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We narrowed to 13,968 results for: SHI

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Showing: 4451 - 4500 of 13968 results

  1. pCAG-EGxxFP

    Plasmid
    #50716
    Purpose
    5’ and 3’ EGFP fragments that shares 482bp were placed under ubiquitous CAG promoter. Used for validation of gRNA sequences by DSB mediated EGFP reconstitution.
    Depositor
    Masahito Ikawa
    Article
    Mashiko et al Sci Rep. 2013 Nov 27;3:3355. doi: 10.103
    Insert
    split EGFP
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Feb. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    50716-plasmid-map-sequence-id-447281

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  2. pM2s2AsR_TS-E10

    Plasmid
    #137944
    Purpose
    E. coli Nissle pMUT2-derived plasmid with ampicillin resistance and constitutive RFP expression and strong temperature sensitive GFP expression
    Depositor
    Neel Joshi
    Article
    Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102
    Inserts
    mRFP1
    mWasabi
    Use
    Synthetic Biology; For use with e. coli nissle in…
    Expression
    Bacterial
    Promoter
    J23101 and pTlpA-C7
    Available Since
    May 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    137944-plasmid-map-sequence-id-331371

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  3. CAG-GFP/cre

    Plasmid
    #49054
    Purpose
    Retroviral vector contains CAG promoter , CMV enhancer and a large synthetic intron that used to allow the ubiquitous expression of GFP-fused Cre recombinase
    Depositor
    Fred Gage
    Article
    Tashiro et al Nat Protoc. 2006;1(6):3049-55.
    Insert
    GFP/Cre
    Use
    Retroviral
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Nov. 4, 2013
    Availability
    Academic Institutions and Nonprofits only
    49054-plasmid-map-sequence-id-490793

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  20. pSLS.492

    Plasmid
    #184957
    Purpose
    Test effect of a1/a2 length on editing rpoB using retron recombineering
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1 RT and recombineering ncRNA, rpoB T1534C, a1/a2 length: 22
    Expression
    Bacterial
    Mutation
    rpoB donor T1534C, a1/a2 length extended to 22 bp
    Promoter
    T7/lac
    Available Since
    Nov. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    184957-plasmid-map-sequence-id-376681

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  35. pGL6

    Plasmid
    #48746
    Purpose
    Expression of luciferase driven by mPer2 promoter region (bases -1128 to +2129 with respect to transcription start site at +1)
    Depositor
    Joseph Takahashi
    Article
    Yoo et al Proc Natl Acad Sci U S A. 2005 Feb 15;10
    Insert
    mPer2 promoter/enhancer region (-1128 to +2129)
    Use
    Luciferase
    Available Since
    Dec. 19, 2013
    Availability
    Academic Institutions and Nonprofits only

  38. pMSCVpuroATT

    Plasmid
    #47539
    Purpose
    Retroviral Gateway destination vector constructed by inserting Reading Frame Cassette A into pMSCVpuro (Clontech) using the Gateway Vector Conversion System (Invitrogen)
    Depositor
    David Weinstock
    Article
    Shindoh et al PLoS One. 2012;7(11):e49201. doi: 10.137
    Type
    Empty backbone
    Use
    Retroviral
    Expression
    Mammalian
    Available Since
    Sept. 25, 2013
    Availability
    Academic Institutions and Nonprofits only

  42. pM1s3ATScsg-Etag

    Plasmid
    #137946
    Purpose
    E. coli Nissle pMUT1-derived plasmid with ampicillin resistance and temperature sensitive curli expression
    Depositor
    Neel Joshi
    Article
    Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102
    Inserts
    Synthetic curli operon
    Synthetic curli operon
    Use
    Synthetic Biology; For use with e. coli nissle in…
    Expression
    Bacterial
    Promoter
    pTlpA36
    Available Since
    April 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    137946-plasmid-map-sequence-id-331396

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  47. HYG-TET-OFF – ubiquitin – LEU – 4FLAG

    Plasmid
    #193315
    Purpose
    Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xFLAG N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.
    Depositor
    Rob Mitra
    Article
    Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102
    Insert
    Ubiquitin - Leucine - 4FLAG N-terminal tagging
    Tags
    4FLAG
    Expression
    Bacterial
    Available Since
    Jan. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    193315-plasmid-map-sequence-id-382367

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  48. pM1s3AsR_TS-C7

    Plasmid
    #137945
    Purpose
    E. coli Nissle pMUT1-derived plasmid with ampicillin resistance and constitutive RFP expression and strong temperature sensitive GFP expression
    Depositor
    Neel Joshi
    Article
    Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102
    Insert
    mRFP1
    Use
    Synthetic Biology; For use with e. coli nissle in…
    Expression
    Bacterial
    Promoter
    J23101
    Available Since
    April 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    137945-plasmid-map-sequence-id-331373

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  49. pKHR5

    Plasmid
    #74593
    Purpose
    an alpha-crystallin::Venus (green lens) reporter cassette flanked by FRT sites and with a single loxP site at the 3’ end was inserted in the middle of pKHR4 mcs. Genbank accession number is KU144823.
    Depositor
    David Grunwald
    Article
    Hoshijima et al Dev Cell. 2016 Mar 21;36(6):654-67. doi:
    Insert
    FRT-alpha-crystallin::Venus-FRT-loxP reporter cassette
    Use
    Cre/Lox; Zebrafish expression
    Promoter
    alpha-crystallin
    Available Since
    April 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    74593-plasmid-map-sequence-id-140738

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Showing: 4451 - 4500 of 13968 results