAD_Ub11TCA

   Plasmid

   #174517

   Purpose

   Recoded ubiquitin reporter with TCA replacing codon 11 of wt ubiquitin

   Depositor

   Jason W Chin

   Article

   Robertson et al Science. 2021 Jun 4;372(6546):1057-1062.

   Insert

   Ubiquitin
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Recoded with TCA replacing codon 11 of wt ubiquit…

   Promoter

   araBAD

   Available since

   Sept. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pHR-CMV-TetO2_3C-Avi-His6_IRES-mTurquoise2

   Plasmid

   #113890

   Purpose

   Mammalian (inducible) protein expression, in vivo biotinylation, bicistronic expression of cytosolic mTurquoise2

   Depositor

   A. Radu Aricescu

   Article

   Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   3C-Avi-His6

   Expression

   Mutation

   Promoter

   CMV-MIE-TetO2

   Available since

   Aug. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. p406 – TDH3-Elo3-GFP11-Ura

   Plasmid

   #177734

   Purpose

   ER protein Elo3 with a C-terminal, an HA tag and the eleventh β-strand of GFP in yeast plasmid with URA marker

   Depositor

   Erik Böttger

   Article

   Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

   Insert

   Elo3 (ELO3 Budding Yeast)
   

   Use

   Tags

   GFP11 and HDEL

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pENTR4-Cas9-V5

   Plasmid

   #87905

   Purpose

   Cloning vector

   Depositor

   Nicole Déglon

   Article

   Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

   Insert

   Cas9-V5
   

   Use

   Cloning vector

   Tags

   V5

   Expression

   Mutation

   Human codon optimized

   Promoter

   V5

   Available since

   Sept. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBLO ddd64.1

   Plasmid

   #123121

   Purpose

   E. coli expression vector for catalytically inactivated deltaproteobacteria CasX

   Depositor

   Benjamin Oakes, Jennifer Doudna

   Article

   Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

   Insert

   CasX
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Mutated amino acids in catalytic triad to alanine…

   Promoter

   Available since

   March 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3.1/Hygro(-)-B2-TS5

   Plasmid

   #104354

   Purpose

   Expresses human beta 2 integrin tension sensor

   Depositor

   Pontus Nordenfelt, Timothy Springer

   Article

   Nordenfelt et al Nat Commun. 2016 Oct 10;7:13119. doi: 10

   Insert

   Human integrin b2 with tension sensor (ITGB2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBR322-MCK1.35lux

   Plasmid

   #53368

   Purpose

   expresses luciferase under 1.35 kb muscle creatine kinase promoter/enhancer

   Depositor

   Josephine Nalbantoglu

   Article

   Larochelle et al Gene Ther. 1997 May;4(5):465-72.

   Insert

   muscle creatine kinase promoter/enhancer 1.35kb
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pTet01

   Plasmid

   #78283

   Purpose

   mRFP1 expressed behind an ATc-inducible construct

   Depositor

   Tom Ellis

   Article

   Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

   Insert

   Tet inducible mRFP1
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBabe-Ypet-PBD

    Plasmid

    #24533

    Depositor

    Klaus Hahn

    Article

    Machacek et al Nature. 2009 Sep 3. 461(7260):99-103.

    Insert

    PAK binding domain (PAK1 Human)
    

    Use

    Retroviral

    Tags

    Ypet

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 19, 2010

    Availability

    Academic Institutions and Nonprofits only

11. pENTR221-H1-sgGFP1-U6-sgGFP2-7SK-sgGFP3

    Plasmid

    #87906

    Purpose

    sgRNA targeting GFP

    Depositor

    Nicole Déglon

    Article

    Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

    Insert

    sgGFP1, sgGFP2, sgGFP3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1, U6, 7SK

    Available since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCDNAZeo(-)HSC73

    Plasmid

    #86030

    Purpose

    Expression of Heat shock chaperone 70 in Mammalian cells

    Depositor

    Janice Blum

    Article

    Zhou et al Immunity. 2005 May;22(5):571-81.

    Insert

    Heat-shock cognate protein 73 (HSPA8 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNAintron-CHIKV-Structural polyprotein

    Plasmid

    #215698

    Purpose

    Produces the Chikungunya virus structural polyprotein Capsid-E3-E2-6K-E1

    Depositor

    Melinda Brindley

    Article

    Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

    Insert

    CHIKV structural polyprotein Capsid-E3-E2-6K-E1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pmScarlet-H_alphaTubulin_C1

    Plasmid

    #85046

    Purpose

    In vivo visualization of microtubules (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    Alpha-Tubulin (TUBA1B Human)
    

    Use

    Tags

    mScarlet-H

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMCS.71/2272.Hygro

    Plasmid

    #22731

    Depositor

    Mark Magnuson

    Article

    Chen et al Dis Model Mech. 2011 Jul;4(4):537-47. do

    Insert

    Lox71-FRT-PGK-hygro-FRT-Lox2272
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    This is a basal exchange vector for RMCE. The vec…

    Promoter

    Available since

    Dec. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pOPINK-OTUD3 (OTU+UBA, aa 52-275)

    Plasmid

    #61411

    Purpose

    Expresses human OTUD3 (OTU+UBA domains) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    OTUD3 (OTUD3 Human)
    

    Use

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Deleted aa 1-51 and aa 276-398.

    Promoter

    T7

    Available since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pOPINK-OTUD5 (OTU, aa 171-358)

    Plasmid

    #61415

    Purpose

    Expresses human OTUD5 (OTU domain) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    OTUD5 (OTUD5 Human)
    

    Use

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Deleted aa 1-170 and aa 359-571.

    Promoter

    T7

    Available since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Dendra2-Vinculin-N-21

    Plasmid

    #57749

    Purpose

    Localization: Focal Adhesions, Excitation: 490 / 553, Emission: 507 / 573

    Depositor

    Michael Davidson

    Article

    Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

    Insert

    NM_003373.3 (VCL Human)
    

    Use

    Tags

    Dendra2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 6, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

19. pBabe-CyPet-Rac1

    Plasmid

    #24534

    Depositor

    Klaus Hahn

    Article

    Machacek et al Nature. 2009 Sep 3. 461(7260):99-103.

    Insert

    Rac1 (RAC1 Human)
    

    Use

    Retroviral

    Tags

    CyPet

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2010

    Availability

    Academic Institutions and Nonprofits only

20. p-mCherry2-sgMUC4

    Plasmid

    #198399

    Purpose

    Expresses a guide RNA against a repetitive locus found in the MUC4 gene of human chromosome 3, with mCherry2 reporter

    Depositor

    Sarah McClelland

    Article

    Tovini et al EMBO J. 2023 May 15;42(10):e111587. doi:

    Insert

    MUC4 sgRNA (MUC4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pMXs_FLAG-SFXN1

    Plasmid

    #110634

    Purpose

    Retroviral expression of Flag-SFXN1 in mammalian cells

    Depositor

    David Sabatini

    Article

    Kory et al Science. 2018 Nov 16;362(6416). pii: 362

    Insert

    SFXN1 (SFXN1 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    codon-optimized cDNA (no amino acid mutations)

    Promoter

    Available since

    Nov. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNAintron-CHIKV-nsp1-c3xFLAG

    Plasmid

    #215694

    Purpose

    Produces the Chikungunya virus nonstructural protein 1 with a c-terminal 3xFLAG tag

    Depositor

    Melinda Brindley

    Article

    Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

    Insert

    CHIKV nsp1
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

23. pcDNAintron-CHIKV-nsp2-c3xFLAG

    Plasmid

    #215695

    Purpose

    Produces the Chikungunya virus nonstructural protein 2 with a c-terminal 3xFLAG tag

    Depositor

    Melinda Brindley

    Article

    Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

    Insert

    CHIKV nsp2
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

24. pcDNAintron-CHIKV-nsp4-c3xFLAG

    Plasmid

    #215697

    Purpose

    Produces the Chikungunya virus nonstructural protein 4 with a c-terminal 3xFLAG tag

    Depositor

    Melinda Brindley

    Article

    Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

    Insert

    CHIKV nsp4
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

25. pmScarlet-H_H2A_C1

    Plasmid

    #85052

    Purpose

    In vivo visualization of the nucleus (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    H2A (H2AC16 Human)
    

    Use

    Tags

    mScarlet-H

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMCS.71/2272

    Plasmid

    #22732

    Depositor

    Mark Magnuson

    Article

    Chen et al Dis Model Mech. 2011 Jul;4(4):537-47. do

    Insert

    Lox71-MCS-Lox2272
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    This is a basal exchange vector for RMCE containi…

    Promoter

    Available since

    Dec. 17, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. HES7-NLuc-2A-tdTomato

    Plasmid

    #130932

    Purpose

    Donor vector for tagging human HES7 C-terminus with NanoLuc and tdTomato

    Depositor

    James Thomson

    Article

    Chu et al Cell Rep. 2019 Aug 27;28(9):2247-2255.e5

    Insert

    HES7 (HES7 Human)
    

    Use

    Tags

    NanoLuc and tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pRK5 HA-GST-Presc-mAkt1 (wt)

    Plasmid

    #48805

    Purpose

    expresses wt Akt1

    Depositor

    David Sabatini

    Article

    Kang et al Science. 2013 Jul 26;341(6144):1236566.

    Insert

    Akt1 (Akt1 Mouse)
    

    Use

    Tags

    HA-GST-Presc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

29. pLenti PGK OGT (Neo)

    Plasmid

    #154289

    Purpose

    Lentiviral vector encoding full length wildtype human OGT

    Depositor

    Suzanne Walker

    Article

    Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

    Insert

    O-GlcNAc Transferase (OGT Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. CMV-10xPP7 oligo-library based

    Plasmid

    #158199

    Purpose

    Cassette of 10 different binding sites with high affinity to PP7 phage coat protein, as extracted from the oligo pool experiment.

    Depositor

    Roee Amit

    Article

    Katz et al Nat Commun. 2021 Mar 11;12(1):1576. doi:

    Insert

    10 binding sites of PP7 from the oligo library
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. T-STOP sgRNA-1

    Plasmid

    #83346

    Purpose

    The sgRNA at the stop codon of human Brachyury(T) locus

    Depositor

    James Thomson

    Article

    Chu et al Genome Biol. 2016 Aug 17;17(1):173. doi:

    Insert

    T-STOP sgRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET51b-His-TEV-HOB_(HaloTag7_oligo_binder)

    Plasmid

    #167268

    Purpose

    Overexpression of the HaloTag7 variant HOB (halo-based oligonucleotide binder) in E. coli

    Depositor

    Kai Johnsson

    Article

    Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

    Insert

    HOB
    

    Use

    Tags

    His x10

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pRSET-pr-mEos3.1 (mEosFP3.1-A69T)

    Plasmid

    #99221

    Purpose

    Bacterial expression of the primed conversion capable protein pr-mEos3.1 (mEos3.1-A69T).

    Depositor

    Periklis Pantazis

    Article

    Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

    Insert

    pr-mEos3.1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    A69T for primed conversion applications

    Promoter

    T7

    Available since

    Oct. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. MSCV-miR30_shBrd9_1061-PGK-NeoR-IRES-mCherry

    Plasmid

    #75132

    Purpose

    Retroviral expression plasmid encoding shBrd9_1061 (targeting murine Brd9)

    Depositor

    Christopher Vakoc

    Article

    Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

    Insert

    shBrd9_1061
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 23, 2016

    Availability

    Academic Institutions and Nonprofits only

35. p6346 MSCV-CMV-Flag-HA-Brd4-1-444

    Plasmid

    #32886

    Depositor

    Peter Howley

    Article

    Rahman et al Mol Cell Biol. 2011 Jul . 31(13):2641-52

    Insert

    Brd4 (BRD4 Human)
    

    Use

    Retroviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 4, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. IG510: pMVP (R4-R3) human PDX1

    Plasmid

    #121744

    Purpose

    pMVP R4-R3 entry plasmid, contains human PDX1 for 3- or 4-component MultiSite Gateway Pro assembly

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    Human PDX1 (PDX1 Human)
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pTBL437 AAVS1 EQR sgRNA

    Plasmid

    #126447

    Purpose

    To cut the human AAVS1 locus for integration.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Insert

    spacer against human AAVS1 safe-harbor locus with NGAG PAM
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6

    Available since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. PBLO CasX-gRNA

    Plasmid

    #126419

    Purpose

    E. coli vector for CasX gRNA --- Used for In Vitro Transcription

    Depositor

    Jennifer Doudna

    Article

    Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

    Insert

    casX gRNA
    

    Use

    CRISPR; In vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. phFluc-WT

    Plasmid

    #177714

    Purpose

    Cytosolic firefly luciferase reporter previously described in Shcherbakov et al. 2019

    Depositor

    Erik Böttger

    Article

    Shcherbakov et al Commun Biol. 2019 Oct 17;2:381. doi: 10.

    Insert

    hFluc WT
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Jan. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pENTR1A hu_ncOGT WT (open N-term)

    Plasmid

    #155197

    Purpose

    Entry vector encoding non-tagged O-GlcNAc Transferase with open N-terminus

    Depositor

    Suzanne Walker

    Article

    Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

    Insert

    O-GlcNAc Transferase (OGT Human)
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    WT OGT with no start codon, in-frame with gateway…

    Promoter

    Available since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. LifeAct-mAvicFP1

    Plasmid

    #129512

    Purpose

    Expresses LifeAct-mAvicFP1 in mammalian cells

    Depositor

    Nathan Shaner

    Article

    Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

    Insert

    LifeAct-mAvicFP1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mAvicFP1 is AvicFP1 F64L + A206K

    Promoter

    CMV

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pcDNA3.1/Hygro(-)-B2-TS3

    Plasmid

    #104353

    Purpose

    Expresses human beta 2 integrin tension sensor

    Depositor

    Pontus Nordenfelt, Timothy Springer

    Article

    Nordenfelt et al Nat Commun. 2016 Oct 10;7:13119. doi: 10

    Insert

    Human integrin b2 with tension sensor (ITGB2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    AA1-733 ITGB2 present

    Promoter

    CMV

    Available since

    Jan. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. MSCV-miR30_shBrd9_1061-PGK-NeoR-IRES-GFP

    Plasmid

    #75129

    Purpose

    Retroviral expression plasmid encoding shBrd9_1061 (targeting murine Brd9)

    Depositor

    Christopher Vakoc

    Article

    Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

    Insert

    shBrd9_1061
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 23, 2016

    Availability

    Academic Institutions and Nonprofits only

44. pAAV CB6 FFLuc-miR122

    Plasmid

    #35656

    Depositor

    Phillip Zamore

    Article

    Xie et al Nat Methods. 2012 Mar 4;9(4):403-9. doi:

    Insert

    pri-miR-122 (Mir122 )
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CB

    Available since

    April 26, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pLenti-hPGK-mCherry-SynaptoZip

    Plasmid

    #177317

    Purpose

    Lentiviral expression of the synaptic activity-marker SynaptoZip fused to mCherry driven by hPGK promoter

    Depositor

    Antonio Malgaroli

    Article

    Lamanna et al Sci Rep. 2022 Jun 30;12(1):11055. doi: 1

    Insert

    mCherry-SynaptoZip (Vamp2 Rat)
    

    Use

    Lentiviral

    Tags

    Myc and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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46. JN202: pMVP/SB/Blast-DEST

    Plasmid

    #121864

    Purpose

    pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ Blast selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Sleeping beauty transposon, pmvp gateway destinat…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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47. pOPINK-VCPIP (OTU, aa 25-561)

    Plasmid

    #61583

    Purpose

    Expresses human VCPIP (OTU domain) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    VCPIP (VCPIP1 Human)
    

    Use

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Deleted aa 1-24 and aa 562-1222.

    Promoter

    T7

    Available since

    Dec. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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48. KK501: pMVP (L3-L2) FKBP DD + polyA

    Plasmid

    #121789

    Purpose

    pMVP L3-L2 entry plasmid, contains FKBP DD + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows fusion of C-term FKBP degron domain (stabilized by SHLD1) to gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    FKBP Degron Domain + polyA
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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49. SPLINTR BFP v1 backbone

    Plasmid

    #179779

    Purpose

    SPLINTR backbone vector

    Depositor

    Mark Dawson

    Article

    Fennell et al Nature. 2022 Jan;601(7891):125-131. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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50. NG0750 pCI-TPI-PTC48-4H

    Plasmid

    #65803

    Purpose

    NMD reporter mRNA

    Depositor

    Niels Gehring

    Article

    Boehm et al Cell Rep. 2014 Oct 23;9(2):555-68. doi:

    Insert

    TPI (TPI1 Human)
    

    Use

    Tags

    4H (4 copies of short beta-globin 3'UTR for …

    Expression

    Mammalian

    Mutation

    premature stop codon at 48

    Promoter

    CMV

    Available since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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